User contributions
From 2014.igem.org
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- 13:46, 13 August 2014 (diff | hist) Team:ETH Zurich/lab/protocols (→Preparation of competent E. coli)
- 13:45, 13 August 2014 (diff | hist) Team:ETH Zurich/lab/protocols (→DNA Purification by Centrifugation)
- 13:43, 13 August 2014 (diff | hist) Team:ETH Zurich/lab/protocols (→Preparation of cryostocks)
- 13:43, 13 August 2014 (diff | hist) Team:ETH Zurich/lab/protocols (→Gibson Assembly)
- 13:02, 13 August 2014 (diff | hist) Team:ETH Zurich/lab/protocols (→Preparation of alginate beads)
- 12:51, 13 August 2014 (diff | hist) Team:ETH Zurich/lab/protocols (→Gibson Assembly)
- 12:50, 13 August 2014 (diff | hist) Team:ETH Zurich/lab/protocols (→PCR procol for phusion DNA polymerase)
- 12:49, 13 August 2014 (diff | hist) Team:ETH Zurich/lab/protocols (→Agarose gel electrophoresis)
- 12:42, 13 August 2014 (diff | hist) Team:ETH Zurich/lab/protocols (→Dephosphorylation of 5’-ends of DNA using CIP)
- 10:14, 13 August 2014 (diff | hist) Team:ETH Zurich/lab/protocols (→Quik change mutagenesis)
- 10:11, 13 August 2014 (diff | hist) Team:ETH Zurich/lab/protocols (→Preparation of cryostocks)
- 10:10, 13 August 2014 (diff | hist) Team:ETH Zurich/lab/protocols (→Dephosphorylation of 5’-ends of DNA using CIP)
- 10:08, 13 August 2014 (diff | hist) Team:ETH Zurich/lab/protocols (→Miniprep)
- 08:50, 13 August 2014 (diff | hist) Team:ETH Zurich/lab/protocols (→Agarose gel electrophoresis)
- 08:48, 13 August 2014 (diff | hist) Team:ETH Zurich/lab/protocols (→DNA Purification by Centrifugation)
- 08:47, 13 August 2014 (diff | hist) Team:ETH Zurich/lab/protocols (→DNA Purification by Centrifugation)
- 08:46, 13 August 2014 (diff | hist) Team:ETH Zurich/lab/protocols (→DNA Purification by Centrifugation)
- 08:45, 13 August 2014 (diff | hist) Team:ETH Zurich/lab/protocols (→DNA Purification by Centrifugation)
- 08:44, 13 August 2014 (diff | hist) Team:ETH Zurich/lab/protocols (→DNA Purification by Centrifugation)
- 08:42, 13 August 2014 (diff | hist) Team:ETH Zurich/lab/protocols (→DNA Purification by Centrifugation)
- 08:41, 13 August 2014 (diff | hist) Team:ETH Zurich/lab/protocols (→DNA Purification by Centrifugation)
- 08:35, 13 August 2014 (diff | hist) Team:ETH Zurich/lab/protocols (→Restriction Endonuclease Reactions)
- 08:32, 13 August 2014 (diff | hist) Team:ETH Zurich/lab/protocols (→Dephosphorylation of 5’-ends of DNA using CIP)
- 08:28, 13 August 2014 (diff | hist) Team:ETH Zurich/lab/protocols (→Optimizing Restriction Endonuclease Reactions)
- 08:04, 13 August 2014 (diff | hist) Team:ETH Zurich/lab/protocols (→PCR procol for phusion DNA polymerase)
- 08:00, 13 August 2014 (diff | hist) Team:ETH Zurich/lab/protocols (→Preparation of antibiotics stock)
- 22:47, 12 August 2014 (diff | hist) Team:ETH Zurich/lab/protocols (→PCR procol for phusion DNA polymerase)
- 22:17, 12 August 2014 (diff | hist) Team:ETH Zurich/lab/protocols (→PCR procol for phusion DNA polymerase)
- 22:08, 12 August 2014 (diff | hist) Team:ETH Zurich/lab/protocols (→PCR procol for phusion DNA polymerase)
- 22:02, 12 August 2014 (diff | hist) Team:ETH Zurich/lab/protocols (→Preparation of antibiotics stock)
- 22:01, 12 August 2014 (diff | hist) Team:ETH Zurich/lab/protocols (→Preparation of samples for sequencing at microsynth)
- 21:57, 12 August 2014 (diff | hist) Team:ETH Zurich/lab/protocols (→Miniprep)
- 21:41, 12 August 2014 (diff | hist) Team:ETH Zurich/lab/protocols (→Transformation of competent E. coli)
- 21:39, 12 August 2014 (diff | hist) Team:ETH Zurich/lab/protocols (→Transformation of competent E. coli)
- 21:37, 12 August 2014 (diff | hist) Team:ETH Zurich/lab/protocols (→Preparation of competent E. coli)
- 15:27, 12 August 2014 (diff | hist) Team:ETH Zurich/lab/protocols (→Miniprep)
- 15:25, 12 August 2014 (diff | hist) Team:ETH Zurich/lab/protocols (→Miniprep)
- 15:25, 12 August 2014 (diff | hist) Team:ETH Zurich/lab/protocols (→Precultures for minipreps)
- 15:24, 12 August 2014 (diff | hist) Team:ETH Zurich/lab/protocols (→Transformation of competent E. coli)
- 15:23, 12 August 2014 (diff | hist) Team:ETH Zurich/lab/protocols (→Transformation of competent E. coli)
- 15:23, 12 August 2014 (diff | hist) Team:ETH Zurich/lab/protocols (→Transformation of E. coli)
- 15:17, 12 August 2014 (diff | hist) Team:ETH Zurich/lab/protocols (→Preparation of competent E. coli)
- 15:16, 12 August 2014 (diff | hist) Team:ETH Zurich/lab/protocols (→Preparation of competent E. coli)
- 15:16, 12 August 2014 (diff | hist) Team:ETH Zurich/lab/protocols (→Preparation of competent E. coli)
- 15:11, 12 August 2014 (diff | hist) Team:ETH Zurich/lab/protocols (→Preparation of competent E. coli)
- 15:10, 12 August 2014 (diff | hist) Team:ETH Zurich/lab/protocols (→Preparation of competent E. coli)
- 15:08, 12 August 2014 (diff | hist) Team:ETH Zurich/lab/protocols (→Preparation of competent E. coli)
- 14:58, 12 August 2014 (diff | hist) Team:ETH Zurich/lab/protocols (→Miniprep)
- 14:58, 12 August 2014 (diff | hist) Team:ETH Zurich/lab/protocols (→Miniprep)
- 14:57, 12 August 2014 (diff | hist) Team:ETH Zurich/lab/protocols (→Miniprep)
- 14:54, 12 August 2014 (diff | hist) Team:ETH Zurich/lab/protocols (→Preparation of DNA from iGEM kit)
- 14:53, 12 August 2014 (diff | hist) Team:ETH Zurich/lab/protocols (→Preparation of DNA from iGEM kit)
- 14:52, 12 August 2014 (diff | hist) Team:ETH Zurich/lab/protocols (→Preparation of DNA from iGEM kit)
- 14:49, 12 August 2014 (diff | hist) N Team:ETH Zurich/lab/protocols (Created page with " <html><article class="keyword1 keyword2" id="protocols"></html> ==Preparation of competent E. coli== Addition of 1 mL overnight preculture (see day before) to 100 mL LB-medium...")
- 14:06, 23 July 2014 (diff | hist) Team:ETH Zurich/labblog/20140626 (→Date : Thursday, 5th June (?))
- 14:06, 23 July 2014 (diff | hist) Team:ETH Zurich/labblog/20140626 (→Date : Thursday, 5th June (?))
- 11:33, 23 July 2014 (diff | hist) Team:ETH Zurich/labblog/20140626 (→Date : Thursday, 5th June (?))
- 11:20, 23 July 2014 (diff | hist) Team:ETH Zurich/labblog/20140626 (→Date : Thursday, 5th June (?))
- 20:35, 22 July 2014 (diff | hist) Team:ETH Zurich/labblog/20140626 (→Construction of the rhlR regulator plasmid (piG0042))
- 20:34, 22 July 2014 (diff | hist) N File:F12.png (Fragment fiG0012 was amplified by PCR using the primers oiG0011 and oiG0012 on piG0040 giving the backbone for piG0042) (top)
- 20:31, 22 July 2014 (diff | hist) Team:ETH Zurich/labblog/20140626 (→Construction of the rhlR regulator plasmid (piG0042))
- 20:30, 22 July 2014 (diff | hist) Team:ETH Zurich/labblog/20140626 (→Construction of the rhlR regulator plasmid (piG0042))
- 20:30, 22 July 2014 (diff | hist) Team:ETH Zurich/labblog/20140626 (→Date : Thursday, 5th June (?))
- 20:26, 22 July 2014 (diff | hist) Team:ETH Zurich/labblog/20140626 (→Date : Thursday, 5th June (?))
- 20:15, 22 July 2014 (diff | hist) Team:ETH Zurich/labblog/20140626 (→Date : Thursday, 5th June (?))
- 20:08, 22 July 2014 (diff | hist) Team:ETH Zurich/labblog/20140626 (→Construction of the luxR regulator plasmid (piG0041))
- 20:08, 22 July 2014 (diff | hist) Team:ETH Zurich/labblog/20140626 (→Construction of the lasR regulator plasmid (piG0040))
- 20:05, 22 July 2014 (diff | hist) Team:ETH Zurich/labblog/20140626 (→Construction of the luxR regulator plasmid (piG0041))
- 20:04, 22 July 2014 (diff | hist) Team:ETH Zurich/labblog/20140626 (→Construction of the luxR regulator plasmid (piG0041))
- 20:04, 22 July 2014 (diff | hist) Team:ETH Zurich/labblog/20140626 (→Construction of the luxR regulator plasmid (piG0041))
- 20:03, 22 July 2014 (diff | hist) N File:F11.png (The fragment fiG0011 was amplified by PCR from the backbone and ''lasR'' of piG0040 using the primers oiG0011 and oiG0012) (top)
- 19:50, 22 July 2014 (diff | hist) Team:ETH Zurich/labblog/20140626 (→Construction of the luxR regulator plasmid (piG0041))
- 14:52, 22 July 2014 (diff | hist) Team:ETH Zurich/labblog/20140626 (→Construction of the luxR regulator plasmid (piG0041))
- 14:50, 22 July 2014 (diff | hist) Team:ETH Zurich/labblog/20140626 (→Construction of the lasR regulator plasmid (piG0040))
- 14:50, 22 July 2014 (diff | hist) Team:ETH Zurich/labblog/20140626 (→Construction of the lasR regulator plasmid (piG0040))
- 14:43, 22 July 2014 (diff | hist) Team:ETH Zurich/labblog/20140626 (→Construction of the lasR regulator plasmid (piG0040))
- 14:42, 22 July 2014 (diff | hist) Team:ETH Zurich/labblog/20140626 (→Construction of the luxR regulator plasmid (piG0041))
- 14:41, 22 July 2014 (diff | hist) Team:ETH Zurich/labblog/20140626 (→Construction of the luxR regulator plasmid (piG0041))
- 14:32, 22 July 2014 (diff | hist) N File:PSEVA271.png (Digestion of piG0031 with SpeI and PacI resulted in the fragment fiG0002) (top)
- 14:30, 22 July 2014 (diff | hist) N File:PSEVA181.png (Digestion of piG0034 (pSEVA181) with HindIII and PacI) (top)
- 14:06, 22 July 2014 (diff | hist) N File:F10.png (Fragment fiG0010: PCR amplification of ''rhlI'' from piG0013 using the primers oiG0025 and oiG0026) (top)
- 14:04, 22 July 2014 (diff | hist) N File:F9.png (Fragment fiG0009: PCR amplification of ''lasI'' from piG0012 using the primers oiG0021 and oiG0022) (top)
- 14:02, 22 July 2014 (diff | hist) N File:F8.png (Fragment fiG0008: PCR amplification of ''luxI'' from piG0029 using the primers oiG0017 and oiG0018) (top)
- 14:00, 22 July 2014 (diff | hist) File:F7.png (uploaded a new version of "File:F7.png": Fragment fiG0007: PCR amplification of ''mRFP'' from piG0027 using the primers oiG0015 and oiG0016) (top)
- 13:58, 22 July 2014 (diff | hist) N File:F7.png (Fragment fiG0007: PCR amplification of ''mRFP'' from piG0027 using the primers oiG0015 and oiG0016)
- 13:57, 22 July 2014 (diff | hist) N File:F6.png (Fragment fiG0006: PCR amplification of ''rhlR'' from piG0023 using the primers oiG0009 and oiG0010) (top)
- 13:55, 22 July 2014 (diff | hist) N File:F4.png (Fragment fiG0004: PCR amplification of ''lasR'' from piG0028 using the primers oiG0003 and oiG0004) (top)
- 13:49, 22 July 2014 (diff | hist) N File:20140712 piG0042 GA colony PCRs.jpg (Colony PCR of cells transformed with piG0042, the assembly product of fiG0006 and fiG00012: Samples 1,3,5-8 are positive, 9 is the negative control (piG0034, the backbone used for piG0042, amplified with the same primers) )) (top)
- 13:43, 22 July 2014 (diff | hist) N File:20140710 GA colony PCR.jpg (Colony PCR of cells transformed with piG0040, the assembly product of fiG0001 and fiG0004: Sample 1-8 are positive, 9 is a negative control (piG0034, the backbone used for piG0040, amplified with the same primers)) (top)
- 12:45, 22 July 2014 (diff | hist) Team:ETH Zurich/labblog/20140626 (→Construction of the luxR regulator plasmid (piG0041))
- 12:35, 22 July 2014 (diff | hist) Team:ETH Zurich/labblog/20140626 (→Construction of the lasR regulator plasmid (piG0040))
- 12:31, 22 July 2014 (diff | hist) Team:ETH Zurich/blog
- 15:55, 21 July 2014 (diff | hist) Team:ETH Zurich/blog
- 15:55, 21 July 2014 (diff | hist) Team:ETH Zurich/blog
- 15:52, 21 July 2014 (diff | hist) Team:ETH Zurich/blog
- 15:49, 21 July 2014 (diff | hist) Team:ETH Zurich/labblog/20140626 (→Construction of the luxR regulator plasmid (piG0041))
- 15:48, 21 July 2014 (diff | hist) Team:ETH Zurich/labblog/20140626
- 15:46, 21 July 2014 (diff | hist) Team:ETH Zurich/labblog/20140626
- 15:44, 21 July 2014 (diff | hist) N File:20140710 fiG0005 test PCRs.jpg (blablaba) (top)
- 15:40, 21 July 2014 (diff | hist) N Team:ETH Zurich/labblog/20140626 (Created page with "<html><article class="keyword1 keyword2" id="20140626"></html> == Construction of the regulator plasmids == = Date : Thursday, 5th June (?) = <html><section class="keyword1">...")
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