http://2014.igem.org/wiki/index.php?title=Team:ETH_Zurich/labblog/20140818mod&feed=atom&action=historyTeam:ETH Zurich/labblog/20140818mod - Revision history2024-03-28T23:30:04ZRevision history for this page on the wikiMediaWiki 1.16.5http://2014.igem.org/wiki/index.php?title=Team:ETH_Zurich/labblog/20140818mod&diff=194964&oldid=prevClormeau at 19:20, 11 October 20142014-10-11T19:20:11Z<p></p>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>== Fitting quorum sensing Hill functions parameters == </div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>== Fitting quorum sensing Hill functions parameters == </div></td></tr>
</table>Clormeauhttp://2014.igem.org/wiki/index.php?title=Team:ETH_Zurich/labblog/20140818mod&diff=194878&oldid=prevClormeau: /* Quorum sensing parameter fitting */2014-10-11T19:08:40Z<p><span class="autocomment">Quorum sensing parameter fitting</span></p>
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<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>== <del class="diffchange diffchange-inline">Quorum </del>sensing <del class="diffchange diffchange-inline">parameter fitting </del>== </div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>== <ins class="diffchange diffchange-inline">Fitting quorum </ins>sensing <ins class="diffchange diffchange-inline">Hill functions parameters </ins>== </div></td></tr>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>==== Wednesday, August 18th ====</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>==== Wednesday, August 18th ====</div></td></tr>
</table>Clormeauhttp://2014.igem.org/wiki/index.php?title=Team:ETH_Zurich/labblog/20140818mod&diff=192980&oldid=prevClormeau at 15:13, 11 October 20142014-10-11T15:13:24Z<p></p>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>== Quorum sensing parameter fitting == </div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>== Quorum sensing parameter fitting == </div></td></tr>
</table>Clormeauhttp://2014.igem.org/wiki/index.php?title=Team:ETH_Zurich/labblog/20140818mod&diff=192615&oldid=prevClormeau at 14:31, 11 October 20142014-10-11T14:31:57Z<p></p>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>== Quorum sensing parameter fitting == </div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>== Quorum sensing parameter fitting == </div></td></tr>
</table>Clormeauhttp://2014.igem.org/wiki/index.php?title=Team:ETH_Zurich/labblog/20140818mod&diff=172483&oldid=prevClormeau at 01:42, 8 October 20142014-10-08T01:42:35Z<p></p>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>== Quorum sensing parameter fitting == </div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>== Quorum sensing parameter fitting == </div></td></tr>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>These results are promising and we look forward to fit the dynamic data of this system.</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>These results are promising and we look forward to fit the dynamic data of this system.</div></td></tr>
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<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>{{:Team:ETH Zurich/tpl/topbutton}}</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>{{:Team:ETH Zurich/tpl/topbutton<ins class="diffchange diffchange-inline">|blue}}</ins></div></td></tr>
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</table>Clormeauhttp://2014.igem.org/wiki/index.php?title=Team:ETH_Zurich/labblog/20140818mod&diff=172283&oldid=prevClormeau at 23:36, 7 October 20142014-10-07T23:36:43Z<p></p>
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<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>The experimental design also allowed us to detect eventual cross-talk with other signaling molecules, the one corresponding to the las system and the one corresponding to the rhl system. No cross-talk was observed with the signaling molecule of the rhl system. However, the las system signaling molecule activated the Lux promoter. We chose to model this cross-talk, which corresponds to the induction of a promoter, by an Hill function. This fitting allowed us to verify that the Hill function was adapted to describe a cross-talk phenomena. The derived parameters indicated that the concentration in the las signaling molecule had to be high in order to have an effect on our system (It was two orders of magnitude away from the needed concentration of AHL to activate the system). Nevertheless, once activated, the effects of these crosstalk are not to be neglected. Check <html> <a <del class="diffchange diffchange-inline">class</del>="<del class="diffchange diffchange-inline">all</del>" id="insidelink" href="https://2014.igem.org/Team:ETH_Zurich/blog#cross-talk"> the following article </a> </html> for more information on the experimental setting.</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>The experimental design also allowed us to detect eventual cross-talk with other signaling molecules, the one corresponding to the las system and the one corresponding to the rhl system. No cross-talk was observed with the signaling molecule of the rhl system. However, the las system signaling molecule activated the Lux promoter. We chose to model this cross-talk, which corresponds to the induction of a promoter, by an Hill function. This fitting allowed us to verify that the Hill function was adapted to describe a cross-talk phenomena. The derived parameters indicated that the concentration in the las signaling molecule had to be high in order to have an effect on our system (It was two orders of magnitude away from the needed concentration of AHL to activate the system). Nevertheless, once activated, the effects of these crosstalk are not to be neglected. Check <html> <a <ins class="diffchange diffchange-inline">targetid</ins>="<ins class="diffchange diffchange-inline">cross-talk</ins>" id="insidelink" href="https://2014.igem.org/Team:ETH_Zurich/blog#cross-talk"> the following article </a> </html> for more information on the experimental setting.</div></td></tr>
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</table>Clormeauhttp://2014.igem.org/wiki/index.php?title=Team:ETH_Zurich/labblog/20140818mod&diff=172281&oldid=prevClormeau at 23:36, 7 October 20142014-10-07T23:36:15Z<p></p>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>== Quorum sensing parameter fitting == </div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>== Quorum sensing parameter fitting == </div></td></tr>
</table>Clormeauhttp://2014.igem.org/wiki/index.php?title=Team:ETH_Zurich/labblog/20140818mod&diff=172069&oldid=prevClormeau at 22:02, 7 October 20142014-10-07T22:02:02Z<p></p>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>== Quorum sensing parameter fitting == </div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>== Quorum sensing parameter fitting == </div></td></tr>
</table>Clormeauhttp://2014.igem.org/wiki/index.php?title=Team:ETH_Zurich/labblog/20140818mod&diff=109842&oldid=prevClormeau at 11:55, 9 September 20142014-09-09T11:55:26Z<p></p>
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</table>Clormeauhttp://2014.igem.org/wiki/index.php?title=Team:ETH_Zurich/labblog/20140818mod&diff=109841&oldid=prevClormeau at 11:55, 9 September 20142014-09-09T11:55:17Z<p></p>
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</table>Clormeau