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Team:ETH Zurich/labblog/20140825meet
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<html><article class="mix carousel meetings" id="week14" date="20140825"></html> | <html><article class="mix carousel meetings" id="week14" date="20140825"></html> | ||
| - | == Week 14 == | + | == Week 14 : Diffusion experiments started, simulated with Comsol, and fitting of quorum sensing dynamics == |
==== Monday, August 25th ==== | ==== Monday, August 25th ==== | ||
| - | * We carried diffusion experiment with different designs : punched PDMS, liquid, beads. For every design we added high concentrations of pure AHL in the sender well, so cells are glowing a lot. However because of this high concentration, diffusion is very high and at a given AHL concentration, receiving cells are glowing at the same time for all 4 channel lengths. Check <html> <a id="insidelink" | + | * We carried diffusion experiment with different designs : punched PDMS, liquid, beads. For every design we added high concentrations of pure AHL in the sender well, so cells are glowing a lot. However because of this high concentration, diffusion is very high and at a given AHL concentration, receiving cells are glowing at the same time for all 4 channel lengths. Check <html> <a id="insidelink" targetid="diffusionexp" href="https://2014.igem.org/Team:ETH_Zurich/blog#diffusionexp"> the corresponding article </a> </html> for more details. |
*We have a Comsol simulation for these experiments that explains why at this concentration of AHL (1mM), all 4 wells glow at the same time irrespectively of channel length. Check <html> <a id="insidelink" targetid="diffcomsol" href="https://2014.igem.org/Team:ETH_Zurich/blog#diffcomsol"> this article </a> </html> for more details. | *We have a Comsol simulation for these experiments that explains why at this concentration of AHL (1mM), all 4 wells glow at the same time irrespectively of channel length. Check <html> <a id="insidelink" targetid="diffcomsol" href="https://2014.igem.org/Team:ETH_Zurich/blog#diffcomsol"> this article </a> </html> for more details. | ||
* We could fit degradation rates of external, internal AHL and GFP thanks to quorum sensing dynamic curves. | * We could fit degradation rates of external, internal AHL and GFP thanks to quorum sensing dynamic curves. | ||
| - | + | Check <html> <a id="insidelink" targetid="qsdynamics" href="https://2014.igem.org/Team:ETH_Zurich/blog#qsdynamics"> the corresponding article </a> </html> for more details. | |
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{{:Team:ETH_Zurich/tpl/topbutton|silver}} | {{:Team:ETH_Zurich/tpl/topbutton|silver}} | ||
Latest revision as of 19:38, 11 October 2014
Week 14 : Diffusion experiments started, simulated with Comsol, and fitting of quorum sensing dynamics
Monday, August 25th
- We carried diffusion experiment with different designs : punched PDMS, liquid, beads. For every design we added high concentrations of pure AHL in the sender well, so cells are glowing a lot. However because of this high concentration, diffusion is very high and at a given AHL concentration, receiving cells are glowing at the same time for all 4 channel lengths. Check the corresponding article for more details.
- We have a Comsol simulation for these experiments that explains why at this concentration of AHL (1mM), all 4 wells glow at the same time irrespectively of channel length. Check this article for more details.
- We could fit degradation rates of external, internal AHL and GFP thanks to quorum sensing dynamic curves.
Check the corresponding article for more details.
