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Team:Aix-Marseille/Parts
From 2014.igem.org
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Part BBa_K1349001
relA
Description
The relA gene encodes a ppGpp synthetase. In bacteria, ppGpp (guanosine 3'-diphosphate 5-' diphosphate) acts as a signaling molecule that regulates a variety of cellular metabolisms in response to changes in the nutritional state of the cells. The relA sequence amplified from E. coli W3110 was mutated by site-directed mutagenesis to remove the PstI site.
Part BBa_K1349002
CusR box
Description
The CusR box allow the binding of the CusR regulator. In E. coli, the CusR/CusS two-component system induces expression of genes involved in metal efflux under condition of elevated Cu concentration. Phosphorylated CusR specifically bind to CusR-dependent promoters.
Part BBa_K1349004
mesh1
Description
Mesh1 is a SpoT orthologs in metazoa. It catalyzes hydrolysis of ppGpp (guanosine 3'-diphosphate 5-' diphosphate).
Other parts
Part BBa_K1349000 - serA_troncated(1-->1008nt)
Part BBa_K1349003 - PcusC
Part BBa_K1349005 - (SH3)x1
Part BBa_K1349006 - cheA
Part BBa_K1349007 - CusR-L-LZA
Part BBa_K1349008 - (SH3pep)-L-LZA
