Gene Circuit and Parts
Hover the parts and it will lead you to the registry.For more information about the parts used in this circuit click on the parts in the picture that link to the registry.
(→Used and Characterized Pre-Existing Parts) |
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==Our Favorite New Parts== | ==Our Favorite New Parts== | ||
- | #[http://parts.igem.org/Part:BBa_K1541000 Main Page] - '''Riboregulated Promoter (LuxR/3OC6-HSL, RR12y), BBa_K1541000:''' this riboregulated promoter construct contains the quorum sensing promoter [http://parts.igem.org/Part:BBa_R0062 BBa_R0062] which can be activated in presence of [http://parts.igem.org/Part:BBa_C0062:Experience LuxR (BBa_C0062)] and 3OC6-HSL | + | #[http://parts.igem.org/Part:BBa_K1541000 Main Page] - '''Riboregulated Promoter (LuxR/3OC6-HSL, RR12y), BBa_K1541000:''' this riboregulated promoter construct contains the quorum sensing promoter [http://parts.igem.org/Part:BBa_R0062 BBa_R0062] which can be activated in presence of [http://parts.igem.org/Part:BBa_C0062:Experience LuxR (BBa_C0062)] and 3OC6-HSL, which is the product of the enzyme [http://parts.igem.org/Part:BBa_C0161 LuxI (BBa_C0161)]. However, this promoter by itself shows high leakiness. Together with the ribogegulator 12y<sup>[[Team:ETH_Zurich/project/references#refCallura|[18]]]</sup> the leakiness could be reduced by almost two orders of magnitude and the ON/OFF ratio increased approximately 6 times.<html><br><br></html> |
#[http://parts.igem.org/Part:BBa_K1541006 Main Page] - '''sfGFP under Riboregulated Promoter (LuxR/3OC6-HSL, RR12y), BBa_K1541006:''' this part contains the same riboregulated promoter system as the part [http://parts.igem.org/Part:BBa_K1541000 BBa_K1541000] succeeded by the gene for sfGFP. This construct was used to characterize the properties of the riboregulated quorum sensing promoter [http://parts.igem.org/Part:BBa_K1541000 BBa_K1541000] and can be used in a 3OC6-HSL sensor where low leakiness is desirable. | #[http://parts.igem.org/Part:BBa_K1541006 Main Page] - '''sfGFP under Riboregulated Promoter (LuxR/3OC6-HSL, RR12y), BBa_K1541006:''' this part contains the same riboregulated promoter system as the part [http://parts.igem.org/Part:BBa_K1541000 BBa_K1541000] succeeded by the gene for sfGFP. This construct was used to characterize the properties of the riboregulated quorum sensing promoter [http://parts.igem.org/Part:BBa_K1541000 BBa_K1541000] and can be used in a 3OC6-HSL sensor where low leakiness is desirable. | ||
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Gene Circuit and Parts
Hover the parts and it will lead you to the registry.
For more information about the parts used in this circuit click on the parts in the picture that link to the registry.
Click here to see in action how the circuit responds to different inputs.