http://2014.igem.org/wiki/index.php?title=Special:Contributions/Maschi&feed=atom&limit=50&target=Maschi&year=&month=2014.igem.org - User contributions [en]2024-03-29T01:35:03ZFrom 2014.igem.orgMediaWiki 1.16.5http://2014.igem.org/Team:Paris_Saclay/Ethics/Reflection_about_Artificial_FoodTeam:Paris Saclay/Ethics/Reflection about Artificial Food2014-10-18T03:46:44Z<p>Maschi: </p>
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<div>{{Team:Paris_Saclay/ethics_header}}<br />
<br/><br/><br/><br />
=Reflection about Artificial Food=<br />
==Introduction==<br />
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The human population strongly increased passing from 3 billion in 1960 to 7 billion in 2011, and forecasts predicted that population will reach about 9 billion people in 2050. Furthermore, increasing food production capacity is mandatory. However, in 2014, it took less than 8 months for Humanity to exhaust Earth's budget for the year and the food production has a strong impact on the environment such as deforestation, competition with humans for water, green house effects, land degradation etc… For example, to meet population’s demands in 2050, FAO reported that meat production has to double. In this context, how to feed everybody in a sustainable manner is thus a very important question. An example of these challenges is the livestock production. Indeed, livestock production accounts for 18% of anthropogenic greenhouse effect, 37% of anthropogenic methane, 65% of anthropogenic Nitrous Oxide and 64% of anthropogenic ammonia. It also account for 70% of all agricultural lands and for 8% of global humans water uses. By the time, 64% of the population is expected to live in water-stressed basins by 2025, there are still hundreds of millions people that lives undernourished [1]. In this context, would artificial food be the solution?<br />
<br />
==Why seek to produce artificial food?==<br />
<br />
Studies have shown that, in the case of artificial production of meat, growing it in-vitro would ensure a decrease of 96% of greenhouse effects emission and a decrease of 7 to 45% of energy. Furthermore, only 1% of agricultural lands and 4% of the water currently devoted to livestock would be enough to produce the same amount of meat [2]. Taken together, these results suggest that artificial food would decrease anthropogenic impact on environment such as deforestation, greenhouse effect and redirect feed crop production for humans. However, other studies points out several facts. Firstly, no one knows actually which process will finally be chosen to produce artificial meat so that it is hard to estimate the future impact of in-vitro meat to the environment. Secondly, part of the production process has not be accounted such as growth factors that are needed in the industrial process Thus, their environmental impact has not been yet evaluated. Thirdly, a big part of the livestock production which needed for milk production is used to produce meat (40% in France), suggesting that a big part of the livestock is still needed. Taken together, these results shows that the benefits of artificial meat are much less than one can expect [3]. However, in the case of meat production, the scientist consensus is that artificial food would ensure better living condition of livestock.<br />
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==How did we get to the point of producing artificial food ?==<br />
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Considering the depletion of Earth resources and the need to feed human population as well as taking care of the environment, it is interesting to look at the definition and the nature of the living. Indeed, what is life and its meaning? This is probably one of the most important questions for mankind, and many [https://2014.igem.org/Team:Paris_Saclay/Ethics/About_Life_Art_Science philosophers] and [https://2014.igem.org/Team:Paris_Saclay/Ethics/Scientific_Definition_of_Living-Being scientists] attempted to answer this question. Thus, one of the definitions of life is the role of each individual to perpetuate the species by reproduction. As life does not exist without death, it raises also the issue of population regulation. This can be achieved in many different ways. On the one hand there are intrinsic factors: some species are non-regulated, that multiply whatever its population density is. Conversely, in regulated species, the population size is strongly related to the growth per individual. On the other hand there are extrinsic factors, such as predators, weather, disease, etc. Thus, the population dynamics depend on both intrinsic and extrinsic factors. What could pathogenic bacteria and humans in common? Both can damage their respective host (a living organism for bacteria and Earth for humans). Both can show some similarities in their population curve: a lag phase and an exponential phase. In test tubes, bacteria can then reach a stationary phase, which means that bacteria consume all their media. This step is followed by population decrease (death). Concerning humans, what will happen? As Humanity consumes more than the Earth can provide, feed stock decrease. Thus, if humans reach a certain point in which earth resources do not sufficiently renew themselves to feed the entire population, human population would reach an equilibrium (number of births = number of deaths). Otherwise, humans could find a way to avoid it. For how long? Does the reproductive meaning of life inevitably tend towards auto-destruction?<br />
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[[File:Paris_Saclay_ethique-croissancebacterienne.jpg|350px|left]]<br />
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[[File:Paris_Saclay_ethique-croissancehumaine.jpg|350px|left]]<br />
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<br />
Indeed, Earth resource depletion is also the result of population way of life. It is known that the population of rich countries consume more food than they need and waste too much food. To develop this fact, a better understanding of the definition and the nature of the living is needed. <br />
According to Jean Paul Sartre, humans are the results of their experience: “You are nothing else but what you live. ... A man is no more than a series of undertakings [and] the sum of the organization that constitutes these undertakings". This aspect also shows that individual development is conditioned in regard of the society. Therefore, undertakings raises question of the origins of human actions.<br />
Thus, many philosophers describe selfishness as the source of human action. For Max Stirner, “Everyone is the center of his own world. World is only what he himself is not, but what belongs to him, is in a relationship with him, exists for him”. This definition of selfishness is a view where humans are always motivated by self-interest. Stephen Kendrick adds that “Almost every sinful action ever committed can be traced back to a selfish motive. It is a trait we hate in other people but justify in ourselves”. This means that even in when it seem to be acts of altruism, they do so because of the personal benefits that they themselves expect to obtain, directly or indirectly, from doing so. Furthermore, Max Stirner once said: “we have a single relationship with one another, that of usability, utility, use”. These examples emphasize the view of mutual benefits between two people motivated by their respective self-interests. Thus is selfishness, by centering interests on humans without taking account of the environment, one of the origins of the increasingly depletion of Earth resources? In the other hand, will selfishness resolve these environmental issues when it will be the human interest to do so? <br />
<br />
Ayn Rand makes a difference between humans and the other animals: "While animals survive by adjusting themselves to their background, man survives by adjusting his background to himself”. This means that the apparition of an extrinsic factor (climate change, disease …) often result in the decline of animals population whereas humans can accommodate. This concept relies on the differences between Man and animals. According to Ayn Rand, humans are “rational animals” (possess faculty of reason) whereas the other don’t.<br />
Thus, to accommodate to the feedstock decreasing, will we soon or late need to strongly regulate the number of birth as already does China? Otherwise, is artificial food the way by which humans will accommodate to earth resources depletion ?<br />
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==Would population accept artificial food ?==<br />
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It is an interesting question as it relies on the perception and conception of things and also depends in the social-cultural context we live in. <br />
<br />
[[File:Paris_Saclay_Treacheries.png|400px|center]]<br />
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Thus, the painter Magritte with its painting named “Ceci n’est pas une pipe”- “this is not a pipe” highlights this topic. This painting showing a pipe above the declaration “ceci n’est pas une pipe” is an example of naïve realism, which theory says that real objects exists as they are perceived. Thus, in the first place seeing this painting, one would say that “it’s a pipe”. However, “could you stuff the pipe?” would say Magritte. Then, one would realize that it is an image of a pipe. Thus, it would be a lie to write “this is a pipe” instead of “this is not a pipe”. Magritte further suggests that our compulsion to call the image a pipe reveals our predisposition to confuse the image with the thing it represents: the conception and perception of things are what they make them real to us. <br />
According to Hanni Rützler (nutritionist) “It is not the technology that would determine what we will eat tomorrow, but our collective consciousness”. Thus, what are the objections of the population in regard of artificial food? Studies showed three main factors. Firstly, the process of production itself: one can consider that artificial food is part of the continuous effort of humans to industrialize food production since centuries (GMOs, genetic selection of livestock …). Or is it a new manipulation of the nature for the benefits of humans? Secondly, the “disgust” of eating artificial food could be a strong psychological barrier. Thirdly, the danger about a product which effects on human health has not been assessed. <br />
Moreover, to the nutritionist Jean-Michel Lecerf, food is a combination of the necessary needs of our body with the consumer’s perception: «I think that we do not eat food in an independent manner of what it significate ». Thus, if people are feed by test-tubes, “why not just absorb a liquid of what our body needs (proteins, vitamins, carbohydrates…)?”. Here, Jean-Michel Lecerf assesses the loss of meaning. Artificial food could lead to the loss of the essence of food, of the worth of breeding (in the case of meat production) and the loss of what shape countries (agricultural fields). It is also interesting to note that artificial food goes in the opposite direction of the current movement that want people reapropriate what is in their plate (organic food, no GMOs …). <br />
Thus, considering these facts and a right communication and marketing about Fast lemon in the, would people accept it? Furthermore, as mentioned before, selfishness can be considered as a key factor in human choices. Thus, creating artificial food of better quality than the natural one would impact the acceptability of artificial food by the population?<br />
<br />
==What could be the impact of artificial food on the society ?==<br />
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One can imagine that the development of the production of artificial food will lead to a deep changes in the society. Some companies already work on producing artificial food in vitro or by 3D printing. However, if our food becomes only artificial food, our economic system will be impacted especially the agriculture domain. This question is not a current question as agricultural machine (tractor) have already replaced humans. But this hypothesis will be taken to the extreme. Indeed, if artificial food would feed the whole planet farmers would become useless. So can we think that the scientist would be the farmer of the 21th century? How can it impact on our society?<br />
The economic system would be shaken: increase of unemployment for farmers and agricultural companies, appearances of new professions, new market around artificial food. <br />
However, beneficial consequences may be also visible. Air and soil pollution may decrease and quality of life may improve especially in counties where pollution is currently a big issue. <br />
Finally, considering that this hypothesis would be possible, the whole society would be shaken. Life would be different for everyone, in every domain.<br />
<br />
==References==<br />
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[1] Livestock’s long shadows - environmental issues and option. 2006. Food and Agriculture Organization.<br />
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[2] http://www.maastrichtuniversity.nl/web/Main/Research/ResearchUM/FirsteverPublicTastingOfLabgrownCulturedBeefBurger.htm.<br />
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[3] J.F Hoquette et al. La viande du future sera-t-elle produite in vitro ? INRA Prod. Anim.,2013, 26 (4), 363-374<br />
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{{Team:Paris_Saclay/default_footer}}</div>Maschihttp://2014.igem.org/Team:Paris_Saclay/lemonpartyTeam:Paris Saclay/lemonparty2014-10-18T03:38:45Z<p>Maschi: Created page with "{{Team:Paris_Saclay/default_header}} =Lemon Party= Hmm... I know what you are looking for! {{Team:Paris_Saclay/default_footer}}"</p>
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<div>{{Team:Paris_Saclay/default_header}}<br />
=Lemon Party=<br />
Hmm... I know what you are looking for!<br />
{{Team:Paris_Saclay/default_footer}}</div>Maschihttp://2014.igem.org/Team:Paris_Saclay/Project/Lemon_ShapingTeam:Paris Saclay/Project/Lemon Shaping2014-10-18T03:37:25Z<p>Maschi: </p>
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<div>{{Team:Paris_Saclay/project_header}}<br />
=Lemon Shaping=<br />
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<br />
==Introduction==<br />
<br />
We decided to build up a lemon tree because it is traditionally accepted that trees embody development, evolution and life. This image sends us back to one of the first issues evoked in our project : defining life.<br />
Trees also allow us to figure out time and its impact on living things, season after season. In the case of lemon tree, even if leaf are evergreen, we can easily observe formation of buds, flowers and finally fruits. This theme of time’s impact on living organisms has been exploited in our wiki with the branch which is shown on top of the page: this branch grows as we progress in the wiki.<br />
The work we have done to make the lemon pass from green to yellow as well as the expression of perfumed molecules enable to mimic faithfully living things… brings us back to the following questions:<br />
* does synthetic biology blur the divide between living organisms and inert matter? As we create a sculpture partly composed of GMO and faithful to the idea we have of a lemon tree, we make people think that this sculpture is really a lemon tree…<br />
* do you think that bacteria, whose biological functions judged "useless" are deleted in order to maximize the production of a molecule, are closer to machines than living things?<br />
* some people think that this king of production can solve ecological problems linked to (big scale) farming: if this becomes reality, would you be ready to eat food produced via synthetic biology?<br />
<br />
Unfortunately, as transporting GMOs abroad or synthesizing them in Boston was impossible, we withdrew this idea.<br />
[[File:Paris Saclay IMG 3826.jpg|200px|right]]<br />
*structure:<br />
**trunk and branch: metal <br />
**leaf: silk paper with different green patterns<br />
**lemon: gel<br />
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We wanted to create our lemon on a base of agar gel, which can be a good medium for bacteria and cohesive enough to support its own structure. Our very first idea was to print the lemon with agar, but 3D printer are still pricey and the realization quite difficult given that we do not have much time allowed. We eventually opted for a more conventional way to achieve our objective: molding!<br />
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[[File:Paris Saclay lemon5.JPG|230px|left|]]<br />
[[File:Paris Saclay lemon2.jpg|230px|left|]]<br />
[[File:Paris Saclay lemon3.JPG|220px|left|]]<br />
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==Realisation==<br />
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First of all, we needed to know the optimal concentrations of agar to make our gel. Too much agar, and the bacteria colors would be masked by the natural one of agar, plus, air would not properly pass through and bacteria would die. Not enough agar, and the structure would collapse. Various [https://2014.igem.org/Team:Paris_Saclay/Notebook/August/5 tests] have been carried out and we conjectured that the most efficient gel for our purpose has a concentration of 25 mg/l.<br />
<br />
Secondly, we tried different [https://2014.igem.org/Team:Paris_Saclay/Notebook/August/12 concentrations] of bacteria to check if our gel would be translucent enough to see the color of our bacteria. The problem was the LB medium since it is naturally colored. We eventually opted for a colorless one : M63 medium.<br />
We also prepared bacteria expressing amplified [https://2014.igem.org/Team:Paris_Saclay/Notebook/August/29#Friday_29th_August Fluorescent Protein] in the case that we could not achieve our chromoprotein. <br />
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[[File:Photo agar test 002.jpg|230px|left|]]<br />
[[File:Paris Saclay moldsilicon.JPG|230px|left|]]<br />
[[File:Paris Saclay lemon3.JPG|180px|left|]]<br />
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<html><hr class="cleanhr"/></html><br />
<br />
Concerning the mold itself, we came up with an [https://2014.igem.org/Team:Paris_Saclay/Notebook/August/8 alternative solution to mold] using only agar (left picture) but we finally had two other options: a home-made silicone mold (picture at the center) and an other mold made by the [https://2014.igem.org/Team:INSA-Lyon Lyon] team using a 3D printer (right picture).<br />
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{{Team:Paris_Saclay/default_footer}}</div>Maschihttp://2014.igem.org/File:Paris_Saclay_IMG_3826.jpgFile:Paris Saclay IMG 3826.jpg2014-10-18T03:36:43Z<p>Maschi: </p>
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<div></div>Maschihttp://2014.igem.org/Template:Team:Paris_Saclay/default_headerTemplate:Team:Paris Saclay/default header2014-10-18T03:33:05Z<p>Maschi: </p>
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<li><a href="https://2014.igem.org/Team:Paris_Saclay/Project/Odor-free_ecoli">Remove the bad smell of E.coli</a></li><br />
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<li><a href="https://2014.igem.org/Team:Paris_Saclay/Modeling/bacterial_Growth">Bacterial Growth</a></li><br />
<li><a href="https://2014.igem.org/Team:Paris_Saclay/Modeling/Fusion_Proteine">Fusion Protein</a></li><br />
<li><a href="https://2014.igem.org/Team:Paris_Saclay/Modeling/odor">Odor</a></li><br />
</ul><br />
</li><br />
<li><a href="https://2014.igem.org/Team:Paris_Saclay/Labwork">Labwork</a><br />
<ul><br />
<li><a href="https://2014.igem.org/Team:Paris_Saclay/Notebook">Notebook</a></li><br />
<li><a href="https://2014.igem.org/Team:Paris_Saclay/Parts">Parts</a></li><br />
<li><a href="https://2014.igem.org/Team:Paris_Saclay/Protocols">Protocols</a></li><br />
<li><a href="https://2014.igem.org/Team:Paris_Saclay/Safety">Safety</a></li><br />
</ul><br />
</li><br />
<li><a href="https://2014.igem.org/Team:Paris_Saclay/Ethics">Ethics</a><br />
<ul><br />
<li><a href="https://2014.igem.org/Team:Paris_Saclay/Ethics/About_Life_Art_Science">Philosophical and Historical Aspects</a></li><br />
<li><a href="https://2014.igem.org/Team:Paris_Saclay/Ethics/Sociological_Cultural_Definition_Living_Being">Sociological and Cultural Aspects</a></li><br />
<li><a href="https://2014.igem.org/Team:Paris_Saclay/Ethics/Scientific Definition of Living-Being">Scientific Aspects</a></li><br />
<li><a href="https://2014.igem.org/Team:Paris_Saclay/Ethics/Interviews">Expert's Opinions</a></li><br />
<li><a href="https://2014.igem.org/Team:Paris_Saclay/Ethics/Round_Table">French iGEMers Discussion</a></li><br />
<li><a href="https://2014.igem.org/Team:Paris_Saclay/Ethics/Survey">International iGEMers Point-of-View</a></li><br />
<li><a href="https://2014.igem.org/Team:Paris_Saclay/Ethics/Reflection_about_Artificial_Food">Reflection about Artificial Food</a></li><br />
</ul><br />
</li><br />
<li><a href="https://2014.igem.org/Team:Paris_Saclay/Outreach">Outreach</a><br />
<ul><br />
<li><a href="https://2014.igem.org/Team:Paris_Saclay/Project/Workshop">French Meetup</a><br />
<li><a href="https://2014.igem.org/Team:Paris_Saclay/Outreach/Events/Curiositas">Curiositas</a><br />
<li><a href="https://2014.igem.org/Team:Paris_Saclay/Outreach/Events/Science_Festival">Science Festival</a><br />
<li><a href="https://2014.igem.org/Team:Paris_Saclay/Outreach/Collaborations">Collaborations</a></li><br />
</ul><br />
</li><br />
</ul><br />
</div><br />
<div id="pageContent"></html></div>Maschihttp://2014.igem.org/Team:Paris_Saclay/Project/Boston_InstallationTeam:Paris Saclay/Project/Boston Installation2014-10-18T03:21:09Z<p>Maschi: </p>
<hr />
<div>{{Team:Paris_Saclay/project_header}}<br />
=Boston Installation=<br />
The idea is to produce a sculpture, a branch, which would be in front of a mirror. On the spectator side, the branch seems « normal » but its reflection in the mirror shows something else : the reflection would reveal the inner branch, as a dissection. And we could see mechanisms as in a machine. The aim of this structure is to show the gap between a object and its reflection, between real things and decoys.<br />
<br />
[[File:Paris Saclay boston-installation.jpg|center]]<br />
<br />
{{Team:Paris_Saclay/default_footer}}</div>Maschihttp://2014.igem.org/File:Paris_Saclay_boston-installation.jpgFile:Paris Saclay boston-installation.jpg2014-10-18T03:20:28Z<p>Maschi: </p>
<hr />
<div></div>Maschihttp://2014.igem.org/Team:Paris_Saclay/Project/Lemon_ShapingTeam:Paris Saclay/Project/Lemon Shaping2014-10-18T02:41:06Z<p>Maschi: </p>
<hr />
<div>{{Team:Paris_Saclay/project_header}}<br />
=Lemon Shaping=<br />
<br />
<br />
==Introduction==<br />
<br />
We decided to build up a lemon tree because it is traditionally accepted that trees embody development, evolution and life. This image sends us back to one of the first issues evoked in our project : defining life.<br />
Trees also allow us to figure out time and its impact on living things, season after season. In the case of lemon tree, even if leaf are evergreen, we can easily observe formation of buds, flowers and finally fruits. This theme of time’s impact on living organisms has been exploited in our wiki with the branch which is shown on top of the page: this branch grows as we progress in the wiki.<br />
The work we have done to make the lemon pass from green to yellow as well as the expression of perfumed molecules enable to mimic faithfully living things… brings us back to the following questions:<br />
* does synthetic biology blur the divide between living organisms and inert matter? As we create a sculpture partly composed of GMO and faithful to the idea we have of a lemon tree, we make people think that this sculpture is really a lemon tree…<br />
* do you think that bacteria, whose biological functions judged "useless" are deleted in order to maximize the production of a molecule, are closer to machines than living things?<br />
* some people think that this king of production can solve ecological problems linked to (big scale) farming: if this becomes reality, would you be ready to eat food produced via synthetic biology?<br />
<br />
Unfortunately, as transporting GMOs abroad or synthesizing them in Boston was impossible, we withdrew this idea.<br />
<br />
structure:<br />
<br />
trunk and branch: metal <br />
<br />
leaf: silk paper with different green patterns<br />
<br />
lemon: gel<br />
We wanted to create our lemon on a base of agar gel, which can be a good medium for bacteria and cohesive enough to support its own structure. Our very first idea was to print the lemon with agar, but 3D printer are still pricey and the realization quite difficult given that we do not have much time allowed. We eventually opted for a more conventional way to achieve our objective: molding!<br />
<br />
[[File:Paris Saclay lemon5.JPG|230px|left|]]<br />
[[File:Paris Saclay lemon2.jpg|230px|left|]]<br />
<br />
==Realisation==<br />
<br />
First of all, we needed to know the optimal concentrations of agar to make our gel. Too much agar, and the bacteria colors would be masked by the natural one of agar, plus, air would not properly pass through and bacteria would die. Not enough agar, and the structure would collapse. Various [https://2014.igem.org/Team:Paris_Saclay/Notebook/August/5 tests] have been carried out and we conjectured that the most efficient gel for our purpose has a concentration of 25 mg/l.<br />
<br />
Secondly, we tried different [https://2014.igem.org/Team:Paris_Saclay/Notebook/August/12 concentrations] of bacteria to check if our gel would be translucent enough to see the color of our bacteria. The problem was the LB medium since it is naturally colored. We eventually opted for a colorless one : M63 medium.<br />
We also prepared bacteria expressing amplified [https://2014.igem.org/Team:Paris_Saclay/Notebook/August/29#Friday_29th_August Fluorescent Protein] in the case that we could not achieve our chromoprotein. <br />
<br />
[[File:Photo agar test 002.jpg|220px|left]]<br />
[[File:Paris Saclay moldsilicon.JPG|220px|left|]]<br />
[[File:Paris Saclay lemon3.JPG|220px|left|]]<br />
<br />
Concerning the mold itself, we came up with an [https://2014.igem.org/Team:Paris_Saclay/Notebook/August/8 alternative solution to mold] using only agar (left picture) but we finally had two other options: a home-made silicone mold (picture at the center) and an other mold made by the [https://2014.igem.org/Team:INSA-Lyon Lyon] team using a 3D printer (right picture).<br />
<br />
{{Team:Paris_Saclay/default_footer}}</div>Maschihttp://2014.igem.org/Team:Paris_Saclay/Project/Lemon_ShapingTeam:Paris Saclay/Project/Lemon Shaping2014-10-18T02:39:09Z<p>Maschi: /* Lemon Shaping */</p>
<hr />
<div>{{Team:Paris_Saclay/project_header}}<br />
=Lemon Shaping=<br />
<br />
<br />
==Introduction==<br />
<br />
We decided to build up a lemon tree because it is traditionally accepted that trees embody development, evolution and life. This image sends us back to one of the first issues evoked in our project : defining life.<br />
Trees also allow us to figure out time and its impact on living things, season after season. In the case of lemon tree, even if leaf are evergreen, we can easily observe formation of buds, flowers and finally fruits. This theme of time’s impact on living organisms has been exploited in our wiki with the branch which is shown on top of the page: this branch grows as we progress in the wiki.<br />
The work we have done to make the lemon pass from green to yellow as well as the expression of perfumed molecules enable to mimic faithfully living things… brings us back to the following questions:<br />
* does synthetic biology blur the divide between living organisms and inert matter? As we create a sculpture partly composed of GMO and faithful to the idea we have of a lemon tree, we make people think that this sculpture is really a lemon tree…<br />
* do you think that bacteria, whose biological functions judged "useless" are deleted in order to maximize the production of a molecule, are closer to machines than living things?<br />
* some people think that this king of production can solve ecological problems linked to (big scale) farming: if this becomes reality, would you be ready to eat food produced via synthetic biology?<br />
<br />
Unfortunately, as transporting GMOs abroad or synthesizing them in Boston was impossible, we withdrew this idea.<br />
<br />
structure:<br />
<br />
trunk and branch: metal <br />
<br />
leaf: silk paper with different green patterns<br />
<br />
lemon: gel<br />
We wanted to create our lemon on a base of agar gel, which can be a good medium for bacteria and cohesive enough to support its own structure. Our very first idea was to print the lemon with agar, but 3D printer are still pricey and the realization quite difficult given that we do not have much time allowed. We eventually opted for a more conventional way to achieve our objective: molding!<br />
<br />
[[File:Paris Saclay lemon5.JPG|230px|left|]]<br />
[[File:Paris Saclay lemon2.jpg|230px|left|]]<br />
<br />
==Realisation==<br />
<br />
First of all, we needed to know the optimal concentrations of agar to make our gel. Too much agar, and the bacteria colors would be masked by the natural one of agar, plus, air would not properly pass through and bacteria would die. Not enough agar, and the structure would collapse. Various [https://2014.igem.org/Team:Paris_Saclay/Notebook/August/5 tests] have been carried out and we conjectured that the most efficient gel for our purpose has a concentration of 25 mg/l.<br />
<br />
Secondly, we tried different [https://2014.igem.org/Team:Paris_Saclay/Notebook/August/12 concentrations] of bacteria to check if our gel would be translucent enough to see the color of our bacteria. The problem was the LB medium since it is naturally colored. We eventually opted for a colorless one : M63 medium.<br />
We also prepared bacteria expressing amplified [https://2014.igem.org/Team:Paris_Saclay/Notebook/August/29#Friday_29th_August Fluorescent Protein] in the case that we could not achieve our chromoprotein. <br />
<br />
Concerning the mold itself, we came up with an [https://2014.igem.org/Team:Paris_Saclay/Notebook/August/8 alternative solution to mold] using only agar (left picture) but we finally had two other options: a home-made silicone mold (picture at the center) and an other mold made by the [https://2014.igem.org/Team:INSA-Lyon Lyon] team using a 3D printer (right picture).<br />
<br />
[[File:Photo agar test 002.jpg|220px|left]]<br />
[[File:Paris Saclay moldsilicon.JPG|220px|left|]] <br />
[[File:Paris Saclay lemon3.JPG|220px|left|]]<br />
<br />
{{Team:Paris_Saclay/default_footer}}</div>Maschihttp://2014.igem.org/Team:Paris_Saclay/Project/FastlemonTeam:Paris Saclay/Project/Fastlemon2014-10-18T02:32:32Z<p>Maschi: </p>
<hr />
<div>{{Team:Paris_Saclay/project_header}}<br />
<br />
=<FONT color="F68127">FastLemon</FONT>=<br />
<br />
<FONT face="arial"><FONT color="956A12"><FONT size="5pt">'''Have you ever dreamt about having your food magically appeared ?'''<br />
<br />
'''Without going to the supermarket? Without cooking for hours?'''</FONT></FONT><br />
<br />
<FONT size="5pt"><center>'''<FONT color="F68127">Now your dreams come true because YOU have <U>FastLemon</U></font></center></FONT><br />
<br />
<html><div style="width:560px; margin:0 auto;"><iframe width="560" height="315" src="//www.youtube.com/embed/8ayVrNLyxTU" frameborder="0" allowfullscreen></iframe></div></html><br />
<br />
===I bet you're wondering, what is FastLemon?===<br />
<br />
'''FastLemon is the better way to have your food in a flash.''' '''It's like a lemon: the smell is the same, the color is the same and the taste is the same.'''<br />
'''Like a lemon, made by cells linked together by protein, sugar, lipids and others, FASTLEMON contains some ''E. coli'' joined together by agar. So you have exactly the same: cells and sugar. FastLemon is as living as a lemon !'''<br />
<br />
'''After a hard day FASTLEMON is always welcome!'''<br />
<br />
<html><div style="width:560px; margin:0 auto;"><iframe width="420" height="315" src="//www.youtube.com/embed/wQ7rJocIirE" frameborder="0" allowfullscreen></iframe></div></html><br />
<br />
'''This will brighten up your day.'''<br />
<br />
===Why?===<br />
<br />
'''Because our FastLemon has such a wonderful fragrance that you will forget all your problems.'''<br />
<br />
'''In effect FASTLEMON has particular properties that make it very attractive:'''<br />
*'''The bad ''E.coli'' smell was [https://2014.igem.org/Team:Paris_Saclay/Project/Odor-free_ecoli removed]''' <br />
*'''The lemon scent is so captivating because the ''E.coli'' smell was [https://2014.igem.org/Team:Paris_Saclay/Project/Lemon_Scent modified]'''<br />
*'''The apparence is like a lemon: same [https://2014.igem.org/Team:Paris_Saclay/Project/Salicylate_Inducible_System ripening] and same [https://2014.igem.org/Team:Paris_Saclay/Project/Lemon_Shaping shape].'''<br />
<br />
'''So that's why you have to choose FASTLEMON as many consumers already have:'''<br />
<br />
<html><div style="width:560px; margin:0 auto;"><iframe width="560" height="315" src="//www.youtube.com/embed/kITqpbNPgqA" frameborder="0" allowfullscreen></iframe></div></html><br />
<br />
===They tried it!===<br />
<br />
'''Juliette : "''Since I tried FASTLEMON, I can't spend a single day without FASTLEMON''"'''<br />
<br />
'''Leila : "''Since I tried FASTLEMON I have finally time for myself''"'''<br />
<br />
'''Terry : "''Since I tried FASTLEMON my wife cooks better than before''"'''<br />
<br />
'''Romain: "''I tried FASTLEMON the day's end is much more colorful''"'''<br />
<br />
<div style="border: 3px solid" class="center"><br/><FONT size="4pt">'''ARE YOU READY TO EAT FASTLEMON ?'''</FONT><br />
<br />
'''Do you think we can believe this advertisement? In your opinion, is it a real or fake lemon?''' '''Living or not living?'''<br />
<br />
'''Does the ad succeed in convincing you to try the product?''' '''Is there any limit with biology?'''<br />
<br />
'''Here are some of the questions we raised in our project.''' [https://2014.igem.org/Team:Paris_Saclay/Ethics/Reflection_about_Artificial_Food Click to see more] !<br/><br/></div><br />
<br />
{{Team:Paris_Saclay/default_footer}}</div>Maschihttp://2014.igem.org/Team:Paris_SaclayTeam:Paris Saclay2014-10-18T02:11:11Z<p>Maschi: /* We let you discover our FastLemon */</p>
<hr />
<div>{{Team:Paris_Saclay/home_header}}<br />
===Project Abstract: This is not a lemon!===<br />
[[File:Paris_Saclay_university.png|200px|right|link=http://www.u-psud.fr/]]<br />
<html><div id="englishversion"></html><br />
Synthetic Biology has the power of potentially changing paradigms of society such as our conception of what living beings are. If we deprive bacteria from all their “unnecessary" functions and have them produce what we need and desire, do these bacteria still possess the status of living organisms or have they become machines?<br />
To raise and explore this question, we adopted an artistic approach. Indeed, we think that '''Bio-Art''' is one of the best ways to reach the citizens and to spark debate and reflection.<br />
<br />
We decided to create a concept organism that would reflect these interrogations. We plan to modify ''Escherichia coli'' in order to produce the '''fragrance''' of a lemon and simulate the '''ripening''' process of a lemon by changing its color gradually from green to yellow. A mixture of bacteria and solid growth medium will be moulded in a lemon '''shape''': it will smell like a lemon, ripe like a lemon and look like a lemon, but "ceci n’est pas un citron” - this is not a lemon… or is it?<br />
<br />
With this project we invite everyone to think about the upcoming opportunities and the necessary ethical limits of designing living beings.<br />
<br />
When iGEM Paris Saclay gives you lemons, break your taboos!<br />
<html></div></html><br />
<html><div id="portugueseversion" class="invisible"></html><br />
A Biologia Sintética tem o poder de mudar os paradigmas da sociedade, como a nossa concepção do que são serem vivos. Se privarmos uma bactéria de todas as suas funções "desnecessárias" e a fizermos produzir o que precisamos e queremos, essa bactéria continuará com um status de organismo vivo ou ela tende a ser uma máquina? Para levantar e explorar essa questão, adotamos uma abordagem artística. De fato, pensamos que '''Bio-Arte''' é uma das melhores maneiras de atingir o grande público e estimar o debate.<br />
<br />
Nós decidimos criar um organismo conceito que refletiria essas interrogações. Nós planejamos modificar ''Escherichia coli'' para que essa produza o '''aroma''' e simule o amadurecimento de um limão, mudando gradualmente sua cor do verde para o amarelo. A mistura de bactérias e meio de cultivo serão moldados em uma forma de limão: ele vai cheirar como um limão, '''amadurecer''' como um limão e ter a '''forma''' de um limão, mas "ceci n'est pas un citron" - isto não é um limão... ou é? <br />
Assim, convidamos todos a pensar sobre as perspectivas e limites éticos de projetas seres vivos.<br />
<br />
Quando iGEM Paris-Saclay lhe der limões, quebre seus tabus!<br />
<br />
<br />
''This abstract was translated to Portuguese by [https://2014.igem.org/Team:Brasil-SP iGEM Brasil-SP] under our [https://2014.igem.org/Team:Paris_Saclay/Outreach/Collaborations collaborations].''<br />
<html></div></html><br />
<html><div id="chineseversion" class="invisible"></html><br />
合成生物学有着改变社会的能力,举个例子,所谓的改变社会,就是我们对于地球上生物的认知。<br />
<br />
如果我们删除了细菌中我们认为不重要的功能,又让他们做我们想让他们做的事情,那么这些细菌是否还保持正常生物体的状态呢?它们是否已经成为了一种生产机器了呢?为了解决这个问题,我们提出了一种艺术性的方法。众所周知,要想让我们的观念能够被大众更好地理解,生物艺术无疑是一种很好的方法。<br />
<br />
我们创建了一种机制能够解决这个问题。我们通过修饰大肠杆菌让它产生一种气味并且模仿柠檬成熟的过程,这个模型需要细菌和它赖以生存的培养基。我们通过五官的感知使这个模型看起来像一个柠檬,但是"ceci n’est pas un citron”——但事实上它并不是一个柠檬,我们只是让它看起来比较像而已。我们希望通过这个实例能够引发大家对生物实验的道德底线的思考。<br />
<br />
<br />
''This abstract was translated to Chinese by [https://2014.igem.org/Team:XMU-China iGEM XMU China] under our [https://2014.igem.org/Team:Paris_Saclay/Outreach/Collaborations collaborations].''<br />
<html></div></html><br />
<html><div id="finnishversion" class="invisible"></html><br />
Synteettisellä biologialla on mahdollisuus muuttaa yhteiskunnan paradigmoja, kuten käsitystämme soluista.<br />
<br />
Jos karsimme bakteereista niiden “tarpeettomat” toiminnot ja valjastamme ne vastaamaan tarpeisiimme, ovatko bakteerit edelleen mielestämme eläviä eliöitä vai onko niistä tullut koneita? Kysymme tämän kysymyksen biotaiteen keinoin, sillä se on yksi parhaita tapoja herättää julkista keskustelua.<br />
<br />
Me luommekonseptiorganismin, joka heijastaa tätä keskustelua. Me muuntelemme ''Escherichia coli'' bakteeria niin, että se tuoksuu ja jäljittelee sitruunan kypsymistä. Teemme bakteerien ja kasvulaustan sekoituksen, joka tuoksuu, kypsyy ja näyttää aivan sitruunalta, mutta "ceci n’est pas un citron” se ei ole sitruuna… vai onko? Tämän avulla haluamme herättää kaikissa ajatuksia elävien organismien suunnitteluun liittyvistä tulevaisuuden mahdollisuuksista ja eettisistä rajoitteista.<br />
<br />
<br />
''This abstract was translated to Finnish by [https://2014.igem.org/Team:Aalto-Helsinki iGEM Aalto-Helsinki] under our [https://2014.igem.org/Team:Paris_Saclay/Outreach/Collaborations collaborations].''<br />
<html></div></html><br />
<html><div id="frenchversion" class="invisible"></html><br />
La biologie synthétique a le pouvoir de changer certains paradigmes de notre société tel que notre conception du statut des êtres vivants.<br />
<br />
Si nous privons des bactéries de leurs fonctions "inutiles" pour qu'elles produisent ce dont nous avons besoin, peut-on toujours les considérer comme des organismes vivants ou sont-elles devenues des machines ?<br />
<br />
Pour aborder cette question, nous avons adopté une approche artistique car nous pensons que le Bio-Art est l'un des meilleurs moyens de susciter des débats avec les citoyens. Nous avons créé un organisme-concept qui pourrait refléter ces interrogations : nous avons modifié ''Escherichia coli'' pour qu'elle produise une odeur de citron et aussi simuler son processus de maturation. Un mélange de bactéries et de milieu de croissance sera coulé dans un moule qui sentira, mûrira et ressemblera à un citron. Mais "ceci n'est pas un citron"- ou cela l'est-il ? Avec notre réalisation nous lançons une invitation à la réflexion sur les futures opportunités et limites éthiques de la conception d’êtres vivants.<br />
<html></div></html><br />
<html><div id="italianversion" class="invisible"></html><br />
La biologia sintetica potenzialmente ha il potere di cambiare i paradigmi della società come la nostra concezione di ciò che gli esseri viventi sono. Se priviamo i batteri di tutte le loro funzioni "inutili" e facciamo produrre loro ciò di cui abbiamo bisogno e desiderio, questi batteri possiedono ancora lo status di organismi viventi o sono diventati macchine? Per aumentare e esplorare questa domanda, abbiamo adottato un approccio artistico. Infatti, pensiamo che il '''Bio-Art''' è uno dei modi migliori per raggiungere i cittadini e stimolare il dibattito e la riflessione. <br />
<br />
Abbiamo deciso di creare un organismo ideale che riflettesse tali interrogatori. Abbiamo in programma di modificare ''Escherichia coli'' per produrre il '''profumo''' di un limone e simulare il processo di maturazione di un limone cambiando il suo colore gradualmente dal verde al giallo. Una miscela di batteri e crescita solida si modellerà in una '''forma''' di limone: Odorerà come un limone, maturerà come un limone e sembrerà come un limone, ma "ceci n'est pas un citron" - questo non sarà un limone ... o lo è? <br />
<br />
Con questo progetto, invitiamo tutti a pensare alle prossime opportunità e ai limiti etici necessari di progettazione di esseri viventi. <br />
<br />
Quando iGEM Paris Saclay ti da limoni, rompete i vostri tabù!<br />
<html></div></html><br />
<html><div id="koreanversion" class="invisible"></html><br />
사물의 인식 및 개념은 우리가 사는 사회와 문화코드에 상당한 영향을 받습니다. 예를 들어, 옛날에는 어떤 인간은 다른 이의 소유물이었으며 그들의 생사는 주인이 결정하였습니다. 오늘날 이것이 도저히 용납이 안된다면 그것은 ‘인간’의 정의가 그동안 많이 바뀌었기 때문입니다.<br />
합성생물학은 사회의 패러다임을 바꿀 힘을 갖고 있습니다. ‘생물이란 무엇인가?’에 대한 새로운 답도 제시할 수 있습니다. 만약 박테리아에서 ‘필요없는’ 기능들을 제거하고 우리가 원하는 물질을 만들도록 하면, 이 박테리아는 아직도 살아있을까요? 아니면, 하나의 기계로 전락했을까요? 이 질문에 답하기 위해 저희는 예술의 힘을 빌리고자 합니다. 저희는 바이오아트 (BioArt)가 대중에게 다가가고 토론을 싹틔우기에 가장 좋은 방법이라 생각합니다.<br />
이런 생각들을 반영할만한 생명체를 만들고자 합니다. 대장균을 개조하여 레몬의 향과 색을 표현하는 것입니다. 특히, 과일이 익듯이 저희 대장균도 초록색에서 노란색으로 바뀔 것입니다. 레몬 모양의 틀을 이용하여 박테리아가 들어있는 배지도 레몬처럼 보이게 할 계획입니다. 결국 레몬향이 나고 레몬색도 띄고 레몬처럼 보이지만, “ceci n’est pas un citon” – 이것은 레몬이 아닙니다. 그런데 모든점이 레몬과 같다면, 정말 레몬이 아닐까요? <br />
저희 작품을 통해, 합성생물학의 윤리적인 한계에 대해서 다같이 생각해봅시다.<br />
iGEM Paris Saclay와 함께 당신의 터부를 버리세요!<br />
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Synthetische biologie heft het vermogen om de visie van de maatschappij te veranderen zoals on beeld van wat levende wezens zijn.<br />
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Als wij bacterieën ontdoen van hun ‘onnodige’ functies en ze laten produceren wat wij nodig hebben, hebben deze bacteriën dan nog de status van levende organismen, of zijn het machines geworden? Wij hebben een artistieke methode gekozen deze vraag op te werpen omdat Bio-Art de beste manier is om burgers met elkaar in debat te laten gaan.<br />
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We hebben een concept organisme gemaakt dat deze vragen weerspiegelt. We modificeren Escheria coli om de geur van een rijpende citroen te produceren en het rijpingsproces te simuleren. We gieten een mix van bacteriën en groeimedium in een vorm: Zo zal het ruiken, rijpen en er uit zien als een citroen, maar “ceci n’est pas un citron” – dit is geen citroen… Of toch wel? Hiermee nodigen we iedereen uit om na te denken over toekomstige kansen en de ethische grenzen van het ontwerpen van levende wezens.<br />
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''This abstract was translated to Dutch by [https://2014.igem.org/Team:Groningen iGEM Groningen] under our [https://2014.igem.org/Team:Paris_Saclay/Outreach/Collaborations collaborations].''<br />
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Biologia syntetyczna ma moc zmieniania paradygmatów społecznych takich jak nasza koncepcja tego, czym są organizmy żywe. Jeśli pozbawimy bakterie ich „niepotrzebnych” funkcji i zmusimy je do produkcji tego, co nam potrzebne, czy nadal będą one posiadały status organizmów żywych czy staną się maszynami?<br />
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Aby odpowiedzieć na to pytanie, przyjęliśmy podejście artystyczne, jako że Bio-Art jest jednym z najlepszych sposobów wskrzeszenia debaty wśród społeczeństwa. Stworzyliśmy koncepcję organizmu, która odzwierciedlałaby te dochodzenia. Zmodyfikowaliśmy Escherichia coli by wytwarzała zapach i imitowała proces dojrzewania cytryny. Mieszanina bakterii i pożywki odżywczej zostanie ukształtowana: będzie pachnieć, dojrzewać i wyglądać jak cytryna, ale "ceci n’est pas un citron” – to nie cytryna… a może jednak? Wraz z tym pytaniem zapraszamy wszystkich do rozważenia przyszłych okazji i granic etycznych projektowania żywych istot.<br />
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''This abstract was translated to Polish by [https://2014.igem.org/Team:Warsaw iGEM Warsaw] under our [https://2014.igem.org/Team:Paris_Saclay/Outreach/Collaborations collaborations].''<br />
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Syntetisk biologi er en mulighet til et paradigmeskifte i samfunnet med tanke på vår oppfatning av hva levende ting er. Hvis vi tar bort bakteriers ”unødvendige” funksjoner og får dem til å produsere ting som vi trenger, vil disse bakteriene fremdeles betraktes som levende organismer, eller har de blitt maskiner? For å sette fokus på denne problemstillingen har vi valgt en kunstnerisk tilnærming ettersom Bio-Kunst er en av de beste måtene å sette i gang en samfunnsdebatt på. Vi har skapt en konsept organisme som reflekterer denne problemstillingen. Vi har modifisert E. coli-bakterier til å produsere lukt og etterlikne modningsprosessen til en sitron. En blanding av bakterier og vekstmedium blandes sammen: Det vil lukte som, modne som og se ut som, en sitron, men ”Ceci n’est pas un citron” – dette er ikke en sitron… eller er det?<br />
Med dette inviterer vi alle til å tenke over hvilke muligheter og etiske grenser vi har når vi designer levende skapninger.<br />
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''This abstract was translated to Norwegian by [https://2014.igem.org/Team:UiOslo%20Norway iGEM UiOslo_Norway] under our [https://2014.igem.org/Team:Paris_Saclay/Outreach/Collaborations collaborations].''<br />
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<html><div id="spanishversion" class="invisible"></html><br />
La biología sintética tiene el poder de cambiar los paradigmas de la sociedad como nuestra concepción de la definición del ser viviente. <br />
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Si privamos las bacterias de todas sus funciones “inútiles” para que produzcan lo que necesitamos, ¿es que todavía poseen el estatuto de ser vivo o se convirtieron en máquinas?<br />
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Para plantear esta cuestión, adoptamos un enfoque artístico porque creemos que el Bio-Arte es una de las mejores maneras para sensibilizar la población y provocar el debate. Hemos creado un organismo-concepto que podría plantear estas interrogaciones: hemos modificado ''Escherichia coli'' para que produzca el olor del limón y simula su proceso de maturación . Una mezcla de bacterias y de medio de crecimiento sólido será moldeada en un molde de limón: el “limón” olerá como a limón, madurará como un limón y se parecerá a un limón pero “ceci n’est pas un citron” – no es un limón… ¿o lo es?.<br />
Con este proyecto, invitamos a cada uno a pensar acerca de las oportunidades futuras y la necesidad de los límites éticos con respecto al diseño de seres vivos. <br />
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Sentetik biyoloji, yaşayan canlılarla ve bizim açımızla toplum paradigmalarını değiştirme gücüne sahiptir.<br />
Eğer bizler bakterileri “gereksiz” fonksiyonlarından kurtarır ve kendi ihtiyaç duyduğumuz fonksiyonlar eklersek, bu bakteriler hala yaşayan canlı özelliklerine mi sahip olurlar yoksa birer makinaya mı dönüşürler?<br />
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Bu soruyu irdelemek için, Bio-Art adında bir yaklaşım geliştirdik. Dizaynımızın bu sorunu toplumla tartışmak için iyi bir yol olduğunu düşünüyoruz. Bu sorgulamaları yansıtacak bir örnek organizma yarattık. Parfüm üretmek ve bir limonun olgunlaşma sürecini uyarmak için Escherchia coli’yi modifiye ettik. Bir grup bakteri kültürü limon gibi olgunlaşacak, kokacak ve görünecek; ancak “ceci n’est pas un citron”-bu bir limon değil.. ya da öyle mi?<br />
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Bu projeyle, herkesi yaklaşan fırsatlar ve canlıların etik tasarımının sınırları hakkında düşünmeye davet ediyoruz.<br />
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''This abstract was translated to Turkish by [https://2014.igem.org/Team:METU%20Turkey iGEM METU_Turkey] under our [https://2014.igem.org/Team:Paris_Saclay/Outreach/Collaborations collaborations].''<br />
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<li><a href="https://2014.igem.org/Team:Paris_Saclay">Home</a></li><br />
<li><a href="https://2014.igem.org/Team:Paris_Saclay/Team">Team</a><br />
<ul><br />
<li><a href="https://igem.org/Team.cgi?year=2014&team_name=Paris_Saclay">Official Profile</a></li><br />
<li><a href="https://2014.igem.org/Team:Paris_Saclay/Attributions">Attributions</a></li><br />
<li><a href="https://2014.igem.org/Team:Paris_Saclay/Team/Sponsor">Sponsors</a></li><br />
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<li><a href="https://2014.igem.org/Team:Paris_Saclay/Project">Project</a><br />
<ul><br />
<li><a href="https://2014.igem.org/Team:Paris_Saclay/Project/Inspirations">Inspirations</a></li><br />
<li><a href="https://2014.igem.org/Team:Paris_Saclay/Project/Fastlemon">Fastlemon</a></li><br />
<li><a href="https://2014.igem.org/Team:Paris_Saclay/Project/Odor-free_ecoli">Remove the bad smell of E.coli</a></li><br />
<li><a href="https://2014.igem.org/Team:Paris_Saclay/Project/Lemon_Scent">Lemon Scent</a></li><br />
<li><a href="https://2014.igem.org/Team:Paris_Saclay/Project/Salicylate_Inducible_System">Lemon Ripening</a></li><br />
<li><a href="https://2014.igem.org/Team:Paris_Saclay/Project/Lemon_Shaping">Lemon Shaping</a></li><br />
<li><a href="https://2014.igem.org/Team:Paris_Saclay/Project/Boston_Installation">Boston Installation</a></li><br />
</ul><br />
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<li><a href="https://2014.igem.org/Team:Paris_Saclay/Modeling">Modeling</a><br />
<ul><br />
<li><a href="https://2014.igem.org/Team:Paris_Saclay/Modeling/oxygen_diffusion">Oxygen Diffusion</a></li><br />
<li><a href="https://2014.igem.org/Team:Paris_Saclay/Modeling/bacterial_Growth">Bacterial Growth</a></li><br />
<li><a href="https://2014.igem.org/Team:Paris_Saclay/Modeling/Fusion_Proteine">Fusion Protein</a></li><br />
<li><a href="https://2014.igem.org/Team:Paris_Saclay/Modeling/odor">Odor</a></li><br />
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<li><a href="https://2014.igem.org/Team:Paris_Saclay/Labwork">Labwork</a><br />
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<li><a href="https://2014.igem.org/Team:Paris_Saclay/Notebook">Notebook</a></li><br />
<li><a href="https://2014.igem.org/Team:Paris_Saclay/Parts">Parts</a></li><br />
<li><a href="https://2014.igem.org/Team:Paris_Saclay/Protocols">Protocols</a></li><br />
<li><a href="https://2014.igem.org/Team:Paris_Saclay/Safety">Safety</a></li><br />
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<li><a href="https://2014.igem.org/Team:Paris_Saclay/Ethics">Ethics</a><br />
<ul><br />
<li><a href="https://2014.igem.org/Team:Paris_Saclay/Ethics/About_Life_Art_Science">Philosophical and Historical Aspects</a></li><br />
<li><a href="https://2014.igem.org/Team:Paris_Saclay/Ethics/Sociological_Cultural_Definition_Living_Being">Sociological and Cultural Aspects</a></li><br />
<li><a href="https://2014.igem.org/Team:Paris_Saclay/Ethics/Scientific Definition of Living-Being">Scientific Aspects</a></li><br />
<li><a href="https://2014.igem.org/Team:Paris_Saclay/Ethics/Interviews">Expert's Opinions</a></li><br />
<li><a href="https://2014.igem.org/Team:Paris_Saclay/Ethics/Round_Table">French iGEMers Discussion</a></li><br />
<li><a href="https://2014.igem.org/Team:Paris_Saclay/Ethics/Survey">International iGEMers Point-of-View</a></li><br />
<li><a href="https://2014.igem.org/Team:Paris_Saclay/Ethics/Reflection_about_Artificial_Food">Reflection about Artificial Food</a></li><br />
</ul><br />
</li><br />
<li><a href="https://2014.igem.org/Team:Paris_Saclay/Outreach">Outreach</a><br />
<ul><br />
<li><a href="https://2014.igem.org/Team:Paris_Saclay/Project/Workshop">French Meetup</a><br />
<li><a href="https://2014.igem.org/Team:Paris_Saclay/Outreach/Events/Curiositas">Curiositas</a><br />
<li><a href="https://2014.igem.org/Team:Paris_Saclay/Outreach/Events/Science_Festival">Science Festival</a><br />
<li><a href="https://2014.igem.org/Team:Paris_Saclay/Outreach/Collaborations">Collaborations</a></li><br />
</ul><br />
</li><br />
</ul><br />
</div><br />
<div id="pageContent"></html></div>Maschihttp://2014.igem.org/Team:Paris_Saclay/LabworkTeam:Paris Saclay/Labwork2014-10-18T02:07:53Z<p>Maschi: </p>
<hr />
<div>{{Team:Paris_Saclay/labwork_header}}<br />
=Labwork=<br />
==Planning==<br />
We started the labwork in June. The first step was the research project scope and bibliography to ensure the feasibility of our project and to plan all the work we had to do during this wonderful iGEM summer. <br />
In early July we began the experiments, which was completed in the end of September. In order to be effective, we organized ourselves into four groups of summer interns. The groups were formed by all the members of the team, so even students from areas other than biology could cooperate for the project in the laboratory. To ensure the smooth development of the project by all students, the work had a life cycle : <br />
<br />
* Learning: A non biologist had to first learn the basics of biology to understand the lab work.<br />
* Practice: The novice starts to do their experiments with the help of another biology student or an adviser.<br />
* Development: The aforementioned student becomes autonomous and takes initiative.<br />
* Transmission of acquired knowledge: The student, initially oblivious to practices in the lab, can transmit this knowledge at the end of their internship to newcomers.<br />
<br />
===The ''E. coli'' odor-free chassis===<br />
*Culture of MG1655 and MG1655Z1 strains- [https://2014.igem.org/Team:Paris_Saclay/Notebook/June/30 30st July]<br />
*P1 phage stock preparation for the transduction of the ''Delta-tnaA::Kan'' - [https://2014.igem.org/Team:Paris_Saclay/Notebook/July/2 2nd July]<br />
*P1 phage transduction using the stock prepared on July 2nd to MG1655 and MG1655Z1 strains - [https://2014.igem.org/Team:Paris_Saclay/Notebook/July/3 3rd July]<br />
*Cultures of MG1655 and MG1655Z1 transductants - [https://2014.igem.org/Team:Paris_Saclay/Notebook/July/4 4st July]<br />
*Transformations test of competent cells MG1655 and MG1655Z1 - [https://2014.igem.org/Team:Paris_Saclay/Notebook/July/10 10th July]<br />
*Preparation of competent cells E coli, deletion of the antibiotic cassette in the odorless bacteria - [https://2014.igem.org/Team:Paris_Saclay/Notebook/July/11 11th July]<br />
*Preparation of competent cells E. coli MG1655 and MG1655Z1 transductants - [https://2014.igem.org/Team:Paris_Saclay/Notebook/July/17 17th July]<br />
*Transformation of supercompetent cells MG1655 and MG1655Z1 transductants with plasmid BT340- [https://2014.igem.org/Team:Paris_Saclay/Notebook/July/18 18th July]<br />
*Results of the transformation - [https://2014.igem.org/Team:Paris_Saclay/Notebook/July/21 21st July]<br />
*Preparation and transformation of competent MG1655 and MG1655Z1 transductants - [https://2014.igem.org/Team:Paris_Saclay/Notebook/July/22 22nd July]<br />
*PCR verification of the strains grown - [https://2014.igem.org/Team:Paris_Saclay/Notebook/July/25 25th July]<br />
*Preparation and Transformation of competent MG1655 and MG1655Z1 with BT340 - [https://2014.igem.org/Team:Paris_Saclay/Notebook/July/28 28th July]<br />
*PCR of MG1655 and MG1655Z1 with FTR-Apra-F and FTR-Apra-R - [https://2014.igem.org/Team:Paris_Saclay/Notebook/July/29 29th July]<br />
*Preparation and transformation of electrocompetent MG1655 and MG1655Z1 with plasmid BT340 - [https://2014.igem.org/Team:Paris_Saclay/Notebook/July/30 30st July]<br />
*Clone isolation by streak of ''Delta-tnaA'' MG1655 and MG1655Z1 on LB dishes - [https://2014.igem.org/Team:Paris_Saclay/Notebook/August/1 1st August]<br />
*Clone isolation by streak of ''Delta-tnaA'' MG1655 and MG1655Z1 on LB and kan dishes - [https://2014.igem.org/Team:Paris_Saclay/Notebook/August/4 4th August]<br />
*PCR verification of the final strains ''Delta-tnaA'' MG1655 and MG1655Z1 - [https://2014.igem.org/Team:Paris_Saclay/Notebook/August/5 5th August]<br />
*Final stock of MG1655 ''Delta-tnaA'' - [https://2014.igem.org/Team:Paris_Saclay/Notebook/August/6 6st August]<br />
<br />
===The Lemon Scent===<br />
====Preparation====<br />
* Rehydration of BioBricks BBa_J45014, BBa_K517003 - [https://2014.igem.org/Team:Paris_Saclay/Notebook/June/30 30th June]<br />
* Preparation of electrocompetent DY330 and transformation via pJBEI-6409 - [https://2014.igem.org/Team:Paris_Saclay/Notebook/July/18#Preparation_of_electrocompetent_DY330_and_transformation_via_pJBEI6409 18th July]<br />
* Extraction of p cola plasmid DNA - [https://2014.igem.org/Team:Paris_Saclay/Notebook/July/22#Extraction_of_p_cola_plasmid_DNA 22nd July]<br />
* Extraction and electrophoresis of BBa_K762100 with pSB1C3 - [https://2014.igem.org/Team:Paris_Saclay/Notebook/July/24#Plasmid_DNA_Extraction_&_Electrophoresis 24th July]<br />
* Gel electrophoresis of p cola - [https://2014.igem.org/Team:Paris_Saclay/Notebook/July/24#Gel_electrophoresis_of_p_cola 24th July]<br />
====pPS1====<br />
* PCR targeting with DY330 and pJBEI-6409 - [https://2014.igem.org/Team:Paris_Saclay/Notebook/July/22#PCR_Targeting 22nd July]<br />
* Transformation of DY330 with pJBEI-6409 - [https://2014.igem.org/Team:Paris_Saclay/Notebook/July/25#Transformation_of_electrocompetent_cells 25th July]<br />
* Liquid culture of DY330 - [https://2014.igem.org/Team:Paris_Saclay/Notebook/July/28#Liquid_Culture 28th July]<br />
* Transformation of DY330 with pJBEI-6409 - [https://2014.igem.org/Team:Paris_Saclay/Notebook/July/29#Transformation_of_electrocompetent_cells 29th July]<br />
* Culture of DY330 + pJBEI-6409 - [https://2014.igem.org/Team:Paris_Saclay/Notebook/July/31#D_-_Lemon_scent 31st July]<br />
* Transformation of DY330 with pJBEI-6409 - [https://2014.igem.org/Team:Paris_Saclay/Notebook/August/1#New_Transformation_of_electrocompetent_cells 1st July]<br />
* Electroporation of DY330+pJBEI-6409 - [https://2014.igem.org/Team:Paris_Saclay/Notebook/August/4#Electroporation 4th July]<br />
====pPS2-pPS3-pPS4====<br />
*PCR of the differents genes or Biobrick [https://2014.igem.org/Team:Paris_Saclay/Notebook/September/5#PCR_LS_PS_GS_Cad 5th Septembre] and [https://2014.igem.org/Team:Paris_Saclay/Notebook/September/8#Checking_PCR 8th September]<br />
* Cloning in Topo vector and transformation of competent E.coli [https://2014.igem.org/Team:Paris_Saclay/Notebook/September/8#Cloning 8th September] and [https://2014.igem.org/Team:Paris_Saclay/Notebook/September/11#ligation_of_PS_in_TOPO_vector_and_transformation 11th September]<br />
* Checking of the cloning [https://2014.igem.org/Team:Paris_Saclay/Notebook/September/9 9th September] and [https://2014.igem.org/Team:Paris_Saclay/Notebook/September/12 12th September]<br />
* Plasmids extraction [https://2014.igem.org/Team:Paris_Saclay/Notebook/September/10#Plasmid_extraction 10th Septembre]<br />
* Digestion to have the insert [https://2014.igem.org/Team:Paris_Saclay/Notebook/September/11#LS_and_GS_digestion 11th September] and [https://2014.igem.org/Team:Paris_Saclay/Notebook/September/16#Digestion_of_PS_topo_plasmids 16th September]<br />
* Ligation of the insert in the differents plasmids [https://2014.igem.org/Team:Paris_Saclay/Notebook/September/11#LS_and_GS_ligation 11th September] and [https://2014.igem.org/Team:Paris_Saclay/Notebook/September/16#Ligation 16th September]<br />
* Transformation [https://2014.igem.org/Team:Paris_Saclay/Notebook/September/15#Transformation_with_the_ligation_.28futur_pPS2.2F4.29 15th September] and [https://2014.igem.org/Team:Paris_Saclay/Notebook/September/17#transformation 17th September]<br />
* PCR on colony [https://2014.igem.org/Team:Paris_Saclay/Notebook/September/16#PCR_Colony 16th September] and [https://2014.igem.org/Team:Paris_Saclay/Notebook/September/18 18th September]<br />
* Checking of the insert sens [https://2014.igem.org/Team:Paris_Saclay/Notebook/September/19#PCR_to_check_the_sens_of_the_insert 19th September] and [https://2014.igem.org/Team:Paris_Saclay/Notebook/September/23 here] and [https://2014.igem.org/Team:Paris_Saclay/Notebook/September/24 24th September]<br />
* Final stock and extraction of pPS3 and pPS4 [https://2014.igem.org/Team:Paris_Saclay/Notebook/September/25 25 September]<br />
<br />
====pPS5====<br />
*Preparation of the pPS4 plasmid with SalI digestion [https://2014.igem.org/Team:Paris_Saclay/Notebook/September/24#SalI_digestion 24th September]<br />
*Ligation [https://2014.igem.org/Team:Paris_Saclay/Notebook/September/24#SalI_digestion 24 September]<br />
<br />
====PSBIC3====<br />
*Preparation of the plasmids [https://2014.igem.org/Team:Paris_Saclay/Notebook/September/11#Preparation_of_pSCBIC3_plasmid 11 September]<br />
<br />
===Salicylate Inducible Suppressing System===<br />
*Rehydration of BioBricks BBa_J61051 and BBa_K228001- [https://2014.igem.org/Team:Paris_Saclay/Notebook/July/23#The_Suppressing_Salicylate_System 23rd July]<br />
*Transformation of competent E.coli cells - [https://2014.igem.org/Team:Paris_Saclay/Notebook/July/24#Salicylate_Inducible_Suppressing_System 24th July]<br />
*Bacterial culture - [https://2014.igem.org/Team:Paris_Saclay/Notebook/July/25#Salicylate_Inducible_Suppressing_System 25th July]<br />
*Plasmid DNA Purification - [https://2014.igem.org/Team:Paris_Saclay/Notebook/July/28#Salicylate_Inducible_Suppressing_System 28th July]<br />
*Digestion to check & Electrophoresis - [https://2014.igem.org/Team:Paris_Saclay/Notebook/July/28#Salicylate_Inducible_Suppressing_System 28th July]<br />
*Main Digestion of BBa_J61051 and BBa_K228001 - [https://2014.igem.org/Team:Paris_Saclay/Notebook/July/29#Salicylate_Inducible_Suppressing_System 29th July]<br />
*Segregate Process - [https://2014.igem.org/Team:Paris_Saclay/Notebook/July/29#Salicylate_Inducible_Suppressing_System 29th July]<br />
*DNA purification gel agarose - [https://2014.igem.org/Team:Paris_Saclay/Notebook/July/30#Salicylate_Inducible_Suppressing_System 30th July]<br />
*Ligation - [https://2014.igem.org/Team:Paris_Saclay/Notebook/July/30#Salicylate_Inducible_Suppressing_System 30th July]<br />
*Transformation of competent E.coli cells - [https://2014.igem.org/Team:Paris_Saclay/Notebook/July/31#Salicylate_Inducible_Suppressing_System 31st July]<br />
*Liquid Culture - [https://2014.igem.org/Team:Paris_Saclay/Notebook/August/1#Salicylate_Inducible_Suppressing_System 1st August]<br />
*Plasmid DNA Purification - [https://2014.igem.org/Team:Paris_Saclay/Notebook/August/4#Salicylate_Inducible_Suppressing_System 4th August]<br />
*Digestion to check & Electrophoresis - [https://2014.igem.org/Team:Paris_Saclay/Notebook/August/4#Salicylate_Inducible_Suppressing_System 4th August]<br />
*Final Stock '''BBa_K1372000''' - [https://2014.igem.org/Team:Paris_Saclay/Notebook/August/4#Salicylate_Inducible_Suppressing_System 4th August]<br />
*Liquid Culture - [https://2014.igem.org/Team:Paris_Saclay/Notebook/August/5#Salicylate_Inducible_Suppressing_System 5th August]<br />
*Plasmid DNA Purification - [https://2014.igem.org/Team:Paris_Saclay/Notebook/August/6#Salicylate_Inducible_Suppressing_System 6th August]<br />
*Digestion of BBa_K1372000 and BBa_B0015- [https://2014.igem.org/Team:Paris_Saclay/Notebook/August/6#Salicylate_Inducible_Suppressing_System 6th August]<br />
*Segregate Process - [https://2014.igem.org/Team:Paris_Saclay/Notebook/August/6#Salicylate_Inducible_Suppressing_System 6th August]<br />
*DNA purification gel agarose - [https://2014.igem.org/Team:Paris_Saclay/Notebook/August/7#Salicylate_Inducible_Suppressing_System 7th August]<br />
*Ligation - [https://2014.igem.org/Team:Paris_Saclay/Notebook/August/7#Salicylate_Inducible_Suppressing_System 7th August]<br />
*Transformation of competent E.coli cells - [https://2014.igem.org/Team:Paris_Saclay/Notebook/August/8#Salicylate_Inducible_Suppressing_System 8th August]<br />
*Liquid Culture - [https://2014.igem.org/Team:Paris_Saclay/Notebook/August/11#Salicylate_Inducible_Suppressing_System 11th August]<br />
*Plasmid DNA Purification - [https://2014.igem.org/Team:Paris_Saclay/Notebook/August/12#Salicylate_Inducible_Suppressing_System 12th August]<br />
*Digestion to check & Electrophoresis - [https://2014.igem.org/Team:Paris_Saclay/Notebook/August/12#Salicylate_Inducible_Suppressing_System 12th August]<br />
*Final Stock '''BBa_K1372001''' - [https://2014.igem.org/Team:Paris_Saclay/Notebook/August/12#Salicylate_Inducible_Suppressing_System 12th August]<br />
<br />
===Construction of the Fusion Color Chromoprotein===<br />
* Plasmid DNA extraction and electrophoresis - [https://2014.igem.org/Team:Paris_Saclay/Notebook/July/24 24th July]<br />
* PCR of the Chromoprotein - [https://2014.igem.org/Team:Paris_Saclay/Notebook/August/18 18th August]<br />
* PCR Cloning in pGEMT easy - [https://2014.igem.org/Team:Paris_Saclay/Notebook/August/20 20th August]<br />
* Transformation of DH5α with chromoprotein (in pGEMT easy) - [https://2014.igem.org/Team:Paris_Saclay/Notebook/August/21 21st August]<br />
* Plasmid extraction - [https://2014.igem.org/Team:Paris_Saclay/Notebook/August/25 25th August]<br />
* Plasmid extraction - [https://2014.igem.org/Team:Paris_Saclay/Notebook/August/26 26th August]<br />
* Electrophoresis of pGEMT - [https://2014.igem.org/Team:Paris_Saclay/Notebook/August/27 27th August]<br />
* Transformation of DH5α by pSB1C3 - [https://2014.igem.org/Team:Paris_Saclay/Notebook/August/28 28th August]<br />
* Colony replication - [https://2014.igem.org/Team:Paris_Saclay/Notebook/September/1 1st September]<br />
* Liquid culture - [https://2014.igem.org/Team:Paris_Saclay/Notebook/September/2 2nd September]<br />
* Plasmid extraction and gel electrophoresis - [https://2014.igem.org/Team:Paris_Saclay/Notebook/September/3 3rd September]<br />
* PCR - [https://2014.igem.org/Team:Paris_Saclay/Notebook/September/5 5th September]<br />
* Check sequence of the w6 clone - [https://2014.igem.org/Team:Paris_Saclay/Notebook/September/8 8th September]<br />
* PCR Transformation for stock of the w6 clone - [https://2014.igem.org/Team:Paris_Saclay/Notebook/September/15 15th September]<br />
* PCR colony w6 clone, Liquid culture - [https://2014.igem.org/Team:Paris_Saclay/Notebook/September/16 16th September]<br />
<br />
===The Lemon Shaping===<br />
*Concentration Agar Test - [https://2014.igem.org/Team:Paris_Saclay/Notebook/June/30 30th June]<br />
*Concentration Agar Test - [https://2014.igem.org/Team:Paris_Saclay/Notebook/August/5 5th August]<br />
*Agar mold test - [https://2014.igem.org/Team:Paris_Saclay/Notebook/August/7 7th August]<br />
*Agar mold test - [https://2014.igem.org/Team:Paris_Saclay/Notebook/August/8 8th August]<br />
*Incubation of E. Coli with plasmid FNR RBS AmylCP in LB [https://2014.igem.org/Team:Paris_Saclay/Notebook/August/11 11th August]<br />
*Coloration of agar [https://2014.igem.org/Team:Paris_Saclay/Notebook/August/12 12th August]<br />
*Coloration of agar [https://2014.igem.org/Team:Paris_Saclay/Notebook/August/13 13th August]<br />
*Preparation of M63 medium [https://2014.igem.org/Team:Paris_Saclay/Notebook/August/14 14th August]<br />
* Incubation of E. Coli with plasmid FNR RBS AmylCP in M63 [https://2014.igem.org/Team:Paris_Saclay/Notebook/August/18 18th August]<br />
*Transformation of odor free E. coli with plasmids coding Fluo Protein [https://2014.igem.org/Team:Paris_Saclay/Notebook/August/27 27th August]<br />
* Results of Fluorescent Protein [https://2014.igem.org/Team:Paris_Saclay/Notebook/August/29 29th August]<br />
<br />
{{Team:Paris_Saclay/default_footer}}</div>Maschihttp://2014.igem.org/Team:Paris_Saclay/LabworkTeam:Paris Saclay/Labwork2014-10-18T02:03:04Z<p>Maschi: /* The E. coli odor-free chassis */</p>
<hr />
<div>{{Team:Paris_Saclay/labwork_header}}<br />
=Labwork=<br />
==Planning==<br />
We started the labwork in June. The first step was the research project scope and bibliography to ensure the feasibility of our project and to plan all the work we had to do during this wonderful iGEM summer. <br />
In early July we began the experiments, which was completed in the end of September. In order to be effective, we organized ourselves into four groups of summer interns. The groups were formed by all the members of the team, so even students from areas other than biology could cooperate for the project in the laboratory. To ensure the smooth development of the project by all students, the work had a life cycle : <br />
<br />
* Learning: A non biologist had to first learn the basics of biology to understand the lab work.<br />
* Practice: The novice starts to do their experiments with the help of another biology student or an adviser.<br />
* Development: The aforementioned student becomes autonomous and takes initiative.<br />
* Transmission of acquired knowledge: The student, initially oblivious to practices in the lab, can transmit this knowledge at the end of their internship to newcomers.<br />
<br />
===The ''E. coli'' odor-free chassis===<br />
*Culture of MG1655 and MG1655Z1 strains- [https://2014.igem.org/Team:Paris_Saclay/Notebook/June/30 30st July]<br />
*P1 phage stock preparation for the transduction of the ''Delta-tnaA::Kan'' - [https://2014.igem.org/Team:Paris_Saclay/Notebook/July/2 2nd July]<br />
*P1 phage transduction using the stock prepared on July 2nd to MG1655 and MG1655Z1 strains - [https://2014.igem.org/Team:Paris_Saclay/Notebook/July/3 3rd July]<br />
*Cultures of MG1655 and MG1655Z1 transductants - [https://2014.igem.org/Team:Paris_Saclay/Notebook/July/4 4st July]<br />
*Transformations test of competent cells MG1655 and MG1655Z1 - [https://2014.igem.org/Team:Paris_Saclay/Notebook/July/10 10th July]<br />
*Preparation of competent cells E coli, deletion of the antibiotic cassette in the odorless bacteria - [https://2014.igem.org/Team:Paris_Saclay/Notebook/July/11 11th July]<br />
*Preparation of competent cells E. coli MG1655 and MG1655Z1 transductants - [https://2014.igem.org/Team:Paris_Saclay/Notebook/July/17 17th July]<br />
*Transformation of supercompetent cells MG1655 and MG1655Z1 transductants with plasmid BT340- [https://2014.igem.org/Team:Paris_Saclay/Notebook/July/18 18th July]<br />
*Results of the transformation - [https://2014.igem.org/Team:Paris_Saclay/Notebook/July/21 21st July]<br />
*Preparation and transformation of competent MG1655 and MG1655Z1 transductants - [https://2014.igem.org/Team:Paris_Saclay/Notebook/July/22 22nd July]<br />
*PCR verification of the strains grown - [https://2014.igem.org/Team:Paris_Saclay/Notebook/July/25 25th July]<br />
*Preparation and Transformation of competent MG1655 and MG1655Z1 with BT340 - [https://2014.igem.org/Team:Paris_Saclay/Notebook/July/28 28th July]<br />
*PCR of MG1655 and MG1655Z1 with FTR-Apra-F and FTR-Apra-R - [https://2014.igem.org/Team:Paris_Saclay/Notebook/July/29 29th July]<br />
*Preparation and transformation of electrocompetent MG1655 and MG1655Z1 with plasmid BT340 - [https://2014.igem.org/Team:Paris_Saclay/Notebook/July/30 30st July]<br />
*Clone isolation by streak of ''Delta-tnaA'' MG1655 and MG1655Z1 on LB dishes - [https://2014.igem.org/Team:Paris_Saclay/Notebook/August/1 1st August]<br />
*Clone isolation by streak of ''Delta-tnaA'' MG1655 and MG1655Z1 on LB and kan dishes - [https://2014.igem.org/Team:Paris_Saclay/Notebook/August/4 4th August]<br />
*PCR verification of the final strains ''Delta-tnaA'' MG1655 and MG1655Z1 - [https://2014.igem.org/Team:Paris_Saclay/Notebook/August/5 5th August]<br />
*Final stock of MG1655 ''Delta-tnaA'' - [https://2014.igem.org/Team:Paris_Saclay/Notebook/August/6 6st August]<br />
<br />
===The Lemon Scent===<br />
====Preparation====<br />
* Rehydration of BioBricks BBa_J45014, BBa_K517003 - [https://2014.igem.org/Team:Paris_Saclay/Notebook/June/30 here]<br />
* Preparation of electrocompetent DY330 and transformation via pJBEI-6409 - [https://2014.igem.org/Team:Paris_Saclay/Notebook/July/18#Preparation_of_electrocompetent_DY330_and_transformation_via_pJBEI6409 here]<br />
* Extraction of p cola plasmid DNA - [https://2014.igem.org/Team:Paris_Saclay/Notebook/July/22#Extraction_of_p_cola_plasmid_DNA here]<br />
* Extraction and electrophoresis of BBa_K762100 with pSB1C3 - [https://2014.igem.org/Team:Paris_Saclay/Notebook/July/24#Plasmid_DNA_Extraction_&_Electrophoresis here]<br />
* Gel electrophoresis of p cola - [https://2014.igem.org/Team:Paris_Saclay/Notebook/July/24#Gel_electrophoresis_of_p_cola here]<br />
====pPS1====<br />
* PCR targeting with DY330 and pJBEI-6409 - [https://2014.igem.org/Team:Paris_Saclay/Notebook/July/22#PCR_Targeting here]<br />
* Transformation of DY330 with pJBEI-6409 - [https://2014.igem.org/Team:Paris_Saclay/Notebook/July/25#Transformation_of_electrocompetent_cells here]<br />
* Liquid culture of DY330 - [https://2014.igem.org/Team:Paris_Saclay/Notebook/July/28#Liquid_Culture here]<br />
* Transformation of DY330 with pJBEI-6409 - [https://2014.igem.org/Team:Paris_Saclay/Notebook/July/29#Transformation_of_electrocompetent_cells 29th here]<br />
* Culture of DY330 + pJBEI-6409 - [https://2014.igem.org/Team:Paris_Saclay/Notebook/July/31#D_-_Lemon_scent here]<br />
* Transformation of DY330 with pJBEI-6409 - [https://2014.igem.org/Team:Paris_Saclay/Notebook/August/1#New_Transformation_of_electrocompetent_cells here]<br />
* Electroporation of DY330+pJBEI-6409 - [https://2014.igem.org/Team:Paris_Saclay/Notebook/August/4#Electroporation here]<br />
====pPS2-pPS3-pPS4====<br />
*PCR of the differents genes or Biobrick [https://2014.igem.org/Team:Paris_Saclay/Notebook/September/5#PCR_LS_PS_GS_Cad Here]and [https://2014.igem.org/Team:Paris_Saclay/Notebook/September/8#Checking_PCR here]<br />
* Cloning in Topo vector and transformation of competent E.coli [https://2014.igem.org/Team:Paris_Saclay/Notebook/September/8#Cloning here] and [https://2014.igem.org/Team:Paris_Saclay/Notebook/September/11#ligation_of_PS_in_TOPO_vector_and_transformation here]<br />
* Checking of the cloning [https://2014.igem.org/Team:Paris_Saclay/Notebook/September/9 here] and [https://2014.igem.org/Team:Paris_Saclay/Notebook/September/12 here]<br />
* Plasmids extraction [https://2014.igem.org/Team:Paris_Saclay/Notebook/September/10#Plasmid_extraction here]<br />
* Digestion to have the insert [https://2014.igem.org/Team:Paris_Saclay/Notebook/September/11#LS_and_GS_digestion here] and [https://2014.igem.org/Team:Paris_Saclay/Notebook/September/16#Digestion_of_PS_topo_plasmids here]<br />
* Ligation of the insert in the differents plasmids [https://2014.igem.org/Team:Paris_Saclay/Notebook/September/11#LS_and_GS_ligation here] and [https://2014.igem.org/Team:Paris_Saclay/Notebook/September/16#Ligation Here]<br />
* Transformation [https://2014.igem.org/Team:Paris_Saclay/Notebook/September/15#Transformation_with_the_ligation_.28futur_pPS2.2F4.29 Here] and [https://2014.igem.org/Team:Paris_Saclay/Notebook/September/17#transformation here]<br />
* PCR on colony [https://2014.igem.org/Team:Paris_Saclay/Notebook/September/16#PCR_Colony here] and [https://2014.igem.org/Team:Paris_Saclay/Notebook/September/18 here]<br />
* Checking of the insert sens [https://2014.igem.org/Team:Paris_Saclay/Notebook/September/19#PCR_to_check_the_sens_of_the_insert here] and [https://2014.igem.org/Team:Paris_Saclay/Notebook/September/23 here] and [https://2014.igem.org/Team:Paris_Saclay/Notebook/September/24 here]<br />
* Final stock and extraction of pPS3 and pPS4 [https://2014.igem.org/Team:Paris_Saclay/Notebook/September/25 here]<br />
<br />
====pPS5====<br />
*Preparation of the pPS4 plasmid with SalI digestion [https://2014.igem.org/Team:Paris_Saclay/Notebook/September/24#SalI_digestion here]<br />
*Ligation [https://2014.igem.org/Team:Paris_Saclay/Notebook/September/24#SalI_digestion here]<br />
<br />
====PSBIC3====<br />
*Preparation of the plasmids [https://2014.igem.org/Team:Paris_Saclay/Notebook/September/11#Preparation_of_pSCBIC3_plasmid here]<br />
<br />
===Salicylate Inducible Suppressing System===<br />
*Rehydration of BioBricks BBa_J61051 and BBa_K228001- [https://2014.igem.org/Team:Paris_Saclay/Notebook/July/23#The_Suppressing_Salicylate_System 23rd July]<br />
*Transformation of competent E.coli cells - [https://2014.igem.org/Team:Paris_Saclay/Notebook/July/24#Salicylate_Inducible_Suppressing_System 24th July]<br />
*Bacterial culture - [https://2014.igem.org/Team:Paris_Saclay/Notebook/July/25#Salicylate_Inducible_Suppressing_System 25th July]<br />
*Plasmid DNA Purification - [https://2014.igem.org/Team:Paris_Saclay/Notebook/July/28#Salicylate_Inducible_Suppressing_System 28th July]<br />
*Digestion to check & Electrophoresis - [https://2014.igem.org/Team:Paris_Saclay/Notebook/July/28#Salicylate_Inducible_Suppressing_System 28th July]<br />
*Main Digestion of BBa_J61051 and BBa_K228001 - [https://2014.igem.org/Team:Paris_Saclay/Notebook/July/29#Salicylate_Inducible_Suppressing_System 29th July]<br />
*Segregate Process - [https://2014.igem.org/Team:Paris_Saclay/Notebook/July/29#Salicylate_Inducible_Suppressing_System 29th July]<br />
*DNA purification gel agarose - [https://2014.igem.org/Team:Paris_Saclay/Notebook/July/30#Salicylate_Inducible_Suppressing_System 30th July]<br />
*Ligation - [https://2014.igem.org/Team:Paris_Saclay/Notebook/July/30#Salicylate_Inducible_Suppressing_System 30th July]<br />
*Transformation of competent E.coli cells - [https://2014.igem.org/Team:Paris_Saclay/Notebook/July/31#Salicylate_Inducible_Suppressing_System 31st July]<br />
*Liquid Culture - [https://2014.igem.org/Team:Paris_Saclay/Notebook/August/1#Salicylate_Inducible_Suppressing_System 1st August]<br />
*Plasmid DNA Purification - [https://2014.igem.org/Team:Paris_Saclay/Notebook/August/4#Salicylate_Inducible_Suppressing_System 4th August]<br />
*Digestion to check & Electrophoresis - [https://2014.igem.org/Team:Paris_Saclay/Notebook/August/4#Salicylate_Inducible_Suppressing_System 4th August]<br />
*Final Stock '''BBa_K1372000''' - [https://2014.igem.org/Team:Paris_Saclay/Notebook/August/4#Salicylate_Inducible_Suppressing_System 4th August]<br />
*Liquid Culture - [https://2014.igem.org/Team:Paris_Saclay/Notebook/August/5#Salicylate_Inducible_Suppressing_System 5th August]<br />
*Plasmid DNA Purification - [https://2014.igem.org/Team:Paris_Saclay/Notebook/August/6#Salicylate_Inducible_Suppressing_System 6th August]<br />
*Digestion of BBa_K1372000 and BBa_B0015- [https://2014.igem.org/Team:Paris_Saclay/Notebook/August/6#Salicylate_Inducible_Suppressing_System 6th August]<br />
*Segregate Process - [https://2014.igem.org/Team:Paris_Saclay/Notebook/August/6#Salicylate_Inducible_Suppressing_System 6th August]<br />
*DNA purification gel agarose - [https://2014.igem.org/Team:Paris_Saclay/Notebook/August/7#Salicylate_Inducible_Suppressing_System 7th August]<br />
*Ligation - [https://2014.igem.org/Team:Paris_Saclay/Notebook/August/7#Salicylate_Inducible_Suppressing_System 7th August]<br />
*Transformation of competent E.coli cells - [https://2014.igem.org/Team:Paris_Saclay/Notebook/August/8#Salicylate_Inducible_Suppressing_System 8th August]<br />
*Liquid Culture - [https://2014.igem.org/Team:Paris_Saclay/Notebook/August/11#Salicylate_Inducible_Suppressing_System 11th August]<br />
*Plasmid DNA Purification - [https://2014.igem.org/Team:Paris_Saclay/Notebook/August/12#Salicylate_Inducible_Suppressing_System 12th August]<br />
*Digestion to check & Electrophoresis - [https://2014.igem.org/Team:Paris_Saclay/Notebook/August/12#Salicylate_Inducible_Suppressing_System 12th August]<br />
*Final Stock '''BBa_K1372001''' - [https://2014.igem.org/Team:Paris_Saclay/Notebook/August/12#Salicylate_Inducible_Suppressing_System 12th August]<br />
<br />
===Construction of the Fusion Color Chromoprotein===<br />
* Plasmid DNA extraction and electrophoresis - [https://2014.igem.org/Team:Paris_Saclay/Notebook/July/24 24th July]<br />
* PCR of the Chromoprotein - [https://2014.igem.org/Team:Paris_Saclay/Notebook/August/18 18th August]<br />
* PCR Cloning in pGEMT easy - [https://2014.igem.org/Team:Paris_Saclay/Notebook/August/20 20th August]<br />
* Transformation of DH5α with chromoprotein (in pGEMT easy) - [https://2014.igem.org/Team:Paris_Saclay/Notebook/August/21 21st August]<br />
* Plasmid extraction - [https://2014.igem.org/Team:Paris_Saclay/Notebook/August/25 25th August]<br />
* Plasmid extraction - [https://2014.igem.org/Team:Paris_Saclay/Notebook/August/26 26th August]<br />
* Electrophoresis of pGEMT - [https://2014.igem.org/Team:Paris_Saclay/Notebook/August/27 27th August]<br />
* Transformation of DH5α by pSB1C3 - [https://2014.igem.org/Team:Paris_Saclay/Notebook/August/28 28th August]<br />
* Colony replication - [https://2014.igem.org/Team:Paris_Saclay/Notebook/September/1 1st September]<br />
* Liquid culture - [https://2014.igem.org/Team:Paris_Saclay/Notebook/September/2 2nd September]<br />
* Plasmid extraction and gel electrophoresis - [https://2014.igem.org/Team:Paris_Saclay/Notebook/September/3 3rd September]<br />
* PCR - [https://2014.igem.org/Team:Paris_Saclay/Notebook/September/5 5th September]<br />
* Check sequence of the w6 clone - [https://2014.igem.org/Team:Paris_Saclay/Notebook/September/8 8th September]<br />
* PCR Transformation for stock of the w6 clone - [https://2014.igem.org/Team:Paris_Saclay/Notebook/September/15 15th September]<br />
* PCR colony w6 clone, Liquid culture - [https://2014.igem.org/Team:Paris_Saclay/Notebook/September/16 16th September]<br />
<br />
===The Lemon Shaping===<br />
*Concentration Agar Test - [https://2014.igem.org/Team:Paris_Saclay/Notebook/June/30 here]<br />
*Concentration Agar Test - [https://2014.igem.org/Team:Paris_Saclay/Notebook/August/5 here]<br />
*Agar mold test - [https://2014.igem.org/Team:Paris_Saclay/Notebook/August/7 here]<br />
*Agar mold test - [https://2014.igem.org/Team:Paris_Saclay/Notebook/August/8 here]<br />
*Incubation of E. Coli with plasmid FNR RBS AmylCP in LB [https://2014.igem.org/Team:Paris_Saclay/Notebook/August/11 here]<br />
*Coloration of agar [https://2014.igem.org/Team:Paris_Saclay/Notebook/August/12 here]<br />
*Coloration of agar [https://2014.igem.org/Team:Paris_Saclay/Notebook/August/13 here]<br />
*Preparation of M63 medium [https://2014.igem.org/Team:Paris_Saclay/Notebook/August/14 here]<br />
* Incubation of E. Coli with plasmid FNR RBS AmylCP in M63 [https://2014.igem.org/Team:Paris_Saclay/Notebook/August/18#Monday_18th_August here]<br />
*Transformation of odor free E. coli with plasmids coding Fluo Protein [https://2014.igem.org/Team:Paris_Saclay/Notebook/August/27 here]<br />
* Results of Fluorescent Protein [https://2014.igem.org/Team:Paris_Saclay/Notebook/August/29#Friday_29th_August here]<br />
<br />
{{Team:Paris_Saclay/default_footer}}</div>Maschihttp://2014.igem.org/Team:Paris_Saclay/ProjectTeam:Paris Saclay/Project2014-10-18T01:55:32Z<p>Maschi: /* Project */</p>
<hr />
<div>{{Team:Paris_Saclay/project_header}}<br />
=Project=<br />
<br />
IGEM teams use live organism systems to perform specific functions, as mechanics build a machine. Working with life still raises a lot of ethical questions like the representation of life and our team has chosen Bio-Art to explore this subject. To illustrate these questions, we chose an object "made-in-nature", a lemon, and we tried to make an equivalent "made-in-IGEM". This object has the same external properties of a lemon: smell, form and a colour that slowly changes from green to yellow, like a lemon when it ripens.<br />
Moreover we use [https://2014.igem.org/Team:Paris_Saclay/Project/Fastlemon Fastlemon] to raise ethicals issues.<br />
<br />
==Video==<br />
In a collaboration with iGEM Paris-Bettencourt team in our [https://2014.igem.org/Team:Paris_Saclay/Project/Workshop iGEM French Meeting], we made a video to introduce the project.<br />
<html><div style="width:560px; margin:0 auto;"><iframe width="560" height="315" src="//www.youtube.com/embed/oYY6H_yvft0" frameborder="0" allowfullscreen></iframe></div></html><br />
<br />
==[https://2014.igem.org/Team:Paris_Saclay/Project/Inspirations Inspirations]==<br />
<br />
== Scientific part==<br />
Our project is based on the construction of a bacterial lemon that will look like lemon but made only of ''Escherichia coli'' modified to produce a lemon scent and to change colour from green to yellow. We will achieve our project by genetically modifying ''Escherichia coli'' to be devoid of any unpleasant odour by deleting the genes involved in ''E. coli'''s odor, by introducing genes for the production of the lemon scent and finally by adding a yellow-blue chromoprotein whose expression is controlled by a tRNA suppressor for the colour switching.<br />
<br />
===[https://2014.igem.org/Team:Paris_Saclay/Project/Odor-free_ecoli Remove the bad smell of ''E. coli'']===<br />
Our lemon should smell like a lemon. Our chassis is ''Escherichia coli'' , a bacterium known to have a foul odor. We thus have to remove the genes involved in this particular phenotype. The ''tnaA'' gene is required for the degradation of tryptophan into indole, the main molecule at the origin of ''E. coli'' smell. We use a phage transduction method to reach our goal.<br />
<br />
===[https://2014.igem.org/Team:Paris_Saclay/Project/Lemon_Scent Lemon Scent]===<br />
The particular odor of lemon is mainly due to 3 monoterpenes: limonene, beta-pinene and geranial. We will clone the 3 monoterpene synthases responsible for the production of these 3 molecules into our bacteria to allow them to produce this fragrance. We will also improve the production of the GPP precursor using a synthetic mevalonate pathway using the construction of the team of T. S. Lee.<br />
<br />
===[https://2014.igem.org/Team:Paris_Saclay/Project/Salicylate_Inducible_System Lemon appearance and Ripening]===<br />
Since vision is one of the most important senses in humans, we want the lemon to look as real as possible. To achieve this, we plan to make our lemon look green or yellow, like a real one. Furthermore, we also want to simulate the ripening process of the lemon by changing its color gradually from green to yellow.<br />
<br />
==Artistic Part==<br />
===[https://2014.igem.org/Team:Paris_Saclay/Project/Lemon_Shaping Lemon Shaping]===<br />
<br />
1st idea<br />
<br />
We basically wanted to build up a lemon tree in which one we would have put lemons we had made. As lemons pass from green to yellow, we would have simulated the season’s cycle.<br />
<br />
===Boston Installation===<br />
<br />
2nd idea<br />
<br />
The idea was to project the shadow of a little sculpture in a screen or a wall: the positioning of the light with respect to the sculpture would have enabled the creation of shadow ten times bigger than the sculpture itself. The aim of this structure would have been to show the gap between a object and its image, between real things and decoys.<br />
<br />
<br />
3rd idea (chosen for Boston)<br />
<br />
Finally we decided to modify the second idea (for logistic reasons). The element which could this time reveal the gap between an object and its image, between real things and decoys, would be a mirror … <br />
<br />
{{Team:Paris_Saclay/default_footer}}</div>Maschihttp://2014.igem.org/Team:Paris_Saclay/ProjectTeam:Paris Saclay/Project2014-10-18T01:54:54Z<p>Maschi: /* Countdown */</p>
<hr />
<div>{{Team:Paris_Saclay/project_header}}<br />
=Project=<br />
<br />
IGEM teams use live organism systems to perform specific functions, as mechanics build a machine. Working with life still raises a lot of ethical questions like the representation of life and our team has chosen Bio-Art to explore this subject. To illustrate these questions, we chose an object "made-in-nature", a lemon, and we tried to make an equivalent "made-in-IGEM". This object has the same external properties of a lemon: smell, form and a colour that slowly changes from green to yellow, like a lemon when it ripens.<br />
Moreover we use [https://2014.igem.org/Team:Paris_Saclay/Project/Fastlemon Fastlemon] to raise ethicals issues.<br />
<br />
==Video==<br />
In a collaboration with iGEM Paris-Bettencourt team in our [https://2014.igem.org/Team:Paris_Saclay/Project/Workshop iGEM French Meeting], we made a video to introduce the project.<br />
<html><div style="width:560px; margin:0 auto;"><iframe width="560" height="315" src="//www.youtube.com/embed/oYY6H_yvft0" frameborder="0" allowfullscreen></iframe></div></html><br />
<br />
==[https://2014.igem.org/Team:Paris_Saclay/Project/Inspirations Inspirations]==<br />
<br />
== Scientific part==<br />
Our project is based on the construction of a bacterial lemon that will look like lemon but made only of ''Escherichia coli'' modified to produce a lemon scent and to change colour from green to yellow. We will achieve our project by genetically modifying ''Escherichia coli'' to be devoid of any unpleasant odour by deleting the genes involved in ''E. coli'''s odor, by introducing genes for the production of the lemon scent and finally by adding a yellow-blue chromoprotein whose expression is controlled by a tRNA suppressor for the colour switching.<br />
<br />
===[https://2014.igem.org/Team:Paris_Saclay/Project/Odor-free_ecoli Remove the bad smell of ''E. coli'']===<br />
Our lemon should smell like a lemon. Our chassis is ''Escherichia coli'' , a bacterium known to have a foul odor. We thus have to remove the genes involved in this particular phenotype. The ''tnaA'' gene is required for the degradation of tryptophan into indole, the main molecule at the origin of ''E. coli'' smell. We use a phage transduction method to reach our goal.<br />
<br />
===[https://2014.igem.org/Team:Paris_Saclay/Project/Lemon_Scent Lemon Scent]===<br />
The particular odor of lemon is mainly due to 3 monoterpenes: limonene, beta-pinene and geranial. We will clone the 3 monoterpene synthases responsible for the production of these 3 molecules into our bacteria to allow them to produce this fragrance. We will also improve the production of the GPP precursor using a synthetic mevalonate pathway using the construction of the team of T. S. Lee.<br />
<br />
===[https://2014.igem.org/Team:Paris_Saclay/Project/Salicylate_Inducible_System Lemon appearance and Ripening]===<br />
Since vision is one of the most important senses in humans, we want the lemon to look as real as possible. To achieve this, we plan to make our lemon look green or yellow, like a real one. Furthermore, we also want to simulate the ripening process of the lemon by changing its color gradually from green to yellow.<br />
<br />
==Artistic Part==<br />
===[https://2014.igem.org/Team:Paris_Saclay/Project/Lemon_Shaping Lemon Shaping]===<br />
<br />
1st idea<br />
<br />
We basically wanted to build up a lemon tree in which one we would have put lemons we had made. As lemons pass from green to yellow, we would have simulated the season’s cycle.<br />
<br />
===Boston Installation===<br />
<br />
2nd idea<br />
<br />
The idea was to project the shadow of a little sculpture in a screen or a wall: the positioning of the light with respect to the sculpture would have enabled the creation of shadow ten times bigger than the sculpture itself. The aim of this structure would have been to show the gap between a object and its image, between real things and decoys.<br />
<br />
<br />
<br />
3rd idea (chosen for Boston)<br />
<br />
Finally we decided to modify the second idea (for logistic reasons). The element which could this time reveal the gap between an object and its image, between real things and decoys, would be a mirror … <br />
<br />
<br />
====Countdown====<br />
This page is under '''Leïla''''s responsibility<br />
<br />
* Deadline: 08/oct.<br />
** Text<br />
* Deadline: 12/oct<br />
** Final review by Solenne.<br />
<br />
<br />
{{Team:Paris_Saclay/default_footer}}</div>Maschihttp://2014.igem.org/Team:Paris_Saclay/SafetyTeam:Paris Saclay/Safety2014-10-18T01:53:19Z<p>Maschi: </p>
<hr />
<div>{{Team:Paris_Saclay/labwork_header}}<br />
=Safety=<br />
<br />
==In the Lab==<br />
Our project uses strains and reagents that are used in biosafety level 1 laboratories; we use non-pathogenic derivatives of E. coli strain K-12 (in the event of accidental or otherwise release, the health risks are minimal). <br />
Our BioBricks are not supposed to confer a pathogenic attributes to E. coli, and there is no data to suggest that they could have any detrimental effects on the environment.<br />
At that level, there are no special precautions required, other than those specified for work in laboratory: no food and drinks in the laboratory, gloves and other appropriate personal protective equipment worn during experiments. Our project involves regular use of BET, a DNA-intercaling agent known to cause cancer, and the use of UV light, for visualization of electrophoresis gel. We must prepare culture with antibiotics, which could be harmful to humans in large doses. We also work with Bunsen burner to maintain a sterile environment, which do involve having an open flame on the lab bench. The laboratory is equipped in case of fire. Students participating in this project received safety training to begin the project. The safety training consisted of a presentation covering the various aspects of safety found in molecular biology laboratories. Students were also supervised by instructors throughout the duration of the project.<br />
<br />
[[File:Paris Saclay Safety.jpg|500px|centre]]<br />
<br />
==Safety form:==<br />
===Your Training===<br />
''a) Have your team members received any safety training yet?''<br />
<br />
Yes, we have already received safety training.<br />
<br />
''b) Please briefly describe the topics that you learned about (or will learn about) in your safety training.''<br />
<br />
How to safely work with UV rays, BET, phenols, gases. How to correctly dispose of biological waste. <br />
<br />
''c) Please give a link to the laboratory safety training requirements of your institution (college, university, community lab, etc). Or, if you cannot give a link, briefly describe the requirements.'' <br />
<br />
* Read the rules of procedure of IGM (Institut de Genetique et de Microbiologie)<br />
* Read the CNRS guidelines at http://www.dgdr.cnrs.fr/SST/CNPS/guides/risques/guide.htm<br />
* Meet BioSafety Officer and visit the laboratory<br />
<br />
===Your Local Rules and Regulations===<br />
<br />
''a) Who is responsible for biological safety at your institution? (You might have an Institutional Biosafety Committee, an Office of Environmental Health and Safety, a single Biosafety Officer, or some other arrangement.) Have you discussed your project with them? Describe any concerns they raised, and any changes you made in your project based on your discussion.''<br />
<br />
Biosafety Officer ACMO (IGM Institut de Genetique et de Microbiologie Service Hygiene et Securite) Florence Lorieux. <br />
<br />
''b) What are the biosafety guidelines of your institution? Please give a link to these guidelines, or briefly describe them if you cannot give a link.''<br />
<br />
* http://www.sciences.u-psud.fr/fr/la_faculte/service_d_hygiene_et_de_securite.html<br />
* http://www.dgdr.cnrs.fr/SST/CNPS/guides/risques/guide.htm<br />
<br />
''c) In your country, what are the regulations that govern biosafety in research laboratories? Please give a link to these regulations, or briefly describe them if you cannot give a link.''<br />
<br />
* http://www.inrs.fr/accueil/risques/biologiques/prevention-risques/cadre-prevention.html<br />
* http://www.hautconseildesbiotechnologies.fr/IMG/pdf/Manuel_HCB_utilisation_confinee_OGM.pdf<br />
<br />
===The Organisms and Parts that You Use===<br />
<br />
Please visit this page to download a blank copy of the spreadsheet for question 3. (If you need a CSV version instead of XLS, visit this page.)<br />
Complete the spreadsheet. Include all whole organisms that you will handle in the lab, whether you are using them as a chassis or for some other reason. Include all new or highly modified protein coding parts that you are using. If you submitted a Check-In for an organism or part, you should still include it in this spreadsheet.<br />
You may omit non-protein-coding parts, and you may omit parts that were already in the Registry if you are using them without significant modifications.<br />
<br />
Click here to show/hide instructions for completing the spreadsheet<br />
https://2014.igem.org/File:Paris_Saclay_Safety2014_Spreadsheet.xls<br />
<br />
===Risks of Your Project Now===<br />
<br />
Please describe risks of working with the biological materials (cells, organisms, DNA, etc.) that you are using in your project. If you are taking any safety precautions (even basic ones, like rubber gloves), that is because your work has some risks, however small. Therefore, please discuss possible risks and what you have done (or might do) to minimize them, instead of simply saying that there are no risks at all.<br />
<br />
''a) Risks to the safety and health of team members, or other people working in the lab:''<br />
<br />
All bacteria (Escherichia coli K12 and Escherichia coli MG1655Z1) used in our project belong to the risk group 1, so they do not represent any risk for the health of the members of the team. The only real risks come from certain chemicals and the Bunsen burners.<br />
<br />
''b) Risks to the safety and health of the general public (if any biological materials escaped from your lab):''<br />
<br />
Our final system or any of the intermediate strains constructed do not represent any risk or danger for the general public as they are all derived from Escherichia coli K12 and Escherichia coli MG1655Z1 (belonging to the risk group 1), strains that cannot colonize the human colon and does not produce toxins.<br />
<br />
''c) Risks to the environment (from waste disposal, or from materials escaping from your lab):''<br />
<br />
The strains used and constructed derive from strains of E. coli with low levels of survival outside the laboratory. The bacteria do not produce harmful compounds either.<br />
<br />
''d) Risks to security through malicious mis-use by individuals, groups, or countries:''<br />
<br />
The genes used in this system do not code for toxins or for enzymes synthesizing products toxic to humans or harmful for the environment.<br />
<br />
''e) What measures are you taking to reduce these risks? (For example: safe lab practices, choices of which organisms to use.)''<br />
<br />
We wear gloves when using BET or other toxic chemicals, under a fume hood.<br />
<br />
===Risks of Your Project in the Future===<br />
<br />
What would happen if all your dreams came true, and your project grew from a small lab study into a commercial/industrial/medical product that was used by many people? We invite you to speculate broadly and discuss possibilities, rather than providing definite answers. Even if the product is "safe", please discuss possible risks and how they could be addressed, rather than simply saying that there are no risks at all.<br />
<br />
''a) What new risks might arise from your project's growth? (Consider the categories of risk listed in parts a-d of the previous question: lab workers, the general public, the environment, and malicious mis-uses.) Also, what risks might arise if the knowledge you generate or the methodsyou develop became widely available?''<br />
<br />
Our project is a BioArt project, therefore is not designed for mass production and/or commercialization. <br />
<br />
''b) Does your project currently include any design features to reduce risks? Or, if you did all the future work to make your project grow into a popular product, would you plan to design any new features to minimize risks? (For example: auxotrophic chassis, physical containment, etc.)''<br />
Such features are not required for an iGEM project, but many teams choose to explore them.<br />
Even though we are working on a BioArt project, we are aware that we use GMOs. We intend to keep our bacteria in a confined state to prevent our work from contamination from the outside and vice versa.<br />
{{Team:Paris_Saclay/default_footer}}</div>Maschihttp://2014.igem.org/File:Paris_Saclay_Safety.jpgFile:Paris Saclay Safety.jpg2014-10-18T01:52:56Z<p>Maschi: </p>
<hr />
<div></div>Maschihttp://2014.igem.org/Team:Paris_Saclay/ProtocolsTeam:Paris Saclay/Protocols2014-10-18T01:49:46Z<p>Maschi: /* Countdown */</p>
<hr />
<div>{{Team:Paris_Saclay/labwork_header}}<br />
=Protocols=<br />
<br />
#[https://2014.igem.org/Team:Paris_Saclay/Protocols/Preparation_of_supercompetent_Ecoli_cells Preparation of supercompetent ''E. coli'' cells]<br />
#[https://2014.igem.org/Team:Paris_Saclay/Protocols/Transformation_of_supercompetent_Ecoli_cells Transformation of CaCl2 supercompetent ''E. coli'' cells]<br />
#[https://2014.igem.org/Team:Paris_Saclay/Protocols/Extraction_of_the_Genomic_DNA_from_Bacteria_by_using_NucleoSpin®_Tissue Extraction of the Plasmid DNA from Bacteria by using NucleoSpin® Plasmid]<br />
#[https://2014.igem.org/Team:Paris_Saclay/Protocols/Extraction_of_the_Genomic_DNA_from_Bacteria_without_NucleoSpin®_Tissue Extraction of the Plasmid DNA from Bacteria without NucleoSpin® Plasmid]<br />
#[https://2014.igem.org/Team:Paris_Saclay/Protocols/Gel_Electrophoresis Gel Electrophoresis]<br />
#[https://2014.igem.org/Team:Paris_Saclay/Protocols/Transformation_of_supercompetent_Ecoli_cells_with_CaCl2 Competent ''E. coli'' cells]<br />
#[https://2014.igem.org/Team:Paris_Saclay/Protocols/PCR_for_the_genomic_DNA_of_bacteria PCR for the genomic DNA of bacteria]<br />
#[https://2014.igem.org/Team:Paris_Saclay/Protocols/PCR_clean-up PCR clean-up]<br />
#[https://2014.igem.org/Team:Paris_Saclay/Protocols/PCR_for_bacterial_culture PCR for bacterial culture]<br />
#[https://2014.igem.org/Team:Paris_Saclay/Protocols/Transformation_of_competent_cells_by_electroporation Transformation of competent cells by electroporation]<br />
#[https://2014.igem.org/Team:Paris_Saclay/Protocols/BioBrick_Assembly BioBrick Assembly]<br />
#[https://2014.igem.org/Team:Paris_Saclay/Protocols/P1_phages_stock P1 Phages Stock]<br />
#[https://2014.igem.org/Team:Paris_Saclay/Protocols/P1_phages_transduction P1 phages transduction]<br />
#[https://2014.igem.org/Team:Paris_Saclay/Protocols/Transformation_of_competent_e.coli_by_CaCl2 Preparation and Transformation of competent ''E. coli'' by CaCl2]<br />
#[https://2014.igem.org/Team:Paris_Saclay/Protocols/Cloning_in_pGEMTeasy Cloning in pGEMTeasy]<br />
#[https://2014.igem.org/Team:Paris_Saclay/Protocols/Electro_elution_of_DNA_from_agarose_gel Electro elution of DNA from agarose gel]<br />
<br />
{{Team:Paris_Saclay/protocols_footer}}</div>Maschihttp://2014.igem.org/Team:Paris_Saclay/NotebookTeam:Paris Saclay/Notebook2014-10-18T01:48:26Z<p>Maschi: /* Labwork */</p>
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=Labwork=<br />
==Notebook==<br />
We constituted our team in November 2013 and we had meetings twice a month until April. Then we met every week. The Lab work started in the beginning of June.<br />
<br />
Use our calendar to have a look on our notebook and our filter to go directly to Lab Work, Reunion, Human Practices or Modeling work.<br />
<br />
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{{Team:Paris_Saclay/default_footer}}</div>Maschihttp://2014.igem.org/Team:Paris_Saclay/Ethics/Scientific_Definition_of_Living-BeingTeam:Paris Saclay/Ethics/Scientific Definition of Living-Being2014-10-18T01:45:41Z<p>Maschi: /* Scientific Definition of Living-Being */</p>
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<div>{{Team:Paris_Saclay/ethics_header}}<br />
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<br/><br/><br/><br/><br />
=Scientific Definition of Living-Being=<br />
<br />
Defining “living” has always been challenging. Life is an old notion, complex and very difficult to delimit. With passing time, the definition varies. Indeed, [https://2014.igem.org/Team:Paris_Saclay/Ethics/About_Life_Art_Science History] shows the progression of this notion. But how does this definition change according to various scientific domains? Does life mean the same for a physicist or for a biologist?<br />
<br />
'''From the physicist’s point of view''', the question of life is a current matter. Among the diversity of definitions, we can quote the point of view of Schrödinger for whom life feeds from negative entropy. This definition, which is not the last one but maybe the first one to get the agreement of the majority of the physicists’ community, was permitted by Carnot's work and especially the second principle of thermodynamics, a conclusion of which is that living-beings can maintain a low-constant entropy only because they can export entropy to its environment. ''<br />
However, the question of life is still a concern to physicists because its solving depends not only on a better knowledge of fundamental living mechanisms but also because new technologies such as informatics and synthetic biology are challenging its answer.<br />
<br />
'''According to biologists''', the definition of living-being is based on a number of required properties shared by all living organisms. This definition is not fixed, however, and it is still challenging to get everyone to agree upon a clear and definitive definition. Thus, biology specifies that an entity is alive if it possesses the following properties:<br />
* '''Grow or develop''': the entity has the faculty to grow or to become mature, to produce more or larger cell, to change its structure. At a certain point the entity must reach a mature structure that allows it to reproduce<br />
* '''Be confined''': the entity must have a defined border with the environment<br />
* '''Have a special metabolism''': the entity must be able to feed itself, to transform and stock energy, to use external nutriments from the environment (chemical components from organic or inert matter) or to use stored molecules in its mass, to produce energy, to perform a task and to produce wastes<br />
*'''Respond to environmental changes''': the entity must be able to detect environmental variations (close or far away), and to act in an appropriate way in response. In other words, the entity must respond to stimuli.<br />
* '''Reproduce''': the entity must be able to give birth to similar entities in an autonomous way (sexually or asexually).<br />
<br />
These are some of the properties that are used by biologists to help defining life, but '''this list is not exhaustive''' and other properties can be considered (for example, the maintenance of a dynamic equilibrium between a stable inner milieu and the environment- homeostasis). It shows that '''the definition of life is not set''', as the parameters used to define it can change. Furthermore, certain organisms are considered as belonging to living being although do not satisfy all the properties presented above. For instance, some biologists consider virus as living-being. However, viruses do not use their own metabolism and cannot reproduce themselves without a host cell. Certain symbiotic organisms also do not possess all the above properties, showing once again the limits of the present definitions.<br />
<br />
The development of '''synthetic biology''' in the last decade '''adds another layer of complexity''' to problem of defining life. Indeed, the design of bacterial chassis with minimal genome may involve the loss of mechanisms used by bacteria to evolve (such as conjugation, transposition for example) as these mechanisms are considered as undesirable in a factory bacterium. Such bacteria, enable to evolve, could be considered as not being alive, according the previous definition. Yet, our survey and interviews show that for a large majority of people, these bacteria are still alive, even though some consider that they could be “new living beings”.<br />
<br />
{{Team:Paris_Saclay/default_footer}}</div>Maschihttp://2014.igem.org/Team:Paris_Saclay/Outreach/EventsTeam:Paris Saclay/Outreach/Events2014-10-18T01:43:01Z<p>Maschi: /* Countdown */</p>
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<div>{{Team:Paris_Saclay/outreach_header}}<br />
<br />
=Events=<br />
<br />
<br />
This ethical process is built on questions about life representation and biotechnologies; exchange with people became obvious to progress, especially with general public. Ethics was discussed with some French IGEM teams and we pursued our actions during some public events.<br />
<br />
If you are interested in knowing more about one of this three folowing events, just clik on the name of the event !<br />
<br />
==[https://2014.igem.org/Team:Paris_Saclay/Project/Workshop French Meetup]==<br />
Our team co-hosted the [https://2014.igem.org/Team:Paris_Saclay/Project/Workshop French Meetup] along with Bettencourt and Evry, from 2 August 2014 to 3 August 2014. There were 5 teams in total: Bettencourt, Bordeaux, Evry, Lyon, Saclay. We learned a lot about the different projects and exchanged ideas, notably about ethics during our workshop.<br />
<br />
==[https://2014.igem.org/Team:Paris_Saclay/Outreach/Events/Curiositas Curiositas]==<br />
<br />
[https://2014.igem.org/Team:Paris_Saclay/Outreach/Events/Curiositas Curiositas], held at Orsay, is art-and-science festival that takes place on October the 9th. During a dynamic and constructive debate about bioart and bioethics, our teams can talk with, Marion Laval Jeantet, artist and promoter of the festival, Parisian IGEM teams (Paris Bettencourt and Evry teams) and the audience.<br />
<br />
==[https://2014.igem.org/Team:Paris_Saclay/Outreach/Events/Science_Festival Science Festival]==<br />
<br />
On October the 12th, in the occasion of the [https://2014.igem.org/Team:Paris_Saclay/Outreach/Events/Science_Festival Science Festival] laboratories on Paris-Sud University arrange visits and events for general public. Because it is sometimes difficult to talk about science to children, we tried to didactically approach with experiences and simple visual presentations to illustrate synthetic biology and our project.<br />
<br />
==Meeting with high school students==<br />
<br />
One of the objective of iGEM competition is to promote synthetic biology and to make people aware about the future challenges that it will represent. Thus, we decided to introduce synthetic biology to student from a high school in order to present what synthetic biology exactly is and to collect their feelings about this new science. We were greeted at the high school: “Lycée Jacques Prévert” in the city of Longjumeau near Paris.<br />
<br />
We first describe briefly what a gene is and how it is expressed to clear their head up. Hopefully, they knew perfectly this subject. <br />
We wanted to know what were their first thought when we told about synthetic biology without described it. They usually answered by explaining the meaning of each words: built something with biological organisms. <br />
They were surprised to learn that a bacteria can be used as a machine to produce so many things. Then they realised that maybe we can do whatever we want and it could be dangerous.<br />
<br />
The question of GMOs was raised at this point. They said that GMOs were only profitable for company who made them. They underlined the fact that GMOs were used for a short period and maybe we do not know yet all the effects and it might occur some side effects too. Underlying the fact that GMOs can also produce some medicine, they finally believed that it must be a good tool for high and effective production. <br />
<br />
At the end of the day, they had all the arguments to make their own project when we asked them to write on a paper what they wanted to do with synthetic biology. We collected the paper and realised that they understood what we presented to them.<br />
Here are some of their projects:<br />
*Feed poor countries with specific transformed plant which can survived to the dryness<br />
*Transform human to have wings<br />
*Create a bacteria which can produce paper<br />
*Rainbow human: decrease racism with humans genetically modified to change their skin colour all along the day <br />
<br />
This experience was very helpful to really understand what young students think about this new science. We did not expect them to be so open-minded and it made us confident about the future for synthetic biology.<br />
<br />
<br />
{{Team:Paris_Saclay/default_footer}}</div>Maschihttp://2014.igem.org/Team:Paris_Saclay/OutreachTeam:Paris Saclay/Outreach2014-10-18T01:26:04Z<p>Maschi: /* Events */</p>
<hr />
<div>{{Team:Paris_Saclay/outreach_header}}<br />
<br />
=Outreach=<br />
<br />
==[https://2014.igem.org/Team:Paris_Saclay/Outreach/Events Events]==<br />
<br />
Ethical problems are based on our representation of life and new biotechnologies, hence exchanging with people became obvious to advance toward a generally accepted solution, especially with a non-scientific public. These issues were discussed at the French iGEM Meetup and during some public events.<br />
<br />
<br />
If you are interested in learning more about one of the three following events, just click on the name of the event!<br />
<br />
===[https://2014.igem.org/Team:Paris_Saclay/Project/Workshop French Meetup]===<br />
Our team co-hosted the [https://2014.igem.org/Team:Paris_Saclay/Project/Workshop French Meetup] along with Bettencourt and Evry, from 2 August 2014 to 3 August 2014. There were 5 teams in total: Bettencourt, Bordeaux, Evry, Lyon, Saclay. We learned a lot about the different projects and exchanged ideas, notably about ethics during our workshop.<br />
<br />
===[https://2014.igem.org/Team:Paris_Saclay/Outreach/Events/Curiositas Curiositas]===<br />
<br />
[https://2014.igem.org/Team:Paris_Saclay/Outreach/Events/Curiositas Curiositas], held at Orsay, is art-and-science festival that took place on October 4th to 9th. During a dynamic and constructive debate about bioart and bioethics, our teams talked with Marion Laval-Jeantet, artist and promoter of the festival, the Parisian iGEM teams (Paris Bettencourt and Evry teams) and the audience.<br />
<br />
===[https://2014.igem.org/Team:Paris_Saclay/Outreach/Events/Science_Festival Science Festival]===<br />
<br />
On October the 12th, on the occasion of the French [https://2014.igem.org/Team:Paris_Saclay/Outreach/Events/Science_Festival Science Festival], laboratories of the Paris-Sud University arranged visits and events for general public. Because it is sometimes difficult to talk about science to children, we tried to didactically approach this with experiments and simple visual presentations to illustrate synthetic biology and our project.<br />
<br />
===Meeting with high school students===<br />
<br />
One of the objective of the iGEM competition is to promote synthetic biology and to make people aware about the future challenges that it will represent. Thus, we decided to introduce synthetic biology to students from a high school in order to present what synthetic biology exactly is and to collect their feelings about this new science. We were greeted at the high school: “Lycée Jacques Prévert” in the city of Longjumeau near Paris.<br />
<br />
We first described briefly what a gene is and how it is expressed to clear their heads up. Hopefully, they knew perfectly this subject. <br />
We wanted to know what were their first thought when we told about synthetic biology without described it. They usually answered by explaining the meaning of each words: built something with biological organisms. <br />
They were surprised to learn that a bacteria can be used as a machine to produce so many things. Then they realised that maybe we can do whatever we want and it could be dangerous.<br />
<br />
The question of GMOs was raised at this point. They said that GMOs were only profitable for company who made them. They underlined the fact that GMOs were used for a short period and maybe we do not know yet all the effects and it might occur some side effects too. Underlying the fact that GMOs can also produce some medicine, they finally believed that it must be a good tool for high and effective production. <br />
<br />
At the end of the day, they had all the arguments to make their own project when we asked them to write on a paper what they wanted to do with synthetic biology. We collected the paper and realised that they understood what we presented to them.<br />
Here are some of their projects:<br />
*Feed poor countries with specific transformed plant which can survived to the dryness<br />
*Transform human to have wings<br />
*Create a bacteria which can produce paper<br />
*Rainbow human: decrease racism with humans genetically modified to change their skin colour all along the day <br />
<br />
This experience was very helpful to really understand what young students think about this new science. We did not expect them to be so open-minded and it made us confident about the future for synthetic biology.<br />
<br />
==[https://2014.igem.org/Team:Paris_Saclay/Outreach/Collaborations Collaborations]==<br />
Throughout the project, we exchanged and [https://2014.igem.org/Team:Paris_Saclay/Outreach/Collaborations collaborated] with many iGEM teams.<br />
<br />
{{Team:Paris_Saclay/default_footer}}</div>Maschihttp://2014.igem.org/Team:Paris_Saclay/Project/Inspirations/VanityTeam:Paris Saclay/Project/Inspirations/Vanity2014-10-18T01:25:15Z<p>Maschi: </p>
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<div>{{Team:Paris_Saclay/project_header}}<br />
<br />
=Vanity: Speech about the place of humans in the universe=<br />
<br />
==History==<br />
[[File:Paris_Saclay_Vanite.png|600px|center]]<br />
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<br />
===The Dutch golden age painting===<br />
took place during the XVII century. By this time, the united-provinces were the most prosperous nation of Europe due to their strong economy, art and sciences dynamics.<br />
Although the Dutch golden age painting is part of the baroque tradition, it can be distinguished by its own specificities such as the apparition and the development of several new kinds of pictorials.<br />
<br />
===Influences of the Reform===<br />
North of Europe was facing the protestant reform, which saw idolatry in the cult of Saints and the Virgin. Thus, some leaders of the movement, such as Jean Calvi, did not hesitate to promote the destruction of religious images that were considered to be pegan heresy. In this context, vanities replaced human representations by everyday objects that were used to support the new religious morality. Paintings of “modest” size appeared to support forms of devotion that were far from the splendor of the Catholic Church: the finitude of man, the fragility of its existence and its assets are all themes dear to this troubled time.<br />
<br />
===Antique origins of Vanity…===<br />
Often wrongly associated to a particular type of still-life, Vanity is closer to a meditation theme rather than a type of pictorials. Thus, in Greek antiquity, Vanity was studied in philosophy, literature or painting. The millenary problem of the place of humans in the universe traveled through centuries, taking the form of compositions, assays, “trompe l’oeil” or genre scenes. <br />
In terms of philosophy, Stoics developed a thorough reflexion about the fugacity of terrestrial goods. They distinguish “lent” things (life, material properties, beauty …) to those that are “given” (willingness, reason …). There is no doubt that this heritage has been transmitted by the Humanist movement during the Renaissance, and strongly inspired painting of Vanity in Europe.<br />
<br />
=== … And a biblical origin===<br />
The Vanity theme also originates from an episode of the Bible (Ecclesiates), as shown in this quote: “Vanity of vanities, says Qoheleth, vanity of vanities; all is vanity”. Here, Qoheleth, son of David and king of Jerusalem, asks himself about the meaning of life. “There is no remembrance of past things; nor even for those of the future: they will not be remembered by those who will come later”. Furthermore, the Hebraic word of Vanity literally means “light vapor or ephemeral breathe”. Thus, vanities invite us to meditate about the ephemeral and vain (hence Vanity) nature of human life that faces an unavoidable death.<br />
<br />
====Pieter Claesz, Nature morte avec huitre (1633)====<br />
[[File:Paris_Saclay_Nature.png|300px|left]]<br />
Huile sur bois, 38x53 cm, Staatliche<br />
Junstsammlungen, Kassel<br />
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<br />
===Vanity===<br />
Vanity uses symbols (such as skulls, candles, sandglass …) to highlight the transient character of human life, together with the fragility of terrestrial goods. As we previously mentioned, vanities are part of a long Greek-Roman tradition. However, this theme has been extensively developed during the first half of the XVII century. By evoking the place of humans in the universe, artists emphasize the insignificance of human’s works in regard of God’s ones. The aim is to meditate about the fact that pleasures are useless in regard of death that awaits.<br />
From 1620 to 1630, the dominant representation of still-life remains the « monochrome breakfast ». There is a limited amount of items, positioned under a diagonal light beam in a gray atmosphere. (Pieter Claesz and Willem Claesz Heda). In the second half of the XVII century, colors became more vivid and compositions more complex. For example, this evolution can be seen in Nicolaes Van Veerenael artwork.<br />
<br />
====Nicolaes Van Veerenael, Vanité (1680)====<br />
[[File:Paris_Saclay_Vanity_2.png|300px|left]]<br />
Huile sur bois, 34x46 cm, musée des beaux arts<br />
de Caen<br />
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<br />
==Presentation of the artwork==<br />
This painting was achieved in 1630 in Mauristhuis (La Haye) by a Dutch painter named Pieter Claesz (1597-1661). It represents Vanity- Still life and measures 39.5 × 56 cm (oil on canvas).<br />
<br />
==Description of the painting==<br />
We can see a table on which several distinct objects are dispatched: a book, a burnt candle, a skull, a bone (probably a femur), a pen and an inkwell. The painter used a quasi- monochromatic color palette, conferring an austere and ephemeral atmosphere to the painting, as do the smokes escaping from the candlestick. The painter offers a lateral point of view of the scene, ensuring that the spectator does not crosses the gaze of the skull. The glass is represented lying between the book and the table. The ribbon, to which a key is attached at the tip, seems to be retained on the table only by the presence of the inkwell. Finally, the pen is in equilibrium on a used parchment. All together this staging confers tension to the composition.<br />
<br />
“By evoking the place of Humans in the universe, artists emphasize the insignificance of human’s works in regard of God’s ones.”<br />
<br />
==Analysis of the artwork==<br />
[[File:Paris_Saclay_Vanite.png|600px|center]]<br />
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<br />
===Symbolism===<br />
Typically, this painting is part of the monochrome movement of the beginning of XVII century and thus presents some distinct aspects of Vanity genre:<br />
* bones (skull and femur) directly refer to Death and to the time that goes by,<br />
* the candlestick and the totally burnt candle suggest that all is over,<br />
* the glass suggests the fragility and the futility of human life,<br />
* the book and the pen represent a vanity as they refer to knowledge that diverts humans from God.<br />
* The inkwell, as a terrestrial good, reminds us that every property is ephemeral and vain compared to Death and the divine Word.<br />
<br />
===Composition===<br />
The way the light has been used is remarkable as while shaping objects, it also seems to indicate the spectator a “reading” direction. Indeed, we can see that most items are oriented toward the left and faces the light beam: the glass falls toward the left, the skull “looks” toward the left, the key falls toward the left and the pen tip is oriented toward the left … The painter seems to place the spectator in an observation role. We are also part of the painting as the painter link the spectator to this scene by creating a path.<br />
<br />
Vanity theme is neither about death or life but rather about the transition from one to the other through time. <br />
<br />
Between fascination and repulsion, vanities cultivate the art of ambivalence. Indeed, the interesting paradox of this painting genre consists in that the painting immortalizes the beauty of the world while denigrating it and underlying its fugacity.<br />
<br />
==Why vanity?==<br />
<br />
===From the definition of living being to the place of Humans in the universe===<br />
As we previously mentioned, Vanity evoke the place of humans in the universe, the transience of human existence and the fragility of terrestrial goods. This reflection is linked to our effort of defining the living.<br />
<br />
« For Descartes, there are man-made machines, and life, the machines made by God whose are, in the quote above : ''incomparably better arranged, and adequate to movements more admirable than is any machine of human invention''.»<br />
« The definition of life by entropy is significantly later, but it occurs before the birth of the fathers of thermodynamics and evolution in organisms, Ludvig Boltzmann(1844-1906) and Charles Darwin(1809-1882), in Marie François Xavier Bichat(1771-1802). The latter, sometimes regarded as a vitalist (something Foucault refuses), consider life as mysterious principle which fight against an inert environment. He describe life by : « All the forces that fight against death ».<br />
<br />
{{Team:Paris_Saclay/default_footer}}</div>Maschihttp://2014.igem.org/Team:Paris_Saclay/Project/Inspirations/MagritteTeam:Paris Saclay/Project/Inspirations/Magritte2014-10-18T01:23:53Z<p>Maschi: </p>
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<div>{{Team:Paris_Saclay/project_header}}<br />
<br />
=Rene Magritte=<br />
==Discourse on image==<br />
<br />
[[File:Paris_Saclay_Magritte.png|600px|center]]<br />
<br />
==History==<br />
===Influence of Dadaism===<br />
<br />
Surrealism was born out of the ashes of Dadaism, a provocative movement that broke up with religious, political and artistic values of its time, in reaction to the horrors of World War I (1914-1918). Many Dada writers and artists such as Tzara and Breton found in surrealism a new possibility to express their creative force through poetry.<br />
<br />
===Birth of Surrealism===<br />
The surrealist movement represents a repulsive attitude towards the aftermath of WWI. Indeed after leaving more than 8 million people dead, the “great butchery” instilled a sense of waste and absurdity in young people. <br />
<br />
Surrealism appeared in France around 1924 with the publication of the Surrealist Manifesto by Andre Breton (the de facto leader of the movement), along with other famous comrades such as Louis Aragon, Robert Desnos, Paul Eluard, Rene Magritte, Philippe Soupault, Salvador Dali, and Jacques Prevert. What started as a literary movement soon become involved in other forms of art such as photography and film. The movement spread worldwide during the 1920s and 1930s, gaining much notoriety in the United States as many members emigrated there during the war.<br />
<br />
===Rene Magritte and Surrealism===<br />
In 1923, after discovering a painting by de Chirico, ''The Song of Love'', Magritte embraced Surrealism, realizing that esthetics is trivial compared to ideas. Regarding the work by de Chirico he declared that the painter was “the first to dream of what to paint, not how to paint.” This discovery led Magritte to publish Oesophage, a new magazine. Along with other Belgian artists such as Nouge, Goemans, Andre Souris, and Lecomte, Belgian Surrealism took shape. The first work of the movement, ''The Lost Jockey'', was in 1926.<br />
<br />
===Importance of the relationship between objects, their identification and their representation===<br />
In a long series of works (1928-1966) Magritte showed his intention to show the quasi-invisible relationship between objects and their identification and representation. The series started with ''The Interpretation of Dreams'', ending with a mise en abyme of ''The Two Mysteries''.<br />
<br />
====1927, ''The Interpretation of Dreams''====<br />
[[File:Paris_Saclay_Dreams.png|400px|left]]<br />
Out of the four objects, three show no link with the words beneath them; only the sponge is described “correctly.”<br />
<br />
====1928, ''The Living Mirror''====<br />
[[File:Paris_Saclay_Mirror.png|400px|left]]<br />
In the second work of the series, we observe undefined forms on a black background, identified only by words (personnage éclatant de rire – horizon – armoire – cris d’oiseaux): representation disappears, in stark contrast with the exposed reality of the action, independent of the form given to it.<br />
<br />
====1930: ''The Two Mysteries''====<br />
[[File:Paris_Saclay_Misteries.png|400px|left]]<br />
The last painting in the series depicts an easel with “The Treachery of Images” (which we will discuss later) and a second pipe outside the “painting in a painting.” Is it the model of the pipe in the painting? Is there an intention to render the representation of the pipe in the painting “more real” than the pipe outside the painting? Which one of these two pipes is real then, and which one is just a representation of a pipe?<br />
<br />
==Presentation of the work==<br />
This painting is by the Surrealist painter Rene Magritte (1898-1967). It is called “The Treachery of Images” or “this is not a pipe.” The oil painting on canvas (59x65 cm) was done in 1929. It is currently at the Los Angeles County Museum.<br />
<br />
[[File:Paris_Saclay_Treacheries.png|600px|center]]<br />
<br />
==Description of the work==<br />
Like most of the paintings in the series (this one is #3), ''The Treachery of Images'' represents an everyday object. We see a wooden pipe, painted realistically, on a beige background. Although the pipe is illuminated from the left, we cannot find any shadow: the pipe seems to float in the painting. An enigmatic message reads: ''this is not a pipe.''<br />
<br />
==Analysis==<br />
<br />
“The Treachery of Images” is certainly one of Magritte’s most famous works. If we refer to the title of the painting, the artist’s most obvious intention is to warn us of images which we cannot experience, and which are nothing but representations of the real, tangible, and functional object.<br />
<br />
The message was especially relevant during the 20s and the 30s with the arrival of “talking” movies. No matter how skillful the artist is, no matter how sophisticated the technology is, the image remains a copy, more or less, and does not possess any functionality whatsoever.<br />
<br />
After all, we cannot fill or smoke an image of a pipe. The image cannot bring to the viewer any pleasure or joy related to the object: we cannot experience an image. <br />
<br />
Finally Magritte’s work is never a simple realistic depiction. By looking real, a certain mystery is born out of the normalcy of everyday objects.<br />
<br />
The image painted by Magritte is always an imagined one. His thoughts are highlighted by this mystery created in part by the separation between the reality of words and the truth proposed by an image. <br />
<br />
<br />
''“The form doesn’t interest me, I paint ideas” – Magritte''<br />
<br />
<br />
==Why “The Treachery of Images?”==<br />
===A reminder to not be fooled by images===<br />
As we have seen earlier, Magritte’s work exposes the difference between an object and its representation. We can find similarities with our own project: is a GMO that smells and looks like a lemon an actual lemon? How would it be seen by the general public? Can we accept anything as long as something looks like what we expect of it? Does form come before content? Does our society enjoy these superficial shackles? <br />
<br />
So many questions to which Surrealism proposes the following answer: art should make the invisible visible, and poetry should make the unspeakable speakable. <br />
<br />
===Influence of Magritte on Advertising===<br />
[[File:Paris_Saclay_South.png|200px|left]]<br />
Rene Magritte remains one of the artists of the 20th century with a lasting legacy in commercials. Like Picasso, Warhol, Dali, and Miro, the Belgian Surrealist painter benefits from a notoriety in Europe and owns an easily recognisable and distinct style. His paintings are based on transgression, with the goal of contradicting visual logic. They go well with non-conformist commercials. Let us look at a few examples.<br />
<br />
In our project, we have thought about this duality between real and fake and came up with some short commercials showing the merits of our lemon that isn’t one. <br />
<br />
Directly inspired by ''The Treachery of Images'', this commercial uses Magritte’s work to warn us against “clichés.”<br />
<br />
====Not to Be Reproduced (1937)====<br />
[[File:Paris_Saclay_Reproduce.png|235px|left]]<br />
[[File:Paris_Saclay_Blue.png|425px|left]]<br />
<br />
<html><hr class="cleanhr"/></html><br />
<br />
A man faces a mirror. The troubling nature of the painting comes from the differences in the reflections of the book and the man. The impossible reflection of the man compared to the “normal” one of the book leaves us intrigued.<br />
<br />
An obvious echo to ''Not to Be Reproduced'', this breast cancer campaign tells us to be careful of images, but of living beings instead of objects.<br />
<br />
====The Red Model (1935)====<br />
[[File:Paris_Saclay_Red.png|310px|left]]<br />
[[File:Paris_Saclay_Travel.png|340px|left]]<br />
<br />
<html><hr class="cleanhr"/></html><br />
<br />
The subject of this painting is a pair of shoes. Is this the metamorphosis of human feet reduced to simple shoes, or of shoes elevated to human status?<br />
<br />
Alluding ''The Red Model'', this poster for Canada customs targeting “serious travelers” shows once again the imagination of the Belgian artist.<br />
<br />
{{Team:Paris_Saclay/default_footer}}</div>Maschihttp://2014.igem.org/Template:Team:Paris_Saclay/project_headerTemplate:Team:Paris Saclay/project header2014-10-18T01:21:26Z<p>Maschi: </p>
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<div>{{Team:Paris_Saclay/default_header}}<br />
<html><br />
<img class="branche" src="https://static.igem.org/mediawiki/2014/d/d9/Paris_Saclay_wiki-brancheI.png" style="top:280px;"/><br />
<div id="submenups" class="submenups"><br />
<ul><br />
<li>Project</li><br />
<li><a href="https://2014.igem.org/Team:Paris_Saclay/Project">Overview</a></li><br />
<li><a href="https://2014.igem.org/Team:Paris_Saclay/Project/Inspirations">Inspirations</a></li><br />
<ul><li><a href="https://2014.igem.org/Team:Paris_Saclay/Project/Inspirations/Magritte">Magritte</a></li><br />
<li><a href="https://2014.igem.org/Team:Paris_Saclay/Project/Inspirations/Vanity">Vanity</a></li><br />
</ul><br />
<li><a href="https://2014.igem.org/Team:Paris_Saclay/Project/Fastlemon">Fastlemon</a></li><br />
<li><a href="https://2014.igem.org/Team:Paris_Saclay/Project/Odor-free_ecoli">Remove the bad smell of E.coli</a></li><br />
<li><a href="https://2014.igem.org/Team:Paris_Saclay/Project/Lemon_Scent">Lemon Scent</a></li><br />
<li><a href="https://2014.igem.org/Team:Paris_Saclay/Project/Salicylate_Inducible_System">Lemon Ripening</a></li><br />
<li><a href="https://2014.igem.org/Team:Paris_Saclay/Project/Lemon_Shaping">Lemon Shaping</a></li><br />
<li><a href="https://2014.igem.org/Team:Paris_Saclay/Project/Boston_Installation">Boston Installation</a></li><br />
</ul><br />
</div><br />
</html></div>Maschihttp://2014.igem.org/Team:Paris_Saclay/Project/FastlemonTeam:Paris Saclay/Project/Fastlemon2014-10-18T01:14:00Z<p>Maschi: </p>
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<div>{{Team:Paris_Saclay/project_header}}<br />
<br />
=<FONT color="F68127">FastLemon</FONT>=<br />
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<FONT face="arial"><FONT color="956A12"><FONT size="5pt">'''Have you ever dreamt about having your food magically appeared ?'''<br />
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'''Without going to the supermarket? Without cooking for hours?'''</FONT></FONT><br />
<br />
<FONT size="5pt"><center>'''<FONT color="F68127">Now your dreams come true because YOU have <U>FastLemon</U></font></center></FONT><br />
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<html><div style="width:560px; margin:0 auto;"><iframe width="560" height="315" src="//www.youtube.com/embed/8ayVrNLyxTU" frameborder="0" allowfullscreen></iframe></div></html><br />
<br />
===I bet you're wondering, what is FastLemon?===<br />
<br />
'''FastLemon is the better way to have your food in a flash.''' '''It's like a lemon: the smell is the same, the color is the same and the taste is the same.'''<br />
'''Like a lemon, made by cells linked together by protein, sugar, lipids and others, FASTLEMON contains some ''E. coli'' joined together by agar. So you have exactly the same: cells and sugar. FastLemon is as living as a lemon !'''<br />
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'''After a hard day FASTLEMON is always welcome!'''<br />
<br />
<html><div style="width:560px; margin:0 auto;"><iframe width="420" height="315" src="//www.youtube.com/embed/0L62tj8BM2M" frameborder="0" allowfullscreen></iframe></div></html><br />
<br />
'''This will brighten up your day.'''<br />
<br />
===Why?===<br />
<br />
'''Because our FastLemon has such a wonderful fragrance that you will forget all your problems.'''<br />
<br />
'''In effect FASTLEMON has particular properties that make it very attractive:'''<br />
*'''The bad ''E.coli'' smell was [https://2014.igem.org/Team:Paris_Saclay/Project/Odor-free_ecoli removed]''' <br />
*'''The lemon scent is so captivating because the ''E.coli'' smell was [https://2014.igem.org/Team:Paris_Saclay/Project/Lemon_Scent modified]'''<br />
*'''The apparence is like a lemon: same [https://2014.igem.org/Team:Paris_Saclay/Project/Salicylate_Inducible_System ripening] and same [https://2014.igem.org/Team:Paris_Saclay/Project/Lemon_Shaping shape].'''<br />
<br />
'''So that's why you have to choose FASTLEMON as many consumers already have:'''<br />
<br />
<html><div style="width:560px; margin:0 auto;"><iframe width="560" height="315" src="//www.youtube.com/embed/vyg_71_x-wY" frameborder="0" allowfullscreen></iframe></div></html><br />
<br />
===They tried it!===<br />
<br />
'''Juliette : "''Since I tried FASTLEMON, I can't spend a single day without FASTLEMON''"'''<br />
<br />
'''Leila : "''Since I tried FASTLEMON I have finally time for myself''"'''<br />
<br />
'''Terry : "''Since I tried FASTLEMON my wife cooks better than before''"'''<br />
<br />
'''Romain: "''I tried FASTLEMON the day's end is much more colorful''"'''<br />
<br />
<div style="border: 3px solid" class="center"><br/><FONT size="4pt">'''AND YOU ARE YOU READY TO EAT FASTLEMON ?'''</FONT><br />
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'''Do you think we can believe this advertisement? In your opinion, is it a real or fake lemon?''' '''Living or not living?'''<br />
<br />
'''Does the ad succeed in convincing you to try the product?''' '''Is there any limit with biology?'''<br />
<br />
'''Here are some of the questions we raised in our project.''' [https://2014.igem.org/Team:Paris_Saclay/Ethics/Reflection_about_Artificial_Food Click to see more] !<br/><br/></div><br />
<br />
{{Team:Paris_Saclay/default_footer}}</div>Maschihttp://2014.igem.org/Team:Paris_Saclay/ProjectTeam:Paris Saclay/Project2014-10-18T01:11:01Z<p>Maschi: /* Project */</p>
<hr />
<div>{{Team:Paris_Saclay/project_header}}<br />
=Project=<br />
<br />
IGEM teams use live organism systems to perform specific functions, as mechanics build a machine. Working with life still raises a lot of ethical questions like the representation of life and our team has chosen Bio-Art to explore this subject. To illustrate these questions, we chose an object "made-in-nature", a lemon, and we tried to make an equivalent "made-in-IGEM". This object has the same external properties of a lemon: smell, form and a colour that slowly changes from green to yellow, like a lemon when it ripens.<br />
Moreover we use [https://2014.igem.org/Team:Paris_Saclay/Project/Fastlemon Fastlemon] to raise ethicals issues.<br />
<br />
==Video==<br />
In a collaboration with iGEM Paris-Bettencourt team in our [https://2014.igem.org/Team:Paris_Saclay/Project/Workshop iGEM French Meeting], we made a video to introduce the project.<br />
<html><div style="width:560px; margin:0 auto;"><iframe width="560" height="315" src="//www.youtube.com/embed/oYY6H_yvft0" frameborder="0" allowfullscreen></iframe></div></html><br />
<br />
==Countdown==<br />
This page is under '''Marie''''s responsibility<br />
<br />
* Deadline: 08/oct.<br />
** General introduction of the project.<br />
<br />
* Deadline: 12/oct<br />
** Final review par Sylvie.<br />
<br />
==[https://2014.igem.org/Team:Paris_Saclay/Project/Inspirations Inspirations]==<br />
<br />
== Scientific part==<br />
Our project is based on the construction of a bacterial lemon that will look like lemon but made only of ''Escherichia coli'' modified to produce a lemon scent and to change colour from green to yellow. We will achieve our project by genetically modifying ''Escherichia coli'' to be devoid of any unpleasant odour by deleting the genes involved in ''E. coli'''s odor, by introducing genes for the production of the lemon scent and finally by adding a yellow-blue chromoprotein whose expression is controlled by a tRNA suppressor for the colour switching.<br />
<br />
===[https://2014.igem.org/Team:Paris_Saclay/Project/Odor-free_ecoli Remove the bad smell of ''E. coli'']===<br />
Our lemon should smell like a lemon. Our chassis is ''Escherichia coli'' , a bacterium known to have a foul odor. We thus have to remove the genes involved in this particular phenotype. The ''tnaA'' gene is required for the degradation of tryptophan into indole, the main molecule at the origin of ''E. coli'' smell. We use a phage transduction method to reach our goal.<br />
<br />
===[https://2014.igem.org/Team:Paris_Saclay/Project/Lemon_Scent Lemon Scent]===<br />
The particular odor of lemon is mainly due to 3 monoterpenes: limonene, beta-pinene and geranial. We will clone the 3 monoterpene synthases responsible for the production of these 3 molecules into our bacteria to allow them to produce this fragrance. We will also improve the production of the GPP precursor using a synthetic mevalonate pathway using the construction of the team of T. S. Lee.<br />
<br />
===[https://2014.igem.org/Team:Paris_Saclay/Project/Salicylate_Inducible_System Lemon appearance and Ripening]===<br />
Since vision is one of the most important senses in humans, we want the lemon to look as real as possible. To achieve this, we plan to make our lemon look green or yellow, like a real one. Furthermore, we also want to simulate the ripening process of the lemon by changing its color gradually from green to yellow.<br />
<br />
==Artistic Part==<br />
===[https://2014.igem.org/Team:Paris_Saclay/Project/Lemon_Shaping Lemon Shaping]===<br />
<br />
1st idea<br />
<br />
We basically wanted to build up a lemon tree in which one we would have put lemons we had made. As lemons pass from green to yellow, we would have simulated the season’s cycle.<br />
<br />
===Boston Installation===<br />
<br />
2nd idea<br />
<br />
The idea was to project the shadow of a little sculpture in a screen or a wall: the positioning of the light with respect to the sculpture would have enabled the creation of shadow ten times bigger than the sculpture itself. The aim of this structure would have been to show the gap between a object and its image, between real things and decoys.<br />
<br />
<br />
<br />
3rd idea (chosen for Boston)<br />
<br />
Finally we decided to modify the second idea (for logistic reasons). The element which could this time reveal the gap between an object and its image, between real things and decoys, would be a mirror … <br />
<br />
<br />
====Countdown====<br />
This page is under '''Leïla''''s responsibility<br />
<br />
* Deadline: 08/oct.<br />
** Text<br />
* Deadline: 12/oct<br />
** Final review by Solenne.<br />
<br />
<br />
{{Team:Paris_Saclay/default_footer}}</div>Maschihttp://2014.igem.org/Team:Paris_Saclay/AttributionsTeam:Paris Saclay/Attributions2014-10-18T01:07:07Z<p>Maschi: /* Students */</p>
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<div>{{Team:Paris_Saclay/team_header}}<br />
=Attributions=<br />
==Students==<br />
<br />
*'''Marie Nguyen''' : Experiments (lemon scent, odor Free, salicylate), ethics (interviews, surveys), sponsoring, bibliography.<br />
*'''Mathieu Bousquet''' : Experiments (lemon scent, odor free, salicylate), sponsoring, bibliography.<br />
*'''Floriane Cherrier''' : Events reporter, national collaborations, video, sponsoring, events.<br />
*'''Arnaud Motz''': Experiments (lemon Scent, odor free, salicylate), modeling (fusion protein), sponsoring, bibliography.<br />
*'''Fabio Maschi''' : Web master, experiments (Salicylate), video maker, collaboration, coffee boy.<br />
*'''Terry Mara''': Experiments (lemon scent, odor Free, salicylate), interviews, video, web design.<br />
*'''Eugène Ndiaye''': Experiments(lemon scent), modeling (odor and oxygen diffusion), ethics (surveys).<br />
*'''Maher Ben Khaled''': Experiments (odor free, salicylate), ethics (interviews and surveys), sponsoring, collaborations (Lia Giraud), video, bibliography.<br />
*'''Mathias Vétillard''' : Experiments (lemon scent, odor free, salicylate), sponsoring, ethics, bibliography.<br />
*'''Hoang Vu Vo''' : Experiments (lemon scent, salicylate), ethics (interview).<br />
*'''Leïla Ayachi''' : Art design, web design, art part, video, events, ethics (survey)<br />
*'''Laetitia Doukhan''' : Experiments (lemon scent, salicylate), meetings reporter, web design.<br />
*'''Mélanie Criqui''' : Experiments (lemon scent, salicylate), ethics (surveys and interviews), event, international, collaborations, sponsoring<br />
*'''Romain Sanson''' : Experiments (odor free), art design, video, ethics (surveys and interviews).<br />
*'''Pierre Roux''' : Experiments (lemon scent, salicylate), ethics (About Life, Art and Science), video, modeling (bacterial growth).<br />
*'''Juliette Chevallier''': Experiments (lemon scent, salicylate), ethics (surveys and interviews), sponsoring, events, meetings reporter, video, art design, modeling (bacterial gowth).<br />
*'''Fanny Boulet''' : Web design.<br />
*'''Raphael De St Exupéry''': Ethics (surveys and interviews report).<br />
*'''Rémy Franco''': Ethics (survey), events.<br />
*'''Sean Choe''': Experiments(Lemon Scent, Salicylate), collaborations supervisor, English checking.<br />
<br />
==Advisors & Instructors==<br />
<br />
*'''Alice Devigne''': Advising and supervising experiments<br />
*'''Solenne Ithurbide''': Advising and supervising experiments<br />
*'''Sylvie Lautru''': Advising and supervising experiments<br />
*'''Phillipe Bouloc''': Advising and supervising experiments<br />
*'''Claire Toffano-Nioche''': Advising and modeling supervisor<br />
*'''Olivier Namy''': Advising and supervising experiments<br />
*'''Jean-Luc Pernodet''': Advising and hosting<br />
<br />
<br />
<br />
==Special Thanks==<br />
<br />
<br />
<br />
<br />
In addition to the team, many people have contributed to the project. We would like to thank them. <br />
<br />
We thank Lia Giraud, a very prolific Bio-Artist for guiding us and helping us to define our project. We discussed Bioart and Ethics with her.<br />
We invite you to visit her [http://www.liagiraud.com/ website].<br />
<br />
We thank another Bio-Artist, Marion Laval-Jeantet, who we met on several occasions and gave us valuable advice.<br />
<br />
We also thank [http://agapakis.com/ Christina Agapakis], who came to see us this summer to answer our questions on the Art & Design Track and ethics.<br />
[[File:Paris_Saclay_Meting_Christina.jpg|450px|center|]]<br />
<br />
Lucille, Antoine and Elise, thank you for answering our interviews.<br />
<br />
We thank Philippe Perez for the Fast-Lemon videos that you can go watch on our wiki and Lucille a wonderful actress.<br />
<br />
We thank Naveen and Jia for their proofreading.<br />
<br />
We thank everyone who answered our [https://2014.igem.org/Team:Paris_Saclay/Ethics/Interviews surveys], and all [https://2014.igem.org/Team:Paris_Saclay/Outreach/Collaborations iGEM teams] with whom we collaborated. We thank, [http://ateliers-artistes-belleville.fr/ Therese BICHON], [http://iramis.cea.fr/llb/Phocea/Pisp/visu.php?id=35&uid=%20alexei.grinbaum Alexei GRINBAUM], [http://emmanuelhirsch.fr/ Emmanuel HIRSCH], [http://ifris.org/membre/morgan-meyer/ Morgan MEYER], Partrick SAINT-JEAN and Dominique SCIAMMA.<br />
<br />
Personnal thank : Floriane: Thank you very much to Kenneth Vernick my Boss for letting me use some work hours in this project. <br />
<br />
We thank too [http://www.igmors.u-psud.fr/ the Institut de Génétique et Microbiologie] as host lab and general suport.<br />
<br />
We thank all members who have ponctually or all project long been part of the iGEM team Paris Saclay.(Advisors, Instructors and All students). Thank Alexandre, Alice, Anais, Arnaud, Caroline, Cristina, Claire, Damir, Dimitri, Eugène, Eric, Fabio, Fanny, Floriane, Hoang-Vu,Jean-Luc, Jérémy, Juliette, Laëtitia, Laura, Leila (X2), Lisa, Lucie, Maher, Marie, Mathias, Mathieu, Meghane, Mélanie, Nadia, Olivier, Philippe, Pierre, Raphael (X2), Rémi, Romain, Sean, Solenne, Sylvie, Terry, Xavier. All these people have been essential for the successful completion of this project and this wonderful adventure.<br />
<br />
[[File:Paris_Saclay_iGEMTeamBrainstorming.jpg|450px|center|]]<br />
<br />
<br />
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{{Team:Paris_Saclay/default_footer}}</div>Maschihttp://2014.igem.org/Team:Paris_SaclayTeam:Paris Saclay2014-10-18T00:59:36Z<p>Maschi: </p>
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<div>{{Team:Paris_Saclay/home_header}}<br />
===Project Abstract: This is not a lemon!===<br />
[[File:Paris_Saclay_university.png|200px|right|link=http://www.u-psud.fr/]]<br />
<html><div id="englishversion"></html><br />
Synthetic Biology has the power of potentially changing paradigms of society such as our conception of what living beings are. If we deprive bacteria from all their “unnecessary" functions and have them produce what we need and desire, do these bacteria still possess the status of living organisms or have they become machines?<br />
To raise and explore this question, we adopted an artistic approach. Indeed, we think that '''Bio-Art''' is one of the best ways to reach the citizens and to spark debate and reflection.<br />
<br />
We decided to create a concept organism that would reflect these interrogations. We plan to modify ''Escherichia coli'' in order to produce the '''fragrance''' of a lemon and simulate the '''ripening''' process of a lemon by changing its color gradually from green to yellow. A mixture of bacteria and solid growth medium will be moulded in a lemon '''shape''': it will smell like a lemon, ripe like a lemon and look like a lemon, but "ceci n’est pas un citron” - this is not a lemon… or is it?<br />
<br />
With this project we invite everyone to think about the upcoming opportunities and the necessary ethical limits of designing living beings.<br />
<br />
When iGEM Paris Saclay gives you lemons, break your taboos!<br />
<html></div></html><br />
<html><div id="portugueseversion" class="invisible"></html><br />
A Biologia Sintética tem o poder de mudar os paradigmas da sociedade, como a nossa concepção do que são serem vivos. Se privarmos uma bactéria de todas as suas funções "desnecessárias" e a fizermos produzir o que precisamos e queremos, essa bactéria continuará com um status de organismo vivo ou ela tende a ser uma máquina? Para levantar e explorar essa questão, adotamos uma abordagem artística. De fato, pensamos que '''Bio-Arte''' é uma das melhores maneiras de atingir o grande público e estimar o debate.<br />
<br />
Nós decidimos criar um organismo conceito que refletiria essas interrogações. Nós planejamos modificar ''Escherichia coli'' para que essa produza o '''aroma''' e simule o amadurecimento de um limão, mudando gradualmente sua cor do verde para o amarelo. A mistura de bactérias e meio de cultivo serão moldados em uma forma de limão: ele vai cheirar como um limão, '''amadurecer''' como um limão e ter a '''forma''' de um limão, mas "ceci n'est pas un citron" - isto não é um limão... ou é? <br />
Assim, convidamos todos a pensar sobre as perspectivas e limites éticos de projetas seres vivos.<br />
<br />
Quando iGEM Paris-Saclay lhe der limões, quebre seus tabus!<br />
<br />
<br />
''This abstract was translated to Portuguese by [https://2014.igem.org/Team:Brasil-SP iGEM Brasil-SP] under our [https://2014.igem.org/Team:Paris_Saclay/Outreach/Collaborations collaborations].''<br />
<html></div></html><br />
<html><div id="chineseversion" class="invisible"></html><br />
合成生物学有着改变社会的能力,举个例子,所谓的改变社会,就是我们对于地球上生物的认知。<br />
<br />
如果我们删除了细菌中我们认为不重要的功能,又让他们做我们想让他们做的事情,那么这些细菌是否还保持正常生物体的状态呢?它们是否已经成为了一种生产机器了呢?为了解决这个问题,我们提出了一种艺术性的方法。众所周知,要想让我们的观念能够被大众更好地理解,生物艺术无疑是一种很好的方法。<br />
<br />
我们创建了一种机制能够解决这个问题。我们通过修饰大肠杆菌让它产生一种气味并且模仿柠檬成熟的过程,这个模型需要细菌和它赖以生存的培养基。我们通过五官的感知使这个模型看起来像一个柠檬,但是"ceci n’est pas un citron”——但事实上它并不是一个柠檬,我们只是让它看起来比较像而已。我们希望通过这个实例能够引发大家对生物实验的道德底线的思考。<br />
<br />
<br />
''This abstract was translated to Chinese by [https://2014.igem.org/Team:XMU-China iGEM XMU China] under our [https://2014.igem.org/Team:Paris_Saclay/Outreach/Collaborations collaborations].''<br />
<html></div></html><br />
<html><div id="finnishversion" class="invisible"></html><br />
Synteettisellä biologialla on mahdollisuus muuttaa yhteiskunnan paradigmoja, kuten käsitystämme soluista.<br />
<br />
Jos karsimme bakteereista niiden “tarpeettomat” toiminnot ja valjastamme ne vastaamaan tarpeisiimme, ovatko bakteerit edelleen mielestämme eläviä eliöitä vai onko niistä tullut koneita? Kysymme tämän kysymyksen biotaiteen keinoin, sillä se on yksi parhaita tapoja herättää julkista keskustelua.<br />
<br />
Me luommekonseptiorganismin, joka heijastaa tätä keskustelua. Me muuntelemme ''Escherichia coli'' bakteeria niin, että se tuoksuu ja jäljittelee sitruunan kypsymistä. Teemme bakteerien ja kasvulaustan sekoituksen, joka tuoksuu, kypsyy ja näyttää aivan sitruunalta, mutta "ceci n’est pas un citron” se ei ole sitruuna… vai onko? Tämän avulla haluamme herättää kaikissa ajatuksia elävien organismien suunnitteluun liittyvistä tulevaisuuden mahdollisuuksista ja eettisistä rajoitteista.<br />
<br />
<br />
''This abstract was translated to Finnish by [https://2014.igem.org/Team:Aalto-Helsinki iGEM Aalto-Helsinki] under our [https://2014.igem.org/Team:Paris_Saclay/Outreach/Collaborations collaborations].''<br />
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<html><div id="frenchversion" class="invisible"></html><br />
La biologie synthétique a le pouvoir de changer certains paradigmes de notre société tel que notre conception du statut des êtres vivants.<br />
<br />
Si nous privons des bactéries de leurs fonctions "inutiles" pour qu'elles produisent ce dont nous avons besoin, peut-on toujours les considérer comme des organismes vivants ou sont-elles devenues des machines ?<br />
<br />
Pour aborder cette question, nous avons adopté une approche artistique car nous pensons que le Bio-Art est l'un des meilleurs moyens de susciter des débats avec les citoyens. Nous avons créé un organisme-concept qui pourrait refléter ces interrogations : nous avons modifié ''Escherichia coli'' pour qu'elle produise une odeur de citron et aussi simuler son processus de maturation. Un mélange de bactéries et de milieu de croissance sera coulé dans un moule qui sentira, mûrira et ressemblera à un citron. Mais "ceci n'est pas un citron"- ou cela l'est-il ? Avec notre réalisation nous lançons une invitation à la réflexion sur les futures opportunités et limites éthiques de la conception d’êtres vivants.<br />
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La biologia sintetica potenzialmente ha il potere di cambiare i paradigmi della società come la nostra concezione di ciò che gli esseri viventi sono. Se priviamo i batteri di tutte le loro funzioni "inutili" e facciamo produrre loro ciò di cui abbiamo bisogno e desiderio, questi batteri possiedono ancora lo status di organismi viventi o sono diventati macchine? Per aumentare e esplorare questa domanda, abbiamo adottato un approccio artistico. Infatti, pensiamo che il '''Bio-Art''' è uno dei modi migliori per raggiungere i cittadini e stimolare il dibattito e la riflessione. <br />
<br />
Abbiamo deciso di creare un organismo ideale che riflettesse tali interrogatori. Abbiamo in programma di modificare ''Escherichia coli'' per produrre il '''profumo''' di un limone e simulare il processo di maturazione di un limone cambiando il suo colore gradualmente dal verde al giallo. Una miscela di batteri e crescita solida si modellerà in una '''forma''' di limone: Odorerà come un limone, maturerà come un limone e sembrerà come un limone, ma "ceci n'est pas un citron" - questo non sarà un limone ... o lo è? <br />
<br />
Con questo progetto, invitiamo tutti a pensare alle prossime opportunità e ai limiti etici necessari di progettazione di esseri viventi. <br />
<br />
Quando iGEM Paris Saclay ti da limoni, rompete i vostri tabù!<br />
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사물의 인식 및 개념은 우리가 사는 사회와 문화코드에 상당한 영향을 받습니다. 예를 들어, 옛날에는 어떤 인간은 다른 이의 소유물이었으며 그들의 생사는 주인이 결정하였습니다. 오늘날 이것이 도저히 용납이 안된다면 그것은 ‘인간’의 정의가 그동안 많이 바뀌었기 때문입니다.<br />
합성생물학은 사회의 패러다임을 바꿀 힘을 갖고 있습니다. ‘생물이란 무엇인가?’에 대한 새로운 답도 제시할 수 있습니다. 만약 박테리아에서 ‘필요없는’ 기능들을 제거하고 우리가 원하는 물질을 만들도록 하면, 이 박테리아는 아직도 살아있을까요? 아니면, 하나의 기계로 전락했을까요? 이 질문에 답하기 위해 저희는 예술의 힘을 빌리고자 합니다. 저희는 바이오아트 (BioArt)가 대중에게 다가가고 토론을 싹틔우기에 가장 좋은 방법이라 생각합니다.<br />
이런 생각들을 반영할만한 생명체를 만들고자 합니다. 대장균을 개조하여 레몬의 향과 색을 표현하는 것입니다. 특히, 과일이 익듯이 저희 대장균도 초록색에서 노란색으로 바뀔 것입니다. 레몬 모양의 틀을 이용하여 박테리아가 들어있는 배지도 레몬처럼 보이게 할 계획입니다. 결국 레몬향이 나고 레몬색도 띄고 레몬처럼 보이지만, “ceci n’est pas un citon” – 이것은 레몬이 아닙니다. 그런데 모든점이 레몬과 같다면, 정말 레몬이 아닐까요? <br />
저희 작품을 통해, 합성생물학의 윤리적인 한계에 대해서 다같이 생각해봅시다.<br />
iGEM Paris Saclay와 함께 당신의 터부를 버리세요!<br />
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Synthetische biologie heft het vermogen om de visie van de maatschappij te veranderen zoals on beeld van wat levende wezens zijn.<br />
<br />
Als wij bacterieën ontdoen van hun ‘onnodige’ functies en ze laten produceren wat wij nodig hebben, hebben deze bacteriën dan nog de status van levende organismen, of zijn het machines geworden? Wij hebben een artistieke methode gekozen deze vraag op te werpen omdat Bio-Art de beste manier is om burgers met elkaar in debat te laten gaan.<br />
<br />
We hebben een concept organisme gemaakt dat deze vragen weerspiegelt. We modificeren Escheria coli om de geur van een rijpende citroen te produceren en het rijpingsproces te simuleren. We gieten een mix van bacteriën en groeimedium in een vorm: Zo zal het ruiken, rijpen en er uit zien als een citroen, maar “ceci n’est pas un citron” – dit is geen citroen… Of toch wel? Hiermee nodigen we iedereen uit om na te denken over toekomstige kansen en de ethische grenzen van het ontwerpen van levende wezens.<br />
<br />
<br />
''This abstract was translated to Dutch by [https://2014.igem.org/Team:Groningen iGEM Groningen] under our [https://2014.igem.org/Team:Paris_Saclay/Outreach/Collaborations collaborations].''<br />
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<html><div id="polishversion" class="invisible"></html><br />
Biologia syntetyczna ma moc zmieniania paradygmatów społecznych takich jak nasza koncepcja tego, czym są organizmy żywe. Jeśli pozbawimy bakterie ich „niepotrzebnych” funkcji i zmusimy je do produkcji tego, co nam potrzebne, czy nadal będą one posiadały status organizmów żywych czy staną się maszynami?<br />
<br />
Aby odpowiedzieć na to pytanie, przyjęliśmy podejście artystyczne, jako że Bio-Art jest jednym z najlepszych sposobów wskrzeszenia debaty wśród społeczeństwa. Stworzyliśmy koncepcję organizmu, która odzwierciedlałaby te dochodzenia. Zmodyfikowaliśmy Escherichia coli by wytwarzała zapach i imitowała proces dojrzewania cytryny. Mieszanina bakterii i pożywki odżywczej zostanie ukształtowana: będzie pachnieć, dojrzewać i wyglądać jak cytryna, ale "ceci n’est pas un citron” – to nie cytryna… a może jednak? Wraz z tym pytaniem zapraszamy wszystkich do rozważenia przyszłych okazji i granic etycznych projektowania żywych istot.<br />
<br />
<br />
''This abstract was translated to Polish by [https://2014.igem.org/Team:Warsaw iGEM Warsaw] under our [https://2014.igem.org/Team:Paris_Saclay/Outreach/Collaborations collaborations].''<br />
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<html><div id="norwegianversion" class="invisible"></html><br />
Syntetisk biologi er en mulighet til et paradigmeskifte i samfunnet med tanke på vår oppfatning av hva levende ting er. Hvis vi tar bort bakteriers ”unødvendige” funksjoner og får dem til å produsere ting som vi trenger, vil disse bakteriene fremdeles betraktes som levende organismer, eller har de blitt maskiner? For å sette fokus på denne problemstillingen har vi valgt en kunstnerisk tilnærming ettersom Bio-Kunst er en av de beste måtene å sette i gang en samfunnsdebatt på. Vi har skapt en konsept organisme som reflekterer denne problemstillingen. Vi har modifisert E. coli-bakterier til å produsere lukt og etterlikne modningsprosessen til en sitron. En blanding av bakterier og vekstmedium blandes sammen: Det vil lukte som, modne som og se ut som, en sitron, men ”Ceci n’est pas un citron” – dette er ikke en sitron… eller er det?<br />
Med dette inviterer vi alle til å tenke over hvilke muligheter og etiske grenser vi har når vi designer levende skapninger.<br />
<br />
<br />
''This abstract was translated to Norwegian by [https://2014.igem.org/Team:UiOslo%20Norway iGEM UiOslo_Norway] under our [https://2014.igem.org/Team:Paris_Saclay/Outreach/Collaborations collaborations].''<br />
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<html><div id="spanishversion" class="invisible"></html><br />
La biología sintética tiene el poder de cambiar los paradigmas de la sociedad como nuestra concepción de la definición del ser viviente. <br />
<br />
Si privamos las bacterias de todas sus funciones “inútiles” para que produzcan lo que necesitamos, ¿es que todavía poseen el estatuto de ser vivo o se convirtieron en máquinas?<br />
<br />
Para plantear esta cuestión, adoptamos un enfoque artístico porque creemos que el Bio-Arte es una de las mejores maneras para sensibilizar la población y provocar el debate. Hemos creado un organismo-concepto que podría plantear estas interrogaciones: hemos modificado ''Escherichia coli'' para que produzca el olor del limón y simula su proceso de maturación . Una mezcla de bacterias y de medio de crecimiento sólido será moldeada en un molde de limón: el “limón” olerá como a limón, madurará como un limón y se parecerá a un limón pero “ceci n’est pas un citron” – no es un limón… ¿o lo es?.<br />
Con este proyecto, invitamos a cada uno a pensar acerca de las oportunidades futuras y la necesidad de los límites éticos con respecto al diseño de seres vivos. <br />
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Sentetik biyoloji, yaşayan canlılarla ve bizim açımızla toplum paradigmalarını değiştirme gücüne sahiptir.<br />
Eğer bizler bakterileri “gereksiz” fonksiyonlarından kurtarır ve kendi ihtiyaç duyduğumuz fonksiyonlar eklersek, bu bakteriler hala yaşayan canlı özelliklerine mi sahip olurlar yoksa birer makinaya mı dönüşürler?<br />
<br />
Bu soruyu irdelemek için, Bio-Art adında bir yaklaşım geliştirdik. Dizaynımızın bu sorunu toplumla tartışmak için iyi bir yol olduğunu düşünüyoruz. Bu sorgulamaları yansıtacak bir örnek organizma yarattık. Parfüm üretmek ve bir limonun olgunlaşma sürecini uyarmak için Escherchia coli’yi modifiye ettik. Bir grup bakteri kültürü limon gibi olgunlaşacak, kokacak ve görünecek; ancak “ceci n’est pas un citron”-bu bir limon değil.. ya da öyle mi?<br />
<br />
Bu projeyle, herkesi yaklaşan fırsatlar ve canlıların etik tasarımının sınırları hakkında düşünmeye davet ediyoruz.<br />
<br />
<br />
''This abstract was translated to Turkish by [https://2014.igem.org/Team:METU%20Turkey iGEM METU_Turkey] under our [https://2014.igem.org/Team:Paris_Saclay/Outreach/Collaborations collaborations].''<br />
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<br />
===We let you discover our [https://2014.igem.org/Team:Paris_Saclay/Project/Fastlemon FastLemon]===<br />
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<br />
===We are social!===<br />
Follow our tweets by [http://www.twitter.com/iGEMParisSaclay @iGEMParisSaclay], check our photos on [http://instagram.com/igemparis @iGEMParis] and be updated with our news on [http://www.facebook.com/IgemParisSaclay2012 iGEMParisSaclay]'s wall.<br />
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{{Team:Paris_Saclay/default_footer}}</div>Maschihttp://2014.igem.org/Team:Paris_Saclay/LabworkTeam:Paris Saclay/Labwork2014-10-18T00:52:22Z<p>Maschi: </p>
<hr />
<div>{{Team:Paris_Saclay/labwork_header}}<br />
=Labwork=<br />
==Planning==<br />
We started the labwork in June. The first step was the research project scope and bibliography to ensure the feasibility of our project and to plan all the work we had to do during this wonderful iGEM summer. <br />
In early July we began the experiments, which was completed in the end of September. In order to be effective, we organized ourselves into four groups of summer interns. The groups were formed by all the members of the team, so even students from areas other than biology could cooperate for the project in the laboratory. To ensure the smooth development of the project by all students, the work had a life cycle : <br />
<br />
* Learning: A non biologist had to first learn the basics of biology to understand the lab work.<br />
* Practice: The novice starts to do their experiments with the help of another biology student or an adviser.<br />
* Development: The aforementioned student becomes autonomous and takes initiative.<br />
* Transmission of acquired knowledge: The student, initially oblivious to practices in the lab, can transmit this knowledge at the end of their internship to newcomers.<br />
<br />
===The ''E. coli'' odor-free chassis===<br />
*Culture of MG1655 and MG1655Z1 strains- [https://2014.igem.org/Team:Paris_Saclay/Notebook/June/30 here]<br />
*P1 phage stock preparation for the transduction of the ''Delta-tnaA::Kan'' - [https://2014.igem.org/Team:Paris_Saclay/Notebook/July/2 here]<br />
*P1 phage transduction using the stock prepared on July 2nd to MG1655 and MG1655Z1 strains - [https://2014.igem.org/Team:Paris_Saclay/Notebook/July/3 here]<br />
*Cultures of MG1655 and MG1655Z1 transductants - [https://2014.igem.org/Team:Paris_Saclay/Notebook/July/4 here]<br />
*Transformations test of competent cells MG1655 and MG1655Z1 - [https://2014.igem.org/Team:Paris_Saclay/Notebook/July/10 here]<br />
*Preparation of competent cells E coli, deletion of the antibiotic cassette in the odorless bacteria - [https://2014.igem.org/Team:Paris_Saclay/Notebook/July/11 here]<br />
*Preparation of competent cells E. coli MG1655 and MG1655Z1 transductants - [https://2014.igem.org/Team:Paris_Saclay/Notebook/July/17 here]<br />
*Transformation of supercompetent cells MG1655 and MG1655Z1 transductants with plasmid BT340- [https://2014.igem.org/Team:Paris_Saclay/Notebook/July/18 here]<br />
*Results of the transformation - [https://2014.igem.org/Team:Paris_Saclay/Notebook/July/21 here]<br />
*Preparation and transformation of competent MG1655 and MG1655Z1 transductants - [https://2014.igem.org/Team:Paris_Saclay/Notebook/July/22 here]<br />
*PCR verification of the strains grown - [https://2014.igem.org/Team:Paris_Saclay/Notebook/July/25 here]<br />
*Preparation and Transformation of competent MG1655 and MG1655Z1 with BT340 - [https://2014.igem.org/Team:Paris_Saclay/Notebook/July/28 here]<br />
*PCR of MG1655 and MG1655Z1 with FTR-Apra-F and FTR-Apra-R - [https://2014.igem.org/Team:Paris_Saclay/Notebook/July/29 here]<br />
*Preparation and transformation of electrocompetent MG1655 and MG1655Z1 with plasmid BT340 - [https://2014.igem.org/Team:Paris_Saclay/Notebook/July/30 here]<br />
*Clone isolation by streak of ''Delta-tnaA'' MG1655 and MG1655Z1 on LB dishes - [https://2014.igem.org/Team:Paris_Saclay/Notebook/August/1 here]<br />
*Clone isolation by streak of ''Delta-tnaA'' MG1655 and MG1655Z1 on LB and kan dishes - [https://2014.igem.org/Team:Paris_Saclay/Notebook/August/4 here]<br />
*PCR verification of the final strains ''Delta-tnaA'' MG1655 and MG1655Z1 - [https://2014.igem.org/Team:Paris_Saclay/Notebook/August/5 here]<br />
*Final stock of MG1655 ''Delta-tnaA'' - [https://2014.igem.org/Team:Paris_Saclay/Notebook/August/6 here]<br />
<br />
===The Lemon Scent===<br />
====Preparation====<br />
* Rehydration of BioBricks BBa_J45014, BBa_K517003 - [https://2014.igem.org/Team:Paris_Saclay/Notebook/June/30 here]<br />
* Preparation of electrocompetent DY330 and transformation via pJBEI-6409 - [https://2014.igem.org/Team:Paris_Saclay/Notebook/July/18#Preparation_of_electrocompetent_DY330_and_transformation_via_pJBEI6409 here]<br />
* Extraction of p cola plasmid DNA - [https://2014.igem.org/Team:Paris_Saclay/Notebook/July/22#Extraction_of_p_cola_plasmid_DNA here]<br />
* Extraction and electrophoresis of BBa_K762100 with pSB1C3 - [https://2014.igem.org/Team:Paris_Saclay/Notebook/July/24#Plasmid_DNA_Extraction_&_Electrophoresis here]<br />
* Gel electrophoresis of p cola - [https://2014.igem.org/Team:Paris_Saclay/Notebook/July/24#Gel_electrophoresis_of_p_cola here]<br />
====pPS1====<br />
* PCR targeting with DY330 and pJBEI-6409 - [https://2014.igem.org/Team:Paris_Saclay/Notebook/July/22#PCR_Targeting here]<br />
* Transformation of DY330 with pJBEI-6409 - [https://2014.igem.org/Team:Paris_Saclay/Notebook/July/25#Transformation_of_electrocompetent_cells here]<br />
* Liquid culture of DY330 - [https://2014.igem.org/Team:Paris_Saclay/Notebook/July/28#Liquid_Culture here]<br />
* Transformation of DY330 with pJBEI-6409 - [https://2014.igem.org/Team:Paris_Saclay/Notebook/July/29#Transformation_of_electrocompetent_cells 29th here]<br />
* Culture of DY330 + pJBEI-6409 - [https://2014.igem.org/Team:Paris_Saclay/Notebook/July/31#D_-_Lemon_scent here]<br />
* Transformation of DY330 with pJBEI-6409 - [https://2014.igem.org/Team:Paris_Saclay/Notebook/August/1#New_Transformation_of_electrocompetent_cells here]<br />
* Electroporation of DY330+pJBEI-6409 - [https://2014.igem.org/Team:Paris_Saclay/Notebook/August/4#Electroporation here]<br />
====pPS2-pPS3-pPS4====<br />
*PCR of the differents genes or Biobrick [https://2014.igem.org/Team:Paris_Saclay/Notebook/September/5#PCR_LS_PS_GS_Cad Here]and [https://2014.igem.org/Team:Paris_Saclay/Notebook/September/8#Checking_PCR here]<br />
* Cloning in Topo vector and transformation of competent E.coli [https://2014.igem.org/Team:Paris_Saclay/Notebook/September/8#Cloning here] and [https://2014.igem.org/Team:Paris_Saclay/Notebook/September/11#ligation_of_PS_in_TOPO_vector_and_transformation here]<br />
* Checking of the cloning [https://2014.igem.org/Team:Paris_Saclay/Notebook/September/9 here] and [https://2014.igem.org/Team:Paris_Saclay/Notebook/September/12 here]<br />
* Plasmids extraction [https://2014.igem.org/Team:Paris_Saclay/Notebook/September/10#Plasmid_extraction here]<br />
* Digestion to have the insert [https://2014.igem.org/Team:Paris_Saclay/Notebook/September/11#LS_and_GS_digestion here] and [https://2014.igem.org/Team:Paris_Saclay/Notebook/September/16#Digestion_of_PS_topo_plasmids here]<br />
* Ligation of the insert in the differents plasmids [https://2014.igem.org/Team:Paris_Saclay/Notebook/September/11#LS_and_GS_ligation here] and [https://2014.igem.org/Team:Paris_Saclay/Notebook/September/16#Ligation Here]<br />
* Transformation [https://2014.igem.org/Team:Paris_Saclay/Notebook/September/15#Transformation_with_the_ligation_.28futur_pPS2.2F4.29 Here] and [https://2014.igem.org/Team:Paris_Saclay/Notebook/September/17#transformation here]<br />
* PCR on colony [https://2014.igem.org/Team:Paris_Saclay/Notebook/September/16#PCR_Colony here] and [https://2014.igem.org/Team:Paris_Saclay/Notebook/September/18 here]<br />
* Checking of the insert sens [https://2014.igem.org/Team:Paris_Saclay/Notebook/September/19#PCR_to_check_the_sens_of_the_insert here] and [https://2014.igem.org/Team:Paris_Saclay/Notebook/September/23 here] and [https://2014.igem.org/Team:Paris_Saclay/Notebook/September/24 here]<br />
* Final stock and extraction of pPS3 and pPS4 [https://2014.igem.org/Team:Paris_Saclay/Notebook/September/25 here]<br />
<br />
====pPS5====<br />
*Preparation of the pPS4 plasmid with SalI digestion [https://2014.igem.org/Team:Paris_Saclay/Notebook/September/24#SalI_digestion here]<br />
*Ligation [https://2014.igem.org/Team:Paris_Saclay/Notebook/September/24#SalI_digestion here]<br />
<br />
====PSBIC3====<br />
*Preparation of the plasmids [https://2014.igem.org/Team:Paris_Saclay/Notebook/September/11#Preparation_of_pSCBIC3_plasmid here]<br />
<br />
===Salicylate Inducible Suppressing System===<br />
*Rehydration of BioBricks BBa_J61051 and BBa_K228001- [https://2014.igem.org/Team:Paris_Saclay/Notebook/July/23#The_Suppressing_Salicylate_System 23rd July]<br />
*Transformation of competent E.coli cells - [https://2014.igem.org/Team:Paris_Saclay/Notebook/July/24#Salicylate_Inducible_Suppressing_System 24th July]<br />
*Bacterial culture - [https://2014.igem.org/Team:Paris_Saclay/Notebook/July/25#Salicylate_Inducible_Suppressing_System 25th July]<br />
*Plasmid DNA Purification - [https://2014.igem.org/Team:Paris_Saclay/Notebook/July/28#Salicylate_Inducible_Suppressing_System 28th July]<br />
*Digestion to check & Electrophoresis - [https://2014.igem.org/Team:Paris_Saclay/Notebook/July/28#Salicylate_Inducible_Suppressing_System 28th July]<br />
*Main Digestion of BBa_J61051 and BBa_K228001 - [https://2014.igem.org/Team:Paris_Saclay/Notebook/July/29#Salicylate_Inducible_Suppressing_System 29th July]<br />
*Segregate Process - [https://2014.igem.org/Team:Paris_Saclay/Notebook/July/29#Salicylate_Inducible_Suppressing_System 29th July]<br />
*DNA purification gel agarose - [https://2014.igem.org/Team:Paris_Saclay/Notebook/July/30#Salicylate_Inducible_Suppressing_System 30th July]<br />
*Ligation - [https://2014.igem.org/Team:Paris_Saclay/Notebook/July/30#Salicylate_Inducible_Suppressing_System 30th July]<br />
*Transformation of competent E.coli cells - [https://2014.igem.org/Team:Paris_Saclay/Notebook/July/31#Salicylate_Inducible_Suppressing_System 31st July]<br />
*Liquid Culture - [https://2014.igem.org/Team:Paris_Saclay/Notebook/August/1#Salicylate_Inducible_Suppressing_System 1st August]<br />
*Plasmid DNA Purification - [https://2014.igem.org/Team:Paris_Saclay/Notebook/August/4#Salicylate_Inducible_Suppressing_System 4th August]<br />
*Digestion to check & Electrophoresis - [https://2014.igem.org/Team:Paris_Saclay/Notebook/August/4#Salicylate_Inducible_Suppressing_System 4th August]<br />
*Final Stock '''BBa_K1372000''' - [https://2014.igem.org/Team:Paris_Saclay/Notebook/August/4#Salicylate_Inducible_Suppressing_System 4th August]<br />
*Liquid Culture - [https://2014.igem.org/Team:Paris_Saclay/Notebook/August/5#Salicylate_Inducible_Suppressing_System 5th August]<br />
*Plasmid DNA Purification - [https://2014.igem.org/Team:Paris_Saclay/Notebook/August/6#Salicylate_Inducible_Suppressing_System 6th August]<br />
*Digestion of BBa_K1372000 and BBa_B0015- [https://2014.igem.org/Team:Paris_Saclay/Notebook/August/6#Salicylate_Inducible_Suppressing_System 6th August]<br />
*Segregate Process - [https://2014.igem.org/Team:Paris_Saclay/Notebook/August/6#Salicylate_Inducible_Suppressing_System 6th August]<br />
*DNA purification gel agarose - [https://2014.igem.org/Team:Paris_Saclay/Notebook/August/7#Salicylate_Inducible_Suppressing_System 7th August]<br />
*Ligation - [https://2014.igem.org/Team:Paris_Saclay/Notebook/August/7#Salicylate_Inducible_Suppressing_System 7th August]<br />
*Transformation of competent E.coli cells - [https://2014.igem.org/Team:Paris_Saclay/Notebook/August/8#Salicylate_Inducible_Suppressing_System 8th August]<br />
*Liquid Culture - [https://2014.igem.org/Team:Paris_Saclay/Notebook/August/11#Salicylate_Inducible_Suppressing_System 11th August]<br />
*Plasmid DNA Purification - [https://2014.igem.org/Team:Paris_Saclay/Notebook/August/12#Salicylate_Inducible_Suppressing_System 12th August]<br />
*Digestion to check & Electrophoresis - [https://2014.igem.org/Team:Paris_Saclay/Notebook/August/12#Salicylate_Inducible_Suppressing_System 12th August]<br />
*Final Stock '''BBa_K1372001''' - [https://2014.igem.org/Team:Paris_Saclay/Notebook/August/12#Salicylate_Inducible_Suppressing_System 12th August]<br />
<br />
===Construction of the Fusion Color Chromoprotein===<br />
* Plasmid DNA extraction and electrophoresis - [https://2014.igem.org/Team:Paris_Saclay/Notebook/July/24 24th July]<br />
* PCR of the Chromoprotein - [https://2014.igem.org/Team:Paris_Saclay/Notebook/August/18 18th August]<br />
* PCR Cloning in pGEMT easy - [https://2014.igem.org/Team:Paris_Saclay/Notebook/August/20 20th August]<br />
* Transformation of DH5α with chromoprotein (in pGEMT easy) - [https://2014.igem.org/Team:Paris_Saclay/Notebook/August/21 21st August]<br />
* Plasmid extraction - [https://2014.igem.org/Team:Paris_Saclay/Notebook/August/25 25th August]<br />
* Plasmid extraction - [https://2014.igem.org/Team:Paris_Saclay/Notebook/August/26 26th August]<br />
* Electrophoresis of pGEMT - [https://2014.igem.org/Team:Paris_Saclay/Notebook/August/27 27th August]<br />
* Transformation of DH5α by pSB1C3 - [https://2014.igem.org/Team:Paris_Saclay/Notebook/August/28 28th August]<br />
* Colony replication - [https://2014.igem.org/Team:Paris_Saclay/Notebook/September/1 1st September]<br />
* Liquid culture - [https://2014.igem.org/Team:Paris_Saclay/Notebook/September/2 2nd September]<br />
* Plasmid extraction and gel electrophoresis - [https://2014.igem.org/Team:Paris_Saclay/Notebook/September/3 3rd September]<br />
* PCR - [https://2014.igem.org/Team:Paris_Saclay/Notebook/September/5 5th September]<br />
* Check sequence of the w6 clone - [https://2014.igem.org/Team:Paris_Saclay/Notebook/September/8 8th September]<br />
* PCR Transformation for stock of the w6 clone - [https://2014.igem.org/Team:Paris_Saclay/Notebook/September/15 15th September]<br />
* PCR colony w6 clone, Liquid culture - [https://2014.igem.org/Team:Paris_Saclay/Notebook/September/16 16th September]<br />
<br />
===The Lemon Shaping===<br />
*Concentration Agar Test - [https://2014.igem.org/Team:Paris_Saclay/Notebook/June/30 here]<br />
*Concentration Agar Test - [https://2014.igem.org/Team:Paris_Saclay/Notebook/August/5 here]<br />
*Agar mold test - [https://2014.igem.org/Team:Paris_Saclay/Notebook/August/7 here]<br />
*Agar mold test - [https://2014.igem.org/Team:Paris_Saclay/Notebook/August/8 here]<br />
*Incubation of E. Coli with plasmid FNR RBS AmylCP in LB [https://2014.igem.org/Team:Paris_Saclay/Notebook/August/11 here]<br />
*Coloration of agar [https://2014.igem.org/Team:Paris_Saclay/Notebook/August/12 here]<br />
*Coloration of agar [https://2014.igem.org/Team:Paris_Saclay/Notebook/August/13 here]<br />
*Preparation of M63 medium [https://2014.igem.org/Team:Paris_Saclay/Notebook/August/14 here]<br />
* Incubation of E. Coli with plasmid FNR RBS AmylCP in M63 [https://2014.igem.org/Team:Paris_Saclay/Notebook/August/18#Monday_18th_August here]<br />
*Transformation of odor free E. coli with plasmids coding Fluo Protein [https://2014.igem.org/Team:Paris_Saclay/Notebook/August/27 here]<br />
* Results of Fluorescent Protein [https://2014.igem.org/Team:Paris_Saclay/Notebook/August/29#Friday_29th_August here]<br />
<br />
{{Team:Paris_Saclay/default_footer}}</div>Maschihttp://2014.igem.org/Team:Paris_Saclay/LabworkTeam:Paris Saclay/Labwork2014-10-18T00:20:10Z<p>Maschi: /* Labwork */</p>
<hr />
<div>{{Team:Paris_Saclay/labwork_header}}<br />
=Labwork=<br />
==Planning==<br />
We started the labwork in June. The first step was the research project scope and bibliography to ensure the feasibility of our project and to plan all the work we had to do during this wonderful iGEM summer. <br />
In early July we began the experiments, which was completed in the end of September. In order to be effective, we organized ourselves into four groups of summer interns. The groups were formed by all the members of the team, so even students from areas other than biology could cooperate for the project in the laboratory. To ensure the smooth development of the project by all students, the work had a life cycle : <br />
<br />
* Learning: A non biologist had to first learn the basics of biology to understand the lab work.<br />
* Practice: The novice starts to do their experiments with the help of another biology student or an adviser.<br />
* Development: The aforementioned student becomes autonomous and takes initiative.<br />
* Transmission of acquired knowledge: The student, initially oblivious to practices in the lab, can transmit this knowledge at the end of their internship to newcomers.<br />
<br />
===The ''E. coli'' odor-free chassis===<br />
*Culture of MG1655 and MG1655Z1 strains- [https://2014.igem.org/Team:Paris_Saclay/Notebook/June/30 here]<br />
*P1 phage stock preparation for the transduction of the ''Delta-tnaA::Kan'' - [https://2014.igem.org/Team:Paris_Saclay/Notebook/July/2 here]<br />
*P1 phage transduction using the stock prepared on July 2nd to MG1655 and MG1655Z1 strains - [https://2014.igem.org/Team:Paris_Saclay/Notebook/July/3 here]<br />
*Cultures of MG1655 and MG1655Z1 transductants - [https://2014.igem.org/Team:Paris_Saclay/Notebook/July/4 here]<br />
*Transformations test of competent cells MG1655 and MG1655Z1 - [https://2014.igem.org/Team:Paris_Saclay/Notebook/July/10 here]<br />
*Preparation of competent cells E coli, deletion of the antibiotic cassette in the odorless bacteria - [https://2014.igem.org/Team:Paris_Saclay/Notebook/July/11 here]<br />
*Preparation of competent cells E. coli MG1655 and MG1655Z1 transductants - [https://2014.igem.org/Team:Paris_Saclay/Notebook/July/17 here]<br />
*Transformation of supercompetent cells MG1655 and MG1655Z1 transductants with plasmid BT340- [https://2014.igem.org/Team:Paris_Saclay/Notebook/July/18 here]<br />
*Results of the transformation - [https://2014.igem.org/Team:Paris_Saclay/Notebook/July/21 here]<br />
*Preparation and transformation of competent MG1655 and MG1655Z1 transductants - [https://2014.igem.org/Team:Paris_Saclay/Notebook/July/22 here]<br />
*PCR verification of the strains grown - [https://2014.igem.org/Team:Paris_Saclay/Notebook/July/25 here]<br />
*Preparation and Transformation of competent MG1655 and MG1655Z1 with BT340 - [https://2014.igem.org/Team:Paris_Saclay/Notebook/July/28 here]<br />
*PCR of MG1655 and MG1655Z1 with FTR-Apra-F and FTR-Apra-R - [https://2014.igem.org/Team:Paris_Saclay/Notebook/July/29 here]<br />
*Preparation and transformation of electrocompetent MG1655 and MG1655Z1 with plasmid BT340 - [https://2014.igem.org/Team:Paris_Saclay/Notebook/July/30 here]<br />
*Clone isolation by streak of ''Delta-tnaA'' MG1655 and MG1655Z1 on LB dishes - [https://2014.igem.org/Team:Paris_Saclay/Notebook/August/1 here]<br />
*Clone isolation by streak of ''Delta-tnaA'' MG1655 and MG1655Z1 on LB and kan dishes - [https://2014.igem.org/Team:Paris_Saclay/Notebook/August/4 here]<br />
*PCR verification of the final strains ''Delta-tnaA'' MG1655 and MG1655Z1 - [https://2014.igem.org/Team:Paris_Saclay/Notebook/August/5 here]<br />
*Final stock of MG1655 ''Delta-tnaA'' - [https://2014.igem.org/Team:Paris_Saclay/Notebook/August/6 here]<br />
<br />
===The Lemon Scent===<br />
====Preparation====<br />
* Rehydration of BioBricks BBa_J45014, BBa_K517003 - [https://2014.igem.org/Team:Paris_Saclay/Notebook/June/30 here]<br />
* Preparation of electrocompetent DY330 and transformation via pJBEI-6409 - [https://2014.igem.org/Team:Paris_Saclay/Notebook/July/18#Preparation_of_electrocompetent_DY330_and_transformation_via_pJBEI6409 here]<br />
* Extraction of p cola plasmid DNA - [https://2014.igem.org/Team:Paris_Saclay/Notebook/July/22#Extraction_of_p_cola_plasmid_DNA here]<br />
* Extraction and electrophoresis of BBa_K762100 with pSB1C3 - [https://2014.igem.org/Team:Paris_Saclay/Notebook/July/24#Plasmid_DNA_Extraction_&_Electrophoresis here]<br />
* Gel electrophoresis of p cola - [https://2014.igem.org/Team:Paris_Saclay/Notebook/July/24#Gel_electrophoresis_of_p_cola here]<br />
====pPS1====<br />
* PCR targeting with DY330 and pJBEI-6409 - [https://2014.igem.org/Team:Paris_Saclay/Notebook/July/22#PCR_Targeting here]<br />
* Transformation of DY330 with pJBEI-6409 - [https://2014.igem.org/Team:Paris_Saclay/Notebook/July/25#Transformation_of_electrocompetent_cells here]<br />
* Liquid culture of DY330 - [https://2014.igem.org/Team:Paris_Saclay/Notebook/July/28#Liquid_Culture here]<br />
* Transformation of DY330 with pJBEI-6409 - [https://2014.igem.org/Team:Paris_Saclay/Notebook/July/29#Transformation_of_electrocompetent_cells 29th here]<br />
* Culture of DY330 + pJBEI-6409 - [https://2014.igem.org/Team:Paris_Saclay/Notebook/July/31#D_-_Lemon_scent here]<br />
* Transformation of DY330 with pJBEI-6409 - [https://2014.igem.org/Team:Paris_Saclay/Notebook/August/1#New_Transformation_of_electrocompetent_cells here]<br />
* Electroporation of DY330+pJBEI-6409 - [https://2014.igem.org/Team:Paris_Saclay/Notebook/August/4#Electroporation here]<br />
====pPS2-pPS3-pPS4====<br />
*PCR of the differents genes or Biobrick [https://2014.igem.org/Team:Paris_Saclay/Notebook/September/5#PCR_LS_PS_GS_Cad Here]and [https://2014.igem.org/Team:Paris_Saclay/Notebook/September/8#Checking_PCR here]<br />
* Cloning in Topo vector and transformation of competent E.coli [https://2014.igem.org/Team:Paris_Saclay/Notebook/September/8#Cloning here] and [https://2014.igem.org/Team:Paris_Saclay/Notebook/September/11#ligation_of_PS_in_TOPO_vector_and_transformation here]<br />
* Checking of the cloning [https://2014.igem.org/Team:Paris_Saclay/Notebook/September/9 here] and [https://2014.igem.org/Team:Paris_Saclay/Notebook/September/12 here]<br />
* Plasmids extraction [https://2014.igem.org/Team:Paris_Saclay/Notebook/September/10#Plasmid_extraction here]<br />
* Digestion to have the insert [https://2014.igem.org/Team:Paris_Saclay/Notebook/September/11#LS_and_GS_digestion here] and [https://2014.igem.org/Team:Paris_Saclay/Notebook/September/16#Digestion_of_PS_topo_plasmids here]<br />
* Ligation of the insert in the differents plasmids [https://2014.igem.org/Team:Paris_Saclay/Notebook/September/11#LS_and_GS_ligation here] and [https://2014.igem.org/Team:Paris_Saclay/Notebook/September/16#Ligation Here]<br />
* Transformation [https://2014.igem.org/Team:Paris_Saclay/Notebook/September/15#Transformation_with_the_ligation_.28futur_pPS2.2F4.29 Here] and [https://2014.igem.org/Team:Paris_Saclay/Notebook/September/17#transformation here]<br />
* PCR on colony [https://2014.igem.org/Team:Paris_Saclay/Notebook/September/16#PCR_Colony here] and [https://2014.igem.org/Team:Paris_Saclay/Notebook/September/18 here]<br />
* Checking of the insert sens [https://2014.igem.org/Team:Paris_Saclay/Notebook/September/19#PCR_to_check_the_sens_of_the_insert here] and [https://2014.igem.org/Team:Paris_Saclay/Notebook/September/23 here] and [https://2014.igem.org/Team:Paris_Saclay/Notebook/September/24 here]<br />
* Final stock and extraction of pPS3 and pPS4 [https://2014.igem.org/Team:Paris_Saclay/Notebook/September/25 here]<br />
<br />
====pPS5====<br />
*Preparation of the pPS4 plasmid with SalI digestion [https://2014.igem.org/Team:Paris_Saclay/Notebook/September/24#SalI_digestion here]<br />
*Ligation [https://2014.igem.org/Team:Paris_Saclay/Notebook/September/24#SalI_digestion here]<br />
<br />
====PSBIC3====<br />
*Preparation of the plasmids [https://2014.igem.org/Team:Paris_Saclay/Notebook/September/11#Preparation_of_pSCBIC3_plasmid here]<br />
<br />
===Salicylate Inducible Suppressing System===<br />
*Rehydration of BioBricks BBa_J61051 and BBa_K228001- [https://2014.igem.org/Team:Paris_Saclay/Notebook/July/23#The_Suppressing_Salicylate_System 23rd July]<br />
*Transformation of competent E.coli cells - [https://2014.igem.org/Team:Paris_Saclay/Notebook/July/24#Salicylate_Inducible_Suppressing_System 24th July]<br />
*Bacterial culture - [https://2014.igem.org/Team:Paris_Saclay/Notebook/July/25#Salicylate_Inducible_Suppressing_System 25th July]<br />
*Plasmid DNA Purification - [https://2014.igem.org/Team:Paris_Saclay/Notebook/July/28#Salicylate_Inducible_Suppressing_System 28th July]<br />
*Digestion to check & Electrophoresis - [https://2014.igem.org/Team:Paris_Saclay/Notebook/July/28#Salicylate_Inducible_Suppressing_System 28th July]<br />
*Main Digestion of BBa_J61051 and BBa_K228001 - [https://2014.igem.org/Team:Paris_Saclay/Notebook/July/29#Salicylate_Inducible_Suppressing_System 29th July]<br />
*Segregate Process - [https://2014.igem.org/Team:Paris_Saclay/Notebook/July/29#Salicylate_Inducible_Suppressing_System 29th July]<br />
*DNA purification gel agarose - [https://2014.igem.org/Team:Paris_Saclay/Notebook/July/30#Salicylate_Inducible_Suppressing_System 30th July]<br />
*Ligation - [https://2014.igem.org/Team:Paris_Saclay/Notebook/July/30#Salicylate_Inducible_Suppressing_System 30th July]<br />
*Transformation of competent E.coli cells - [https://2014.igem.org/Team:Paris_Saclay/Notebook/July/31#Salicylate_Inducible_Suppressing_System 31st July]<br />
*Liquid Culture - [https://2014.igem.org/Team:Paris_Saclay/Notebook/August/1#Salicylate_Inducible_Suppressing_System 1st August]<br />
*Plasmid DNA Purification - [https://2014.igem.org/Team:Paris_Saclay/Notebook/August/4#Salicylate_Inducible_Suppressing_System 4th August]<br />
*Digestion to check & Electrophoresis - [https://2014.igem.org/Team:Paris_Saclay/Notebook/August/4#Salicylate_Inducible_Suppressing_System 4th August]<br />
*Final Stock '''BBa_K1372000''' - [https://2014.igem.org/Team:Paris_Saclay/Notebook/August/4#Salicylate_Inducible_Suppressing_System 4th August]<br />
*Liquid Culture - [https://2014.igem.org/Team:Paris_Saclay/Notebook/August/5#Salicylate_Inducible_Suppressing_System 5th August]<br />
*Plasmid DNA Purification - [https://2014.igem.org/Team:Paris_Saclay/Notebook/August/6#Salicylate_Inducible_Suppressing_System 6th August]<br />
*Digestion of BBa_K1372000 and BBa_B0015- [https://2014.igem.org/Team:Paris_Saclay/Notebook/August/6#Salicylate_Inducible_Suppressing_System 6th August]<br />
*Segregate Process - [https://2014.igem.org/Team:Paris_Saclay/Notebook/August/6#Salicylate_Inducible_Suppressing_System 6th August]<br />
*DNA purification gel agarose - [https://2014.igem.org/Team:Paris_Saclay/Notebook/August/7#Salicylate_Inducible_Suppressing_System 7th August]<br />
*Ligation - [https://2014.igem.org/Team:Paris_Saclay/Notebook/August/7#Salicylate_Inducible_Suppressing_System 7th August]<br />
*Transformation of competent E.coli cells - [https://2014.igem.org/Team:Paris_Saclay/Notebook/August/8#Salicylate_Inducible_Suppressing_System 8th August]<br />
*Liquid Culture - [https://2014.igem.org/Team:Paris_Saclay/Notebook/August/11#Salicylate_Inducible_Suppressing_System 11th August]<br />
*Plasmid DNA Purification - [https://2014.igem.org/Team:Paris_Saclay/Notebook/August/12#Salicylate_Inducible_Suppressing_System 12th August]<br />
*Digestion to check & Electrophoresis - [https://2014.igem.org/Team:Paris_Saclay/Notebook/August/12#Salicylate_Inducible_Suppressing_System 12th August]<br />
*Final Stock '''BBa_K1372001''' - [https://2014.igem.org/Team:Paris_Saclay/Notebook/August/12#Salicylate_Inducible_Suppressing_System 12th August]<br />
<br />
===The Lemon Shaping===<br />
*Concentration Agar Test - [https://2014.igem.org/Team:Paris_Saclay/Notebook/June/30 here]<br />
*Concentration Agar Test - [https://2014.igem.org/Team:Paris_Saclay/Notebook/August/5 here]<br />
*Agar mold test - [https://2014.igem.org/Team:Paris_Saclay/Notebook/August/7 here]<br />
*Agar mold test - [https://2014.igem.org/Team:Paris_Saclay/Notebook/August/8 here]<br />
*Incubation of E. Coli with plasmid FNR RBS AmylCP in LB [https://2014.igem.org/Team:Paris_Saclay/Notebook/August/11 here]<br />
*Coloration of agar [https://2014.igem.org/Team:Paris_Saclay/Notebook/August/12 here]<br />
*Coloration of agar [https://2014.igem.org/Team:Paris_Saclay/Notebook/August/13 here]<br />
*Preparation of M63 medium [https://2014.igem.org/Team:Paris_Saclay/Notebook/August/14 here]<br />
* Incubation of E. Coli with plasmid FNR RBS AmylCP in M63 [https://2014.igem.org/Team:Paris_Saclay/Notebook/August/18#Monday_18th_August here]<br />
*Transformation of odor free E. coli with plasmids coding Fluo Protein [https://2014.igem.org/Team:Paris_Saclay/Notebook/August/27 here]<br />
* Results of Fluorescent Protein [https://2014.igem.org/Team:Paris_Saclay/Notebook/August/29#Friday_29th_August here]<br />
<br />
{{Team:Paris_Saclay/default_footer}}</div>Maschihttp://2014.igem.org/Team:Paris_Saclay/LabworkTeam:Paris Saclay/Labwork2014-10-18T00:19:49Z<p>Maschi: /* Planning */</p>
<hr />
<div>{{Team:Paris_Saclay/labwork_header}}<br />
=Labwork=<br />
==Planning==<br />
We started the labwork in June. The first step was the research project scope and bibliography to ensure the feasibility of our project and to plan all the work we had to do during this wonderful iGEM summer. <br />
In early July we began the experiments, which was completed in the end of September. In order to be effective, we organized ourselves into four groups of summer interns. The groups were formed by all the members of the team, so even students from areas other than biology could cooperate for the project in the laboratory. To ensure the smooth development of the project by all students, the work had a life cycle : <br />
<br />
* Learning: A non biologist had to first learn the basics of biology to understand the lab work.<br />
* Practice: The novice starts to do their experiments with the help of another biology student or an adviser.<br />
* Development: The aforementioned student becomes autonomous and takes initiative.<br />
* Transmission of acquired knowledge: The student, initially oblivious to practices in the lab, can transmit this knowledge at the end of their internship to newcomers.<br />
<br />
===The ''E. coli'' odor-free chassis===<br />
*Culture of MG1655 and MG1655Z1 strains- [https://2014.igem.org/Team:Paris_Saclay/Notebook/June/30 here]<br />
*P1 phage stock preparation for the transduction of the ''Delta-tnaA::Kan'' - [https://2014.igem.org/Team:Paris_Saclay/Notebook/July/2 here]<br />
*P1 phage transduction using the stock prepared on July 2nd to MG1655 and MG1655Z1 strains - [https://2014.igem.org/Team:Paris_Saclay/Notebook/July/3 here]<br />
*Cultures of MG1655 and MG1655Z1 transductants - [https://2014.igem.org/Team:Paris_Saclay/Notebook/July/4 here]<br />
*Transformations test of competent cells MG1655 and MG1655Z1 - [https://2014.igem.org/Team:Paris_Saclay/Notebook/July/10 here]<br />
*Preparation of competent cells E coli, deletion of the antibiotic cassette in the odorless bacteria - [https://2014.igem.org/Team:Paris_Saclay/Notebook/July/11 here]<br />
*Preparation of competent cells E. coli MG1655 and MG1655Z1 transductants - [https://2014.igem.org/Team:Paris_Saclay/Notebook/July/17 here]<br />
*Transformation of supercompetent cells MG1655 and MG1655Z1 transductants with plasmid BT340- [https://2014.igem.org/Team:Paris_Saclay/Notebook/July/18 here]<br />
*Results of the transformation - [https://2014.igem.org/Team:Paris_Saclay/Notebook/July/21 here]<br />
*Preparation and transformation of competent MG1655 and MG1655Z1 transductants - [https://2014.igem.org/Team:Paris_Saclay/Notebook/July/22 here]<br />
*PCR verification of the strains grown - [https://2014.igem.org/Team:Paris_Saclay/Notebook/July/25 here]<br />
*Preparation and Transformation of competent MG1655 and MG1655Z1 with BT340 - [https://2014.igem.org/Team:Paris_Saclay/Notebook/July/28 here]<br />
*PCR of MG1655 and MG1655Z1 with FTR-Apra-F and FTR-Apra-R - [https://2014.igem.org/Team:Paris_Saclay/Notebook/July/29 here]<br />
*Preparation and transformation of electrocompetent MG1655 and MG1655Z1 with plasmid BT340 - [https://2014.igem.org/Team:Paris_Saclay/Notebook/July/30 here]<br />
*Clone isolation by streak of ''Delta-tnaA'' MG1655 and MG1655Z1 on LB dishes - [https://2014.igem.org/Team:Paris_Saclay/Notebook/August/1 here]<br />
*Clone isolation by streak of ''Delta-tnaA'' MG1655 and MG1655Z1 on LB and kan dishes - [https://2014.igem.org/Team:Paris_Saclay/Notebook/August/4 here]<br />
*PCR verification of the final strains ''Delta-tnaA'' MG1655 and MG1655Z1 - [https://2014.igem.org/Team:Paris_Saclay/Notebook/August/5 here]<br />
*Final stock of MG1655 ''Delta-tnaA'' - [https://2014.igem.org/Team:Paris_Saclay/Notebook/August/6 here]<br />
<br />
===The Lemon Scent===<br />
====Preparation====<br />
* Rehydration of BioBricks BBa_J45014, BBa_K517003 - [https://2014.igem.org/Team:Paris_Saclay/Notebook/June/30 here]<br />
* Preparation of electrocompetent DY330 and transformation via pJBEI-6409 - [https://2014.igem.org/Team:Paris_Saclay/Notebook/July/18#Preparation_of_electrocompetent_DY330_and_transformation_via_pJBEI6409 here]<br />
* Extraction of p cola plasmid DNA - [https://2014.igem.org/Team:Paris_Saclay/Notebook/July/22#Extraction_of_p_cola_plasmid_DNA here]<br />
* Extraction and electrophoresis of BBa_K762100 with pSB1C3 - [https://2014.igem.org/Team:Paris_Saclay/Notebook/July/24#Plasmid_DNA_Extraction_&_Electrophoresis here]<br />
* Gel electrophoresis of p cola - [https://2014.igem.org/Team:Paris_Saclay/Notebook/July/24#Gel_electrophoresis_of_p_cola here]<br />
====pPS1====<br />
* PCR targeting with DY330 and pJBEI-6409 - [https://2014.igem.org/Team:Paris_Saclay/Notebook/July/22#PCR_Targeting here]<br />
* Transformation of DY330 with pJBEI-6409 - [https://2014.igem.org/Team:Paris_Saclay/Notebook/July/25#Transformation_of_electrocompetent_cells here]<br />
* Liquid culture of DY330 - [https://2014.igem.org/Team:Paris_Saclay/Notebook/July/28#Liquid_Culture here]<br />
* Transformation of DY330 with pJBEI-6409 - [https://2014.igem.org/Team:Paris_Saclay/Notebook/July/29#Transformation_of_electrocompetent_cells 29th here]<br />
* Culture of DY330 + pJBEI-6409 - [https://2014.igem.org/Team:Paris_Saclay/Notebook/July/31#D_-_Lemon_scent here]<br />
* Transformation of DY330 with pJBEI-6409 - [https://2014.igem.org/Team:Paris_Saclay/Notebook/August/1#New_Transformation_of_electrocompetent_cells here]<br />
* Electroporation of DY330+pJBEI-6409 - [https://2014.igem.org/Team:Paris_Saclay/Notebook/August/4#Electroporation here]<br />
====pPS2-pPS3-pPS4====<br />
*PCR of the differents genes or Biobrick [https://2014.igem.org/Team:Paris_Saclay/Notebook/September/5#PCR_LS_PS_GS_Cad Here]and [https://2014.igem.org/Team:Paris_Saclay/Notebook/September/8#Checking_PCR here]<br />
* Cloning in Topo vector and transformation of competent E.coli [https://2014.igem.org/Team:Paris_Saclay/Notebook/September/8#Cloning here] and [https://2014.igem.org/Team:Paris_Saclay/Notebook/September/11#ligation_of_PS_in_TOPO_vector_and_transformation here]<br />
* Checking of the cloning [https://2014.igem.org/Team:Paris_Saclay/Notebook/September/9 here] and [https://2014.igem.org/Team:Paris_Saclay/Notebook/September/12 here]<br />
* Plasmids extraction [https://2014.igem.org/Team:Paris_Saclay/Notebook/September/10#Plasmid_extraction here]<br />
* Digestion to have the insert [https://2014.igem.org/Team:Paris_Saclay/Notebook/September/11#LS_and_GS_digestion here] and [https://2014.igem.org/Team:Paris_Saclay/Notebook/September/16#Digestion_of_PS_topo_plasmids here]<br />
* Ligation of the insert in the differents plasmids [https://2014.igem.org/Team:Paris_Saclay/Notebook/September/11#LS_and_GS_ligation here] and [https://2014.igem.org/Team:Paris_Saclay/Notebook/September/16#Ligation Here]<br />
* Transformation [https://2014.igem.org/Team:Paris_Saclay/Notebook/September/15#Transformation_with_the_ligation_.28futur_pPS2.2F4.29 Here] and [https://2014.igem.org/Team:Paris_Saclay/Notebook/September/17#transformation here]<br />
* PCR on colony [https://2014.igem.org/Team:Paris_Saclay/Notebook/September/16#PCR_Colony here] and [https://2014.igem.org/Team:Paris_Saclay/Notebook/September/18 here]<br />
* Checking of the insert sens [https://2014.igem.org/Team:Paris_Saclay/Notebook/September/19#PCR_to_check_the_sens_of_the_insert here] and [https://2014.igem.org/Team:Paris_Saclay/Notebook/September/23 here] and [https://2014.igem.org/Team:Paris_Saclay/Notebook/September/24 here]<br />
* Final stock and extraction of pPS3 and pPS4 [https://2014.igem.org/Team:Paris_Saclay/Notebook/September/25 here]<br />
<br />
====pPS5====<br />
*Preparation of the pPS4 plasmid with SalI digestion [https://2014.igem.org/Team:Paris_Saclay/Notebook/September/24#SalI_digestion here]<br />
*Ligation [https://2014.igem.org/Team:Paris_Saclay/Notebook/September/24#SalI_digestion here]<br />
<br />
====PSBIC3====<br />
*Preparation of the plasmids [https://2014.igem.org/Team:Paris_Saclay/Notebook/September/11#Preparation_of_pSCBIC3_plasmid here]<br />
<br />
===Salicylate Inducible Suppressing System===<br />
*Rehydration of BioBricks BBa_J61051 and BBa_K228001- [https://2014.igem.org/Team:Paris_Saclay/Notebook/July/23#The_Suppressing_Salicylate_System 23rd July]<br />
*Transformation of competent E.coli cells - [https://2014.igem.org/Team:Paris_Saclay/Notebook/July/24#Salicylate_Inducible_Suppressing_System 24th July]<br />
*Bacterial culture - [https://2014.igem.org/Team:Paris_Saclay/Notebook/July/25#Salicylate_Inducible_Suppressing_System 25th July]<br />
*Plasmid DNA Purification - [https://2014.igem.org/Team:Paris_Saclay/Notebook/July/28#Salicylate_Inducible_Suppressing_System 28th July]<br />
*Digestion to check & Electrophoresis - [https://2014.igem.org/Team:Paris_Saclay/Notebook/July/28#Salicylate_Inducible_Suppressing_System 28th July]<br />
*Main Digestion of BBa_J61051 and BBa_K228001 - [https://2014.igem.org/Team:Paris_Saclay/Notebook/July/29#Salicylate_Inducible_Suppressing_System 29th July]<br />
*Segregate Process - [https://2014.igem.org/Team:Paris_Saclay/Notebook/July/29#Salicylate_Inducible_Suppressing_System 29th July]<br />
*DNA purification gel agarose - [https://2014.igem.org/Team:Paris_Saclay/Notebook/July/30#Salicylate_Inducible_Suppressing_System 30th July]<br />
*Ligation - [https://2014.igem.org/Team:Paris_Saclay/Notebook/July/30#Salicylate_Inducible_Suppressing_System 30th July]<br />
*Transformation of competent E.coli cells - [https://2014.igem.org/Team:Paris_Saclay/Notebook/July/31#Salicylate_Inducible_Suppressing_System 31st July]<br />
*Liquid Culture - [https://2014.igem.org/Team:Paris_Saclay/Notebook/August/1#Salicylate_Inducible_Suppressing_System 1st August]<br />
*Plasmid DNA Purification - [https://2014.igem.org/Team:Paris_Saclay/Notebook/August/4#Salicylate_Inducible_Suppressing_System 4th August]<br />
*Digestion to check & Electrophoresis - [https://2014.igem.org/Team:Paris_Saclay/Notebook/August/4#Salicylate_Inducible_Suppressing_System 4th August]<br />
*Final Stock '''BBa_K1372000''' - [https://2014.igem.org/Team:Paris_Saclay/Notebook/August/4#Salicylate_Inducible_Suppressing_System 4th August]<br />
*Liquid Culture - [https://2014.igem.org/Team:Paris_Saclay/Notebook/August/5#Salicylate_Inducible_Suppressing_System 5th August]<br />
*Plasmid DNA Purification - [https://2014.igem.org/Team:Paris_Saclay/Notebook/August/6#Salicylate_Inducible_Suppressing_System 6th August]<br />
*Digestion of BBa_K1372000 and BBa_B0015- [https://2014.igem.org/Team:Paris_Saclay/Notebook/August/6#Salicylate_Inducible_Suppressing_System 6th August]<br />
*Segregate Process - [https://2014.igem.org/Team:Paris_Saclay/Notebook/August/6#Salicylate_Inducible_Suppressing_System 6th August]<br />
*DNA purification gel agarose - [https://2014.igem.org/Team:Paris_Saclay/Notebook/August/7#Salicylate_Inducible_Suppressing_System 7th August]<br />
*Ligation - [https://2014.igem.org/Team:Paris_Saclay/Notebook/August/7#Salicylate_Inducible_Suppressing_System 7th August]<br />
*Transformation of competent E.coli cells - [https://2014.igem.org/Team:Paris_Saclay/Notebook/August/8#Salicylate_Inducible_Suppressing_System 8th August]<br />
*Liquid Culture - [https://2014.igem.org/Team:Paris_Saclay/Notebook/August/11#Salicylate_Inducible_Suppressing_System 11th August]<br />
*Plasmid DNA Purification - [https://2014.igem.org/Team:Paris_Saclay/Notebook/August/12#Salicylate_Inducible_Suppressing_System 12th August]<br />
*Digestion to check & Electrophoresis - [https://2014.igem.org/Team:Paris_Saclay/Notebook/August/12#Salicylate_Inducible_Suppressing_System 12th August]<br />
*Final Stock '''BBa_K1372001''' - [https://2014.igem.org/Team:Paris_Saclay/Notebook/August/12#Salicylate_Inducible_Suppressing_System 12th August]<br />
<br />
===The Lemon Shaping===<br />
*Concentration Agar Test - [https://2014.igem.org/Team:Paris_Saclay/Notebook/June/30 here]<br />
*Concentration Agar Test - [https://2014.igem.org/Team:Paris_Saclay/Notebook/August/5 here]<br />
*Agar mold test - [https://2014.igem.org/Team:Paris_Saclay/Notebook/August/7 here]<br />
*Agar mold test - [https://2014.igem.org/Team:Paris_Saclay/Notebook/August/8 here]<br />
*Incubation of E. Coli with plasmid FNR RBS AmylCP in LB [https://2014.igem.org/Team:Paris_Saclay/Notebook/August/11 here]<br />
*Coloration of agar [https://2014.igem.org/Team:Paris_Saclay/Notebook/August/12 here]<br />
*Coloration of agar [https://2014.igem.org/Team:Paris_Saclay/Notebook/August/13 here]<br />
*Preparation of M63 medium [https://2014.igem.org/Team:Paris_Saclay/Notebook/August/14 here]<br />
* Incubation of E. Coli with plasmid FNR RBS AmylCP in M63 [https://2014.igem.org/Team:Paris_Saclay/Notebook/August/18#Monday_18th_August here]<br />
*Transformation of odor free E. coli with plasmids coding Fluo Protein [https://2014.igem.org/Team:Paris_Saclay/Notebook/August/27 here]<br />
* Results of Fluorescent Protein [https://2014.igem.org/Team:Paris_Saclay/Notebook/August/29#Friday_29th_August here]<br />
<br />
==Countdown==<br />
This page is under '''Fabio''''s responsibility<br />
<br />
* Deadline: 08/oct.<br />
** Planning of each subproject<br />
* Deadline: 12/oct<br />
** Final review Solenne<br />
<br />
{{Team:Paris_Saclay/default_footer}}</div>Maschihttp://2014.igem.org/Template:Team:Paris_Saclay/home_headerTemplate:Team:Paris Saclay/home header2014-10-18T00:11:54Z<p>Maschi: </p>
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<li><a href="https://2014.igem.org/Team:Paris_Saclay">Home</a></li><br />
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<li><a href="https://igem.org/Team.cgi?year=2014&team_name=Paris_Saclay">Official Profile</a></li><br />
<li><a href="https://2014.igem.org/Team:Paris_Saclay/Attributions">Attributions</a></li><br />
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<li><a href="https://2014.igem.org/Team:Paris_Saclay/Project/Fastlemon">Fastlemon</a></li><br />
<li><a href="https://2014.igem.org/Team:Paris_Saclay/Project/Odor-free_ecoli">Remove the bad smell of E.coli</a></li><br />
<li><a href="https://2014.igem.org/Team:Paris_Saclay/Project/Lemon_Scent">Lemon Scent</a></li><br />
<li><a href="https://2014.igem.org/Team:Paris_Saclay/Project/Salicylate_Inducible_System">Lemon Ripening</a></li><br />
<li><a href="https://2014.igem.org/Team:Paris_Saclay/Project/Lemon_Shaping">Lemon Shaping</a></li><br />
<li><a href="https://2014.igem.org/Team:Paris_Saclay/Project/Boston_Installation">Boston Installation</a></li><br />
</ul><br />
</li><br />
<li><a href="https://2014.igem.org/Team:Paris_Saclay/Modeling">Modeling</a><br />
<ul><br />
<li><a href="https://2014.igem.org/Team:Paris_Saclay/Modeling/oxygen_diffusion">Oxygen Diffusion</a></li><br />
<li><a href="https://2014.igem.org/Team:Paris_Saclay/Modeling/bacterial_Growth">Bacterial Growth</a></li><br />
<li><a href="https://2014.igem.org/Team:Paris_Saclay/Modeling/Fusion_Proteine">Fusion Protein</a></li><br />
<li><a href="https://2014.igem.org/Team:Paris_Saclay/Modeling/odor">Odor</a></li><br />
</ul><br />
</li><br />
<li><a href="https://2014.igem.org/Team:Paris_Saclay/Labwork">Labwork</a><br />
<ul><br />
<li><a href="https://2014.igem.org/Team:Paris_Saclay/Notebook">Notebook</a></li><br />
<li><a href="https://2014.igem.org/Team:Paris_Saclay/Parts">Parts</a></li><br />
<li><a href="https://2014.igem.org/Team:Paris_Saclay/Protocols">Protocols</a></li><br />
<li><a href="https://2014.igem.org/Team:Paris_Saclay/Safety">Safety</a></li><br />
</ul><br />
</li><br />
<li><a href="https://2014.igem.org/Team:Paris_Saclay/Ethics">Ethics</a><br />
<ul><br />
<li><a href="https://2014.igem.org/Team:Paris_Saclay/Ethics/About_Life_Art_Science">Philosophical and Historical Aspects</a></li><br />
<li><a href="https://2014.igem.org/Team:Paris_Saclay/Ethics/Sociological_Cultural_Definition_Living_Being">Sociological and Cultural Aspects</a></li><br />
<li><a href="https://2014.igem.org/Team:Paris_Saclay/Ethics/Scientific Definition of Living-Being">Scientific Aspects</a></li><br />
<li><a href="https://2014.igem.org/Team:Paris_Saclay/Ethics/Interviews">Interviews</a></li><br />
<li><a href="https://2014.igem.org/Team:Paris_Saclay/Ethics/Round_Table">Round Table</a></li><br />
<li><a href="https://2014.igem.org/Team:Paris_Saclay/Ethics/Survey">Survey</a></li><br />
<li><a href="https://2014.igem.org/Team:Paris_Saclay/Ethics/Reflection_about_Artificial_Food">Reflection about Artificial Food</a></li><br />
</ul><br />
</li><br />
<li><a href="https://2014.igem.org/Team:Paris_Saclay/Outreach">Outreach</a><br />
<ul><br />
<li><a href="https://2014.igem.org/Team:Paris_Saclay/Project/Workshop">French Meetup</a><br />
<li><a href="https://2014.igem.org/Team:Paris_Saclay/Outreach/Events/Curiositas">Curiositas</a><br />
<li><a href="https://2014.igem.org/Team:Paris_Saclay/Outreach/Events/Science_Festival">Science Festival</a><br />
<li><a href="https://2014.igem.org/Team:Paris_Saclay/Outreach/Collaborations">Collaborations</a></li><br />
</ul><br />
</li><br />
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</div><br />
<div id="pageContent"></html></div>Maschihttp://2014.igem.org/Template:Team:Paris_Saclay/default_headerTemplate:Team:Paris Saclay/default header2014-10-18T00:08:51Z<p>Maschi: </p>
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<ul><br />
<li><a href="https://2014.igem.org/Team:Paris_Saclay">Home</a></li><br />
<li><a href="https://2014.igem.org/Team:Paris_Saclay/Team">Team</a><br />
<ul><br />
<li><a href="https://igem.org/Team.cgi?year=2014&team_name=Paris_Saclay">Official Profile</a></li><br />
<li><a href="https://2014.igem.org/Team:Paris_Saclay/Attributions">Attributions</a></li><br />
<li><a href="https://2014.igem.org/Team:Paris_Saclay/Team/Sponsor">Sponsors</a></li><br />
</ul><br />
</li><br />
<li><a href="https://2014.igem.org/Team:Paris_Saclay/Project">Project</a><br />
<ul><br />
<li><a href="https://2014.igem.org/Team:Paris_Saclay/Project/Inspirations">Inspirations</a></li><br />
<li><a href="https://2014.igem.org/Team:Paris_Saclay/Project/Fastlemon">Fastlemon</a></li><br />
<li><a href="https://2014.igem.org/Team:Paris_Saclay/Project/Odor-free_ecoli">Remove the bad smell of E.coli</a></li><br />
<li><a href="https://2014.igem.org/Team:Paris_Saclay/Project/Lemon_Scent">Lemon Scent</a></li><br />
<li><a href="https://2014.igem.org/Team:Paris_Saclay/Project/Salicylate_Inducible_System">Lemon Ripening</a></li><br />
<li><a href="https://2014.igem.org/Team:Paris_Saclay/Project/Lemon_Shaping">Lemon Shaping</a></li><br />
<li><a href="https://2014.igem.org/Team:Paris_Saclay/Project/Boston_Installation">Boston Installation</a></li><br />
</ul><br />
</li><br />
<li><a href="https://2014.igem.org/Team:Paris_Saclay/Modeling">Modeling</a><br />
<ul><br />
<li><a href="https://2014.igem.org/Team:Paris_Saclay/Modeling/oxygen_diffusion">Oxygen Diffusion</a></li><br />
<li><a href="https://2014.igem.org/Team:Paris_Saclay/Modeling/bacterial_Growth">Bacterial Growth</a></li><br />
<li><a href="https://2014.igem.org/Team:Paris_Saclay/Modeling/Fusion_Proteine">Fusion Protein</a></li><br />
<li><a href="https://2014.igem.org/Team:Paris_Saclay/Modeling/odor">Odor</a></li><br />
</ul><br />
</li><br />
<li><a href="https://2014.igem.org/Team:Paris_Saclay/Labwork">Labwork</a><br />
<ul><br />
<li><a href="https://2014.igem.org/Team:Paris_Saclay/Notebook">Notebook</a></li><br />
<li><a href="https://2014.igem.org/Team:Paris_Saclay/Parts">Parts</a></li><br />
<li><a href="https://2014.igem.org/Team:Paris_Saclay/Protocols">Protocols</a></li><br />
<li><a href="https://2014.igem.org/Team:Paris_Saclay/Safety">Safety</a></li><br />
</ul><br />
</li><br />
<li><a href="https://2014.igem.org/Team:Paris_Saclay/Ethics">Ethics</a><br />
<ul><br />
<li><a href="https://2014.igem.org/Team:Paris_Saclay/Ethics/About_Life_Art_Science">Philosophical and Historical Aspects</a></li><br />
<li><a href="https://2014.igem.org/Team:Paris_Saclay/Ethics/Sociological_Cultural_Definition_Living_Being">Sociological and Cultural Aspects</a></li><br />
<li><a href="https://2014.igem.org/Team:Paris_Saclay/Ethics/Scientific Definition of Living-Being">Scientific Aspects</a></li><br />
<li><a href="https://2014.igem.org/Team:Paris_Saclay/Ethics/Interviews">Interviews</a></li><br />
<li><a href="https://2014.igem.org/Team:Paris_Saclay/Ethics/Round_Table">Round Table</a></li><br />
<li><a href="https://2014.igem.org/Team:Paris_Saclay/Ethics/Survey">Survey</a></li><br />
<li><a href="https://2014.igem.org/Team:Paris_Saclay/Ethics/Reflection_about_Artificial_Food">Reflection about Artificial Food</a></li><br />
</ul><br />
</li><br />
<li><a href="https://2014.igem.org/Team:Paris_Saclay/Outreach">Outreach</a><br />
<ul><br />
<li><a href="https://2014.igem.org/Team:Paris_Saclay/Project/Workshop">French Meetup</a><br />
<li><a href="https://2014.igem.org/Team:Paris_Saclay/Outreach/Events/Curiositas">Curiositas</a><br />
<li><a href="https://2014.igem.org/Team:Paris_Saclay/Outreach/Events/Science_Festival">Science Festival</a><br />
<li><a href="https://2014.igem.org/Team:Paris_Saclay/Outreach/Collaborations">Collaborations</a></li><br />
</ul><br />
</li><br />
</ul><br />
</div><br />
<div id="pageContent"></html></div>Maschihttp://2014.igem.org/Template:Team:Paris_Saclay/default_headerTemplate:Team:Paris Saclay/default header2014-10-18T00:06:46Z<p>Maschi: </p>
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<div id="pageContent"></html></div>Maschihttp://2014.igem.org/Team:Paris_Saclay/Ethics/Reflection_about_Artificial_FoodTeam:Paris Saclay/Ethics/Reflection about Artificial Food2014-10-17T22:31:05Z<p>Maschi: Created page with "{{Team:Paris_Saclay/ethics_header}} =Reflection about Artificial Food= ==Sub title== Texte bla bla bla {{Team:Paris_Saclay/default_footer}}"</p>
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{{Team:Paris_Saclay/default_footer}}</div>Maschihttp://2014.igem.org/Template:Team:Paris_Saclay/default_headerTemplate:Team:Paris Saclay/default header2014-10-17T22:30:37Z<p>Maschi: </p>
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<div id="pageContent"></html></div>Maschihttp://2014.igem.org/Template:Team:Paris_Saclay/ethics_headerTemplate:Team:Paris Saclay/ethics header2014-10-17T22:30:26Z<p>Maschi: </p>
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</html></div>Maschihttp://2014.igem.org/Template:Team:Paris_Saclay/default_headerTemplate:Team:Paris Saclay/default header2014-10-17T22:28:29Z<p>Maschi: </p>
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<div id="pageContent"></html></div>Maschihttp://2014.igem.org/Template:Team:Paris_Saclay/ethics_headerTemplate:Team:Paris Saclay/ethics header2014-10-17T22:27:40Z<p>Maschi: </p>
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</html></div>Maschihttp://2014.igem.org/Template:Team:Paris_Saclay/default_headerTemplate:Team:Paris Saclay/default header2014-10-17T22:24:58Z<p>Maschi: </p>
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<li><a href="https://2014.igem.org/Team:Paris_Saclay/Project">Project</a><br />
<ul><br />
<li><a href="https://2014.igem.org/Team:Paris_Saclay/Project/Inspirations">Inspirations</a></li><br />
<li><a href="https://2014.igem.org/Team:Paris_Saclay/Project/Fastlemon">Fastlemon</a></li><br />
<li><a href="https://2014.igem.org/Team:Paris_Saclay/Project/Odor-free_ecoli">Remove the bad smell of E.coli</a></li><br />
<li><a href="https://2014.igem.org/Team:Paris_Saclay/Project/Lemon_Scent">Lemon Scent</a></li><br />
<li><a href="https://2014.igem.org/Team:Paris_Saclay/Project/Salicylate_Inducible_System">Lemon Ripening</a></li><br />
<li><a href="https://2014.igem.org/Team:Paris_Saclay/Project/Lemon_Shaping">Lemon Shaping</a></li><br />
<li><a href="https://2014.igem.org/Team:Paris_Saclay/Project/Boston_Installation">Boston Installation</a></li><br />
</ul><br />
</li><br />
<li><a href="https://2014.igem.org/Team:Paris_Saclay/Modeling">Modeling</a><br />
<ul><br />
<li><a href="https://2014.igem.org/Team:Paris_Saclay/Modeling/oxygen_diffusion">Oxygen Diffusion</a></li><br />
<li><a href="https://2014.igem.org/Team:Paris_Saclay/Modeling/bacterial_Growth">Bacterial Growth</a></li><br />
<li><a href="https://2014.igem.org/Team:Paris_Saclay/Modeling/Fusion_Proteine">Fusion Protein</a></li><br />
<li><a href="https://2014.igem.org/Team:Paris_Saclay/Modeling/odor">Odor</a></li><br />
</ul><br />
</li><br />
<li><a href="https://2014.igem.org/Team:Paris_Saclay/Labwork">Labwork</a><br />
<ul><br />
<li><a href="https://2014.igem.org/Team:Paris_Saclay/Notebook">Notebook</a></li><br />
<li><a href="https://2014.igem.org/Team:Paris_Saclay/Parts">Parts</a></li><br />
<li><a href="https://2014.igem.org/Team:Paris_Saclay/Protocols">Protocols</a></li><br />
<li><a href="https://2014.igem.org/Team:Paris_Saclay/Safety">Safety</a></li><br />
</ul><br />
</li><br />
<li><a href="https://2014.igem.org/Team:Paris_Saclay/Ethics">Ethics</a><br />
<ul><br />
<li><a href="https://2014.igem.org/Team:Paris_Saclay/Ethics/About_Life_Art_Science">About Life, Art and Science</a></li><br />
<li><a href="https://2014.igem.org/Team:Paris_Saclay/Ethics/Round_Table">Round Table</a></li><br />
<li><a href="https://2014.igem.org/Team:Paris_Saclay/Ethics/Interviews">Interviews</a></li><br />
<li><a href="https://2014.igem.org/Team:Paris_Saclay/Ethics/Survey">Survey</a></li><br />
</ul><br />
</li><br />
<li><a href="https://2014.igem.org/Team:Paris_Saclay/Outreach">Outreach</a><br />
<ul><br />
<li><a href="https://2014.igem.org/Team:Paris_Saclay/Project/Workshop">French Meetup</a><br />
<li><a href="https://2014.igem.org/Team:Paris_Saclay/Outreach/Events/Curiositas">Curiositas</a><br />
<li><a href="https://2014.igem.org/Team:Paris_Saclay/Outreach/Events/Science_Festival">Science Festival</a><br />
<li><a href="https://2014.igem.org/Team:Paris_Saclay/Outreach/Collaborations">Collaborations</a></li><br />
</ul><br />
</li><br />
</ul><br />
</div><br />
<div id="pageContent"></html></div>Maschihttp://2014.igem.org/Team:Paris_Saclay/Ethics/Scientific_Definition_of_Living-BeingTeam:Paris Saclay/Ethics/Scientific Definition of Living-Being2014-10-17T22:20:49Z<p>Maschi: </p>
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<div>{{Team:Paris_Saclay/ethics_header}}<br />
=Scientific Definition of Living-Being=<br />
==Sub title==<br />
Texte bla bla bla<br />
{{Team:Paris_Saclay/default_footer}}</div>Maschihttp://2014.igem.org/Team:Paris_Saclay/Ethics/Scientific_Definition_of_Living-BeingTeam:Paris Saclay/Ethics/Scientific Definition of Living-Being2014-10-17T22:20:20Z<p>Maschi: Created page with "=Scientific Definition of Living-Being= ==Sub title== Texte bla bla bla {{Team:Paris_Saclay/default_footer}}"</p>
<hr />
<div>=Scientific Definition of Living-Being=<br />
==Sub title==<br />
Texte bla bla bla<br />
{{Team:Paris_Saclay/default_footer}}</div>Maschihttp://2014.igem.org/Template:Team:Paris_Saclay/ethics_headerTemplate:Team:Paris Saclay/ethics header2014-10-17T22:16:31Z<p>Maschi: </p>
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<img class="branche" src="https://static.igem.org/mediawiki/2014/8/85/Paris_Saclay_wiki-brancheIV.png" style="top:190px;"><br />
<div id="submenups" class="submenups"><br />
<ul><br />
<li>Ethics</li><br />
<li><a href="https://2014.igem.org/Team:Paris_Saclay/Ethics">Overview</a></li><br />
<li><a href="https://2014.igem.org/Team:Paris_Saclay/Ethics/About_Life_Art_Science">About Life, Art and Science</a></li><br />
<li><a href="https://2014.igem.org/Team:Paris_Saclay/Ethics/Sociological_Cultural_Definition_Living_Being">Sociological and Cultural definition of living-being</a></li><br />
<li><a href="https://2014.igem.org/Team:Paris_Saclay/Ethics/Round_Table">Round Table</a></li><br />
<li><a href="https://2014.igem.org/Team:Paris_Saclay/Ethics/Interviews">Interviews</a></li><br />
<li><a href="https://2014.igem.org/Team:Paris_Saclay/Ethics/Survey">Survey</a></li><br />
</ul><br />
</div><br />
</html></div>Maschihttp://2014.igem.org/Team:Paris_Saclay/Ethics/Sociological_Cultural_Definition_Living_BeingTeam:Paris Saclay/Ethics/Sociological Cultural Definition Living Being2014-10-17T22:16:18Z<p>Maschi: Created page with "{{Team:Paris_Saclay/ethics_header}} =Sociological and Cultural Definition of Living-Being= ==Sub title== Texte bla bla bla {{Team:Paris_Saclay/default_footer}}"</p>
<hr />
<div>{{Team:Paris_Saclay/ethics_header}}<br />
=Sociological and Cultural Definition of Living-Being=<br />
==Sub title==<br />
Texte bla bla bla<br />
{{Team:Paris_Saclay/default_footer}}</div>Maschihttp://2014.igem.org/Team:Paris_Saclay/EthicsTeam:Paris Saclay/Ethics2014-10-17T22:14:33Z<p>Maschi: </p>
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<div>{{Team:Paris_Saclay/ethics_header}}<br />
=Introduction=<br />
<br />
===Our concerns===<br />
Synthetic Biology in itself is a great source of philosophical and ethical issues. We were concerned about the right to act on nature and especially to modify it. But we discovered that synthetic biology was not created fifteen or ten years ago, it is a very old concept. Indeed, in the last century, our ancestors used crosses between species in order to improve the quality of the food, to increase the yield or also to “create” organisms more resistant to diseases. Thus, the modification of living-being is not a current subject. However, with the technical support in laboratory, synthetic biology can now go further, upsetting already ambiguous definition as living-being. (pas terminé)<br />
<br />
===How our project reflects our concerns===<br />
Our project reflects all the questions we were interesting in. We mimic, imitate the shape, odour, colours of a “natural lemon” with genetically modified bacteria. At first sight, our lemon and a “natural” lemon cannot be distinguished. However, can they be consider as identical? This aspect reveals '''the confused limits between natural and artificial, living-being or non-living being'''. Moreover, after further reflection, we can also emit the hypothesis that the “interior” of the lemon is the same: same taste, same nutritional quality. However, will you estimate it to be identical? '''Would you be ready to eat artificial food created and printed by a 3D printer?''' But is it really artificial? Finally, the use of bioart allow us to raise some questions about the''' concept of art, the link between art and science and to underline question as the right to use living organisms and to modify t hemfor an artistical purpose'''.<br />
<br />
===How did we explore these questions?===<br />
We explored these questions through essay, survey, discussion and also interviews. We tried to ask as many person as we can, coming from very diverse backgrounds (ethicists, scientists, sociologists, artists and designers). The philosophical and historical traits were analysed in an essay. We pursued and improved the analysis of the living-being definition by developing the scientific, sociological and cultural aspects of it. One survey was carried out the international population of iGEMers as subjects in order to collect a maximum of point-of-views and we tried to find trends about the opinion of the iGEM community on these subjects. A debate with French iGEMers was also organised in order to deepen our reflection. Finally, because we chose a lemon to reflect our questionning, we also explored the potential impact of synthetic biology on the food that will be eaten in the future.<br />
<br />
{{Team:Paris_Saclay/default_footer}}</div>Maschihttp://2014.igem.org/File:Paris_Saclay_curiositas2.JPGFile:Paris Saclay curiositas2.JPG2014-10-17T20:47:58Z<p>Maschi: uploaded a new version of &quot;File:Paris Saclay curiositas2.JPG&quot;</p>
<hr />
<div></div>Maschihttp://2014.igem.org/Template:Team:Paris_Saclay/default_headerTemplate:Team:Paris Saclay/default header2014-10-17T20:45:04Z<p>Maschi: </p>
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<li><a href="https://2014.igem.org/Team:Paris_Saclay">Home</a></li><br />
<li><a href="https://2014.igem.org/Team:Paris_Saclay/Team">Team</a><br />
<ul><br />
<li><a href="https://igem.org/Team.cgi?year=2014&team_name=Paris_Saclay">Official Profile</a></li><br />
<li><a href="https://2014.igem.org/Team:Paris_Saclay/Attributions">Attributions</a></li><br />
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<ul><br />
<li><a href="https://2014.igem.org/Team:Paris_Saclay/Project/Inspirations">Inspirations</a></li><br />
<li><a href="https://2014.igem.org/Team:Paris_Saclay/Project/Fastlemon">Fastlemon</a></li><br />
<li><a href="https://2014.igem.org/Team:Paris_Saclay/Project/Odor-free_ecoli">Remove the bad smell of E.coli</a></li><br />
<li><a href="https://2014.igem.org/Team:Paris_Saclay/Project/Lemon_Scent">Lemon Scent</a></li><br />
<li><a href="https://2014.igem.org/Team:Paris_Saclay/Project/Salicylate_Inducible_System">Lemon Ripening</a></li><br />
<li><a href="https://2014.igem.org/Team:Paris_Saclay/Project/Lemon_Shaping">Lemon Shaping</a></li><br />
<li><a href="https://2014.igem.org/Team:Paris_Saclay/Project/Boston_Installation">Boston Installation</a></li><br />
</ul><br />
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<li><a href="https://2014.igem.org/Team:Paris_Saclay/Modeling">Modeling</a><br />
<ul><br />
<li><a href="https://2014.igem.org/Team:Paris_Saclay/Modeling/oxygen_diffusion">Oxygen Diffusion</a></li><br />
<li><a href="https://2014.igem.org/Team:Paris_Saclay/Modeling/bacterial_Growth">Bacterial Growth</a></li><br />
<li><a href="https://2014.igem.org/Team:Paris_Saclay/Modeling/Fusion_Proteine">Fusion Protein</a></li><br />
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<li><a href="https://2014.igem.org/Team:Paris_Saclay/Ethics/Round_Table">Round Table</a></li><br />
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<div id="pageContent"></html></div>Maschihttp://2014.igem.org/Template:Team:Paris_Saclay/default_headerTemplate:Team:Paris Saclay/default header2014-10-17T20:43:18Z<p>Maschi: </p>
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<div id="headerps"><a href="https://2014.igem.org/Team:Paris_Saclay" alt="iGEM Paris Saclay 2014"><img src="https://static.igem.org/mediawiki/2014/9/9b/Paris_Saclay_logo-igem-paris-saclay.png"></a></div><br />
<br />
<div id="menups" class="menups"><br />
<span><img src="https://static.igem.org/mediawiki/2014/9/9b/Paris_Saclay_wiki-menu-left.png"></span><br />
<span><img src="https://static.igem.org/mediawiki/2014/5/5f/Paris_Saclay_wiki-menu-right.png"></span><br />
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<img src="https://static.igem.org/mediawiki/igem.org/6/60/Igemlogo_300px.png" width="55px"><br />
</a><br />
<ul><br />
<li><a href="https://2014.igem.org/Team:Paris_Saclay">Home</a></li><br />
<li><a href="https://2014.igem.org/Team:Paris_Saclay/Team">Team</a><br />
<ul><br />
<li><a href="https://igem.org/Team.cgi?year=2014&team_name=Paris_Saclay">Official Profile</a></li><br />
<li><a href="https://2014.igem.org/Team:Paris_Saclay/Attributions">Attributions</a></li><br />
<li><a href="https://2014.igem.org/Team:Paris_Saclay/Team/Sponsor">Sponsors</a></li><br />
</ul><br />
</li><br />
<li><a href="https://2014.igem.org/Team:Paris_Saclay/Project">Project</a><br />
<ul><br />
<li><a href="https://2014.igem.org/Team:Paris_Saclay/Project/Inspirations">Inspirations</a></li><br />
<li><a href="https://2014.igem.org/Team:Paris_Saclay/Project/Fastlemon">Fastlemon</a></li><br />
<li><a href="https://2014.igem.org/Team:Paris_Saclay/Project/Odor-free_ecoli">Remove the bad smell of E.coli</a></li><br />
<li><a href="https://2014.igem.org/Team:Paris_Saclay/Project/Lemon_Scent">Lemon Scent</a></li><br />
<li><a href="https://2014.igem.org/Team:Paris_Saclay/Project/Salicylate_Inducible_System">Lemon Ripening</a></li><br />
<li><a href="https://2014.igem.org/Team:Paris_Saclay/Project/Lemon_Shaping">Lemon Shaping</a></li><br />
<li><a href="https://2014.igem.org/Team:Paris_Saclay/Project/Boston_Installation">Boston Installation</a></li><br />
</ul><br />
</li><br />
<li><a href="https://2014.igem.org/Team:Paris_Saclay/Modeling">Modeling</a><br />
<ul><br />
<li><a href="https://2014.igem.org/Team:Paris_Saclay/Modeling/oxygen_diffusion">Oxygen Diffusion</a></li><br />
<li><a href="https://2014.igem.org/Team:Paris_Saclay/Modeling/bacterial_Growth">Bacterial Growth</a></li><br />
<li><a href="https://2014.igem.org/Team:Paris_Saclay/Modeling/Fusion_Proteine">Fusion Protein</a></li><br />
<li><a href="https://2014.igem.org/Team:Paris_Saclay/Modeling/odor">Odor</a></li><br />
</ul><br />
</li><br />
<li><a href="https://2014.igem.org/Team:Paris_Saclay/Labwork">Labwork</a><br />
<ul><br />
<li><a href="https://2014.igem.org/Team:Paris_Saclay/Notebook">Notebook</a></li><br />
<li><a href="https://2014.igem.org/Team:Paris_Saclay/Parts">Parts</a></li><br />
<li><a href="https://2014.igem.org/Team:Paris_Saclay/Protocols">Protocols</a></li><br />
<li><a href="https://2014.igem.org/Team:Paris_Saclay/Safety">Safety</a></li><br />
</ul><br />
</li><br />
<li><a href="https://2014.igem.org/Team:Paris_Saclay/Ethics">Ethics</a><br />
<ul><br />
<li><a href="https://2014.igem.org/Team:Paris_Saclay/Ethics/About_Life_Art_Science">About Life, Art and Science</a></li><br />
<li><a href="https://2014.igem.org/Team:Paris_Saclay/Ethics/Round_Table">Round Table</a></li><br />
<li><a href="https://2014.igem.org/Team:Paris_Saclay/Ethics/Interviews">Interviews</a></li><br />
<li><a href="https://2014.igem.org/Team:Paris_Saclay/Ethics/Survey">Survey</a></li><br />
</ul><br />
</li><br />
<li><a href="https://2014.igem.org/Team:Paris_Saclay/Outreach">Outreach</a><br />
<ul><br />
<li><a href="https://2014.igem.org/Team:Paris_Saclay/Project/Workshop">French Meetup</a><br />
<li><a href="https://2014.igem.org/Team:Paris_Saclay/Outreach/Events/Curiositas">Curiositas</a><br />
<li><a href="https://2014.igem.org/Team:Paris_Saclay/Outreach/Events/Science_Festival">Science Festival</a><br />
<li><a href="https://2014.igem.org/Team:Paris_Saclay/Outreach/Collaborations">Collaborations</a></li><br />
</ul><br />
</li><br />
</ul><br />
</div><br />
<div id="pageContent"></html></div>Maschi