Team:ETH Zurich/data

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(Difference between revisions)
(Gene Circuit)
(Used and Characterized Pre-Existing Parts)
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==Used and Characterized Pre-Existing Parts==
==Used and Characterized Pre-Existing Parts==
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#[http://parts.igem.org/Part:BBa_R0062:Experience Experience] - '''Promoter (LuxR & 3OC6-HSL regulated), BBa_R0062:''' the promoter's EC<sub>50</sub> or K<sub>m</sub> could be shown to be dependent on the amount of regulator [http://parts.igem.org/Part:BBa_C0062:Experience LuxR (BBa_C0062)] in the system by using the three different constitutive promoters [http://parts.igem.org/Part:BBa_J23100:Experience BBa_J23100], [http://parts.igem.org/Part:BBa_J23109:Experience BBa_J23109], and [http://parts.igem.org/Part:BBa_J23111:Experience BBa_J23111] for the production of LuxR. The amount of regulator also showed an influence on the promoter's basal expression or leakiness. Additionally, it could be shown that the promoter can also be activated by the regulators [http://parts.igem.org/Part:BBa_C0179:Experience LasR (BBa_C0179)] and [http://parts.igem.org/Part:BBa_C0171:Experience RhlR (C0171)]. <html><br><br></html>
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#[http://parts.igem.org/Part:BBa_R0062:Experience Experience] - '''Promoter (LuxR & 3OC6-HSL regulated), BBa_R0062:''' the promoter's EC<sub>50</sub> could be shown to be dependent on the amount of regulator [http://parts.igem.org/Part:BBa_C0062:Experience LuxR (BBa_C0062)] in the system by using the three different constitutive promoters [http://parts.igem.org/Part:BBa_J23100:Experience BBa_J23100], [http://parts.igem.org/Part:BBa_J23109:Experience BBa_J23109], and [http://parts.igem.org/Part:BBa_J23111:Experience BBa_J23111] for the production of LuxR. The amount of regulator also showed an influence on the promoter's basal expression or leakiness. Additionally, it could be shown that the promoter can also be activated by the regulators [http://parts.igem.org/Part:BBa_C0179:Experience LasR (BBa_C0179)] and [http://parts.igem.org/Part:BBa_C0171:Experience RhlR (C0171)]. <html><br><br></html>
#[http://parts.igem.org/Part:BBa_R0079:Experience Experience] - '''Promoter (LasR & 3OC12-HSL regulated), BBa_R0079:''' the promoter's EC<sub>50</sub> or K<sub>m</sub> could be shown to be dependent on the amount of regulator [http://parts.igem.org/Part:BBa_C0062:Experience LuxR (BBa_C0062)] in the system. Additionally, it could be shown that the promoter can also be activated by the regulators [http://parts.igem.org/Part:BBa_C0179:Experience LasR (BBa_C0179)] and [http://parts.igem.org/Part:BBa_C0171:Experience RhlR (C0171)].
#[http://parts.igem.org/Part:BBa_R0079:Experience Experience] - '''Promoter (LasR & 3OC12-HSL regulated), BBa_R0079:''' the promoter's EC<sub>50</sub> or K<sub>m</sub> could be shown to be dependent on the amount of regulator [http://parts.igem.org/Part:BBa_C0062:Experience LuxR (BBa_C0062)] in the system. Additionally, it could be shown that the promoter can also be activated by the regulators [http://parts.igem.org/Part:BBa_C0179:Experience LasR (BBa_C0179)] and [http://parts.igem.org/Part:BBa_C0171:Experience RhlR (C0171)].
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Revision as of 19:38, 11 October 2014

iGEM ETH Zurich 2014