Team:Brasil-SP/Notebook/Protocols

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<b>The content of this page is merely provisional!</b>
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<p>Well... the page title describe the page by itself!</p>
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<h1 align=center>Experimental Methodology</h1>
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<p>For the assemblies constructuion we addopted the following method:</p>
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<div align=center><img src="https://static.igem.org/mediawiki/2014/f/fc/Metodologia_Brasil-SP.png" width="900" height="400"/></div>
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<p>We used the pSB1C3 vector from the Biobrick <a href="http://parts.igem.org/Part:BBa_J04450">BBa_J04450</a>. Using this biobrick we added a red/white selection, where the red colonies are those which do not have the assembly, and the white ones are the correct ones.</p>
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<div align=center><img src="https://static.igem.org/mediawiki/2014/2/25/Red_and_WHite_plate_.jpg" width="700" height="265"/></div>
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<a href="https://2014.igem.org/Team:Brasil-SP"style="color:#000000">Home </a> </td>
 
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<a href="https://igem.org/Team.cgi?year=2014&team_name=Brasil-SP"style="color:#000000"> Official Team Profile </a></td>
 
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     <li><a href="https://static.igem.org/mediawiki/2014/7/74/PCR.pdf">PCR</a></li>
     <li><a href="https://static.igem.org/mediawiki/2014/7/74/PCR.pdf">PCR</a></li>
     <li><a href="https://static.igem.org/mediawiki/2014/a/af/Agarose_Gel_Electrophoresis_and_DNA_Gel_Purification.pdf">Agarose Gel Electrophoresis and DNA Gel Purification</a></li>
     <li><a href="https://static.igem.org/mediawiki/2014/a/af/Agarose_Gel_Electrophoresis_and_DNA_Gel_Purification.pdf">Agarose Gel Electrophoresis and DNA Gel Purification</a></li>
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     <li><a href="https://static.igem.org/mediawiki/2014/0/03/PCR_Product_Cloning.pdf">PCR Product cloning</a></li>
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     <li><a href="https://static.igem.org/mediawiki/2014/2/23/PCR_Product_Clonig_2.pdf">PCR Product cloning</a></li>
     <li><a href="https://static.igem.org/mediawiki/2014/9/96/Competent_Cells_using_Calcium_Chloride.pdf">Competent <i>E. coli</i> Cells using Calcium Chloride</a></li>
     <li><a href="https://static.igem.org/mediawiki/2014/9/96/Competent_Cells_using_Calcium_Chloride.pdf">Competent <i>E. coli</i> Cells using Calcium Chloride</a></li>
     <li><a href="https://static.igem.org/mediawiki/2014/a/a5/Transformation_in_Escherichia_coli_DH5.pdf">Transformation in <i>E. coli</i> DH5-alpha</a></li>
     <li><a href="https://static.igem.org/mediawiki/2014/a/a5/Transformation_in_Escherichia_coli_DH5.pdf">Transformation in <i>E. coli</i> DH5-alpha</a></li>
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     <li><a href="https://static.igem.org/mediawiki/2014/4/42/Restriction_Anaysis_and_Digestion_in_large_scale.pdf">Restriction Analysis and Digestion in large scale</a></li>
     <li><a href="https://static.igem.org/mediawiki/2014/4/42/Restriction_Anaysis_and_Digestion_in_large_scale.pdf">Restriction Analysis and Digestion in large scale</a></li>
     <li><a href="https://static.igem.org/mediawiki/2014/6/66/Bacillus_Competent_cells.pdf">Competent <i>Bacillus subtilis</i> cells</a></li>
     <li><a href="https://static.igem.org/mediawiki/2014/6/66/Bacillus_Competent_cells.pdf">Competent <i>Bacillus subtilis</i> cells</a></li>
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     <li><a href="https://static.igem.org/mediawiki/2014/8/84/Transformation_Bacilus.pdf">Transformation of <i>Bacillus subtilis</i></a></li>
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     <li><a href="https://static.igem.org/mediawiki/2014/a/a6/Transformation.pdf">Transformation of <i>Bacillus subtilis</i></a></li>
     <li><a href="https://static.igem.org/mediawiki/2014/3/34/DSM_Medium_Sporulation.pdf">DSM medium for sporulation</a></li>
     <li><a href="https://static.igem.org/mediawiki/2014/3/34/DSM_Medium_Sporulation.pdf">DSM medium for sporulation</a></li>
     <li><a href="https://static.igem.org/mediawiki/2014/0/00/Medium_LB.pdf">Luria-Bertani (LB) medium</a></li>
     <li><a href="https://static.igem.org/mediawiki/2014/0/00/Medium_LB.pdf">Luria-Bertani (LB) medium</a></li>
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     <li><a href="https://static.igem.org/mediawiki/2014/0/07/Neubauer_chamber.pdf">Cell counting with Neubauer Chamber</a></li>
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     <li><a href="https://static.igem.org/mediawiki/2014/0/0e/Neubauer_chamber_2.pdf">Cell counting with Neubauer Chamber</a></li>
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    <li><a href="https://static.igem.org/mediawiki/2014/a/a2/Germination_Sporulation.pdf">Germination of <i>Bacillus subtilis</i> spores</a></li>
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    <li><a href="https://static.igem.org/mediawiki/2014/6/63/QuickChange_PCR.pdf">QuickChange PCR</a></li>
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    <li><a href="https://static.igem.org/mediawiki/2014/3/35/PCR_for_synthesis.pdf">PCR for Synthesis</a></li>
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<div id="characterizationprotocols" class="row">
<div id="characterizationprotocols" class="row">
<h2 align="left"><b>Characterization Protocols</b></h2>
<h2 align="left"><b>Characterization Protocols</b></h2>
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    <li><a href="https://static.igem.org/mediawiki/2014/0/00/Cytometry_protocol_characterization.pdf">Cytometry Protocol</a></li>
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    <li><a href="https://static.igem.org/mediawiki/2014/0/02/Fluorometry_Protocol_Characterization.pdf">Fluorometry Protocol</a></li>
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Latest revision as of 23:38, 17 October 2014

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