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From 2014.igem.org

Grew up colonies from transformation plates: Mach I cells with SpyTag- CsgA Only one of the plates really had colonies. Not quite sure why the growth was so small? Plated 100 microliters on each plate

Sent plasmid with SpyTag for sequencing HBD 5- forward Starts right before CsgA in the tet promoter HBD- 7 Starts in the backbone