Team:Groningen/Template/MODULE/Notebook/secretion/week3

From 2014.igem.org

August 6 - August 8
 
Obtaining all the BioBricks needed for the secretion system to destroy P. aeruginosa and S. aureus
 
Performing three transformation to E. coli, one with promoter P2 (BBa_I746104), one with lasI (BBa_K081009), and one with aiiA (BBa_C0060)
The tranformated E. coli was divided in several concentrations over LB agar plates with Chloramphenicol
Three glycerol stocks of E. coli DH5α containing the RBS (BBa_ B0034), the double terminator (BBa_ B0015) and the promoter LasR (BBa_ R0079), were inoculated in LB medium, and grown overnight
The plasmids were isolated out of the overnight cultures, and checked on gel
 
 
 
 
 

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