Team:Aachen/Labbook

From 2014.igem.org

(Difference between revisions)
m (July)
m (May)
Line 17: Line 17:
= May =
= May =
-
== 1st to 11th ==
+
=== 8th ===
-
=== Thu, 8th ===
+
* SOE-PCR step 2
* SOE-PCR step 2
-
== 12th to 18th ==
 
-
* ...
 
== 23rd ==
== 23rd ==
* we transformed some BioBricks and did a colony PCR
* we transformed some BioBricks and did a colony PCR
Line 30: Line 27:
</html>
</html>
-
== 26th to 31st ==
 
-
* ...
 
{{Team:Aachen/JumpUp}}
{{Team:Aachen/JumpUp}}
 +
= June =
= June =
== 25th ==
== 25th ==

Revision as of 12:26, 23 July 2014

Contents

In order to detect the pathogens fast, specifically and inexpensively we are building sensor cells to detect these pathogens. These sensor cells can identify pathogens in very low concentration by responsing to specific extracellular molecules either secreted by or displayed on the pathogens. These molecules trigger a fast fluorescence response by our immobilized sensor cells which will be measured by our device. Aachen Cellock standingup.png


More information will be available soon!

May

8th

  • SOE-PCR step 2

23rd

  • we transformed some BioBricks and did a colony PCR
colony PCR picture












June

25th

  • preculture for competent NEB10β cells
  • submit samples for sequencing
  • make masterplates of transformed BioBricks
  • centrifuge and freeze overnight expression culture of J23101.E0240 and K516132

26th

  • made competent NEB10β cells - several things went wrong.. we'll see (remember: pre-cool centrifuge, always check if it's indeed spinning, frequently check OD of the culture)
  • colony PCR on the transformed clones looked awful. There were too many cells in the 10 µl reaction volume. Some seals weren't fully closed. Basically only primers and smear except for the positive control which contained a plasmid template instead of cells
  • 2x 500 ml of LB and three sterile flasks have been made

July

16th

  • plasmid preps
  • transformation of different reporter strains
  • ...

sample table:

BioBrick Colonies after Transformation Colony PCR result
E0240 5 positive
E0040 6 negative


22nd

  • Philipp and Michael made 100x stocks for Hartmanns media
  • Michael and Vera made about 25 HM+C-plates without Glucose
  • K731520 iLOV was plated on a HM+C plate

23rd

  • Michael made 25 new HM+C plates with 1 % agar and 4 g/L glucose
  • He also added 170 µl of the Glucose stock (500 g/L) to the Glucose-free HM+C-plates

August

...

September

...

October

...