Team:Aachen/Notebook/Engineering/ODF
From 2014.igem.org
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It can be seen that the platereader show a much higher difference between the GFP and non-GFP cell culture at a higher standard deviation. | It can be seen that the platereader show a much higher difference between the GFP and non-GFP cell culture at a higher standard deviation. | ||
Another interesting metric is the difference between the GFP and non-GFP, which can be seen as the normalized fluorescence measure. | Another interesting metric is the difference between the GFP and non-GFP, which can be seen as the normalized fluorescence measure. | ||
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+ | <center> | ||
+ | {{Team:Aachen/Figure|align=center|Aachen_Fluoro_GFP_PODF_abs.png|title=Fluorescence Measurement comparison OD/F device and Platereader|subtitle=Comparison of a fluorescence measurement of our device and the platereader. Our OD/F device shows no significant.|width=900px}} | ||
+ | {{Team:Aachen/BlockSeparator}} | ||
+ | </center> | ||
If one compares the results there, as in Figure 2, interesting observations can be made. | If one compares the results there, as in Figure 2, interesting observations can be made. | ||
First, both platereader and OD/F device show very similar results. | First, both platereader and OD/F device show very similar results. | ||
The regression curves differ only in a linear factor. | The regression curves differ only in a linear factor. | ||
- | Most interestingly general fit of the OD/F device to a linear function seems to be better than | + | Most interestingly the general fit of the OD/F device to a linear function seems to be better than the platereader. |
Overall the linearity which has been observed earlier (in testing the general setup) could be verified. | Overall the linearity which has been observed earlier (in testing the general setup) could be verified. | ||
Therefore our do-it-yourself OD/F device can be used to determine fluorescence. | Therefore our do-it-yourself OD/F device can be used to determine fluorescence. | ||
+ | At higher concentrations our OD/F device struggles regarding accuracy. However, this is also true for the platereader, but at a lower rate. | ||
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+ | <center> | ||
+ | {{Team:Aachen/Figure|align=center|Aachen_Fluoro_GFP_PODF_rel.png|title=Fluorescence Measurement comparison OD/F device and Platereader|subtitle=Comparison of a fluorescence measurement of our device and the platereader. Our OD/F device shows no significant. A non-GFP expressing E. coli dilution has been used to normalize the GFP dilution series. A linear correlation can be seen.|width=900px}} | ||
+ | {{Team:Aachen/BlockSeparator}} | ||
+ | </center> | ||
Revision as of 22:42, 13 October 2014
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