Recycling

TEM Protocol.

In order to verify the synthesis of the CdS nanocrystal in E.coli (Rosetta plysS), the bacteria was harvested by centrifuged in 3000*g for 3 min then suspended in resin and hardened at 60℃ for 16 hours.

Then the hard pellets were cut into 60nm thin slices and the slices were floated on water and deposited on a carbon-coated copper TEM grid. Microscopy was performed with a JEM-1400 microscope at 80-keV electron energy.

Reference.

1. Mareike Hartmann,Marina Berditsch,Jacques Hawecker,Mohammad Fotouhi Ardakani,Dagmar Gerthsen,Anne S. Ulrich1.Damage of the Bacterial Cell Envelope by Antimicrobial Peptides Gramicidin S and PGLa as Revealed by Transmission and Scanning Electron Microscopy Antimicrob Agents Chemother. 2010; 54(8): 3132–3142.
2. Congcong Mi,Yanyan Wang,Jingpu Zhang,Huaiqing Huang,Linru Xu,Shuo Wang,Xuexun Fang,Jin Fang,Chuanbin Mao,Shukun Xu.Biosynthesis and characterization of CdS quantum dots in genetically engineered Escherichia coli. Journal of biotechnology 153(2011) 125-132
3. Ivan Sondi,Branka Salopek-Sondi.Silver nanoparticles as antimicrobial agent: a case study on E. coli as a model for Gram-negative bacteria. Journal of Colloid and Interface Science 275 (2004) 177–182