Team:ETH Zurich/data
From 2014.igem.org
Data Page
Gene Circuit
For more information about the parts used in this circuit click on the parts in the picture that link to the registry.
Click here to see in action how the circuit responds to different inputs.
Our Favorite New Parts
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Used and Characterized Pre-Existing Parts
- Experience - Promoter (LuxR & 3OC6-HSL regulated), BBa_R0062: the promoter's EC50 could be shown to be dependent on the amount of regulator LuxR (BBa_C0062) in the system by using the three different constitutive promoters BBa_J23100, BBa_J23109, and BBa_J23111 for the production of LuxR. The amount of regulator also demonstrated an influence on the promoter's basal expression or leakiness. Additionally, it could be determined that the promoter can also be activated by the regulators LasR (BBa_C0179) and RhlR (C0171) thus showing crosstalk with these two quorum sensing systems.
- Experience - Promoter (LasR & 3OC12-HSL regulated), BBa_R0079: it could be shown that this promoter, which is usually activated by the regulator LasR (BBa_C0179) can also be activated by the regulators LuxR (BBa_C0062) and RhlR (C0171). Therefore, it shows crosstalk with these two quorum sensing systems.
- Experience - Promoter (RhlR & C4-HSL regulated), BBa_R0071: the amount of regulator RhlR (C0171) demonstrated an influence on the promoter's basal expression or leakiness as well as on the steepness of the response. This was investigated using two different RBSes controlling the expression level of RhlR, i.e. the RBS B0034 and a RBS optimized using the Salis Lab RBS Calculator[13].
Characterized new parts
- there