|
|
| (29 intermediate revisions not shown) |
| Line 2: |
Line 2: |
| | {{Template:Team:DukeMenu/CSS}} | | {{Template:Team:DukeMenu/CSS}} |
| | {{Template:Team:DukeNB/CSS}} | | {{Template:Team:DukeNB/CSS}} |
| - |
| |
| | | | |
| | <html> | | <html> |
| Line 10: |
Line 9: |
| | | | |
| | <body> | | <body> |
| - | <p class="big"> Lab Notebook </p>
| + | <div class=" month"><a href="https://2014.igem.org/Team:Duke/Notebook/Overview">Overview of Each Month </a></div> |
| - | <div id="sticky_cal_wrap">
| + | <div class=" month"><a href="https://2014.igem.org/Team:Duke/Notebook/ Months">Notebook Entries by Month</a></div> |
| - | <div id="cal">
| + | <div class=" month"><a href="https://2014.igem.org/Team:Duke/Notebook/Protocols">Protocols</a></div> |
| - | | + | |
| - | | + | |
| - | | + | |
| - | | + | |
| - | | + | |
| - | <table>
| + | |
| - | <tr>
| + | |
| - | <td colspan="7"> July 2014 </td>
| + | |
| - | </tr>
| + | |
| - | <tr>
| + | |
| - | <td colspan="7" align=center><i> Month 4 of Project </i></td>
| + | |
| - | </tr>
| + | |
| - | <tr>
| + | |
| - | <td>sun</td>
| + | |
| - | <td>mon</td>
| + | |
| - | <td>tue</td>
| + | |
| - | <td>wed</td>
| + | |
| - | <td>thu</td>
| + | |
| - | <td>fri</td>
| + | |
| - | <td>sat</td>
| + | |
| - | </tr>
| + | |
| - | <tr>
| + | |
| - | <td></td>
| + | |
| - | <td></td>
| + | |
| - | <td><a href="https://2014.igem.org/Team:Duke/Notebook#jul1">1</a></td>
| + | |
| - | <td><a href="https://2014.igem.org/Team:Duke/Notebook#jul2">2</a></td>
| + | |
| - | <td><a href="https://2014.igem.org/Team:Duke/Notebook#jul3">3</a></td>
| + | |
| - | <td><a href="https://2014.igem.org/Team:Duke/Notebook#jul4">4</a></td>
| + | |
| - | <td ><a href="https://2014.igem.org/Team:Duke/Notebook#jul5">5</a></td>
| + | |
| - | </tr>
| + | |
| - | <tr>
| + | |
| - | <td><a href="https://2014.igem.org/Team:Duke/Notebook#jul6">6</a></td>
| + | |
| - | <td><a href="https://2014.igem.org/Team:Duke/Notebook#jul7">7</a></td>
| + | |
| - | <td><a href="https://2014.igem.org/Team:Duke/Notebook#jul8">8</a></td>
| + | |
| - | <td><a href="https://2014.igem.org/Team:Duke/Notebook#jul9">9</a></td>
| + | |
| - | <td><a href="https://2014.igem.org/Team:Duke/Notebook#jul10">10</a></td>
| + | |
| - | <td><a href="https://2014.igem.org/Team:Duke/Notebook#jul11">11</a></td>
| + | |
| - | <td>12</td>
| + | |
| - | </tr>
| + | |
| - | <tr>
| + | |
| - | <td>13</td>
| + | |
| - | <td><a href="https://2014.igem.org/Team:Duke/Notebook#jul14">14</a></td>
| + | |
| - | <td><a href="https://2014.igem.org/Team:Duke/Notebook#jul15">15</a></td>
| + | |
| - | <td><a href="https://2014.igem.org/Team:Duke/Notebook#jul16">16</a></td>
| + | |
| - | <td><a href="https://2014.igem.org/Team:Duke/Notebook#jul17">17</a></td>
| + | |
| - | <td><a href="https://2014.igem.org/Team:Duke/Notebook#jul18">18</a></td>
| + | |
| - | <td>19</td>
| + | |
| - | </tr>
| + | |
| - | <tr>
| + | |
| - | <td><a href="https://2014.igem.org/Team:Duke/Notebook#jul20">20</a></td>
| + | |
| - | <td><a href="https://2014.igem.org/Team:Duke/Notebook#jul21">21</a></td>
| + | |
| - | <td>22</td>
| + | |
| - | <td>23</td>
| + | |
| - | <td>24</td>
| + | |
| - | <td>25</td>
| + | |
| - | <td>26</td>
| + | |
| - | </tr>
| + | |
| - | <tr>
| + | |
| - | <td>27</td>
| + | |
| - | <td>28</td>
| + | |
| - | <td>29</td>
| + | |
| - | <td>30</td>
| + | |
| - | <td>31</td>
| + | |
| - | <td></td>
| + | |
| - | <td></td>
| + | |
| - | </tr>
| + | |
| - | <tr>
| + | |
| - | <td></td>
| + | |
| - | <td></td>
| + | |
| - | <td></td>
| + | |
| - | <td></td>
| + | |
| - | <td></td>
| + | |
| - | <td></td>
| + | |
| - | <td></td>
| + | |
| - | </tr>
| + | |
| - | </table>
| + | |
| - | | + | |
| - | <table>
| + | |
| - | <tr>
| + | |
| - | <td colspan="7"> August 2014 </td>
| + | |
| - | </tr>
| + | |
| - | <tr>
| + | |
| - | <td colspan="7" align=center><i> Month 5 of Project </i></td>
| + | |
| - | </tr>
| + | |
| - | <tr>
| + | |
| - | <td align=center>sun</td>
| + | |
| - | <td align=center>mon</td>
| + | |
| - | <td align=center>tue</td>
| + | |
| - | <td align=center>wed</td>
| + | |
| - | <td align=center>thu</td>
| + | |
| - | <td align=center>fri</td>
| + | |
| - | <td align=center>sat</td>
| + | |
| - | </tr>
| + | |
| - | <tr>
| + | |
| - | <td align=center></td>
| + | |
| - | <td align=center></td>
| + | |
| - | <td align=center></td>
| + | |
| - | <td align=center></td>
| + | |
| - | <td align=center></td>
| + | |
| - | <td align=center>1</td>
| + | |
| - | <td align=center>2</td>
| + | |
| - | </tr>
| + | |
| - | <tr>
| + | |
| - | <td align=center>3</td>
| + | |
| - | <td align=center>4</td>
| + | |
| - | <td align=center>5</td>
| + | |
| - | <td align=center>6</td>
| + | |
| - | <td align=center>7</td>
| + | |
| - | <td align=center>8</td>
| + | |
| - | <td align=center>9</td>
| + | |
| - | </tr>
| + | |
| - | <tr>
| + | |
| - | <td align=center>10</td>
| + | |
| - | <td align=center>11</td>
| + | |
| - | <td align=center>12</td>
| + | |
| - | <td align=center>13</td>
| + | |
| - | <td align=center>14</td>
| + | |
| - | <td align=center>15</td>
| + | |
| - | <td align=center>16</td>
| + | |
| - | </tr>
| + | |
| - | <tr>
| + | |
| - | <td align=center>17</td>
| + | |
| - | <td align=center>18</td>
| + | |
| - | <td align=center>19</td>
| + | |
| - | <td align=center>20</td>
| + | |
| - | <td align=center>21</td>
| + | |
| - | <td align=center>22</td>
| + | |
| - | <td align=center>23</td>
| + | |
| - | </tr>
| + | |
| - | <tr>
| + | |
| - | <td align=center>24</td>
| + | |
| - | <td align=center>25</td>
| + | |
| - | <td align=center>26</td>
| + | |
| - | <td align=center>27</td>
| + | |
| - | <td align=center>28</td>
| + | |
| - | <td align=center>29</td>
| + | |
| - | <td align=center>30</td>
| + | |
| - | </tr>
| + | |
| - | <tr>
| + | |
| - | <td align=center>31</td>
| + | |
| - | <td align=center></td>
| + | |
| - | <td align=center></td>
| + | |
| - | <td align=center></td>
| + | |
| - | <td align=center></td>
| + | |
| - | <td align=center></td>
| + | |
| - | <td align=center></td>
| + | |
| - | </tr>
| + | |
| - | </table>
| + | |
| - | | + | |
| - | <table>
| + | |
| - | <tr>
| + | |
| - | <td colspan="7"> September 2014 </td>
| + | |
| - | </tr>
| + | |
| - | <tr>
| + | |
| - | <td colspan="7" align=center><i> Month 6 of Project </i></td>
| + | |
| - | </tr>
| + | |
| - | <tr>
| + | |
| - | <td align=center>sun</td>
| + | |
| - | <td align=center>mon</td>
| + | |
| - | <td align=center>tue</td>
| + | |
| - | <td align=center>wed</td>
| + | |
| - | <td align=center>thu</td>
| + | |
| - | <td align=center>fri</td>
| + | |
| - | <td align=center>sat</td>
| + | |
| - | </tr>
| + | |
| - | <tr>
| + | |
| - | <td align=center></td>
| + | |
| - | <td align=center>1</td>
| + | |
| - | <td align=center>2</td>
| + | |
| - | <td align=center>3</td>
| + | |
| - | <td align=center>4</td>
| + | |
| - | <td align=center>5</td>
| + | |
| - | <td align=center>6</td>
| + | |
| - | </tr>
| + | |
| - | <tr>
| + | |
| - | <td align=center>7</td>
| + | |
| - | <td align=center>8</td>
| + | |
| - | <td align=center>9</td>
| + | |
| - | <td align=center>10</td>
| + | |
| - | <td align=center>11</td>
| + | |
| - | <td align=center>12</td>
| + | |
| - | <td align=center>13</td>
| + | |
| - | </tr>
| + | |
| - | <tr>
| + | |
| - | <td align=center>14</td>
| + | |
| - | <td align=center>15</td>
| + | |
| - | <td align=center>16</td>
| + | |
| - | <td align=center>17</td>
| + | |
| - | <td align=center>18</td>
| + | |
| - | <td align=center>19</td>
| + | |
| - | <td align=center>20</td>
| + | |
| - | </tr>
| + | |
| - | <tr>
| + | |
| - | <td align=center>21</td>
| + | |
| - | <td align=center>22</td>
| + | |
| - | <td align=center>23</td>
| + | |
| - | <td align=center>24</td>
| + | |
| - | <td align=center>25</td>
| + | |
| - | <td align=center>26</td>
| + | |
| - | <td align=center>27</td>
| + | |
| - | </tr>
| + | |
| - | <tr>
| + | |
| - | <td align=center>28</td>
| + | |
| - | <td align=center>29</td>
| + | |
| - | <td align=center>30</td>
| + | |
| - | <td align=center></td>
| + | |
| - | <td align=center></td>
| + | |
| - | <td align=center></td>
| + | |
| - | <td align=center></td>
| + | |
| - | </tr>
| + | |
| - | <tr>
| + | |
| - | <td align=center></td>
| + | |
| - | <td align=center></td>
| + | |
| - | <td align=center></td>
| + | |
| - | <td align=center></td>
| + | |
| - | <td align=center></td>
| + | |
| - | <td align=center></td>
| + | |
| - | <td align=center></td>
| + | |
| - | </tr>
| + | |
| - | </table>
| + | |
| - | | + | |
| - | <table>
| + | |
| - | <tr>
| + | |
| - | <td colspan="7"> October 2014 </td>
| + | |
| - | </tr>
| + | |
| - | <tr>
| + | |
| - | <td colspan="7" align=center><i> Month 7 of Project </i></td>
| + | |
| - | </tr>
| + | |
| - | <tr>
| + | |
| - | <td align=center>sun</td>
| + | |
| - | <td align=center>mon</td>
| + | |
| - | <td align=center>tue</td>
| + | |
| - | <td align=center>wed</td>
| + | |
| - | <td align=center>thu</td>
| + | |
| - | <td align=center>fri</td>
| + | |
| - | <td align=center>sat</td>
| + | |
| - | </tr>
| + | |
| - | <tr>
| + | |
| - | <td align=center></td>
| + | |
| - | <td align=center></td>
| + | |
| - | <td align=center></td>
| + | |
| - | <td align=center>1</td>
| + | |
| - | <td align=center>2</td>
| + | |
| - | <td align=center>3</td>
| + | |
| - | <td align=center>4</td>
| + | |
| - | </tr>
| + | |
| - | <tr>
| + | |
| - | <td align=center>5</td>
| + | |
| - | <td align=center>6</td>
| + | |
| - | <td align=center>7</td>
| + | |
| - | <td align=center>8</td>
| + | |
| - | <td align=center>9</td>
| + | |
| - | <td align=center>10</td>
| + | |
| - | <td align=center>11</td>
| + | |
| - | </tr>
| + | |
| - | <tr>
| + | |
| - | <td align=center>12</td>
| + | |
| - | <td align=center>13</td>
| + | |
| - | <td align=center>14</td>
| + | |
| - | <td align=center>15</td>
| + | |
| - | <td align=center>16</td>
| + | |
| - | <td align=center>17</td>
| + | |
| - | <td align=center>18</td>
| + | |
| - | </tr>
| + | |
| - | <tr>
| + | |
| - | <td align=center>19</td>
| + | |
| - | <td align=center>20</td>
| + | |
| - | <td align=center>21</td>
| + | |
| - | <td align=center>22</td>
| + | |
| - | <td align=center>23</td>
| + | |
| - | <td align=center>24</td>
| + | |
| - | <td align=center>25</td>
| + | |
| - | </tr>
| + | |
| - | <tr>
| + | |
| - | <td align=center>26</td>
| + | |
| - | <td align=center>27</td>
| + | |
| - | <td align=center>28</td>
| + | |
| - | <td align=center>29</td>
| + | |
| - | <td align=center>30</td>
| + | |
| - | <td align=center>31</td>
| + | |
| - | <td align=center></td>
| + | |
| - | </tr>
| + | |
| - | <tr>
| + | |
| - | <td align=center></td>
| + | |
| - | <td align=center></td>
| + | |
| - | <td align=center></td>
| + | |
| - | <td align=center></td>
| + | |
| - | <td align=center></td>
| + | |
| - | <td align=center></td>
| + | |
| - | <td align=center></td>
| + | |
| - | </tr>
| + | |
| - | </table>
| + | |
| - | </div>
| + | |
| - | </div>
| + | |
| - | | + | |
| - | <div id="rightnb">
| + | |
| - | | + | |
| - | | + | |
| - | <div class="day"> | + | |
| - | <a id="apr"><h1>April Overview</h1></a>
| + | |
| - | <div class="lab">
| + | |
| - | | + | |
| - | <a href="https://2014.igem.org/Team:Duke/Notebook/April"> Click here for the April Lab Notebook</a> | + | |
| - | | + | |
| - | <p>
| + | |
| - | The objective in April, while classes were still in session, was to make something called the Z4EV-dCas9-MXI1 construct. The model organism used was yeast, and this was a continuation of last year’s project.
| + | |
| - | </p>
| + | |
| - | <p>
| + | |
| - | The general idea was to fuse a repressor domain onto the dCas9 gene, to make an even stronger repressor. MXI1 is the repressor domain, and Z4EV is the promoter that was used. It’s originally a zinc-finger induced promoter for yeast.
| + | |
| - | </p>
| + | |
| - | <p>
| + | |
| - | This approach was abandoned because the general consensus was that repression is harder to achieve in yeast. Instead of trying to continue last year’s project, we decided to start afresh, with a new model organism, E. coli.
| + | |
| - | </p>
| + | |
| - | </div>
| + | |
| - | </div>
| + | |
| - | | + | |
| - | | + | |
| - | | + | |
| - | <div class="day">
| + | |
| - | <a id="mayjun"><h1>May, June Overview</h1></a>
| + | |
| - | <div class="obj">Objectives</div>
| + | |
| - | <div class="lab">
| + | |
| - | <p>This is what we were trying to accomplish in May and June. <a href="https://2014.igem.org/Team:Duke/Notebook/MayJun"> May and June Lab Notebook </a></p>
| + | |
| - | </div>
| + | |
| - | </div>
| + | |
| - | | + | |
| - | <div class="day">
| + | |
| - | <a id="jul"><h1> July Overview</h1></a>
| + | |
| - | <div class="obj">Objectives</div>
| + | |
| - | <div class="lab"> | + | |
| - | <p>This is what we were trying to accomplish in July. <a href="https://2014.igem.org/Team:Duke/Notebook/ July"> July Notebook </a> </p> | + | |
| - | </div> | + | |
| - | </div>
| + | |
| - | | + | |
| - | <div class="day"> | + | |
| - | <a id="aug"><h1>August Overview</h1></a>
| + | |
| - | <div class="obj">Objectives</div>
| + | |
| - | <div class="lab">
| + | |
| - | <p>This is what we were trying to accomplish in August. <a href="https://2014.igem.org/Team:Duke/Notebook/ August"> August Lab Notebook </a></p> | + | |
| - | </div>
| + | |
| - | </div>
| + | |
| - | | + | |
| - | | + | |
| - | <div class="day">
| + | |
| - | <a id="sept"><h1>September Overview</h1></a>
| + | |
| - | <div class="obj">Objectives</div>
| + | |
| - | <div class="lab">
| + | |
| - | <p>This is what we were trying to accomplish in September. <a href="https://2014.igem.org/Team:Duke/Notebook/September"> September Lab Notebook </a></p>
| + | |
| - | </div>
| + | |
| - | </div>
| + | |
| - | | + | |
| - | <div class="day">
| + | |
| - | <a id="oct"><h1>October Overview</h1></a>
| + | |
| - | <div class="obj">Objectives</div>
| + | |
| - | <div class="lab">
| + | |
| - | <p>This is what we were trying to accomplish in October. <a href="https://2014.igem.org/Team:Duke/Notebook/October"> October Lab Notebook </a></p>
| + | |
| - | </div>
| + | |
| - | </div>
| + | |
| - | | + | |
| - | <div class="day">
| + | |
| - | <a id="oct"><h1>Protocols</h1></a>
| + | |
| - | <div class="lab">
| + | |
| - | <p>Protocols can be found <a href="https://2014.igem.org/Team:Duke/Notebook/Protocols"> here </a></p>
| + | |
| - | </div>
| + | |
| - | </div>
| + | |
| - | | + | |
| - | | + | |
| - | </div> | + | |
| | </body> | | </body> |
| | </html> | | </html> |
"
