# Team:Aachen/Interlab Study/Hardware

(Difference between revisions)
 Revision as of 22:32, 22 August 2014 (view source)Mjoppich (Talk | contribs) (→Open Source DIY Hardware)← Older edit Revision as of 22:35, 22 August 2014 (view source)Mjoppich (Talk | contribs) (→Open Source DIY Hardware)Newer edit → Line 24: Line 24: This cuvette holder can be used for both devices: the whole in the bottom is used for fluorescence measurement, the two opposite wholes are used for the light sensor and the LED for optical density measurement respectively. This cuvette holder can be used for both devices: the whole in the bottom is used for fluorescence measurement, the two opposite wholes are used for the light sensor and the LED for optical density measurement respectively. + + === Hardware Requirements === The key ingredients for our combined optical density/fluorescence (OD/F) device then are: The key ingredients for our combined optical density/fluorescence (OD/F) device then are: Line 34: Line 36: * LED for optical density (for 600nm we recommend: DIALIGHT - 550-2505F ) * LED for optical density (for 600nm we recommend: DIALIGHT - 550-2505F ) * LED for fluorescence (any 450nm blue works for iLOV, 480nm for wtGFP ) * LED for fluorescence (any 450nm blue works for iLOV, 480nm for wtGFP ) + + The case is from acrylic glass. The construction plan can be downloaded from [2014.igem.org/Team:Aachen insert link here]. + == Fluorescence == == Fluorescence == + + Also general information + + === Characteristic Curve === == Optical Density == == Optical Density == + + Some general information + + === calibration === {{Team:Aachen/Footer}} {{Team:Aachen/Footer}}

# Open Source DIY Hardware

Being in the measurement track and having a team of highly motivated engineering and computer science students, we tackled the challenge to build, document and evaluate our open source hardware approach.

For our daily tasks in the lab, two key devices were detected: fluorometer and OD-meter. As we use GFP most of the time, the fluorometer is designed to work best with GFP. For modularity reasons, and re-usability, it is designed such that a change to another fluorescence protein is easy.

Besides the mandatory $\mu$-controller architecture, we worked together with the Fablab Aachen to construct the device. There we have the chance to use laser cutters and 3D printers.

The core component for detecting the light intensity is the cuvette holder. Please find the 3D model we printed below:

This cuvette holder can be used for both devices: the whole in the bottom is used for fluorescence measurement, the two opposite wholes are used for the light sensor and the LED for optical density measurement respectively.

### Hardware Requirements

The key ingredients for our combined optical density/fluorescence (OD/F) device then are:

• Arduino UNO R3 (or equivalent)
• bluetooth Modem optional
• TSL 235 R light to frequency sensor
• LED for optical density (for 600nm we recommend: DIALIGHT - 550-2505F )
• LED for fluorescence (any 450nm blue works for iLOV, 480nm for wtGFP )

The case is from acrylic glass. The construction plan can be downloaded from [2014.igem.org/Team:Aachen insert link here].

## Fluorescence

Also general information

## Optical Density

Some general information