Team:Austin Texas/kit
From 2014.igem.org
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*+IPTG, -ncAA | *+IPTG, -ncAA | ||
*+IPTG, +ncAA | *+IPTG, +ncAA | ||
- | If the cultures did not have IPTG or an nCAA, an equal volume of sterile deionized water was added in order to keep the volumes between cultures constant. Once the water, the IPTG, and the ncAA were added appropriately, the cultures were allowed to grow to ~0.5 OD 600. 70 µL of each culture condition and control culture were added to a separate well in | + | If the cultures did not have IPTG or an nCAA, an equal volume of sterile deionized water was added in order to keep the volumes between cultures constant. Once the water, the IPTG, and the ncAA were added appropriately, the cultures were allowed to grow to ~0.5 OD 600. 70 µL of each culture condition and control culture were added to a separate well in clear-bottom black 96-well plate for fluorescence and OD readings using a fluorometer. |
Revision as of 16:57, 17 October 2014
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