Team:ETH Zurich/data

From 2014.igem.org

(Difference between revisions)
(Our Favorite New Parts)
Line 96: Line 96:
==Our Favorite New Parts==
==Our Favorite New Parts==
#[http://parts.igem.org/Part:BBa_K1541000 Main Page] - '''Riboregulated Promoter (LuxR/3OC6-HSL, RR12y), BBa_K1541000:''' this riboregulated promoter construct contains the quorum sensing promoter [http://parts.igem.org/Part:BBa_R0062 BBa_R0062] which can be activated in presence of [http://parts.igem.org/Part:BBa_C0062:Experience LuxR (BBa_C0062)] and 3OC6-HSL (the product of the enzyme [http://parts.igem.org/Part:BBa_C0161 LuxI (BBa_C0161)]). However, this promoter by itself shows high leakiness. Together with the ribogegulator 12y<sup>[[Team:ETH_Zurich/project/references#refCallura|[18]]]</sup> the leakiness could be reduced by almost two orders of magnitude and the ON/OFF ratio increased approximately 6 times.<html><br><br></html>
#[http://parts.igem.org/Part:BBa_K1541000 Main Page] - '''Riboregulated Promoter (LuxR/3OC6-HSL, RR12y), BBa_K1541000:''' this riboregulated promoter construct contains the quorum sensing promoter [http://parts.igem.org/Part:BBa_R0062 BBa_R0062] which can be activated in presence of [http://parts.igem.org/Part:BBa_C0062:Experience LuxR (BBa_C0062)] and 3OC6-HSL (the product of the enzyme [http://parts.igem.org/Part:BBa_C0161 LuxI (BBa_C0161)]). However, this promoter by itself shows high leakiness. Together with the ribogegulator 12y<sup>[[Team:ETH_Zurich/project/references#refCallura|[18]]]</sup> the leakiness could be reduced by almost two orders of magnitude and the ON/OFF ratio increased approximately 6 times.<html><br><br></html>
-
#[http://parts.igem.org/Part:BBa_K1541006 Main Page] - '''sfGFP under Riboregulated Promoter (LuxR/3OC6-HSL, RR12y), BBa_K1541006:''' .
+
#[http://parts.igem.org/Part:BBa_K1541006 Main Page] - '''sfGFP under Riboregulated Promoter (LuxR/3OC6-HSL, RR12y), BBa_K1541006:''' this part contains the same riboregulated promoter system as the part [http://parts.igem.org/Part:BBa_K1541000 BBa_K1541000] succeeded by the gene for sfGFP. This construct was used to characterize the properties of the riboregulated quorum sensing promoter [http://parts.igem.org/Part:BBa_K1541000 BBa_K1541000] and can be used in a 3OC6-HSL sensor where low leakiness is desirable.
<html></article></html>
<html></article></html>

Revision as of 20:49, 12 October 2014

iGEM ETH Zurich 2014