Team:Aachen/Project/FRET Reporter
From 2014.igem.org
(→Characterization of GFP-REACh1 and GFP-REACh2 fusion proteins in combination with an IPTG-inducible TEV Protease) |
m (→Comparing the kinetic of the double plasmid systems K1319013 + K1319008 and K1319014 + K1319008 with standard GFP expression) |
||
(4 intermediate revisions not shown) | |||
Line 15: | Line 15: | ||
<li style="width: 156px;margin-left: 8px;margin-right: 8px;"> | <li style="width: 156px;margin-left: 8px;margin-right: 8px;"> | ||
<a class="menulink" href="https://2014.igem.org/Team:Aachen/Project/FRET_Reporter#fluorescence" style="color:black"> | <a class="menulink" href="https://2014.igem.org/Team:Aachen/Project/FRET_Reporter#fluorescence" style="color:black"> | ||
- | <div class="menusmall-item menusmall-info" ><div class="menukachel" style="line-height: 1.5em | + | <div class="menusmall-item menusmall-info" ><div class="menukachel" style="top:32%; line-height:1.5em;">A Faster Answer</div></div> |
<div class="menusmall-item menusmall-img" style="background: url(https://static.igem.org/mediawiki/2014/0/0b/Aachen_14-10-13_GFP_iNB.png); norepeat scroll 0% 0% transparent; background-size:100%"> | <div class="menusmall-item menusmall-img" style="background: url(https://static.igem.org/mediawiki/2014/0/0b/Aachen_14-10-13_GFP_iNB.png); norepeat scroll 0% 0% transparent; background-size:100%"> | ||
</div> | </div> | ||
Line 23: | Line 23: | ||
<li style="width: 156px;margin-left: 8px;margin-right: 8px;"> | <li style="width: 156px;margin-left: 8px;margin-right: 8px;"> | ||
<a class="menulink" href="https://2014.igem.org/Team:Aachen/Project/FRET_Reporter#fret" style="color:black"> | <a class="menulink" href="https://2014.igem.org/Team:Aachen/Project/FRET_Reporter#fret" style="color:black"> | ||
- | <div class="menusmall-item menusmall-info" ><div class="menukachel" style="line-height: 1.5em | + | <div class="menusmall-item menusmall-info" ><div class="menukachel" style="top:32%; line-height:1.5em;">The FRET System</div></div> |
<div class="menusmall-item menusmall-img" style="background: url(https://static.igem.org/mediawiki/2014/5/54/Aachen_14-10-13_FRET_Arrows_iNB.png); norepeat scroll 0% 0% transparent; background-size:100%"> | <div class="menusmall-item menusmall-img" style="background: url(https://static.igem.org/mediawiki/2014/5/54/Aachen_14-10-13_FRET_Arrows_iNB.png); norepeat scroll 0% 0% transparent; background-size:100%"> | ||
</div> | </div> | ||
Line 31: | Line 31: | ||
<li style="width: 156px;margin-left: 8px;margin-right: 8px;"> | <li style="width: 156px;margin-left: 8px;margin-right: 8px;"> | ||
<a class="menulink" href="https://2014.igem.org/Team:Aachen/Project/FRET_Reporter#darkquencher" style="color:black"> | <a class="menulink" href="https://2014.igem.org/Team:Aachen/Project/FRET_Reporter#darkquencher" style="color:black"> | ||
- | <div class="menusmall-item menusmall-info" ><div class="menukachel" style="line-height: 1.5em | + | <div class="menusmall-item menusmall-info" ><div class="menukachel" style="top:32%; line-height:1.5em;">REACh Quenchers</div></div> |
<div class="menusmall-item menusmall-img" style="background: url(https://static.igem.org/mediawiki/2014/6/65/Aachen_14-10-13_REACh_iNB.png); norepeat scroll 0% 0% transparent; background-size:100%"> | <div class="menusmall-item menusmall-img" style="background: url(https://static.igem.org/mediawiki/2014/6/65/Aachen_14-10-13_REACh_iNB.png); norepeat scroll 0% 0% transparent; background-size:100%"> | ||
</div> | </div> | ||
Line 39: | Line 39: | ||
<li style="width: 156px;margin-left: 8px;margin-right: 8px;"> | <li style="width: 156px;margin-left: 8px;margin-right: 8px;"> | ||
<a class="menulink" href="https://2014.igem.org/Team:Aachen/Project/FRET_Reporter#gfp-reach" style="color:black"> | <a class="menulink" href="https://2014.igem.org/Team:Aachen/Project/FRET_Reporter#gfp-reach" style="color:black"> | ||
- | <div class="menusmall-item menusmall-info" ><div class="menukachel" style="line-height: 1.5em | + | <div class="menusmall-item menusmall-info" ><div class="menukachel" style="top:32%; line-height:1.5em;">The Fusion Protein</div></div> |
<div class="menusmall-item menusmall-img" style="background: url(https://static.igem.org/mediawiki/2014/0/02/Aachen_14-10-13_Fusion_Protein_iNB.png); norepeat scroll 0% 0% transparent; background-size:100%"> | <div class="menusmall-item menusmall-img" style="background: url(https://static.igem.org/mediawiki/2014/0/02/Aachen_14-10-13_Fusion_Protein_iNB.png); norepeat scroll 0% 0% transparent; background-size:100%"> | ||
</div> | </div> | ||
Line 54: | Line 54: | ||
<li style="width: 156px;margin-left: 8px;margin-right: 8px;"> | <li style="width: 156px;margin-left: 8px;margin-right: 8px;"> | ||
<a class="menulink" href="https://2014.igem.org/Team:Aachen/Project/FRET_Reporter#tevprotease" style="color:black"> | <a class="menulink" href="https://2014.igem.org/Team:Aachen/Project/FRET_Reporter#tevprotease" style="color:black"> | ||
- | <div class="menusmall-item menusmall-info" ><div class="menukachel" style="line-height: 1.5em | + | <div class="menusmall-item menusmall-info" ><div class="menukachel" style="top:32%; line-height:1.5em;">TEV Protease</div></div> |
<div class="menusmall-item menusmall-img" style="background: url(https://static.igem.org/mediawiki/2014/d/dc/Aachen_14-10-13_TEV_Protease_iNB.png); norepeat scroll 0% 0% transparent; background-size:100%"> | <div class="menusmall-item menusmall-img" style="background: url(https://static.igem.org/mediawiki/2014/d/dc/Aachen_14-10-13_TEV_Protease_iNB.png); norepeat scroll 0% 0% transparent; background-size:100%"> | ||
</div> | </div> | ||
Line 62: | Line 62: | ||
<li style="width: 156px;margin-left: 8px;margin-right: 8px;"> | <li style="width: 156px;margin-left: 8px;margin-right: 8px;"> | ||
<a class="menulink" href="https://2014.igem.org/Team:Aachen/Project/FRET_Reporter#reachachievements" style="color:black"> | <a class="menulink" href="https://2014.igem.org/Team:Aachen/Project/FRET_Reporter#reachachievements" style="color:black"> | ||
- | <div class="menusmall-item menusmall-info" ><div class="menukachel" style="line-height: 1.5em | + | <div class="menusmall-item menusmall-info" ><div class="menukachel" style="top:32%; line-height:1.5em;">Achieve-<br/>ments</div></div> |
<div class="menusmall-item menusmall-img" style="background: url(https://static.igem.org/mediawiki/2014/e/ef/Aachen_14-10-15_Medal_Cellocks_iNB.png); norepeat scroll 0% 0% transparent; background-size:100%"> | <div class="menusmall-item menusmall-img" style="background: url(https://static.igem.org/mediawiki/2014/e/ef/Aachen_14-10-15_Medal_Cellocks_iNB.png); norepeat scroll 0% 0% transparent; background-size:100%"> | ||
</div> | </div> | ||
Line 70: | Line 70: | ||
<li style="width: 156px;margin-left: 8px;margin-right: 8px;"> | <li style="width: 156px;margin-left: 8px;margin-right: 8px;"> | ||
<a class="menulink" href="https://2014.igem.org/Team:Aachen/Project/FRET_Reporter#reachoutlook" style="color:black"> | <a class="menulink" href="https://2014.igem.org/Team:Aachen/Project/FRET_Reporter#reachoutlook" style="color:black"> | ||
- | <div class="menusmall-item menusmall-info" ><div class="menukachel" style=" | + | <div class="menusmall-item menusmall-info" ><div class="menukachel" style="top:40%;">Outlook</div></div> |
<div class="menusmall-item menusmall-img" style="background: url(https://static.igem.org/mediawiki/2014/6/67/Aachen_14-10-16_Outlook_Cellocks_iNB.png); norepeat scroll 0% 0% transparent; background-size:100%"> | <div class="menusmall-item menusmall-img" style="background: url(https://static.igem.org/mediawiki/2014/6/67/Aachen_14-10-16_Outlook_Cellocks_iNB.png); norepeat scroll 0% 0% transparent; background-size:100%"> | ||
</div> | </div> | ||
Line 158: | Line 158: | ||
{{Team:Aachen/Figure|align=center|Aachen 14-10-17 IPTG REACh iFG.png|title= Composition of our biosensor for IPTG|subtitle=To test the funtionality of our sensor concept, we expressed the TEV protease with a T7 promoter.|width=900px}} | {{Team:Aachen/Figure|align=center|Aachen 14-10-17 IPTG REACh iFG.png|title= Composition of our biosensor for IPTG|subtitle=To test the funtionality of our sensor concept, we expressed the TEV protease with a T7 promoter.|width=900px}} | ||
+ | |||
{{Team:Aachen/BlockSeparator}} | {{Team:Aachen/BlockSeparator}} | ||
Line 254: | Line 255: | ||
|<html> <img src="https://static.igem.org/mediawiki/2014/1/1a/Aachen_K13%2B8%2CK14%2B8%2CK731_slower_reduced.gif" width="480px"></html> | |<html> <img src="https://static.igem.org/mediawiki/2014/1/1a/Aachen_K13%2B8%2CK14%2B8%2CK731_slower_reduced.gif" width="480px"></html> | ||
|- | |- | ||
- | |'''{{{title|K1319013 + K1319008, K1319014 + K1319008 and K731520 in an non-induced (top) and induced (bottom) chip}}}'''<br />{{{subtitle|Comparing the factor of fluorescence | + | |'''{{{title|K1319013 + K1319008, K1319014 + K1319008 and K731520 in an non-induced (top) and induced (bottom) chip}}}'''<br />{{{subtitle|Comparing the factor of fluorescence increase of induced (bottom) and not induced (top) sensor chips to the first frame of the measurement. The tested constructs are K1319013 + K1319008, K1319014 + K1319008 and K731520. After five hours, the fluorescence in our dual-plasmid REACh construct contaning K1319014 had increased by a factor of about 8, while K731520 fluorescence increased by only a factor of 2-3.}}} |
|} | |} | ||
</div> | </div> |
Latest revision as of 03:47, 18 October 2014
|
|
|
|
|
|
|
|
|