Team:Duke/Notebook

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Lab Notebook

July 2014
Month 4 of Project
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August 2014
Month 5 of Project
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September 2014
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October 2014
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April Overview

Click here for the April Lab Notebook

The objective in April, while classes were still in session, was to make something called the Z4EV-dCas9-MXI1 construct. The model organism used was yeast, and this was a continuation of last year’s project.

The general idea was to fuse a repressor domain onto the dCas9 gene, to make an even stronger repressor. MXI1 is the repressor domain, and Z4EV is the promoter that was used. It’s originally a zinc-finger induced promoter for yeast.

This approach was abandoned because the general consensus was that repression is harder to achieve in yeast. Instead of trying to continue last year’s project, we decided to start afresh, with a new model organism, E. coli.

May, June Overview

Objectives

This is what we were trying to accomplish in May and June. May and June Lab Notebook

July Overview

Objectives

This is what we were trying to accomplish in July. July Notebook

August Overview

Objectives

This is what we were trying to accomplish in August. August Lab Notebook

September Overview

Objectives

This is what we were trying to accomplish in September. September Lab Notebook

October Overview

Objectives

This is what we were trying to accomplish in October. October Lab Notebook

Protocols

Protocols can be found here