Team:Aberdeen Scotland/Project

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<h1><i>E. coli</i>-based Trypanosomiasis Diagnostic System</h1>
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<h3>Our part in helping to develop Scottish national science policy in the area of synthetic biology</h3>
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<h3>Getting back at the Sleeping Sickness by detecting it early</h3>
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<p><i>E.coli </i> display of peptide mimotopes, integrated with quorum-sensing AND logic, for diagnosis of tropical diseases
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<p>The Aberdeen team was fortunate to be able to accept an invitation to an event on 16th October organised by the Chief Scientific Advisor of Scotland, Prof Muffy Calder. Prof. Calder is a Scottish government-appointed scientist whose role is to advise the Scottish government on science issues as they impact on government policy.</p>
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<i>Trypanosoma.b.gambiense</i>, the causative agent of Trypanosomiasis (African Sleeping Sickness) infects 30,000 people worldwide, with 60 million at disease risk.
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Unrecognised, this disease is fatal, but accurate diagnosis, relying on detection of patient serum antibodies against two different trypanosomal antigens, allows
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<p>Prof. Calder co-chairs the <a href="http://www.scottishscience.org.uk/">Scottish Scientific Advisory Council (SSAC)</a>, an government body providing scientific advice to government, and currently engaged with producing a report on the future development of synthetic biology as a tool for biotechnology in Scotland. We, the Aberdeen iGEM team were invited to the launch event for the SSAC Report on Synthetic biology: opportunities for Scotland, where we were asked to present a poster on our iGEM project to evidence the development of synthetic biology in Scotland. We had the opportunity to speak to a number of key figures in this area, including Prof Calder, and Prof. Nigel Brown, President of the Society for General Microbiology and co-author of the report. We considered that we had contributed in some small way to the development of synthetic biology policy, and were participants in the process of development of national science policy in this area. We regarded ourselves as privileged to see how national science policies, including those on synthetic biology are developed in our country, as well as to be able to speak to Scottish policy makers at the event.</p>
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successful treatment. We engineered autotransporters Antigen 43 and ice-nucleation protein to successfully express surface epitopes in <i>E.coli</i> that mimic two different
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trypanosomal antigens. Two <i>E.coli</i> strains were generated, each expressing a distinct surface epitope and either a quorum sensing (QS) sender or receiver module
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<p>The Scottish SSAC report on Synthetic biology: opportunities for Scotland: A report by the Scottish Science Advisory Council will be published on 17th September 2014.</p>
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respectively. Immune pull-down of each strain, and their subsequent co-culture, produced a quorum signal, indicating successful detection of two distinct antibody
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populations in a serum sample. We constructed a cheap, Raspberry Pi computer-controlled fluorimeter suitable for developing countries, showing it could detect QS
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<div class="float"><a href="http://www.scottishscience.org.uk/"><img src="https://static.igem.org/mediawiki/2014/9/90/SSAClogo.png"></a></div>
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fluorescence output. This novel application of antigen display, and quorum sensing to implement AND logic sensing, facilitates cheap, simple diagnosis of tropical
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diseases.</p>
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<h4>iGEM Aberdeen: public outreach and science communication</h4>
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<p>The Aberdeen team used a number of outreach opportunities to engage with non-scientists and the general public. Each provided the opportunity to engage with a lay audience, and find out what the public thinks of the science of synthetic biology. We discussed our science and presented at the following events.</p>
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<p><b>SCHMU Radio, Aberdeen:</b> our team was interviewed on the SHMU local radio station for 20 minutes on the Talking Science show, giving us the chance to communicate our project, and the concept of synthetic biology, to an audience in the Aberdeen city area.</p>
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<p><b>Explorathon 2014:</b> this annual European event is a vast science public outreach event giving researchers from across Europe the chance to showcase their science to a general public audience. On <a href="http://www.explorathon.co.uk/aberdeen">Explorathon day</a> (26th September 2014), our contribution was present to an open audience in a quick-fire Pecha Kucha event (20 slides, 20 seconds each) held in the Belmont cinema in Aberdeen city centre. This open event was attended by a large number of members of the public, followed by a Q&A session.</p>
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<p><b>iGEM project blog with <a href="http://www.aumag.co.uk/">AU Magazine</a>:</b> our team had a great opportunity to engage with non-scientists through a 3-part blog we produced in the Aberdeen University science magazine 'Au'. This magazine is written for a non-scientific audience, providing an important outreach opportunity.</p>
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<h5>Our goal for publishing a series of articles was to:</h5>
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<li>Address the general public's misconceptions surrounding synthetic biology</li>
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<li>Get people thinking about how synthetic biology can provide solutions to serious global issues we face nowadays</li>
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<li>To start a discussion about the ethical implication of our actions; Our moral responsibility to use knowledge to do good, as well as looking at the sustainability of synthetic biology in the future</li>
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<li>To inspire readers of the magazine (fellow students) and show that young people can make a positive contribution and tackle global problems</li>
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<h4>To date we have published 2 articles:</h4>
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<h5><b><a href="https://static.igem.org/mediawiki/2014/9/9e/A_Young_Physicist.pdf">A Young Physicist’s perspective on Synthetic Biology</a></b>, in which we addressed the following:</h5>
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<li>- What is iGEM</li>
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<li>- What is synthetic biology, emphasizing its interdisciplinary aspect and debunking myths surrounding 'the Frankenstein's biology'</li>
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<li>- Why are physicists involved in biology projects? How the modelling can guide informed design of biological research</li>
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<li>- The unlimited potential of synbio and how we implement it in everyday life</li>
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<li>- The risks and ethical implications connected with irresponsible applications of synbio, and how it is tackled by laws and regulations</li>
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<h5><b>Wake up to the sleeping sickness!</b>, our grand plan to engineer an E. coli System for the diagnosis of neglected tropical diseases:</h5>
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<li>- What is Human African Trypanosomiasis (HAT)</li>
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<li>- The burden of the disease</li>
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<li>- How to engineer a diagnostic system adapted to function effectively in remote parts of Africa and how synthetic biology enables us to do so</li>
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<li>- The solution - our E.coli operated diagnosis system and a HAT detector device</li>
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<p>Our third article about the Giant iGEM Jamboree and an exchange of ideas within an international scientific community will be published in mid-November. We will emphasize the international aspect of the competition and how all teams work together to perform high quality, safe science.</p>
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<h4>Interactions with the Scottish Health Service</h4>
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<p>In addition to the above outreach, as part of the project planning we also contacted a nearby National Health Service lab (Ms Leslie Bevridge, Grampian health authority Microbiology pathology lab) to identify possible disease targets for a new diagnosis tool. While we eventually settled on tropical disease diagnosis in remote areas we were still periodically in contact throughout the project, often regarding our weekly progress.</p>
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Latest revision as of 02:33, 18 October 2014

Team:Aberdeen Scotland/Project - 2014.ogem.org



E. coli-based Trypanosomiasis Diagnostic System

Getting back at the Sleeping Sickness by detecting it early


E.coli display of peptide mimotopes, integrated with quorum-sensing AND logic, for diagnosis of tropical diseases Trypanosoma.b.gambiense, the causative agent of Trypanosomiasis (African Sleeping Sickness) infects 30,000 people worldwide, with 60 million at disease risk. Unrecognised, this disease is fatal, but accurate diagnosis, relying on detection of patient serum antibodies against two different trypanosomal antigens, allows successful treatment. We engineered autotransporters Antigen 43 and ice-nucleation protein to successfully express surface epitopes in E.coli that mimic two different trypanosomal antigens. Two E.coli strains were generated, each expressing a distinct surface epitope and either a quorum sensing (QS) sender or receiver module respectively. Immune pull-down of each strain, and their subsequent co-culture, produced a quorum signal, indicating successful detection of two distinct antibody populations in a serum sample. We constructed a cheap, Raspberry Pi computer-controlled fluorimeter suitable for developing countries, showing it could detect QS fluorescence output. This novel application of antigen display, and quorum sensing to implement AND logic sensing, facilitates cheap, simple diagnosis of tropical diseases.