Team:ETH Zurich/data
From 2014.igem.org
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==Used and Characterized Pre-Existing Parts== | ==Used and Characterized Pre-Existing Parts== | ||
- | #[http://parts.igem.org/Part:BBa_R0062:Experience Experience] - '''Promoter (LuxR & 3OC6-HSL regulated), BBa_R0062:''' the promoter's EC<sub>50 | + | #[http://parts.igem.org/Part:BBa_R0062:Experience Experience] - '''Promoter (LuxR & 3OC6-HSL regulated), BBa_R0062:''' the promoter's EC<sub>50</sub> could be shown to be dependent on the amount of regulator [http://parts.igem.org/Part:BBa_C0062:Experience LuxR (BBa_C0062)] in the system by using the three different constitutive promoters [http://parts.igem.org/Part:BBa_J23100:Experience BBa_J23100], [http://parts.igem.org/Part:BBa_J23109:Experience BBa_J23109], and [http://parts.igem.org/Part:BBa_J23111:Experience BBa_J23111] for the production of LuxR. The amount of regulator also showed an influence on the promoter's basal expression or leakiness. Additionally, it could be shown that the promoter can also be activated by the regulators [http://parts.igem.org/Part:BBa_C0179:Experience LasR (BBa_C0179)] and [http://parts.igem.org/Part:BBa_C0171:Experience RhlR (C0171)]. <html><br><br></html> |
#[http://parts.igem.org/Part:BBa_R0079:Experience Experience] - '''Promoter (LasR & 3OC12-HSL regulated), BBa_R0079:''' the promoter's EC<sub>50</sub> or K<sub>m</sub> could be shown to be dependent on the amount of regulator [http://parts.igem.org/Part:BBa_C0062:Experience LuxR (BBa_C0062)] in the system. Additionally, it could be shown that the promoter can also be activated by the regulators [http://parts.igem.org/Part:BBa_C0179:Experience LasR (BBa_C0179)] and [http://parts.igem.org/Part:BBa_C0171:Experience RhlR (C0171)]. | #[http://parts.igem.org/Part:BBa_R0079:Experience Experience] - '''Promoter (LasR & 3OC12-HSL regulated), BBa_R0079:''' the promoter's EC<sub>50</sub> or K<sub>m</sub> could be shown to be dependent on the amount of regulator [http://parts.igem.org/Part:BBa_C0062:Experience LuxR (BBa_C0062)] in the system. Additionally, it could be shown that the promoter can also be activated by the regulators [http://parts.igem.org/Part:BBa_C0179:Experience LasR (BBa_C0179)] and [http://parts.igem.org/Part:BBa_C0171:Experience RhlR (C0171)]. | ||
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Revision as of 19:38, 11 October 2014
Data Page
Gene Circuit
For more information about the parts used in this circuit click on the parts in the picture that link to the registry.
Click here to see in action how the circuit responds to different inputs.
Our Favorite New Parts
- that
Used and Characterized Pre-Existing Parts
- Experience - Promoter (LuxR & 3OC6-HSL regulated), BBa_R0062: the promoter's EC50 could be shown to be dependent on the amount of regulator LuxR (BBa_C0062) in the system by using the three different constitutive promoters BBa_J23100, BBa_J23109, and BBa_J23111 for the production of LuxR. The amount of regulator also showed an influence on the promoter's basal expression or leakiness. Additionally, it could be shown that the promoter can also be activated by the regulators LasR (BBa_C0179) and RhlR (C0171).
- Experience - Promoter (LasR & 3OC12-HSL regulated), BBa_R0079: the promoter's EC50 or Km could be shown to be dependent on the amount of regulator LuxR (BBa_C0062) in the system. Additionally, it could be shown that the promoter can also be activated by the regulators LasR (BBa_C0179) and RhlR (C0171).
Characterized new parts
- there