Team:Evry/Notebook/Sensing/PCBs/09-08-2014
From 2014.igem.org
![Picture](https://static.igem.org/mediawiki/2014/0/00/PCB.png)
Sensor construction bphR2/PbpR1
A new tentative of golden gate has been done with modification of volumes of each part:
- J23114-rbs : 0,12µL
- bphR2: 1,05µl
- bphR1:0,31µl
- RFP : 0,64µl
- Terminator: 0,67µl
- pSB1C3,G3: 1µl
The mix enzyme was inchanged and the program is of 8h
Sep 08