From 2014.igem.org

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6^th June

• We need BL21 E.coli without plasmid for have new competent BL21
  We try a non-selective culture with our bacteria.



• Plasmid extraction by QIAprep Spin Miniprep Kit from QIAGEN of our ELPs.



• Digestion by BamH1 and EcoR1, ligation and transformation by electroporation and by heat shock of our bacteria with low copy IGEM backbone pSB1C3 (2014 Kit Plate 4, well 4B)
  • Transformation by heat shock
    
    1. Plate of LB and pSB1C3
    2. Plate of LB + Chlo and pSB1C3
    3. Plate of LB + Chlo without DNA
    4. Plate of LB + Amp and pSBK^-
  
  
  • Transformation by electroporation: recuperation on
    
    1. Plate of LB and pSB1C3
    2. Plate of LB + Chlo and pSB1C3
    3. Plate of LB + Chlo without DNA
    4. Plate of LB + Amp and pSBK^-