Team:Peking/JudgingCriteria

Judging Criteria

Through five months of hard work, we have witnessed great development of our project, and we have prepared for the criteria for the Giant Jamboree. We successfully submitted 12 newly designed iGEM Registry Standard parts while using existing iGEM parts as well. We have also investigated the algal bloom in Taihu Lake. Moreover, we have helped several teams including BIT, BNU, and OUC-China, and we received assistance from the ZJU-China and OUC-China. We also popularized iGEM to undergraduates in Peking University campus. In conclusion, we have met all the requirements for an iGEM team, so we believe that we deserve Gold Medal.

Judging form

1.iGEM Registry of Standard Biological Parts

We have designed and constructed 12 brand new BioBrick Parts, and these parts were well documented on the Part Registry pages. The parts are shown as follows:

Table of iGEM Registry of Standard Biological Parts

Part number	Characterisation	Function
BBa_K1378001	MlrA	An enzyme degradating toxin microystin
BBa_K1378003	Microvirin(MVN)	A lectin from Microcystis.aeruginosa
BBa_K1378005	INPNC-linker2-MVN-linker1-mRFP	Fusion protein testing INP direction
BBa_K1378007	INPNC-MVN and constitutively expressing GFP	E.coli-cyanobacteria binding effect testing
BBa_K1378009	INPNC-linker(rTEV)-MVN and constitutively expressing GFP	E.coli-cyanobacteria binding experiments control group
BBa_K1378022	pelB-MlrA	MlrA with a signal peptide from Sec pathway
BBa_K1378023	TorA-MlrA	MlrA with a signal peptide from TAT pathway
BBa_K1378024	MlrA-terminator	Modified MlrA
BBa_K1378025	pelB-MlrA-terminator	Modified pelB-MlrA
BBa_K1378026	TorA-MlrA-terminator	Modified TorA-MlrA
BBa_K1378031	Holin	Hole protein in suicide system
BBa_K1378032	Endolysin	Muralytic enzyme in suicide system

We should emphasize that these de novo parts were built from scratch, and the part BBa_K1378005, BBa_K1378007 and BBa_K1378001 have experimental data available both on our wiki and on part registry pages.

We also used parts BBa_K554009, BBa_K523013, and BBa_K208006 from iGEM Part Distribution and further distribution directly from the Registry. These parts have played important roles in the killing and its improvement parts of our project.

2.Team communication

The significance of team interactions has long been recognized by Peking iGEM and this year our team members and instructors had visited several other teams and was involved in a reginal iGEMers' Meet Up in Wuhan this summer.

Furthermore, we have helped other teams in this summer, including BIT, BNU, and OUC-China. See here. We also received help from ZJU-China that they helped us to transfer specific genes into E. coli’s genome. We also helped two high school students from Beijing Huiwen Middle School and Beijing No.4 High School.

3.Investigation beyond bench

Holding the belief that responsive research lies beyond lab bench, we visited Taihu Lake in July to investigate the algal bloom there. We interviewed the researchers in NIGLAS(Nanjing Institute of Geography and Limnology of the Chinese Academy of Sciences) who monitoring the algal bloom there and we sampled the water in the Taihu Lake and brought these samples back to our lab. See here.

4. Medal Criteria