Team:Austin Texas/kit
From 2014.igem.org
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- | However, the above scenarios | + | However, the above scenarios assume that the synthetase/tRNA pair function efficiently and with high fidelity. |
- | + | *Actual synthetase/tRNA pairs sometimes have low fidelity, leading to the incorporation of an incorrect amino acid. Such a pair would not give results that mimic Figure 2C, expression of only RFP. Rather, in the absence of ncAA, such a pair would give results similar to Figure 2D, full expression of RFP and sfGFP. | |
+ | *Actual synthetase/tRNA pairs can also sometimes have low efficiency. As these are artificially selected pairs, sometimes they do not function as well as natural synthetase/tRNA pairs. In such a case, the amount of sfGFP translated in Figure 2D might be significantly lower than expected. | ||
Revision as of 19:41, 14 October 2014
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