Team:Evry/Notebook/Sensing/PCBs/08-27-2014

From 2014.igem.org

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Sensor construction bphR2/PbpR1

Some colonies had grown after the transformation of bphR2 in pSB1C3:

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Products PCR from 26th august has been migrated :



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Expected for the terminator, we have bands at the good tail. So for the terminator, an other PCR has been made but by using the one taq polymerase instead of Q5 DNA polymerase and we obtained the good band:
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Aug 27

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