Team:Austin Texas/kit
From 2014.igem.org
(→Using The Kit) |
(→Using The Kit) |
||
Line 154: | Line 154: | ||
IPTG stock solution was made at 1000X concentration ('''why is this here?''') and the ncAA was added to yield a concentration of 1 mM. Sterile deionized water was added in the place of ncAA and IPTG as a control ('''?'''). Once the controls, IPTG, and the ncAA were added appropriately, the cultures were allowed to grow to ~0.5 OD. 70 µL of each culture condition and control culture was added to a separate wells in a transparent 96-well plate for fluorescence and OD readings in a microplate reader. | IPTG stock solution was made at 1000X concentration ('''why is this here?''') and the ncAA was added to yield a concentration of 1 mM. Sterile deionized water was added in the place of ncAA and IPTG as a control ('''?'''). Once the controls, IPTG, and the ncAA were added appropriately, the cultures were allowed to grow to ~0.5 OD. 70 µL of each culture condition and control culture was added to a separate wells in a transparent 96-well plate for fluorescence and OD readings in a microplate reader. | ||
- | |||
[[File:10-14-14 big experiment test tubes.png|450px|thumb|left| Preparation for the ncAA Kit Test]] | [[File:10-14-14 big experiment test tubes.png|450px|thumb|left| Preparation for the ncAA Kit Test]] | ||
Revision as of 01:00, 15 October 2014
|