Team:Aachen/Labbook

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In order to detect the pathogens fast, specifically and inexpensively we are building sensor cells to detect these pathogens. These sensor cells can identify pathogens in very low concentration by responsing to specific extracellular molecules either secreted by or displayed on the pathogens. These molecules trigger a fast fluorescence response by our immobilized sensor cells which will be measured by our device. Aachen Cellock standingup.png


More information will be available soon!

May

1st to 11th

Thu, 8th

  • SOE-PCR step 2

12th to 18th

  • ...

23rd

  • we transformed some BioBricks and did a colony PCR
colony PCR picture

26th to 31st

  • ...


June

25th

  • preculture for competent NEB10β cells
  • submit samples for sequencing
  • make masterplates of transformed BioBricks
  • centrifuge and freeze overnight expression culture of J23101.E0240 and K516132

26th

  • made competent NEB10β cells - several things went wrong.. we'll see (remember: pre-cool centrifuge, always check if it's indeed spinning, frequently check OD of the culture)
  • colony PCR on the transformed clones looked awful. There were too many cells in the 10 µl reaction volume. Some seals weren't fully closed. Basically only primers and smear except for the positive control which contained a plasmid template instead of cells
  • 2x 500 ml of LB and three sterile flasks have been made

July

16th

  • plasmid preps
  • transformation of different reporter strains
  • ...

sample table:

BioBrick Colonies after Transformation Colony PCR result
E0240 5 positive
E0040 6 negative

August

...

September

...

October

...



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