Team:ETH Zurich/lab/biobrick/used1

From 2014.igem.org

(Difference between revisions)

Revision as of 08:34, 16 October 2014

<partinfo>BBa_R0062 AddReview 4</partinfo> ETH Zurich 2014

Contents

Characterization of the promoter's sensitivity to 3OC6-HSL depending on LuxR concentration

Background information

Systems considered

Modeling promoter's sensitivity

Results

Characterization of the promoter's basal leakiness to LuxR in the absence of 3OC6-HSL

Background information

Systems considered

Modeling leakiness

Results

Characterization of two-level crosstalk on the promoter

Background information

System considered

Modeling crosstalk

First-level crosstalk: LuxR binds to different HSL and activates the promoter

Second-level crosstalk: other regulatory proteins, like LasR, bind to their natural HSL substrate and activates the promoter

Background information

Observations

Combination of both levels: other regulatory proteins, like LasR, bind to different HSL and activates the promoter

Results

;