"
Team:ETH Zurich/data
From 2014.igem.org
(Difference between revisions)
(→Used and Characterized Pre-Existing Parts) |
(→Used and Characterized Pre-Existing Parts) |
||
| Line 24: | Line 24: | ||
#[http://parts.igem.org/Part:BBa_R0071:Experience Experience] - '''Promoter (RhlR & C4-HSL regulated), BBa_R0071:''' the amount of regulator [http://parts.igem.org/Part:BBa_C0171:Experience RhlR (C0171)] demonstrated an influence on the promoter's basal expression or leakiness as well as on the steepness of the response. This was investigated using two different RBSes controlling the expression level of RhlR, i.e. the RBS [http://parts.igem.org/Part:BBa_B0034 B0034] and a RBS optimized for ''rhlR'' using the [http://salis.psu.edu/software/RBSLibraryCalculatorSearchMode Salis Lab RBS Calculator]<sup>[[Team:ETH_Zurich/project/references#refSalis|[13]]]</sup>.<html><br><br></html> | #[http://parts.igem.org/Part:BBa_R0071:Experience Experience] - '''Promoter (RhlR & C4-HSL regulated), BBa_R0071:''' the amount of regulator [http://parts.igem.org/Part:BBa_C0171:Experience RhlR (C0171)] demonstrated an influence on the promoter's basal expression or leakiness as well as on the steepness of the response. This was investigated using two different RBSes controlling the expression level of RhlR, i.e. the RBS [http://parts.igem.org/Part:BBa_B0034 B0034] and a RBS optimized for ''rhlR'' using the [http://salis.psu.edu/software/RBSLibraryCalculatorSearchMode Salis Lab RBS Calculator]<sup>[[Team:ETH_Zurich/project/references#refSalis|[13]]]</sup>.<html><br><br></html> | ||
#[http://parts.igem.org/Part:BBa_C0062:Experience Experience] - '''Regulator LuxR, BBa_R0062:''' it could be shown, that the regulator LuxR not only activates the promoter [http://parts.igem.org/Part:BBa_R0062 BBa_R0062] together with 3CO6-HSL (the product of the enzyme [http://parts.igem.org/Part:BBa_C0161 LuxI (BBa_C0161)]), but also with 3CO12-HSL ([http://parts.igem.org/Part:BBa_C0178 LasI (BBa_C0178)] product) and C4-HSL ([http://parts.igem.org/Part:BBa_C0070 RhlI (BBa_C0070)] product).<html><br><br></html> | #[http://parts.igem.org/Part:BBa_C0062:Experience Experience] - '''Regulator LuxR, BBa_R0062:''' it could be shown, that the regulator LuxR not only activates the promoter [http://parts.igem.org/Part:BBa_R0062 BBa_R0062] together with 3CO6-HSL (the product of the enzyme [http://parts.igem.org/Part:BBa_C0161 LuxI (BBa_C0161)]), but also with 3CO12-HSL ([http://parts.igem.org/Part:BBa_C0178 LasI (BBa_C0178)] product) and C4-HSL ([http://parts.igem.org/Part:BBa_C0070 RhlI (BBa_C0070)] product).<html><br><br></html> | ||
| + | #[http://parts.igem.org/Part:BBa_C0179:Experience Experience] - '''Regulator LasR, BBa_C0179:''' it could be shown, that the regulator LasR mainly activates the promoter [http://parts.igem.org/Part:BBa_R0079 BBa_R0079] together with 3CO12-HSL (the product of the enzyme [http://parts.igem.org/Part:BBa_C0178 LasI (BBa_C0178)]) and only to a minor extend with 3CO6-HSL ([http://parts.igem.org/Part:BBa_C0161 LuxI (BBa_C0161)] product) and C4-HSL ([http://parts.igem.org/Part:BBa_C0070 RhlI (BBa_C0070)] product).<html><br><br></html> | ||
#BBa_C0161 | #BBa_C0161 | ||
<html></article></html> | <html></article></html> | ||
Revision as of 21:16, 11 October 2014
Data Page
Gene Circuit
For more information about the parts used in this circuit click on the parts in the picture that link to the registry.
Click here to see in action how the circuit responds to different inputs.
Our Favorite New Parts
- that
Used and Characterized Pre-Existing Parts
- Experience - Promoter (LuxR & 3OC6-HSL regulated), BBa_R0062: the promoter's EC50 could be shown to be dependent on the amount of regulator LuxR (BBa_C0062) in the system by using the three different constitutive promoters BBa_J23100, BBa_J23109, and BBa_J23111 for the production of LuxR. The amount of regulator also demonstrated an influence on the promoter's basal expression or leakiness. Additionally, it could be determined that the promoter can also be activated by the regulators LasR (BBa_C0179) and RhlR (C0171) thus showing crosstalk with these two quorum sensing systems.
- Experience - Promoter (LasR & 3OC12-HSL regulated), BBa_R0079: it could be shown that this promoter, which is usually activated by the regulator LasR (BBa_C0179) can also be activated by the regulators LuxR (BBa_C0062) and RhlR (C0171). Therefore, it shows crosstalk with these two quorum sensing systems.
- Experience - Promoter (RhlR & C4-HSL regulated), BBa_R0071: the amount of regulator RhlR (C0171) demonstrated an influence on the promoter's basal expression or leakiness as well as on the steepness of the response. This was investigated using two different RBSes controlling the expression level of RhlR, i.e. the RBS B0034 and a RBS optimized for rhlR using the Salis Lab RBS Calculator[13].
- Experience - Regulator LuxR, BBa_R0062: it could be shown, that the regulator LuxR not only activates the promoter BBa_R0062 together with 3CO6-HSL (the product of the enzyme LuxI (BBa_C0161)), but also with 3CO12-HSL (LasI (BBa_C0178) product) and C4-HSL (RhlI (BBa_C0070) product).
- Experience - Regulator LasR, BBa_C0179: it could be shown, that the regulator LasR mainly activates the promoter BBa_R0079 together with 3CO12-HSL (the product of the enzyme LasI (BBa_C0178)) and only to a minor extend with 3CO6-HSL (LuxI (BBa_C0161) product) and C4-HSL (RhlI (BBa_C0070) product).
- BBa_C0161
Characterized new parts
- there
