Harvard BioDesign/30 July 2014

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Ran gel to verify PCR products that we Gibsoned yesterday Backbone (for SynZIPs)- CsgA_48Linker_Flag Backbone (for SpyTag)- CsgA_48Linker

Grow up colonies picked from plates with Gibson- SynZIP#1, #4, #5, SpyTag Picked 3 for each insert 3 from SpyTag 3 from SynZIP#1 3 from SynZIP #4 3 from SynZIP #5 Some of the colonies did not grow up SynZIP#1 (A, B) SpyTag (B) SynZIP#5 (A)

Mini prep colonies

Send plasmids with SpyTag, SynZIPs #1,#4,#5 for sequencing

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