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Team:ETH Zurich/lab/biobrick/used1
From 2014.igem.org
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=== Modeling crosstalk === | === Modeling crosstalk === | ||
| - | == First- | + | == First-order crosstalk == |
| - | == | + | === First Level crosstalk: LuxR binds to different HSL and activates the promoter === |
| - | === | + | === Second Level crosstalk: other regulatory proteins, like LasR, bind to their natural HSL substrate and activates the promoter === |
| - | == | + | == Second order crosstalk: Combination of both cross-talk levels == |
| - | + | Other regulatory proteins, like LasR, bind to different HSL and activates the promoter | |
== Results == | == Results == | ||
|}; | |}; | ||
Revision as of 10:05, 16 October 2014
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<partinfo>BBa_R0062 AddReview 4</partinfo> ETH Zurich 2014 |
Characterization of the promoter's sensitivity to 3OC6-HSL depending on LuxR concentrationBackground informationSystems consideredModeling promoter's sensitivityResultsCharacterization of the promoter's basal leakiness to LuxR in the absence of 3OC6-HSLBackground informationSystems consideredModeling leakinessResultsCharacterization of two-level crosstalk on the promoterBackground informationSystem consideredModeling crosstalkFirst-order crosstalkFirst Level crosstalk: LuxR binds to different HSL and activates the promoterSecond Level crosstalk: other regulatory proteins, like LasR, bind to their natural HSL substrate and activates the promoterSecond order crosstalk: Combination of both cross-talk levelsOther regulatory proteins, like LasR, bind to different HSL and activates the promoter Results |
