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From 2014.igem.org

16^th June

• We do a new PCR of VPGXG-20, VPGXG-40 and VPGXG-60 with new primers
• Digestion, ligation and transformation of pSB1C3 with our inserts VPGXG-20, VPGXG-40 and VPGXG-60
• Making new ampicillin aliquots : (100 mg/mL)

Dissolve 1 g of sodium ampicillin in sufficient H2O to make a final volume of 10 mL. If sterilization is required, prewash a 0.45- or 0.22-μm sterile filter by drawing through 50-100 mL of H2O. Then pass the ampicillin solution through the washed filter. Store the ampicillin in aliquots at -20°C for 1 year.

• 30mL preculture of LB + Yeast Extract 5g/L + glycerol 1g/L + Amp at 37°C overnight
  • VPGXG-20
  • VPGXG-40
• 30mL preculture of LB + Yeast Extract 5g/L + glucose 1g/L + Amp at 37°C overnight
  • VPGXG-60

• Research in bibliography on consensus sequences of dragline silk