Team:Evry/Notebook/Sensing/PCBs/08-18-2014

Sensor construction bphR2/PbphR1:

bphR2 gene, synthetized by Eurofins in a plasmid, was dissolved in 23,5µL of elution buffer because it was lyophilized.
Concentration obtained = 200ng/µL

DH5a chimiocompetents were transformed with this plasmid according to this protocol:

1. Remove DH5a from -80°C (about 200µL) and let them on the ice
2. Add 100ng of plasmid at DH5a and let during 30mn on ice
3. Make a heat shock by putting bacteria at 42°C during 30s-1mn
4. Let 1h at 37°C
5. Plate 100µL of the culture on LB-agar-Amp
6. Incubate at 37°C overnight, 200rpm