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Team:ETH Zurich/lab/biobrick/used1
From 2014.igem.org
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<I>ETH Zurich 2014</I> | <I>ETH Zurich 2014</I> | ||
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= Characterization of the promoter's basal leakiness to LuxR in the absence of 3OC6-HSL = | = Characterization of the promoter's basal leakiness to LuxR in the absence of 3OC6-HSL = | ||
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Other regulatory proteins, like LasR, bind to different HSL and activates the promoter | Other regulatory proteins, like LasR, bind to different HSL and activates the promoter | ||
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| + | == Results == | ||
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| + | = Characterization of the promoter's sensitivity to 3OC6-HSL depending on LuxR concentration = | ||
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| + | == Background information == | ||
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| + | === Systems considered === | ||
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| + | === Modeling promoter's sensitivity === | ||
== Results == | == Results == | ||
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Revision as of 10:06, 16 October 2014
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<partinfo>BBa_R0062 AddReview 4</partinfo> ETH Zurich 2014 |
Characterization of the promoter's basal leakiness to LuxR in the absence of 3OC6-HSLBackground informationSystems consideredModeling leakinessResultsCharacterization of two-level crosstalk on the promoterBackground informationSystem consideredModeling crosstalkFirst-order crosstalkFirst Level crosstalk: LuxR binds to different HSL and activates the promoterSecond Level crosstalk: other regulatory proteins, like LasR, bind to their natural HSL substrate and activates the promoterSecond order crosstalk: Combination of both cross-talk levelsOther regulatory proteins, like LasR, bind to different HSL and activates the promoter ResultsCharacterization of the promoter's sensitivity to 3OC6-HSL depending on LuxR concentrationBackground informationSystems consideredModeling promoter's sensitivityResults |
