Team:ETH Zurich/modeling/qs

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== Alternate Design ==
== Alternate Design ==
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As cross-talk is a burning issue in quorum sensing, we thought about a theoretical solution. [https://2014.igem.org/Team:Edinburgh Edinburgh iGEM team 2014]also worked on communication between ''E. coli''. They developed new communication channels via metabolic wiring. By assuming that quorum sensing molecules would not cross-talk with metabolites, we used their idea to develop a model on molecular level. The idea is finally to combine our [https://2014.igem.org/Team:ETH_Zurich/modeling#Alternate_Design whole-cell model] and their idea on metabolites.
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As cross-talk is a burning issue in quorum sensing, we thought about a theoretical solution. [https://2014.igem.org/Team:Edinburgh Edinburgh iGEM team 2014] also worked on communication between ''E. coli''. They developed new communication channels via metabolic wiring. By assuming that quorum sensing molecules would not cross-talk with metabolites, we used their idea to develop a model on the molecular level. The idea is finally to combine our [https://2014.igem.org/Team:ETH_Zurich/modeling#Alternate_Design whole-cell model] and their idea on metabolites.
Metabolic wiring is based on the fact that when a resource is at disposal, a cell will produce an enzyme to break it down to smaller pieces. There is often a chain of metabolites. Using the fact that metabolites can diffuse through the membrane, this can allow communication (for more information, please see the [https://2014.igem.org/Team:Edinburgh Edinburgh iGEM team 2014] wiki).
Metabolic wiring is based on the fact that when a resource is at disposal, a cell will produce an enzyme to break it down to smaller pieces. There is often a chain of metabolites. Using the fact that metabolites can diffuse through the membrane, this can allow communication (for more information, please see the [https://2014.igem.org/Team:Edinburgh Edinburgh iGEM team 2014] wiki).
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=== Application to our project ===
=== Application to our project ===
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Populations of bacteria will grow into a medium, which provide them metabolite A. The metabolite B will serve as communicating signal, like LasAHL or LuxAHL. It will be an input for the logic construct. As soon as metabolite B is being made available to the cell, the cell will produce the input for the logic construct.
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Populations of bacteria will grow into a medium, which provides them metabolite A. The metabolite B will serve as communicating signal, like LasAHL or LuxAHL. It will be an input for the logic construct. As soon as metabolite B is being made available to the cell, the cell will produce the input for the logic construct.
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Moreover, one type of cell will produce B as output. Therefore, the output of the logic gate signal will correspond to the production (or absence of production) of the enzyme Enz, so that the A contained in the medium can be transformed to B. Enz will play the same role as LasI or LuxI in our original model.
Moreover, one type of cell will produce B as output. Therefore, the output of the logic gate signal will correspond to the production (or absence of production) of the enzyme Enz, so that the A contained in the medium can be transformed to B. Enz will play the same role as LasI or LuxI in our original model.

Revision as of 05:34, 17 October 2014

iGEM ETH Zurich 2014