Team:ETH Zurich/modeling/whole

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(With Leakiness)
(With Leakiness)
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[[File:ETHZ_Leakiness.png|center|800px|thumb|The four cases for the whole cell with basal leakiness]]
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[[File:ETHZ_Leakiness.png|center|800px|thumb|'''Figure 6''' The four cases for the whole cell with basal leakiness]]
From our experimental data, we observed some basal leakiness for P<sub>Lux</sub> and P<sub>Las</sub> even after using riboregulators. From the model we see that, this small basal leakiness is amplified downstream. The basal leakiness results in production of integrases which further act on the XOR module and cause the switching of the terminator. Thus, there is GFP and LasI produced, and the LasI produced further catalyses the production of more LasAHL. Thus, we observe some GFP even without inputs.   
From our experimental data, we observed some basal leakiness for P<sub>Lux</sub> and P<sub>Las</sub> even after using riboregulators. From the model we see that, this small basal leakiness is amplified downstream. The basal leakiness results in production of integrases which further act on the XOR module and cause the switching of the terminator. Thus, there is GFP and LasI produced, and the LasI produced further catalyses the production of more LasAHL. Thus, we observe some GFP even without inputs.   
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<!--[[File:ETHZ_00TerminatorwithLeakiness.png|center|500px|thumb|'''Figure 6''' No inputs and only basal leakiness]]-->
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<!--[[File:ETHZ_00TerminatorwithLeakiness.png|center|500px|thumb|'''Figure 7''' No inputs and only basal leakiness]]-->
<!--The figure above summarizes the predicted effect of basal leakiness on the flipping of the terminator. The basal leakiness results in production of Bxb1 and ΦC31 which result in flipping of the terminator. In this case, since the cell produces LasI there is increased production of LasAHL. The LasAHL produced induces the production of ΦC31 which further causes flipping of all terminators flanked by the ΦC31 sites. Thus, by 200 minutes almost all ΦC31 sites are inactive and the cell will stay ON. -->
<!--The figure above summarizes the predicted effect of basal leakiness on the flipping of the terminator. The basal leakiness results in production of Bxb1 and ΦC31 which result in flipping of the terminator. In this case, since the cell produces LasI there is increased production of LasAHL. The LasAHL produced induces the production of ΦC31 which further causes flipping of all terminators flanked by the ΦC31 sites. Thus, by 200 minutes almost all ΦC31 sites are inactive and the cell will stay ON. -->
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[[File:ETHZ_XORWholeCellwithLeakiness.png|center|800px|thumb|Predicted XOR behaviour for the whole cell model with leakiness]]
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[[File:ETHZ_XORWholeCellwithLeakiness.png|center|800px|thumb|'''Figure 8''' Predicted XOR behaviour for the whole cell model with leakiness]]
=== With Leakiness and Crosstalk ===  
=== With Leakiness and Crosstalk ===  

Revision as of 23:36, 17 October 2014

iGEM ETH Zurich 2014