Team:Aberdeen Scotland/Parts
From 2014.igem.org
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<h1>Background to Parts Design</h1> | <h1>Background to Parts Design</h1> | ||
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- | <p>Antigen 43 (Ag43) | + | <p><b>Antigen 43</b> (sometimes called Ag43 or fluffing protein) is a phase-variable outer membrane protein encoded by flu gene. It is native to E.Coli K12 strain and is usually expressed at about 50, 000 copies/cell. Ag34 precursor is 1039 amino acids long and subsequently becomes cleaved into alpha and beta chains (499 and 488 amino acids long respectively). The beta subunit forms a β-barrel pore via which alpha-subunit translocates to the cell surface, and with which it remains non-covalently joined. The surface alpha chain can be released by a brief heat treatment at approx. 60<sup>o</sup>C.</p> |
<img src="https://static.igem.org/mediawiki/2014/2/2e/Ag43.jpg" alt="Ag43"> | <img src="https://static.igem.org/mediawiki/2014/2/2e/Ag43.jpg" alt="Ag43"> | ||
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Revision as of 02:26, 17 October 2014
Background to Parts Design
Antigen 43 (sometimes called Ag43 or fluffing protein) is a phase-variable outer membrane protein encoded by flu gene. It is native to E.Coli K12 strain and is usually expressed at about 50, 000 copies/cell. Ag34 precursor is 1039 amino acids long and subsequently becomes cleaved into alpha and beta chains (499 and 488 amino acids long respectively). The beta subunit forms a β-barrel pore via which alpha-subunit translocates to the cell surface, and with which it remains non-covalently joined. The surface alpha chain can be released by a brief heat treatment at approx. 60oC.