Team:HokkaidoU Japan/Notebook/H Stem/Replacement To 1A3

From 2014.igem.org

Notebook
Lab Documents

H-Stem Vector Exchange to pSB1A3

  • Start

  • Mini-Prep

    R0010-stem-as-stem-B0015 (on pSB1A3)

  • PCR

    R0010-stem-as-stem-B0015(on pSB1C3) with 100up_F Tm:62.6℃ & 200dn_R Tm:66.6℃

  • Digestion

    pSB1A3 with EcoRI & PstI
    R0010-stem-as-stem-B0015 with EcoRI & PstI

  • Ligation

    Ligate 0010-stem-as-stem-B0015 with pSB1A3

  • Transformation

    R0010-stem-as-stem-B0015(on pSB1A3)
    5.0µL to DH5alpha;

  • Complete!

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