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Team:ETH Zurich/labblog/20140825meet
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| - | Diffusion experiment with agar. Check <html> <a class="all" id="insidelink" href="https://2014.igem.org/Team:ETH_Zurich/blog#diffusionexp"> the corresponding article </a> </html> for more details. | + | Diffusion experiment with agar. Click on the picture to watch the video. Check <html> <a class="all" id="insidelink" href="https://2014.igem.org/Team:ETH_Zurich/blog#diffusionexp"> the corresponding article </a> </html> for more details. |
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[[File:ETH_Zurich_Diffusion2.JPG|center|500px|link=https://2014.igem.org/Team:ETH_Zurich/Video1]] | [[File:ETH_Zurich_Diffusion2.JPG|center|500px|link=https://2014.igem.org/Team:ETH_Zurich/Video1]] | ||
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| - | Diffusion experiment with liquid. Check <html> <a class="all" id="insidelink" href="https://2014.igem.org/Team:ETH_Zurich/blog#diffusionexp"> the corresponding article </a> </html> for more details. | + | Diffusion experiment with liquid. Click on the picture to watch the video. Check <html> <a class="all" id="insidelink" href="https://2014.igem.org/Team:ETH_Zurich/blog#diffusionexp"> the corresponding article </a> </html> for more details. |
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[[File:ETH_Zurich_Diffusion3.JPG|center|500px|link=https://2014.igem.org/Team:ETH_Zurich/Video1]] | [[File:ETH_Zurich_Diffusion3.JPG|center|500px|link=https://2014.igem.org/Team:ETH_Zurich/Video1]] | ||
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| - | Diffusion experiment with beads. Check <html> <a class="all" id="insidelink" href="https://2014.igem.org/Team:ETH_Zurich/blog#diffusionexp"> the corresponding article </a> </html> for more details. | + | Diffusion experiment with beads. Click on the picture to watch the video. Check <html> <a class="all" id="insidelink" href="https://2014.igem.org/Team:ETH_Zurich/blog#diffusionexp"> the corresponding article </a> </html> for more details. |
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[[File:ETH_Zurich_Diffusion4.JPG|center|500px|link=https://2014.igem.org/Team:ETH_Zurich/Video1]] | [[File:ETH_Zurich_Diffusion4.JPG|center|500px|link=https://2014.igem.org/Team:ETH_Zurich/Video1]] | ||
Revision as of 09:39, 8 September 2014
Week 14
Monday, August 25th
- We carried diffusion experiment with different designs : punched PDMS, liquid, beads. For every design we added high concentrations of pure AHL in the sender well, so cells are glowing a lot. However because of this high concentration, diffusion is very high and at a given AHL concentration, receiving cells are glowing at the same time for all 4 channel lengths.
Diffusion experiment with agar. Click on the picture to watch the video. Check the corresponding article for more details.
Diffusion experiment with liquid. Click on the picture to watch the video. Check the corresponding article for more details.
Diffusion experiment with beads. Click on the picture to watch the video. Check the corresponding article for more details.
- We have a Comsol simulation for these experiments that explains why at this concentration of AHL (1mM), all 4 wells glow at the same time irrespectively of channel length.
- We could fit degradation rates of external, internal AHL and GFP thanks to quorum sensing dynamic curves.
