Team:ETH Zurich/data

From 2014.igem.org

(Difference between revisions)
(Our Favorite New Characterized Parts)
(Used and Characterized Pre-Existing Parts)
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==Used and Characterized Pre-Existing Parts==
==Used and Characterized Pre-Existing Parts==
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#[http://parts.igem.org/Part:BBa_R0062:Experience Experience] - '''Promoter (LuxR/3OC6-HSL regulated), BBa_R0062:''' the promoter's EC<sub>50</sub> could be shown to be dependent on the amount of regulator [http://parts.igem.org/Part:BBa_C0062:Experience LuxR (BBa_C0062)] in the system by using the three different constitutive promoters [http://parts.igem.org/Part:BBa_J23100:Experience BBa_J23100], [http://parts.igem.org/Part:BBa_J23109:Experience BBa_J23109], and [http://parts.igem.org/Part:BBa_J23111:Experience BBa_J23111] for the production of LuxR. The amount of regulator also demonstrated an influence on the promoter's basal expression or leakiness. Additionally, it could be determined that the promoter can also be activated by the regulators [http://parts.igem.org/Part:BBa_C0179:Experience LasR (BBa_C0179)] and [http://parts.igem.org/Part:BBa_C0171:Experience RhlR (C0171)] thus showing crosstalk with these two quorum sensing systems. <html><br><br></html>
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#[http://parts.igem.org/Part:BBa_R0062:Experience Experience] - '''Promoter (LuxR/3OC6-HSL regulated), BBa_R0062:''' the promoter's EC<sub>50</sub> could be shown to be dependent on the amount of regulator [http://parts.igem.org/Part:BBa_C0062:Experience LuxR (BBa_C0062)] in the system by using the three different constitutive promoters [http://parts.igem.org/Part:BBa_J23100:Experience BBa_J23100], [http://parts.igem.org/Part:BBa_J23109:Experience BBa_J23109], and [http://parts.igem.org/Part:BBa_J23111:Experience BBa_J23111] for the production of LuxR. The amount of regulator also demonstrated an influence on the promoter's basal expression or leakiness. Additionally, [[Team:ETH_Zurich/expresults#Quorum_Sensing|it could be determined]] that the promoter can also be activated by the regulators [http://parts.igem.org/Part:BBa_C0179:Experience LasR (BBa_C0179)] and [http://parts.igem.org/Part:BBa_C0171:Experience RhlR (C0171)] thus showing crosstalk with these two quorum sensing systems. <html><br><br></html>
#[http://parts.igem.org/Part:BBa_R0079:Experience Experience] - '''Promoter (LasR/3OC12-HSL regulated), BBa_R0079:''' it could be shown that this promoter is not only activated by the regulator [http://parts.igem.org/Part:BBa_C0179:Experience LasR (BBa_C0179)], but also be activated by the regulators [http://parts.igem.org/Part:BBa_C0062:Experience LuxR (BBa_C0062)] and [http://parts.igem.org/Part:BBa_C0171:Experience RhlR (C0171)]. Therefore, it shows crosstalk with these two quorum sensing systems.<html><br><br></html>
#[http://parts.igem.org/Part:BBa_R0079:Experience Experience] - '''Promoter (LasR/3OC12-HSL regulated), BBa_R0079:''' it could be shown that this promoter is not only activated by the regulator [http://parts.igem.org/Part:BBa_C0179:Experience LasR (BBa_C0179)], but also be activated by the regulators [http://parts.igem.org/Part:BBa_C0062:Experience LuxR (BBa_C0062)] and [http://parts.igem.org/Part:BBa_C0171:Experience RhlR (C0171)]. Therefore, it shows crosstalk with these two quorum sensing systems.<html><br><br></html>
#[http://parts.igem.org/Part:BBa_I14017:Experience Experience] - '''Promoter (RhlR/C4-HSL regulated), BBa_I14017:''' the amount of regulator [http://parts.igem.org/Part:BBa_C0171:Experience RhlR (C0171)] had an influence on the promoter's basal expression level or leakiness as well as on the steepness of the response. This was investigated using two different RBSes controlling the production level of RhlR, i.e. the RBS [http://parts.igem.org/Part:BBa_B0034 B0034] and a RBS optimized for ''rhlR'' using the [http://salis.psu.edu/software/RBSLibraryCalculatorSearchMode Salis Lab RBS Calculator]<sup>[[Team:ETH_Zurich/project/references#refSalis|[13]]]</sup>.<html><br><br></html>
#[http://parts.igem.org/Part:BBa_I14017:Experience Experience] - '''Promoter (RhlR/C4-HSL regulated), BBa_I14017:''' the amount of regulator [http://parts.igem.org/Part:BBa_C0171:Experience RhlR (C0171)] had an influence on the promoter's basal expression level or leakiness as well as on the steepness of the response. This was investigated using two different RBSes controlling the production level of RhlR, i.e. the RBS [http://parts.igem.org/Part:BBa_B0034 B0034] and a RBS optimized for ''rhlR'' using the [http://salis.psu.edu/software/RBSLibraryCalculatorSearchMode Salis Lab RBS Calculator]<sup>[[Team:ETH_Zurich/project/references#refSalis|[13]]]</sup>.<html><br><br></html>

Revision as of 15:17, 17 October 2014

iGEM ETH Zurich 2014