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From 2014.igem.org

16^th July

• SLP gene synthesis
  
  
  PCR Parameter:
  
  98°C 5min
  35 cycles : 98°C 10s, Gradient 68°C delta4°C : (2AB,2AB°,2AB°°,2AB°°°) (3) 67°C 20s ; (2CD,2CD°,2CD°°,2CD°°°) (10) 69°C 20s, 72°C 1min
  Final extension 72°C 10min
  End at 4°C
  
  We obtain this gel
  
  
  
  PCR Parameter:
  98°C 5min
  35 cycles : 98°C 10s, Gradient 63°C delta14°C : (2AB) (6) 66°C 20s ; (2CD) (12) 69°C 20s ; (3 (45)) (9) 66°C 20s ; (3 (primer1,8)) (9) 66°C 20s ; (3(45)(primer1,8)) (9) 66°C 20s, 72°C 1 min
  Final extension 72°C 10min
  End at 4°C
  
  

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