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Team:ETH Zurich/lab/bead
From 2014.igem.org
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| - | |Bacteria are resuspended and diluted in NaCl-solution (0.9 % in | + | |Bacteria are resuspended and diluted in NaCl-solution (0.9 % in H<sub>2</sub>O) so as to achieve the desired cell density. The resuspension was added to alginate (2.5%) to reach a alginate concentration of 2%. The viscous solution is filled into a syringe containing an appropriate needle device. Droplets should fall form a height of approximately 30 cm into a CaCl<sub>2</sub> solution. |
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|[[File:ETH2014_BeadsProduction.jpg|300px]] | |[[File:ETH2014_BeadsProduction.jpg|300px]] | ||
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| - | |A more detailed protocol can be found [https://2014.igem.org/Team:ETH_Zurich/lab/protocols here] | + | |A more detailed protocol can be found [https://2014.igem.org/Team:ETH_Zurich/lab/protocols here.] |
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Revision as of 16:12, 15 October 2014
Beads
Overview
Alginate beads are commonly used for immobilization of macromolecules and cells, however, they are also applied for pharmaceutical purposes such as drug delivery. The polymer
Production
| Bacteria are resuspended and diluted in NaCl-solution (0.9 % in H2O) so as to achieve the desired cell density. The resuspension was added to alginate (2.5%) to reach a alginate concentration of 2%. The viscous solution is filled into a syringe containing an appropriate needle device. Droplets should fall form a height of approximately 30 cm into a CaCl2 solution. |
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| A more detailed protocol can be found here. |
Loading the Chip
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| The beads are better visible in liquid when light shines from the side. So it's easier to load the the chip with help of a cell phone light. |
Features
