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| - | <h2>9/4/14</h2>
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| - | <p>Objective: test backbone shuffled antitracr bacteria</p>
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| - | <ul><li>Started colonies from plates and diluted in to +/- aTc this morning. Let shake for 5(?) hours before collecting 500uL and spin/resusp in PBS for flow.</li><li>Took data to flowjo and plotted histograms below. This is ungated fluorescence</li><li></li><li><span style="overflow: hidden; display: inline-block; margin: 0.00px 0.00px; border: 0.00px solid #000000; transform: rotate(0.00rad) translateZ(0px); -webkit-transform: rotate(0.00rad) translateZ(0px); width: 624.00px; height: 482.67px;"><img alt="" src="images/image44.jpg" style="width: 624.00px; height: 482.67px; margin-left: 0.00px; margin-top: 0.00px; transform: rotate(0.00rad) translateZ(0px); -webkit-transform: rotate(0.00rad) translateZ(0px);" title=""></li><li>table with mean GFPAs</li></ul><a href="#" name="096a26566c8f532b5e11f6f110d57e6edec006da"></a><a href="#" name="3"></a><table cellpadding="0" cellspacing="0"><tbody><tr><td><p></p></td><td><p></p></td><td><p></p></td><td><p>ungated GFPA</p></td><td><p>+/- aTc</p></td></tr><tr><td><p>1: if142014-09-04.001.fcs</p></td><td><p>AC-1</p></td><td><p>+ aTc</p></td><td><p>0.402</p></td><td><p>1.185840708</p></td></tr><tr><td><p>2: if142014-09-04.002.fcs</p></td><td><p>AC-2</p></td><td><p>+ aTc</p></td><td><p>0.455</p></td><td><p>1.197368421</p></td></tr><tr><td><p>3: if142014-09-04.003.fcs</p></td><td><p>G-1</p></td><td><p>+ aTc</p></td><td><p>14.9</p></td><td><p>0.949044586</p></td></tr><tr><td><p>4: if142014-09-04.004.fcs</p></td><td><p>G-2</p></td><td><p>+ aTc</p></td><td><p>11.7</p></td><td><p>0.573529412</p></td></tr><tr><td><p>5: if142014-09-04.005.fcs</p></td><td><p>G-3</p></td><td><p>+ aTc</p></td><td><p>8.91</p></td><td><p>0.530357143</p></td></tr><tr><td><p>6: if142014-09-04.006.fcs</p></td><td><p>G-4</p></td><td><p>+ aTc</p></td><td><p>5.92</p></td><td><p>0.34619883</p></td></tr><tr><td><p>7: if142014-09-04.007.fcs</p></td><td><p>C-1</p></td><td><p>+ aTc</p></td><td><p>186</p></td><td><p>1.016393443</p></td></tr><tr><td><p>8: if142014-09-04.008.fcs</p></td><td><p>C-2</p></td><td><p>+ aTc</p></td><td><p>224</p></td><td><p>1.230769231</p></td></tr><tr><td><p>9: if142014-09-04.009.fcs</p></td><td><p>C-3</p></td><td><p>+ aTc</p></td><td><p>214</p></td><td><p>0.922413793</p></td></tr><tr><td><p>10: if142014-09-04.010.fcs</p></td><td><p>C-4</p></td><td><p>+ aTc</p></td><td><p>222</p></td><td><p>1.193548387</p></td></tr><tr><td><p>11: if142014-09-04.011.fcs</p></td><td><p>AC-1</p></td><td><p>- aTc</p></td><td><p>0.339</p></td><td><p></p></td></tr><tr><td><p>12: if142014-09-04.012.fcs</p></td><td><p>AC-2</p></td><td><p>- aTc</p></td><td><p>0.38</p></td><td><p></p></td></tr><tr><td><p>13: if142014-09-04.013.fcs</p></td><td><p>G-1</p></td><td><p>- aTc</p></td><td><p>15.7</p></td><td><p></p></td></tr><tr><td><p>14: if142014-09-04.014.fcs</p></td><td><p>G-2</p></td><td><p>- aTc</p></td><td><p>20.4</p></td><td><p></p></td></tr><tr><td><p>15: if142014-09-04.015.fcs</p></td><td><p>G-3</p></td><td><p>- aTc</p></td><td><p>16.8</p></td><td><p></p></td></tr><tr><td><p>16: if142014-09-04.016.fcs</p></td><td><p>G-4</p></td><td><p>- aTc</p></td><td><p>17.1</p></td><td><p></p></td></tr><tr><td><p>17: if142014-09-04.017.fcs</p></td><td><p>C-1</p></td><td><p>- aTc</p></td><td><p>183</p></td><td><p></p></td></tr><tr><td><p>18: if142014-09-04.018.fcs</p></td><td><p>C-2</p></td><td><p>- aTc</p></td><td><p>182</p></td><td><p></p></td></tr><tr><td><p>19: if142014-09-04.019.fcs</p></td><td><p>C-3</p></td><td><p>- aTc</p></td><td><p>232</p></td><td><p></p></td></tr><tr><td><p>20: if142014-09-04.020.fcs</p></td><td><p>C-4</p></td><td><p>- aTc</p></td><td><p>186</p></td><td><p></p></td></tr></tbody></table><ul><li>aTc treatment seems to be moving fluor in the opposite direction of what we’d expect of antitracr was doing its job. The degree is inconsistent, reflow is called for. </li></ul><p></p><p></p><p></p><p></p><hr style="page-break-before:always;display:none;"><p></p><hr style="page-break-before:always;display:none;"><p></p><p></p><h2><a name="h.ohhk3mdbkmcm"></a>9/8/14</h2><p>Objective: retest backbone shuffled antitracr bacteria</p><ul><li>Yesterday picked 4 new colonies from plates and 4 from 9/4/14 frozen stocks and grew in LB + antibiotics overnight. Diluted in to +/- aTc this morning and let shake for 5(?) hours before collecting 500uL and spin/resusp in PBS</li><li>G1-4 are old frozen coli with K608012, pdCas9-GFP1, and 1A2-R0040-antitracr, C1-4 are old frozen with pdCas9. clones 5-8 are new from plates. AC are no-fluor (1A2 and pdCas9) but they were diluted in to triple antibiotic medium instead of double so they did not grow and are not reliable indicators of bg fluorescence on this run.</li><li>After flow, isolated singlets with flowjo (smalls) or left alone (bugs) and got gfpa unmodified or normalized by fsca or ssca. </li><li></li></ul><a href="#" name="777f62c089f7173b14ec6d88236db6c3e53afff8"></a><a href="#" name="4"></a><table cellpadding="0" cellspacing="0"<><tbody><tr><td><p></p></td><td><p></p></td><td><p></p></td><td><p></p></td><td><p>smalls</p></td><td><p></p></td><td><p></p></td><td><p>bugs</p></td><td><p></p></td></tr><tr><td><p></p></td><td><p></p></td><td><p>aTc</p></td><td><p>gfpa</p></td><td><p>gfpa/fsca*1000</p></td><td><p>gfpa/ssca*1000</p></td><td><p>gfpa</p></td><td><p>gfpa/fsca*1000</p></td><td><p>gfpa/ssca*1000</p></td></tr><tr><td><p>1: if142014-09-08.001.fcs</p></td><td><p>G1</p></td><td><p>+</p></td><td><p>5.25</p></td><td><p>243</p></td><td><p>112</p></td><td><p>7.79</p></td><td><p>247</p></td><td><p>114</p></td></tr><tr><td><p>2: if142014-09-08.002.fcs</p></td><td><p>G2</p></td><td><p>+</p></td><td><p>4.41</p></td><td><p>226</p></td><td><p>78.1</p></td><td><p>7.12</p></td><td><p>228</p></td><td><p>76.2</p></td></tr><tr><td><p>3: if142014-09-08.003.fcs</p></td><td><p>G3</p></td><td><p>+</p></td><td><p>4.66</p></td><td><p>231</p></td><td><p>84.8</p></td><td><p>7.8</p></td><td><p>231</p></td><td><p>83.4</p></td></tr><tr><td><p>4: if142014-09-08.004.fcs</p></td><td><p>G4</p></td><td><p>+</p></td><td><p>3.86</p></td><td><p>191</p></td><td><p>70.1</p></td><td><p>5.99</p></td><td><p>192</p></td><td><p>67.9</p></td></tr><tr><td><p>5: if142014-09-08.005.fcs</p></td><td><p>G1</p></td><td><p>-</p></td><td><p>6.81</p></td><td><p>311</p></td><td><p>145</p></td><td><p>8.56</p></td><td><p>310</p></td><td><p>143</p></td></tr><tr><td><p>6: if142014-09-08.006.fcs</p></td><td><p>G2</p></td><td><p>-</p></td><td><p>6.37</p></td><td><p>337</p></td><td><p>153</p></td><td><p>7.25</p></td><td><p>323</p></td><td><p>145</p></td></tr><tr><td><p>7: if142014-09-08.007.fcs</p></td><td><p>G3</p></td><td><p>-</p></td><td><p>6.5</p></td><td><p>318</p></td><td><p>143</p></td><td><p>8.24</p></td><td><p>305</p></td><td><p>136</p></td></tr><tr><td><p>8: if142014-09-08.008.fcs</p></td><td><p>G4</p></td><td><p>-</p></td><td><p>6.14</p></td><td><p>298</p></td><td><p>140</p></td><td><p>7.66</p></td><td><p>288</p></td><td><p>131</p></td></tr><tr><td><p>9: if142014-09-08.009.fcs</p></td><td><p>C1</p></td><td><p>+</p></td><td><p>88.9</p></td><td><p>4071</p></td><td><p>1777</p></td><td><p>117</p></td><td><p>3978</p></td><td><p>1706</p></td></tr><tr><td><p>10: if142014-09-08.010.fcs</p></td><td><p>C2</p></td><td><p>+</p></td><td><p>83.5</p></td><td><p>4073</p></td><td><p>1547</p></td><td><p>120</p></td><td><p>3969</p></td><td><p>1422</p></td></tr><tr><td><p>11: if142014-09-08.011.fcs</p></td><td><p>C3</p></td><td><p>+</p></td><td><p>81.7</p></td><td><p>3957</p></td><td><p>1551</p></td><td><p>115</p></td><td><p>3840</p></td><td><p>1437</p></td></tr><tr><td><p>12: if142014-09-08.012.fcs</p></td><td><p>C4</p></td><td><p>+</p></td><td><p>77.2</p></td><td><p>3915</p></td><td><p>1577</p></td><td><p>95.7</p></td><td><p>3883</p></td><td><p>1503</p></td></tr><tr><td><p>13: if142014-09-08.013.fcs</p></td><td><p>C1</p></td><td><p>-</p></td><td><p>77.7</p></td><td><p>3686</p></td><td><p>1471</p></td><td><p>100</p></td><td><p>3545</p></td><td><p>1411</p></td></tr><tr><td><p>14: if142014-09-08.014.fcs</p></td><td><p>C2</p></td><td><p>-</p></td><td><p>64.5</p></td><td><p>3093</p></td><td><p>1445</p></td><td><p>88.3</p></td><td><p>2962</p></td><td><p>1373</p></td></tr><tr><td><p>15: if142014-09-08.015.fcs</p></td><td><p>C3</p></td><td><p>-</p></td><td><p>80.9</p></td><td><p>3971</p></td><td><p>1541</p></td><td><p>106</p></td><td><p>3771</p></td><td><p>1432</p></td></tr><tr><td><p>16: if142014-09-08.016.fcs</p></td><td><p>C4</p></td><td><p>-</p></td><td><p>58.9</p></td><td><p>2899</p></td><td><p>1299</p></td><td><p>79.7</p></td><td><p>2727</p></td><td><p>1220</p></td></tr><tr><td><p>17: if142014-09-08.017.fcs</p></td><td><p>G5</p></td><td><p>+</p></td><td><p>4.1</p></td><td><p>210</p></td><td><p>79.1</p></td><td><p>5.4</p></td><td><p>209</p></td><td><p>76.2</p></td></tr><tr><td><p>18: if142014-09-08.018.fcs</p></td><td><p>G6</p></td><td><p>+</p></td><td><p>4.9</p></td><td><p>225</p></td><td><p>98.2</p></td><td><p>7.07</p></td><td><p>226</p></td><td><p>97.6</p></td></tr><tr><td><p>19: if142014-09-08.019.fcs</p></td><td><p>G7</p></td><td><p>+</p></td><td><p>4.42</p></td><td><p>220</p></td><td><p>84.7</p></td><td><p>5.97</p></td><td><p>218</p></td><td><p>81.7</p></td></tr><tr><td><p>20: if142014-09-08.020.fcs</p></td><td><p>G8</p></td><td><p>+</p></td><td><p>4.19</p></td><td><p>211</p></td><td><p>81.3</p></td><td><p>5.68</p></td><td><p>213</p></td><td><p>79.5</p></td></tr><tr><td><p>21: if142014-09-08.021.fcs</p></td><td><p>G5</p></td><td><p>-</p></td><td><p>9.73</p></td><td><p>477</p></td><td><p>201</p></td><td><p>12.5</p></td><td><p>455</p></td><td><p>190</p></td></tr><tr><td><p>22: if142014-09-08.022.fcs</p></td><td><p>G6</p></td><td><p>-</p></td><td><p>9.68</p></td><td><p>450</p></td><td><p>197</p></td><td><p>12.9</p></td><td><p>449</p></td><td><p>196</p></td></tr><tr><td><p>23: if142014-09-08.023.fcs</p></td><td><p>G7</p></td><td><p>-</p></td><td><p>10.1</p></td><td><p>482</p></td><td><p>212</p></td><td><p>13.3</p></td><td><p>464</p></td><td><p>198</p></td></tr><tr><td><p>24: if142014-09-08.024.fcs</p></td><td><p>G8</p></td><td><p>-</p></td><td><p>10.1</p></td><td><p>489</p></td><td><p>216</p></td><td><p>13.3</p></td><td><p>473</p></td><td><p>204</p></td></tr><tr><td><p>25: if142014-09-08.025.fcs</p></td><td><p>C5</p></td><td><p>+</p></td><td><p>79.6</p></td><td><p>3958</p></td><td><p>1486</p></td><td><p>113</p></td><td><p>3813</p></td><td><p>1374</p></td></tr><tr><td><p>26: if142014-09-08.026.fcs</p></td><td><p>C6</p></td><td><p>+</p></td><td><p>80.8</p></td><td><p>4045</p></td><td><p>1514</p></td><td><p>113</p></td><td><p>4043</p></td><td><p>1425</p></td></tr><tr><td><p>27: if142014-09-08.027.fcs</p></td><td><p>C7</p></td><td><p>+</p></td><td><p>80.7</p></td><td><p>4053</p></td><td><p>1491</p></td><td><p>116</p></td><td><p>3921</p></td><td><p>1375</p></td></tr><tr><td><p>28: if142014-09-08.028.fcs</p></td><td><p>C8</p></td><td><p>+</p></td><td><p>79.8</p></td><td><p>3804</p></td><td><p>1555</p></td><td><p>103</p></td><td><p>3634</p></td><td><p>1508</p></td></tr><tr><td><p>29: if142014-09-08.029.fcs</p></td><td><p>C5</p></td><td><p>-</p></td><td><p>68.9</p></td><td><p>3268</p></td><td><p>1505</p></td><td><p>95.7</p></td><td><p>3128</p></td><td><p>1419</p></td></tr><tr><td><p>30: if142014-09-08.030.fcs</p></td><td><p>C6</p></td><td><p>-</p></td><td><p>80.2</p></td><td><p>3806</p></td><td><p>1639</p></td><td><p>110</p></td><td><p>3630</p></td><td><p>1541</p></td></tr><tr><td><p>31: if142014-09-08.031.fcs</p></td><td><p>C7</p></td><td><p>-</p></td><td><p>67.8</p></td><td><p>3240</p></td><td><p>1498</p></td><td><p>91.7</p></td><td><p>3041</p></td><td><p>1412</p></td></tr><tr><td><p>32: if142014-09-08.032.fcs</p></td><td><p>C8</p></td><td><p>-</p></td><td><p>83.7</p></td><td><p>3810</p></td><td><p>1675</p></td><td><p>107</p></td><td><p>3618</p></td><td><p>1621</p></td></tr><tr><td><p>33: if142014-09-08.033.fcs</p></td><td><p>AC1</p></td><td><p>+</p></td><td><p>0.895</p></td><td><p>40.2</p></td><td><p>16.8</p></td><td><p>0.854</p></td><td><p>34.9</p></td><td><p>12.9</p></td></tr><tr><td><p>34: if142014-09-08.034.fcs</p></td><td><p>AC3</p></td><td><p>+</p></td><td><p>0.539</p></td><td><p>28.4</p></td><td><p>11</p></td><td><p>0.785</p></td><td><p>27.4</p></td><td><p>10.7</p></td></tr><tr><td><p>35: if142014-09-08.035.fcs</p></td><td><p>AC1</p></td><td><p>-</p></td><td><p>0.464</p></td><td><p>25.8</p></td><td><p>9.39</p></td><td><p>0.605</p></td><td><p>25.9</p></td><td><p>8.65</p></td></tr><tr><td><p>36: if142014-09-08.036.fcs</p></td><td><p>AC3</p></td><td><p>-</p></td><td><p>0.532</p></td><td><p>28.3</p></td><td><p>10.4</p></td><td><p>0.661</p></td><td><p>26.2</p></td><td><p>9.23</p></td></tr></tbody></table><p></p><ul><li>Got ratios of fluorescence in +/- aTc and am reporting them in the table below</li></ul><a href="#" name="803fb1152dc946dc3b98f720d1637f36481109e8"></a><a href="#" name="5"></a><table cellpadding="0" cellspacing="0"><tbody><tr><td><p>n/a</p></td><td><p>n/a</p></td><td><p>+/-</p></td><td><p>smalls</p></td><td><p></p></td><td><p></p></td><td><p>bugs</p></td><td><p></p></td></tr><tr><td><p>n/a</p></td><td><p>n/a</p></td><td><p>gfpa</p></td><td><p>gfpa/fsca*1000</p></td><td><p>gfpa/ssca*1000</p></td><td><p>gfpa</p></td><td><p>gfpa/fsca*1000</p></td><td><p>gfpa/ssca*1000</p></td></tr><tr><td><p>1: if142014-09-08.001.fcs</p></td><td><p>G1</p></td><td><p>0.771</p></td><td><p>0.781</p></td><td><p>0.772</p></td><td><p>0.910</p></td><td><p>0.797</p></td><td><p>0.797</p></td></tr><tr><td><p>2: if142014-09-08.002.fcs</p></td><td><p>G2</p></td><td><p>0.692</p></td><td><p>0.671</p></td><td><p>0.510</p></td><td><p>0.982</p></td><td><p>0.706</p></td><td><p>0.526</p></td></tr><tr><td><p>3: if142014-09-08.003.fcs</p></td><td><p>G3</p></td><td><p>0.717</p></td><td><p>0.726</p></td><td><p>0.593</p></td><td><p>0.947</p></td><td><p>0.757</p></td><td><p>0.613</p></td></tr><tr><td><p>4: if142014-09-08.004.fcs</p></td><td><p>G4</p></td><td><p>0.629</p></td><td><p>0.641</p></td><td><p>0.501</p></td><td><p>0.782</p></td><td><p>0.667</p></td><td><p>0.518</p></td></tr><tr><td><p>9: if142014-09-08.009.fcs</p></td><td><p>C1</p></td><td><p>1.144</p></td><td><p>1.104</p></td><td><p>1.208</p></td><td><p>1.170</p></td><td><p>1.122</p></td><td><p>1.209</p></td></tr><tr><td><p>10: if142014-09-08.010.fcs</p></td><td><p>C2</p></td><td><p>1.295</p></td><td><p>1.317</p></td><td><p>1.071</p></td><td><p>1.359</p></td><td><p>1.340</p></td><td><p>1.036</p></td></tr><tr><td><p>11: if142014-09-08.011.fcs</p></td><td><p>C3</p></td><td><p>1.010</p></td><td><p>0.996</p></td><td><p>1.006</p></td><td><p>1.085</p></td><td><p>1.018</p></td><td><p>1.003</p></td></tr><tr><td><p>12: if142014-09-08.012.fcs</p></td><td><p>C4</p></td><td><p>1.311</p></td><td><p>1.350</p></td><td><p>1.214</p></td><td><p>1.201</p></td><td><p>1.424</p></td><td><p>1.232</p></td></tr><tr><td><p>17: if142014-09-08.017.fcs</p></td><td><p>G5</p></td><td><p>0.421</p></td><td><p>0.440</p></td><td><p>0.394</p></td><td><p>0.432</p></td><td><p>0.459</p></td><td><p>0.401</p></td></tr><tr><td><p>18: if142014-09-08.018.fcs</p></td><td><p>G6</p></td><td><p>0.506</p></td><td><p>0.500</p></td><td><p>0.498</p></td><td><p>0.548</p></td><td><p>0.503</p></td><td><p>0.498</p></td></tr><tr><td><p>19: if142014-09-08.019.fcs</p></td><td><p>G7</p></td><td><p>0.438</p></td><td><p>0.456</p></td><td><p>0.400</p></td><td><p>0.449</p></td><td><p>0.470</p></td><td><p>0.413</p></td></tr><tr><td><p>20: if142014-09-08.020.fcs</p></td><td><p>G8</p></td><td><p>0.415</p></td><td><p>0.431</p></td><td><p>0.376</p></td><td><p>0.427</p></td><td><p>0.450</p></td><td><p>0.390</p></td></tr><tr><td><p>25: if142014-09-08.025.fcs</p></td><td><p>C5</p></td><td><p>1.155</p></td><td><p>1.211</p></td><td><p>0.987</p></td><td><p>1.181</p></td><td><p>1.219</p></td><td><p>0.968</p></td></tr><tr><td><p>26: if142014-09-08.026.fcs</p></td><td><p>C6</p></td><td><p>1.007</p></td><td><p>1.063</p></td><td><p>0.924</p></td><td><p>1.027</p></td><td><p>1.114</p></td><td><p>0.925</p></td></tr><tr><td><p>27: if142014-09-08.027.fcs</p></td><td><p>C7</p></td><td><p>1.190</p></td><td><p>1.251</p></td><td><p>0.995</p></td><td><p>1.265</p></td><td><p>1.289</p></td><td><p>0.974</p></td></tr><tr><td><p>28: if142014-09-08.028.fcs</p></td><td><p>C8</p></td><td><p>0.953</p></td><td><p>0.998</p></td><td><p>0.928</p></td><td><p>0.963</p></td><td><p>1.004</p></td><td><p>0.930</p></td></tr><tr><td><p>33: if142014-09-08.033.fcs</p></td><td><p>AC1</p></td><td><p>1.929</p></td><td><p>1.558</p></td><td><p>1.789</p></td><td><p>1.412</p></td><td><p>1.347</p></td><td><p>1.491</p></td></tr><tr><td><p>34: if142014-09-08.034.fcs</p></td><td><p>AC3</p></td><td><p>1.013</p></td><td><p>1.004</p></td><td><p>1.058</p></td><td><p>1.188</p></td><td><p>1.046</p></td><td><p>1.159</p></td></tr></tbody></table><ul><li></li></ul><hr style="page-break-before:always;display:none;"><p><span style="overflow: hidden; display: inline-block; margin: 0.00px 0.00px; border: 0.00px solid #000000; transform: rotate(0.00rad) translateZ(0px); -webkit-transform: rotate(0.00rad) translateZ(0px); width: 560.00px; height: 420.00px;"><img alt="" src="images/image19.jpg" style="width: 560.00px; height: 420.00px; margin-left: 0.00px; margin-top: 0.00px; transform: rotate(0.00rad) translateZ(0px); -webkit-transform: rotate(0.00rad) translateZ(0px);" title=""></p><p>Box plot of just G1-4 and C1-4. Note that the G clones from plates (clones 5-8) have even smaller ratios. </p><hr style="page-break-before:always;display:none;"><p></p><p></p><p></p><p></p><hr style="page-break-before:always;display:none;"><p></p><p></p><p></p><p></p><h2><a name="h.h7pf932a50dt"></a>9/9/14</h2><p>Objective: assembly decoy binding site arrays</p><ul><li>Garima sets up a PCR and is asked to fill in notes about it.</li><li>Results</li><li>Charlie sets up a new set of tubes for PCR with a 5 rx master mix containing</li></ul><ul><li>190uL H2O</li><li>50 uL Q5 buffer</li><li>2.0 uL oligo 1 (gfp1-spacer-gfp1)</li><li>2.5 uL Q5 poly</li><li>5 uL dNTPs</li></ul><ul><li>And then divides that in to four tubes before adding in the following volumes of oligo 2 (spacer-gfp1-space)</li></ul><a href="#" name="fd71260aa00de3f043dfc426450b7d96e13e45b0"></a><a href="#" name="6"></a><table cellpadding="0" cellspacing="0"><tbody><tr><td><p>tube number</p></td><td><p>1</p></td><td><p>2</p></td><td><p>3</p></td><td><p>4</p></td></tr><tr><td><p>uL oligo 2</p></td><td><p>0</p></td><td><p>0.5</p></td><td><p>1.0</p></td><td><p>2.0</p></td></tr></tbody></table><ul><li><span style="overflow: hidden; display: inline-block; margin: 0.00px 0.00px; border: 0.00px solid #000000; transform: rotate(0.00rad) translateZ(0px); -webkit-transform: rotate(0.00rad) translateZ(0px); width: 624.00px; height: 468.00px;"><img alt="Sep 10, 2014 1:24:31 PM.jpg" src="images/image04.jpg" style="width: 624.00px; height: 468.00px; margin-left: 0.00px; margin-top: 0.00px; transform: rotate(0.00rad) translateZ(0px); -webkit-transform: rotate(0.00rad) translateZ(0px);" title=""></li></ul><ul><li>Streakiness on the row with 0.5uL of oligo 2 looks promising? IGEM protocol on the thermal cycler modified</li></ul><h2><a name="h.3838tuwxw3ds"></a>
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Latest revision as of 05:25, 17 October 2014
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