DNA Purification

• EZNA Miniprep
• Qiagen Gel Extraction
• Qiagen Miniprep
• Column Purification

PCR Methods

• Q5 PCR
• Phusion PCR
• Taq PCR
• Vent PCR

Metal and Growth Quantification

• Growth Experiments
• Batch Metal Sequestration Experiments
• Kinetic Metal Sequestration Experiments

Plasmid Insertion

• E.coli Electroporation
• Preparing Electrocompetent E.coli
• E.coli Heat Shock
• Preparing Chemically Compotent E.coli

DNA Assessment

• DNA Gel Electrophoresis
• Preparation of DNA for Sequencing

Plasmid Editing

• Double Digest
• Single Digest
• Site Directed Mutagenesis
• Vector Dephosphorylation
• T4 Ligase Ligation