Team:Brasil-SP/Notebook/Interlab Study

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Protocols Results Measurement Interlab Study As part of the first international interlab measurement study in synthetic biology, all Measurement Track teams were required to obtain fluorescence data for three specific genetic devices. They are
Biobrick device 1: BBa_I20260, an existing device Assembly Protocol Biobrick Device 1
Biobrick device 2: BBa_J23101 + BBa_E0240, a new device built by each team Assembly Protocol Biobrick Device 2 and 3
Biobrick device 3: BBa_J23115 + BBa_E0240, a new device built by each team. Note: Compared to biobrick device 1 and 2, biobrick device 3 was constructed with a different promoter (BBa_J23115). Assembly Protocol Biobrick Device 2 and 3
Flow Cytometry Protocol-Samples Preparation
Results For the proposed specific genetic devices, we have measured fluorescence by Flow Cytometry and Fluorometry. Device 1 and 2 are equivalent assemblies, however, we did not expect equal fluorescence values since device 1 was an existing device and 2 was built. When it comes to device 3, only the promoter was changed. Flow cytometry - Mean values

Protocols

Results