Team:Hong Kong-CUHK/safety template.html
From 2014.igem.org
Your Training
Have your team members received any safety training yet?
Yes, we have already received safety training.
Please briefly describe the topics that you learned about (or will learn about) in your safety training.
We have received Biosafety courses on:- Safety laws and policy
- Fire safety and emergency procedures
- Electrical safety
- Safety information resources including MSDS
- Chemical handling requirements and waste disposal procedures
- Use of personal protective equipment
- Practicing safe science
- Use of fume cupboards
- Principle of Biological Safety
- Risk Group and Biosafety Level
- Laboratory Practices
- Bioaerosol
- Biosafety Cabinet
- Sterilization
- Biowastes
- Genetically Manipulated Organisms
- Risk-Grouping of Micro-organisms by Type
- Biosafety Levels
- Abbreviated Laboratory Biosafety Level Criteria
- Handling Blood and Other "Products" of Human Origin
- Biosafety Cabinets
- Hazards from "Microbial" Aerosols
- Work with Recombinant DNA
- Treatment of Biological Waste and Spill Cleanup
- Autoclave Safety and Disinfection
- Clinical Waste Disposal
Please give a link to the laboratory safety training requirements of your institution (college, university, community lab, etc). Or, if you cannot give a link, briefly describe the requirements.
http://www.cuhk.edu.hk/useo/so/training.htmlYour Local Rules and Regulations
Who is responsible for biological safety at your institution? (You might have an Institutional Biosafety Committee, an Office of Environmental Health and Safety, a single Biosafety Officer, or some other arrangement.) Have you discussed your project with them? Describe any concerns they raised, and any changes you made in your project based on your discussion.
Mr. YIP Sung Tat from the University Safety Office of the Chinese University of Hong Kong is one of the persons responsible for biological safety at our institution.The University Safety Office have done a regular safety check of the lab on 7/3/2014 and the rectification has been carried out by department.
We discussed our project with university safety office every year, for evaluation of the project safety issue.
What are the biosafety guidelines of your institution? Please give a link to these guidelines, or briefly describe them if you cannot give a link.
http://www.cuhk.edu.hk/useo/safety/ko_download/safetypolicy.pdfIn your country, what are the regulations that govern biosafety in research laboratories? Please give a link to these regulations, or briefly describe them if you cannot give a link.
http://www.chp.gov.hk/files/pdf/guidelines_on_biosafety_in_the_clinical_laboratory_2nd_edn.pdfThe Organisms and Parts that You Use
Species name | Disease risk | Source | Usage |
---|---|---|---|
Escherichia coli (K-12 Derivatives) | None | Loo's Laboratory (in CUHK) | Transformation and DNA extraction |
Azotobacter vinelandii (DJ) | None | Prof. Dennis R Dean's Laboratory | Transformation and DNA extraction |
Aquifex aeolicus | None | Dr. Marianne Guiral's Laboratory | DNA extraction |
Part number/name | Natural function | Usage |
---|---|---|
CFS_EV | Enhancement vector | Enhancement vector for carbon fixation system |
CFS_HAPV | Hydrogenase accessory protein vector | Hydrogenase accessory protein vector for carbon fixation system |
CFS_NAPV | Nitrogenase accessory protein vector | Nitrogenase accessory protein vector for carbon fixation system |
CFS_NHSPV | Nitrogenase and hydrogenase structural protein vector | |
CFS | Functional biobrick for carbon fixation system | |
IES1 | Inducible Expression system biobrick (1) | Intermediate biobrick for Inducible expression system |
IES_ISNE | Specific integration silencing nitrogenase expression | Specific integration silencing nitrogenase expression for inducible expression system |