Team:Evry/Notebook/Sensing/PCBs/08-15-2014

From 2014.igem.org

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Survival test on E.coli BL21:

  1. Serial dilution of compound was made from 10(-2) mol/L to 10(-8) mol/L
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  2. 300µL of E.coli BL21 were added in each eppendorf tube.
    We had 2 control tubes:
    • A negative control which contains 2,7mL of M9 medium + 300µL of compound
    • A positive control which contains 2,7mL of M9 medium + 300µL of E.coli BL21

  3. Tubes were incubated at 37°C overnight, 200rpm

    Aug 15

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