Team:Aachen/Notebook/Engineering/ODF
From 2014.igem.org
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== Evaluation == | == Evaluation == | ||
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+ | We performed several experiments during the development of the OD/F device. | ||
+ | Finally we can relate the measured transmittance to the true Optical Density, and further, we can relate that true OD to the one of the photospectrometer in our lab. | ||
+ | By doing to we can calibrate our device to meaningful values. | ||
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+ | We have done this according to the previous section for Pseudomonas putida and Saccharomyces cerevisiae. | ||
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+ | The final function for calculating the OD from the transmission calculated by our device can be calculated as | ||
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+ | $$ OD = f(T) \circ g(device) $$ | ||
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+ | where $f$ transforms transforms transmittance to true optical density for our device, and $g$ transforms true optical density of our device into the true optical density of the photospectrometer. This way our device is calibrated according to the photospectrometer. | ||
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+ | === Pseudomonas putida === | ||
+ | <center> | ||
+ | {{Team:Aachen/Figure|align=center|Aachen_Fluoro_CharCurve_Linearity.png|title=Linearity of TSL235R-LF sensor|subtitle=Dilution series of GFP expressing E. coli showing linearity between fluorescence count and dilution.|width=900px}} | ||
+ | </center> | ||
+ | |||
+ | <center> | ||
+ | {{Team:Aachen/Figure|align=center|Aachen_Fluoro_CharCurve_Linearity.png|title=Linearity of TSL235R-LF sensor|subtitle=Dilution series of GFP expressing E. coli showing linearity between fluorescence count and dilution.|width=900px}} | ||
+ | </center> | ||
+ | |||
+ | === Saccharomyces cerevisiae === | ||
+ | |||
+ | <center> | ||
+ | {{Team:Aachen/Figure|align=center|Aachen_SCer_T_TOD.png|title=Linearity of TSL235R-LF sensor|subtitle=Dilution series of GFP expressing E. coli showing linearity between fluorescence count and dilution.|width=900px}} | ||
+ | </center> | ||
+ | |||
+ | <center> | ||
+ | {{Team:Aachen/Figure|align=center|Aachen_SCer_TOD_TOD.png|title=Linearity of TSL235R-LF sensor|subtitle=Dilution series of GFP expressing E. coli showing linearity between fluorescence count and dilution.|width=900px}} | ||
+ | </center> | ||
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+ | From these plots it can first be seen that our device delivers robust and reproducible results for both procaryotes and eucaryotes. | ||
+ | Also the function from transmittance to true od can be expressed as a lower polynomial function, making its calculation easily possible on a low-end device like a microcontroller. | ||
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+ | Most encouraging is that the function for relating the true OD of our device to the photospectrometer is, as seen by the regression coefficient, close together for both P. putida and S. cerivisae. In fact, 3.4199 and 3.4446 are such close together, that the minor deviation could be just measuring inaccuracy. | ||
+ | Therefore we fix the regression coefficient for converting true OD of our device to true OD of the photospectrometer to 3.432 . | ||
== DIY: How to Build Your Own OD Device== | == DIY: How to Build Your Own OD Device== |
Revision as of 23:25, 13 October 2014
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