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Team:BGU Israel/Project/Hormone Workshop - Revision history
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Revision history for this page on the wiki
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Priels at 00:00, 18 October 2014
2014-10-18T00:00:28Z
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <h3 align="left" style="border-bottom:dashed;border-color:#000000">Mechanism </h3></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <h3 align="left" style="border-bottom:dashed;border-color:#000000">Mechanism </h3></div></td></tr>
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Priels
http://2014.igem.org/wiki/index.php?title=Team:BGU_Israel/Project/Hormone_Workshop&diff=355826&oldid=prev
Stav shamir at 21:25, 17 October 2014
2014-10-17T21:25:21Z
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<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div> <img src="https://static.igem.org/mediawiki/2014/1/12/BGU14adipo_figure_1.png" width="<del class="diffchange diffchange-inline">300</del>"/> </p></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div> <img src="https://static.igem.org/mediawiki/2014/1/12/BGU14adipo_figure_1.png" width="<ins class="diffchange diffchange-inline">330</ins>"/> </p></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <p align="center" style="font-size:14px; font-weight:bold;">Adiponectin oligomeric forms </div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <p align="center" style="font-size:14px; font-weight:bold;">Adiponectin oligomeric forms </div></td></tr>
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Stav shamir
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Stav shamir at 21:23, 17 October 2014
2014-10-17T21:23:54Z
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <h3 style="border-bottom:dashed;border-color:#000000">Background </h3></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <h3 style="border-bottom:dashed;border-color:#000000">Background </h3></div></td></tr>
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<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div> <p>Adiponectin is an adipocyte derived protein hormone showing insulin - sensitizing effect and has an important role in lipid and glucose metabolism regulation. Hypoadiponectemia (low levels of serum Adiponectin) is highly related with occurrence of abdominal obesity, insulin resistance, type 2 diabetes, and the metabolic syndrome. Adiponectin forms multimeric complexes that combine via its collagen domain to create three major oligomeric forms: a low molecular weight (LMW) trimer, a middle - molecular weight (MMW) hexamer, and high molecular weight (HMW) 12-18- mer Adiponectin. The form showing the most relevant properties considering insulin sensitivity and protection from diabetes is the HMW form of the hormone (Kadowaki et al., 2006). </div></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div> <p>Adiponectin is an adipocyte derived protein hormone showing insulin - sensitizing effect and has an important role in lipid and glucose metabolism regulation. Hypoadiponectemia (low levels of serum Adiponectin) is highly related with occurrence of abdominal obesity, insulin resistance, type 2 diabetes, and the metabolic syndrome. Adiponectin forms multimeric complexes that combine via its collagen domain to create three major oligomeric forms: a low molecular weight (LMW) trimer, a middle - molecular weight (MMW) hexamer, and high molecular weight (HMW) 12-18- mer Adiponectin. The form showing the most relevant properties considering insulin sensitivity and protection from diabetes is the HMW form of the hormone (Kadowaki et al., 2006). </div></td></tr>
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<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div> <p align="center" style="font-size:14px; font-weight:bold;"><del class="diffchange diffchange-inline">Figure 1. </del>Adiponectin oligomeric forms </div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div> <p align="center" style="font-size:14px; font-weight:bold;">Adiponectin oligomeric forms </div></td></tr>
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Stav shamir
http://2014.igem.org/wiki/index.php?title=Team:BGU_Israel/Project/Hormone_Workshop&diff=334862&oldid=prev
Tomye at 17:14, 17 October 2014
2014-10-17T17:14:43Z
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <h3 style="border-bottom:dashed;border-color:#000000">Mechanism <a href="#" onclick="goToByScroll('test'); return false;" class="right"></a></h3></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <h3 style="border-bottom:dashed;border-color:#000000">Mechanism <a href="#" onclick="goToByScroll('test'); return false;" class="right"></a></h3></div></td></tr>
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<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;">The most active form of the Adiponectin is a high molecular weight complex. The synthesis of the protein subunit and an enzyme for disulfide bonds should improve the levels of the active form of the hormone.</ins></div></td></tr>
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Tomye
http://2014.igem.org/wiki/index.php?title=Team:BGU_Israel/Project/Hormone_Workshop&diff=321657&oldid=prev
Priels at 13:38, 17 October 2014
2014-10-17T13:38:24Z
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>{{Team:BGU_Israel/Menu}}</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>{{Team:BGU_Israel/Menu}}</div></td></tr>
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<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;"> <p><b>The Problem: </b> Abnormal aggregation of fatty acid in non-adipose tissues, particularly skeletal muscles and liver </p></ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;"> <p><b>The Goal: </b>Reduce insulin resistance and improve the metabolism of metabolic syndrome patients</p></ins></div></td></tr>
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<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div> <p>Adiponectin is an <del class="diffchange diffchange-inline"> </del>adipocyte derived protein hormone showing insulin- sensitizing effect and <del class="diffchange diffchange-inline"> </del>important role in lipid and glucose metabolism regulation. Hypoadiponectemia (low <del class="diffchange diffchange-inline"> </del>levels of serum Adiponectin) is highly related with occurrence of abdominal <del class="diffchange diffchange-inline"> </del>obesity, insulin resistance, type 2 diabetes, and the metabolic syndrome. Adiponectin <del class="diffchange diffchange-inline"> </del>forms multimeric complexes that combine via its collagen domain to create three <del class="diffchange diffchange-inline"> </del>major oligomeric forms: a low molecular weight (LMW) trimer, a middle - <del class="diffchange diffchange-inline"> </del>molecular weight (MMW) hexamer, and high molecular weight (HMW) 12-18- mer <del class="diffchange diffchange-inline"> </del>Adiponectin. The form showing the most <del class="diffchange diffchange-inline"><div id="test"></div> </del>relevant properties considering insulin <del class="diffchange diffchange-inline"> </del>sensitivity and protection from diabetes is the HMW form of the hormone (<del class="diffchange diffchange-inline">1) (figure 1</del>.).<del class="diffchange diffchange-inline"></p> </del></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div> <p>Adiponectin is an adipocyte derived protein <ins class="diffchange diffchange-inline"> </ins>hormone showing insulin - sensitizing effect and <ins class="diffchange diffchange-inline">has an </ins>important role in lipid <ins class="diffchange diffchange-inline"> </ins>and glucose metabolism regulation. Hypoadiponectemia (low levels of serum <ins class="diffchange diffchange-inline"> </ins>Adiponectin) is highly related with occurrence of abdominal obesity, insulin <ins class="diffchange diffchange-inline"> </ins>resistance, type 2 diabetes, and the metabolic syndrome. Adiponectin forms multimeric <ins class="diffchange diffchange-inline"> </ins>complexes that combine via its collagen domain to create three major oligomeric <ins class="diffchange diffchange-inline"> </ins>forms: a low molecular weight (LMW) trimer, a middle - molecular weight (MMW) <ins class="diffchange diffchange-inline"> </ins>hexamer, and high molecular weight (HMW) 12-18- mer Adiponectin. The form <ins class="diffchange diffchange-inline"> </ins>showing the most relevant properties considering insulin sensitivity and <ins class="diffchange diffchange-inline"> </ins>protection from diabetes is the HMW form of the hormone (<ins class="diffchange diffchange-inline">Kadowaki et al</ins>.<ins class="diffchange diffchange-inline">, 2006</ins>). <ins class="diffchange diffchange-inline"> </ins></div></div></td></tr>
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<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div> <p>When <del class="diffchange diffchange-inline"> </del>one is diagnosed with the metabolic syndrome's symptoms and shows low levels of <del class="diffchange diffchange-inline"> </del>serum Adiponectin, an efficient treatment could be a pill or a shot containing <del class="diffchange diffchange-inline"> </del>the active HMW form of Adiponectin hormone. However, there is no known <del class="diffchange diffchange-inline"> </del>efficient synthetic method to produce the desired multimer structure of the <del class="diffchange diffchange-inline"> </del>hormone. It has been shown that overexpression of the enzyme DsbA-L (disulfide-bond <del class="diffchange diffchange-inline"> </del>A oxidoreductase - like), promotes Adiponectin multimerization, while suppressing <del class="diffchange diffchange-inline"> </del>its expression by RNAi markedly and selectively reduced Adiponectin levels and <del class="diffchange diffchange-inline"> </del>secretion in adipocytes (<del class="diffchange diffchange-inline">2</del>).<br></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div> <p>When one is diagnosed with the metabolic <ins class="diffchange diffchange-inline"> </ins>syndrome's symptoms and shows low levels of serum Adiponectin, an efficient <ins class="diffchange diffchange-inline"> </ins>treatment could be a pill or a shot containing the active HMW form of <ins class="diffchange diffchange-inline"> </ins>Adiponectin hormone. However, there is no known efficient synthetic method to <ins class="diffchange diffchange-inline"> </ins>produce the desired multimer structure of the hormone. It has been shown that overexpression <ins class="diffchange diffchange-inline"> </ins>of the enzyme DsbA-L (disulfide-bond A oxidoreductase - like), promotes <ins class="diffchange diffchange-inline"> </ins>Adiponectin multimerization, while suppressing its expression by RNAi markedly <ins class="diffchange diffchange-inline"> </ins>and selectively reduced Adiponectin levels and secretion in adipocytes (<ins class="diffchange diffchange-inline">Liu et al., 2008</ins>).<br></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del class="diffchange diffchange-inline"> </del>In <del class="diffchange diffchange-inline"> </del>order to synthesize the HMW form of Adiponectin, we intend to introduce <del class="diffchange diffchange-inline"> </del>overexpression of both Adiponectin and DsbA-L in our model cell line HepG2 <del class="diffchange diffchange-inline"> </del>(human hepatocellular carcinoma). This is expected to produces high levels of <del class="diffchange diffchange-inline"> </del>HMW Adiponectin <del class="diffchange diffchange-inline">(figure 2.) </del>while relying on the natural hormone secreting ability of <del class="diffchange diffchange-inline"> </del>hepatocytes and excretion tags on the hormone itself. <<del class="diffchange diffchange-inline">/p</del>></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline"> </ins>In order to synthesize the HMW form of Adiponectin, <ins class="diffchange diffchange-inline"> </ins>we intend to introduce overexpression of both Adiponectin and DsbA-L in our <ins class="diffchange diffchange-inline"> </ins>model cell line HepG2 (human hepatocellular carcinoma). This is expected to produces <ins class="diffchange diffchange-inline"> </ins>high levels of HMW Adiponectin while relying on the natural hormone secreting <ins class="diffchange diffchange-inline"> </ins>ability of hepatocytes and excretion tags on the hormone itself.<<ins class="diffchange diffchange-inline">br</ins>></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del class="diffchange diffchange-inline"><p></del>In the future, we intend to attach the hormone to an inducible system to allow for more control on the proteins synthesis. </p></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline"> </ins>In the future, we intend to attach the <ins class="diffchange diffchange-inline"> </ins>hormone to an inducible system to allow for more control on the proteins <ins class="diffchange diffchange-inline"> </ins>synthesis. </p></div></td></tr>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <h3 style="border-bottom:dashed;border-color:#000000">References </h3></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <h3 style="border-bottom:dashed;border-color:#000000">References </h3></div></td></tr>
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<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div> <p>Kadowaki, T. <del class="diffchange diffchange-inline">et al</del>. 2006 Adiponectin and adiponectin receptors in insulin resistance, diabetes, and the metabolic syndrome. J Clin Invest <del class="diffchange diffchange-inline">116:1784–1792 </del></<del class="diffchange diffchange-inline">p</del>></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div> <p>Kadowaki, T.<ins class="diffchange diffchange-inline">, Yamauchi, T</ins>.<ins class="diffchange diffchange-inline">, Kubota, N., Hara, K., Ueki, K., &amp; Tobe, K. (</ins>2006<ins class="diffchange diffchange-inline">). Review series </ins>Adiponectin and adiponectin receptors in <ins class="diffchange diffchange-inline"> </ins>insulin resistance , diabetes , and the metabolic syndrome. <ins class="diffchange diffchange-inline"><em></ins>J Clin Invest<ins class="diffchange diffchange-inline">.</ins></<ins class="diffchange diffchange-inline">em</ins>>, <em><ins class="diffchange diffchange-inline">116</ins></em><ins class="diffchange diffchange-inline">(7), 1784–1792</ins>. <ins class="diffchange diffchange-inline">doi:10.1172/JCI29126.1784<br><br></ins></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del class="diffchange diffchange-inline"> <p dir="LTR">Liu</del>, <del class="diffchange diffchange-inline">M.&nbsp;</del><em><del class="diffchange diffchange-inline">et al</del></em>.<del class="diffchange diffchange-inline">&nbsp;</del>2008.<del class="diffchange diffchange-inline">&nbsp;</del>A disulfide-bond A oxidoreductase-like protein <del class="diffchange diffchange-inline"> </del>(DsbA-L) regulates adiponectin multimerization.<del class="diffchange diffchange-inline">&nbsp;</del><em><del class="diffchange diffchange-inline">Proc. Natl. Acad. Sci. USA</del></em><del class="diffchange diffchange-inline">&nbsp;</del><<del class="diffchange diffchange-inline">strong</del>>105</<del class="diffchange diffchange-inline">strong</del>>:<del class="diffchange diffchange-inline">&nbsp;18302–18307</del>.</p></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline"> Liu, M., Zhou, L., Xu, A., Lam, K. S. L., Wetzel, M. D., Xiang, R., Liu, F. (</ins>2008<ins class="diffchange diffchange-inline">)</ins>. <ins class="diffchange diffchange-inline"> </ins>A disulfide-bond A oxidoreductase-like protein (DsbA-L) regulates adiponectin <ins class="diffchange diffchange-inline"> </ins>multimerization. <em><ins class="diffchange diffchange-inline">Proceedings of the National Academy of Sciences of the United States of America</ins></em><ins class="diffchange diffchange-inline">, </ins><<ins class="diffchange diffchange-inline">em</ins>>105</<ins class="diffchange diffchange-inline">em</ins>><ins class="diffchange diffchange-inline">(47), 18302–7. doi</ins>:<ins class="diffchange diffchange-inline">10.1073/pnas</ins>.<ins class="diffchange diffchange-inline">0806341105</ins></p></div></td></tr>
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Priels
http://2014.igem.org/wiki/index.php?title=Team:BGU_Israel/Project/Hormone_Workshop&diff=320010&oldid=prev
Priels at 12:59, 17 October 2014
2014-10-17T12:59:20Z
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <p align="center" style="border: thin solid; border-radius: 8px; background: #5FA6B8; font-style: normal; font-weight: 700; font-size: 24px; font-family: Allan, 'Hoefler Text', 'Liberation Serif', Times, 'Times New Roman', serif;"> Click on the picture to check out the machanism <img src="https://static.igem.org/mediawiki/2014/f/f5/BGU14finger-point2.png" width="26px"/></p></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <p align="center" style="border: thin solid; border-radius: 8px; background: #5FA6B8; font-style: normal; font-weight: 700; font-size: 24px; font-family: Allan, 'Hoefler Text', 'Liberation Serif', Times, 'Times New Roman', serif;"> Click on the picture to check out the machanism <img src="https://static.igem.org/mediawiki/2014/f/f5/BGU14finger-point2.png" width="26px"/></p></div></td></tr>
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<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;"> <div id="figure1" style="display:block"></ins></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <p align="center"></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <p align="center"></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del class="diffchange diffchange-inline"> </del><img <del class="diffchange diffchange-inline">id="figure1" </del>src="https://static.igem.org/mediawiki/2014/7/75/BGU14adipo_figure_2_a.png" onclick="change_pic_hormone()" width="400" style="border: thin solid; cursor: pointer;<del class="diffchange diffchange-inline">display</del>:<del class="diffchange diffchange-inline">block</del>" /></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline"> </ins><img src="https://static.igem.org/mediawiki/2014/7/75/BGU14adipo_figure_2_a.png" onclick="change_pic_hormone()" width="400" style="border: thin solid; cursor: pointer;<ins class="diffchange diffchange-inline">" /></ins></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div> <<del class="diffchange diffchange-inline">img </del>id="figure2" src="https://static.igem.org/mediawiki/2014/c/cb/BGU14adipo_figure_2_b.png" onclick="change_pic_hormone()" width="400" style="border: thin solid; cursor: pointer;<del class="diffchange diffchange-inline">display</del>:<del class="diffchange diffchange-inline">none</del>" /></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline"> </p></ins></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div> <<del class="diffchange diffchange-inline">img </del>id="figure3" src="https://static.igem.org/mediawiki/2014/1/1b/BGU14adipo_figure_2_c.png" onclick="change_pic_hormone()" width="400" style="border: thin solid; cursor: pointer;<del class="diffchange diffchange-inline">display:none</del>" /></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline"> <p align="center" style="font-size</ins>:<ins class="diffchange diffchange-inline">14px; font-weight:bold; line-height:normal</ins>"<ins class="diffchange diffchange-inline">>Adiponectin is overexpressed<</ins>/<ins class="diffchange diffchange-inline">p</ins>></div></td></tr>
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<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline"> <img </ins>src="https://static.igem.org/mediawiki/2014/c/cb/BGU14adipo_figure_2_b.png" onclick="change_pic_hormone()" width="400" style="border: thin solid; cursor: pointer;<ins class="diffchange diffchange-inline">" /></ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline"> </p></ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline"> <p align="center" style="font-size</ins>:<ins class="diffchange diffchange-inline">14px; font-weight:bold; line-height:normal</ins>"<ins class="diffchange diffchange-inline">>DsbA-L is overexpressed<</ins>/<ins class="diffchange diffchange-inline">p</ins>></div></td></tr>
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<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline"> <img </ins>src="https://static.igem.org/mediawiki/2014/1/1b/BGU14adipo_figure_2_c.png" onclick="change_pic_hormone()" width="400" style="border: thin solid; cursor: pointer;" /></div></td></tr>
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<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div> <p align="center" style="font-size:14px; font-weight:bold; line-height:normal"><del class="diffchange diffchange-inline">Figure 2 – Need </del>to <del class="diffchange diffchange-inline">put content</del></p></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div> <p align="center" style="font-size:14px; font-weight:bold; line-height:normal"><ins class="diffchange diffchange-inline">DsbA-L promotes Adiponectin multimerization leading </ins>to <ins class="diffchange diffchange-inline">high levels of HMW Adiponectin</ins></p></div></td></tr>
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Priels
http://2014.igem.org/wiki/index.php?title=Team:BGU_Israel/Project/Hormone_Workshop&diff=319883&oldid=prev
Priels at 12:55, 17 October 2014
2014-10-17T12:55:52Z
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <p align="center" style="font-size:14px; font-weight:bold;">Figure 1. Adiponectin oligomeric forms </div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <p align="center" style="font-size:14px; font-weight:bold;">Figure 1. Adiponectin oligomeric forms </div></td></tr>
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Priels
http://2014.igem.org/wiki/index.php?title=Team:BGU_Israel/Project/Hormone_Workshop&diff=319477&oldid=prev
Priels at 12:46, 17 October 2014
2014-10-17T12:46:36Z
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<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div> <img src="https://static.igem.org/mediawiki/2014/1/12/BGU14adipo_figure_1.png" /> </p></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div> <img src="https://static.igem.org/mediawiki/2014/1/12/BGU14adipo_figure_1.png" <ins class="diffchange diffchange-inline">width="400"</ins>/> </p></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <p align="center" style="font-size:14px; font-weight:bold;">Figure 1. Adiponectin oligomeric forms </div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <p align="center" style="font-size:14px; font-weight:bold;">Figure 1. Adiponectin oligomeric forms </div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> </p></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> </p></div></td></tr>
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Priels
http://2014.igem.org/wiki/index.php?title=Team:BGU_Israel/Project/Hormone_Workshop&diff=319419&oldid=prev
Priels at 12:45, 17 October 2014
2014-10-17T12:45:35Z
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<td colspan='2' style="background-color: white; color:black;">Revision as of 12:45, 17 October 2014</td>
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<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div> <img src="https://static.igem.org/mediawiki/2014/<del class="diffchange diffchange-inline">8</del>/<del class="diffchange diffchange-inline">83</del>/<del class="diffchange diffchange-inline">BGU14figure1-adiponectin</del>.png" /> </p></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div> <img src="https://static.igem.org/mediawiki/2014/<ins class="diffchange diffchange-inline">1</ins>/<ins class="diffchange diffchange-inline">12</ins>/<ins class="diffchange diffchange-inline">BGU14adipo_figure_1</ins>.png" <ins class="diffchange diffchange-inline"> </ins>/> </p></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <p align="center" style="font-size:14px; font-weight:bold;">Figure 1. Adiponectin oligomeric forms </div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <p align="center" style="font-size:14px; font-weight:bold;">Figure 1. Adiponectin oligomeric forms </div></td></tr>
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<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del class="diffchange diffchange-inline"> </del><img id="figure1" src="https://static.igem.org/mediawiki/2014/1/<del class="diffchange diffchange-inline">12</del>/<del class="diffchange diffchange-inline">BGU14adipo_figure_1</del>.png" onclick="change_pic_hormone()" width="400" style="border: thin solid; cursor: pointer;"></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline"> </ins><img id="figure1<ins class="diffchange diffchange-inline">" src="https://static.igem.org/mediawiki/2014/7/75/BGU14adipo_figure_2_a.png" onclick="change_pic_hormone()" width="400" style="border: thin solid; cursor: pointer;display:block" /></ins></div></td></tr>
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<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline"> <img id="figure3</ins>" src="https://static.igem.org/mediawiki/2014/1/<ins class="diffchange diffchange-inline">1b</ins>/<ins class="diffchange diffchange-inline">BGU14adipo_figure_2_c</ins>.png" onclick="change_pic_hormone()" width="400" style="border: thin solid; cursor: pointer;<ins class="diffchange diffchange-inline">display:none</ins>" <ins class="diffchange diffchange-inline">/</ins>></div></td></tr>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <p align="center" style="font-size:14px; font-weight:bold; line-height:normal">Figure 2 – Need to put content</p></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <p align="center" style="font-size:14px; font-weight:bold; line-height:normal">Figure 2 – Need to put content</p></div></td></tr>
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Priels
http://2014.igem.org/wiki/index.php?title=Team:BGU_Israel/Project/Hormone_Workshop&diff=306261&oldid=prev
Priels at 06:37, 17 October 2014
2014-10-17T06:37:40Z
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Priels